Supplementary material

Table S1. Nei genetic distances among pairs of populations of Cactoblastis cactorum in the Caribbean and Florida.

Pal Isla isa Muj do Antigua Navis LaRomana Trinidad Pinar Santiago LasTablas Guanica eres Atlantic Gulf res Antigua 0 0.00012 Nevis 88 0 0.00145 LaRomana 69 0.002453 0 0.00015 Trinidad 5 0.0002205 0.001825 0 0.00328 Pinar 61 0.0037249 0.0037003 0.0021644 0 0.00195 0.0003 Santiago 86 0.0025603 0.0018252 0.0012812 271 0 0.00378 0.0000 0.000584 LasTablas 92 0.0041465 0.0044774 0.0025246 382 2 0 0.00200 0.0040 0.002097 Guanica 47 0.0031512 0.0000435 0.0024097 675 6 0.004892 0 0.00378 0.0000 0.000584 IslaMujeres 92 0.0041465 0.0044774 0.0025246 382 2 0 0.004892 0 0.00 0.00061 0.0024 0.000996 300 Atlantic 04 0.0012732 0.0002722 0.0007124 085 7 0.0030033 0.0005091 33 0 0.00 0.00338 0.0000 0.000372 002 0.002521 Gulf 06 0.0038031 0.0038496 0.0022304 015 4 0.0000245 0.0042263 45 3 0 0.00 0.00014 0.0037 0.002572 415 0.001284 Palisadores 03 0.0000114 0.0024648 0.0002321 368 2 0.0041584 0.0031631 84 9 0.003815 0

Pali sad LasTabla Guan IslaMujer ore Antigua Navis LaRomana Trinidad Pinar Santiago s ica es Atlantic Gulf s Antigua 0 Nevis 88.442 0 LaRoman a 807.448 719.008 0 2035.62 Trinidad 9 1947.208 1228.711 0 2473.64 Pinar 1 2385.282 1667.629 440.441 0 1553.66 Santiago 7 1465.235 746.596 482.121 921.568 0 LasTabla s 955.369 866.941 149.378 1080.278 1518.772 598.306 0 Guanica 568.081 479.641 239.367 1467.869 1906.46 985.794 387.694 0 IslaMujer 2776.40 2209. es 5 2688.114 1971.263 745.46 305.136 1225.879 1822.204 795 0 2177.93 1610. Atlantic 8 2089.636 1371.975 213.201 369.752 636.955 1226.925 738 656.655 0 2370. Gulf 2937.62 2849.294 2131.963 904.632 464.443 1386.011 1983.028 693 162.36 803.079 0 Palisador 1669.20 1102. 1107.24 es 4 1580.893 864.503 370.819 804.7 130.722 715.253 66 3 547.737 1268.426 0 Table S2. Euclidean geographic distances between pairs of populations of Cactoblastis cactorum in the Caribbean and Florida.

Table S3. Connectivity matrix between all pairs of populations of populations of Cactoblastis cactorum in the Caribbean and Florida.

Antigua Navis LaRoman Trinidad Pinar Santiago LasTabla Gua IslaMuje Atlantic Gulf Pa lis ad or a s nica res es Antigua 0 0.01811 Nevis 5 0 LaRoma 0.04000 na 9 0.038899 0 0.05707 Trinidad 1 0.056143 0.052063 0 0.05399 Pinar 4 0.053068 0.049049 0.03258 0 0.05620 Santiago 1 0.055252 0.050424 0.046676 0.044547 0 LasTabla 0.04491 s 2 0.043853 0.032654 0.052915 0.049929 0.050733 0 0.03435 Guanica 4 0.033058 0.032421 0.05348 0.050429 0.052274 0.038856 0 0.05 IslaMuje 0.05959 602 res 4 0.058668 0.054652 0.039013 0.025884 0.050239 0.055529 9 0 0.09 0.09962 632 0.08398 Atlantic 3 0.098716 0.095212 0.085475 0.079146 0.093262 0.096474 8 6 0 0.07 0.08308 959 Gulf 2 0.082162 0.078293 0.064083 0.053552 0.074569 0.07929 6 0.05559 0.101495 0 Palisador 0.06484 0.06 0.05555 es 3 0.063888 0.059055 0.052997 0.050006 0.050637 0.059223 087 1 0.100954 0.080852 0