PCB Lab Write-Ups

PCB lab write-ups: Useful information:

1. Calculate concentrations of PCBs PCBs 1-3 = monochloro in the air of New Brunswick and 4-15 = dichloro PUT IN CONTEXT. Compare 16-39 = trichloro indoor and outdoor 40-81 = tetra concentrations and congener 82-127 = penta patterns. Are they different? 128-169 = hexa 2. Calculate breakthrough of each 170-193 = hepta PCB congener/chromatographic 194-205 = octa peak using the split PUF data and 206-208 = nona this formula: 209 = deca

%Breakthrough = (mass on Concentration of PCBs 30 and 204 in the bottom PUF)/(total mass top+ Internal Standard spike solution is 200 bottom PUF) *100% ng/mL each Concentration of PCBs 14, 23, 66, 165 What does the level of in the surrogate spike solution is 210 breakthrough say about our ng/mL each results? 3. Compare mass of PCBs on lab Surrogate PCBs: and field blanks to samples. Is PCB 14 = 3,5-dichloro there a need to subtract blank PCB 23 = 2,3,5-trichloro masses from samples? PCB 65 = 2,3,5,6-tetrachloro 4. Calculate % recovery of all PCB 166 = 2,3,4,4’,5,6-hexachloro congeners for all matrix spikes. Are the recoveries good? Internal Standard PCBs: Acceptable? PCB 30 = 2,4,6-trichloro 5. Calculate surrogate recoveries PCB 204 = 2,2',3,4,4',5,6,6'-octachloro for all samples, MS, and blanks and explain whether our results GC conditions: should be corrected for them. Are the recoveries good? 6890 Agilent Gas Chromatograph Acceptable? Column = 60 m DB-5 0.25 um film thickness, 0.32 mm internal diameter.

Injector : splitless at 285C

Detector: ECD at 310C

Temperature program: start at 70C, ramp 7 /min to 180 then 1.05 /min to 225 then 5.75 /min to 285 then 11.5 /min to 300C. hold at 300C for 8 minutes