Set up Protocol for FISH Experiments

Set up protocol for FISH experiments

Comment: Depending on the microscope, the last two wells at the end of the slide may not be possible to use.

Important: label the microscope slide with a pencil!

Project: Date: Slide# and storage: Performer: Comments:

Sample: Type of label: Microscope type: Fixation mode: Image file: FISH protocol: Controls (reference cells and/or reference probes):

Well Sample Cy3 Cy5 Fluos None DNA F Short comment/evaluation*: Nr. red blue green stain %

* Should include comments on for example : Probe signal intensity; Morphotype; Abundance; Associations; Overlap patterns between different probes/stains; Special observations; Comment on comparisons with controls. Note about image- files or other files with more detailed comments.

Drafts for FISH evaluation protocols, Dr. N. M. Lee, 2 May 2015 Evaluation protocol of FISH experiments

Project: Date: Slide# and storage: Performer: Comments:

Sample: Type of label: Microscope type: Fixation mode: FISH protocol: Image file: Controls (reference cells and/or reference probes):

Well number and sample:

Probe Other Photo file comment info Label Cy3 - red Cy5 -blue FLUOS - green DNA stain Probe signal intensity

Morpho- type

Abun- dance

Associ- ations

Overlap patterns

Comp- arisons with controls

Other info

Drafts for FISH evaluation protocols, Dr. N. M. Lee, 2 May 2015 Evaluation of probes for FISH experiments

Project and sample type: Target organisms:

Taxonomical Probe technical information References (hierarchic) rank: Target Probe Probe sequence E. coli Fixation FISH [F] +Hits -Hits Positive Negative Other Publication gene name 5´-3´ (incl competitor) pos. mode protocol % % % reference reference cells cells Domain:

Phylum:

Family

Strain or Clone

Corresponding PCR protocols available?

General comments:

Drafts for FISH evaluation protocols, Dr. N. M. Lee, 2 May 2015