<p> Set up protocol for FISH experiments </p><p>Comment: Depending on the microscope, the last two wells at the end of the slide may not be possible to use.</p><p>Important: label the microscope slide with a pencil!</p><p>Project: Date: Slide# and storage: Performer: Comments: </p><p>Sample: Type of label: Microscope type: Fixation mode: Image file: FISH protocol: Controls (reference cells and/or reference probes): </p><p>Well Sample Cy3 Cy5 Fluos None DNA F Short comment/evaluation*: Nr. red blue green stain %</p><p>* Should include comments on for example : Probe signal intensity; Morphotype; Abundance; Associations; Overlap patterns between different probes/stains; Special observations; Comment on comparisons with controls. Note about image- files or other files with more detailed comments.</p><p>Drafts for FISH evaluation protocols, Dr. N. M. Lee, 2 May 2015 Evaluation protocol of FISH experiments </p><p>Project: Date: Slide# and storage: Performer: Comments: </p><p>Sample: Type of label: Microscope type: Fixation mode: FISH protocol: Image file: Controls (reference cells and/or reference probes): </p><p>Well number and sample: </p><p>Probe Other Photo file comment info Label Cy3 - red Cy5 -blue FLUOS - green DNA stain Probe signal intensity</p><p>Morpho- type</p><p>Abun- dance</p><p>Associ- ations</p><p>Overlap patterns</p><p>Comp- arisons with controls</p><p>Other info</p><p>Drafts for FISH evaluation protocols, Dr. N. M. Lee, 2 May 2015 Evaluation of probes for FISH experiments</p><p>Project and sample type: Target organisms: </p><p>Taxonomical Probe technical information References (hierarchic) rank: Target Probe Probe sequence E. coli Fixation FISH [F] +Hits -Hits Positive Negative Other Publication gene name 5´-3´ (incl competitor) pos. mode protocol % % % reference reference cells cells Domain:</p><p>Phylum:</p><p>Class</p><p>Order</p><p>Family</p><p>Genus</p><p>Strain or Clone</p><p>Corresponding PCR protocols available? </p><p>General comments:</p><p>Drafts for FISH evaluation protocols, Dr. N. M. Lee, 2 May 2015</p>