<p> ddPCR Pre-Demo Questionnaire v2.0</p><p>Pre-Demo Questionnaire for QX200™ Droplet Digital™ PCR System</p><p>Thank you for considering Bio-Rad’s QX200 Droplet Digital PCR System. To ensure a successful demo, please answer the questions below and email the completed form to your sales representative and ddPCR specialist, who are indicated below.</p><p>Researcher Contact Information</p><p>Name of Institution</p><p>Name of Primary Contact </p><p>Email Address</p><p>Phone Number</p><p>Are you interested in bringing your samples and analyze them during the “QX200™ Droplet Digital™ PCR System” hands-on workshop?</p><p>YES</p><p>NO</p><p>Experimental Design</p><p>1. What type of application (Copy Number Variation, Rare Event Detection, Abs Quant, SNP detection, etc.) do you want to be performed during the QX200 demonstration? </p><p>2. What techniques (PCR, qPCR, Next Gen Sequencing, miRNA array, etc) are you currently using for the above application?</p><p>3. What are the key challenges with your current technique for your application?</p><p>4. How do you expect ddPCR will improve your results?</p><p>Demo Expectations</p><p>1. What are you expecting to see demonstrated during the QX200 demonstration?</p><p>1</p><p> ddPCR Pre-Demo Questionnaire v2.0</p><p>2. What data or benefits do you need to see in order to acquire this technology?</p><p>3. What is your timeframe for the demo and potential purchase of the QX200?</p><p>Samples</p><p>1. What is the origin of your sample (plant, animal, bacterial, viral, cell line, blood, tissue, FFPE, plasma)?</p><p>2. What sample type will be run during the demo (cDNA, gDNA, miRNA, cfDNA, FFPE-treated etc.)?</p><p>3. What was your sample prep method (RNAeasy, FFPE-purified, DNAeasy..)?</p><p>4. What target(s) would you like us to examine? </p><p>5. Do you have positive and negative controls that can be run during the demonstration?</p><p>6. What is the concentration of your template (crude estimation, ex. 20ng/ul)?</p><p>7. What is the template suspended in (buffer, H2O, TE, low TE (10 mm Tris, 0.1 mM EDTA), etc…) and are there any known additives or inhibitors present?</p><p>Current Assay Design</p><p>If you have assays that you would like to evaluate, please answer the questions below. Otherwise, please answer the questions for New Assay Design.</p><p>1. Did you perform a protocol optimization for this assay (temperature gradient)? If yes, what is the current PCR protocol that is used?</p><p>2</p><p> ddPCR Pre-Demo Questionnaire v2.0</p><p>2. What are the primer and probe sequences?</p><p>Primer/Probe Name Sequence</p><p>3. What is the target sequence? Please also provide at least 100 bases upstream and downstream of the primer binding sites?</p><p>4. For TaqMan probe assays, are your probes FAM, VIC or HEX labeled? Probes should be quenched with a dark quencher.</p><p>5. Rare Event Detection: Describe the assays specificity for your rare event detection?</p><p>6. For CNV assays, what is the reference gene?</p><p>7. What qPCR platform are you using with your assay? </p><p>New Assay Design</p><p>1. What is the target sequence? 2. What is the feature of interest (SNP)? 3. For CNV assays, what is the reference gene?</p><p>Customer Data Analysis</p><p>1. Are you willing to provide data from your current method to compare with the ddPCR results?</p><p>2. What methods do you use to analyze your data? If different from standard ratios, fractional abundance, or reference comparison, please provide some detail.</p><p>3. Are you ok with Bio-Rad using the generated data for marketing and conference presentations/posters? Yes or No? </p><p>3</p>
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