Table S1. Information About Human DPP-4 Protein and Its Gene (Derived from the NCBI Data Bank)

Table S1. Information about human DPP-4 protein and its gene (derived from the NCBI data bank)

Protein/ gene ID Other Information References

Protein GenBank: ACCESSION: AAA52308 [1, 2] AAA52308.1 VERSION: AAA52308.1 GI:181570 Length of the protein: 766 aa

Gene Gene ID: 1803, Location: 2q24.3 [3] updated on 3-Mar- Similar name: CD26; ADABP; 2013 ADCP2; DPPIV; TP103

References: 1.Darmoul D, Lacasa M, Baricault L, Marguet D, Sapin C, Trotot P, Barbat A, Trugnan G. Dipeptidyl peptidase IV (CD 26) gene expression in enterocyte-like colon cancer cell lines HT-29 and Caco-2. Cloning of the complete human coding sequence and changes of dipeptidyl peptidase IV mRNA levels during cell differentiation. J Biol Chem. 1992 Mar 5;267(7):4824-33.

2. Broxmeyer HE, Hoggatt J, O'Leary HA, Mantel C, Chitteti BR, Cooper S, Messina-Graham S, Hangoc G, Farag S, Rohrabaugh SL, Ou X, Speth J, Pelus LM, Srour EF, Campbell TB. Dipeptidylpeptidase 4 negatively regulates colony-stimulating factor activity and stress hematopoiesis. Nat Med. 2012 Dec;18(12):1786-96

3. Sun AL, Deng JT, Guan GJ, Chen SH, Liu YT, Cheng J, Li ZW, Zhuang XH, Sun FD, Deng HP. Dipeptidyl peptidase-IV is a potential molecular biomarker in diabetic kidney disease.Diab Vasc Dis Res. 2012 Oct;9(4):301-8

Table S2. DPP-4: molecular weight, high scoring hydrophobic segments, and high scoring transmembrane segments Protein Mol. Compositional analysis of High scoring hydrophobic High scoring W. amino acids segments transmembrane (kD) segments CRP ~ 88.3 A : 40( 5.2%); C : 12( 1.6%); 2.00 (LVIFM) 5.00 (LVIF) D : 45( 5.9%); E : 41( 5.4%); F 1.00 (AGYCW) 2.00 (AGM) : 31( 4.0%)G : 43( 5.6%); 0.00 (BZX) 0.00 (BZX) H : 19( 2.5%); I : 50( 6.5%); -2.00 (PH) -1.00 (YCW) K : 39( 5.1%); L : 62( 8.1%) -4.00 (STNQ) -2.00 (ST) M : 15( 2.0%); N : 40( 5.2%); P -8.00 (KEDR) -6.00 (P) : 29( 3.8%); Q : 30( 3.9%); -8.00 (H) R : 31( 4.0%);S : 64( 8.4%); -10.00 (NQ) T : 49( 6.4%); V : 49( 6.4%); -16.00 (KR) W : 21( 2.7%); Y++: 56( 7.3%) -17.00 (ED)

Table S3- Prediction of the cysteines position and the probable disulphide bonds of DPP-4

Cysteines predicted at respective positions Disulphide bonds by decreasing Bond order of probability Cysteine Cysteine Index position 1 position 2

1 385 394 Total number of cysteines: 12 2 328 339 Predicted number of bonds: 5 3 444 447 Cysteines at the following positions are predicted to 4 form the disulfide bond: 454 472 5 328,339,385,394,444,447,454,472,649,762 649 762

Table S4. The corresponding N-glycosylation and O-glycosylation sites with their relative information (A) Relative information about N-glycosylation (B) Relative information about O-glycosylation