QX100 Droplet Digital PCR System Pre-Installation Guide

ddPCR Pre-Demo Questionnaire v2.0

Pre-Demo Questionnaire for QX200™ Droplet Digital™ PCR System

Thank you for considering Bio-Rad’s QX200 Droplet Digital PCR System. To ensure a successful demo, please answer the questions below and email the completed form to your sales representative and ddPCR specialist, who are indicated below.

Researcher Contact Information

Name of Institution

Name of Primary Contact

Email Address

Phone Number

Are you interested in bringing your samples and analyze them during the “QX200™ Droplet Digital™ PCR System” hands-on workshop?

Experimental Design

1. What type of application (Copy Number Variation, Rare Event Detection, Abs Quant, SNP detection, etc.) do you want to be performed during the QX200 demonstration?

2. What techniques (PCR, qPCR, Next Gen Sequencing, miRNA array, etc) are you currently using for the above application?

3. What are the key challenges with your current technique for your application?

4. How do you expect ddPCR will improve your results?

Demo Expectations

1. What are you expecting to see demonstrated during the QX200 demonstration?

2. What data or benefits do you need to see in order to acquire this technology?

3. What is your timeframe for the demo and potential purchase of the QX200?

Samples

1. What is the origin of your sample (plant, animal, bacterial, viral, cell line, blood, tissue, FFPE, plasma)?

2. What sample type will be run during the demo (cDNA, gDNA, miRNA, cfDNA, FFPE-treated etc.)?

3. What was your sample prep method (RNAeasy, FFPE-purified, DNAeasy..)?

4. What target(s) would you like us to examine?

5. Do you have positive and negative controls that can be run during the demonstration?

6. What is the concentration of your template (crude estimation, ex. 20ng/ul)?

7. What is the template suspended in (buffer, H2O, TE, low TE (10 mm Tris, 0.1 mM EDTA), etc…) and are there any known additives or inhibitors present?

Current Assay Design

If you have assays that you would like to evaluate, please answer the questions below. Otherwise, please answer the questions for New Assay Design.

1. Did you perform a protocol optimization for this assay (temperature gradient)? If yes, what is the current PCR protocol that is used?

2. What are the primer and probe sequences?

Primer/Probe Name Sequence

3. What is the target sequence? Please also provide at least 100 bases upstream and downstream of the primer binding sites?

4. For TaqMan probe assays, are your probes FAM, VIC or HEX labeled? Probes should be quenched with a dark quencher.

5. Rare Event Detection: Describe the assays specificity for your rare event detection?

6. For CNV assays, what is the reference gene?

7. What qPCR platform are you using with your assay?

New Assay Design

1. What is the target sequence? 2. What is the feature of interest (SNP)? 3. For CNV assays, what is the reference gene?

Customer Data Analysis

1. Are you willing to provide data from your current method to compare with the ddPCR results?

2. What methods do you use to analyze your data? If different from standard ratios, fractional abundance, or reference comparison, please provide some detail.

3. Are you ok with Bio-Rad using the generated data for marketing and conference presentations/posters? Yes or No?