Department of General Microbiology

Laboratory: Microbial Biochemistry

Head: Assoc. Prof. Danka Galabova, PhD

Tel. +359 2 979 31 30

[email protected]

STAFF:

Tatyana Avramova, PhD, Assoc. Prof. – 359 2 979 31 07, [email protected]

Blaga Angelova, PhD, Assoc. Prof. – 359 2 979 31 54, blaga @microbio.bas.bg

Evgenia Vasileva-Tonkova, PhD, Assoc.Prof. – 359 2 979 31 39, [email protected]

Nelly Christova PhD, assist. prof.– 359 2 979 31 15, [email protected]

Anna Sotirova, MS, assist. prof. – 359 2 979 31 39, anna@ microbio.bas.bg

Mimi Remichkova, PhD, assist. prof. – 359 2 979 31 15, [email protected]

Stephan Engibarov, MS, assist. prof. – 359 2 979 31 47, [email protected]

Rumyana Eneva, MS, Research Assis.t– 359 2 979 31 47, [email protected]

Irina Lazarkevich, MS, Research Assist.- 359 2 979 31 39, [email protected]

Research topics

Microbial enzymes: neuraminidases, phosphatases, protein phosphatases, proteases, nucleases – mechanism of secretion, isolation, purification, properties, biological role. Microbial transformation of steroid compounds. Antimicrobial agents – esters of thiosulfanilic acid and their effect on model pathogen bacteria. Molecular analysis of microbial virulence factors.Microbial degradation of aliphatic and aromatic hydrocarbons – environmental pollutants. Biochemistry and microbiology of anaerobic digestion. Microbial surfactants (biosurfactants) – biosynthesis, isolation, purification, physical chemical properties; effect on microbial cell surface and properties; application in medical, pharmacological and environmental biotechnologies. Current Projects

 Influence of biosurfactants and their complexes with esters of thiosulfanilic acid on bacteria and viruses. Perspectives for applications in medical, pharmacological and environmental biotechnologies. NSF, Republic of Bulgaria;

 Microbial transformation of steroids within a system of micro channels. NSF, Republic of Bulgaria

 Bacterial surfactants– biotechnology, characterization and application. NSF, Republic of Bulgaria;

 Effect of newly synthesized complex preparations with surface active and biocide properties on pathogenic bacteria and viruses. Role of biosurfactants in processes of anaerobic digestion of mixture of organic wastes. Bilateral Cooperation between the Bulgarian Academy of Sciences and Ukrainian Academy of Sciences;

 Implementation of microreactor technology and ionic liquid for the development of rational and sustainable biotransformation / biodegradation processes. Bilateral Cooperation between the Bulgarian Academy of Sciences and the Slovenian Academy of Sciences;

References:

 Angelova, B., P. Fernandes, D. Spasova, S. Mutafov, H.M. Pinheiro, Cabral J.M.S., 2006, Scanning electron microscopy investigations on bis(2-ethylhexyl)phthalate treated Mycobacterium cells. Microscopy Research Technique, 69, 613-617.

 Vasileva-Tonkova, E., D. Galabova, E. Stoimenova, Lalchev Z., 2006, Characterization of bacterial isolates from industrial wastewater according to probable modes of hexadecane uptake. Microbiol. Research, 163 (4), 481-486.

 Vasileva-Tonkova, E., D. Galabova, E. Stoimenova, Lalchev Z., 2006, Production and properties of biosurfactants from a newly isolated Pseudomonas fluorescens HW-6 growing on hexadecane, Z. Naturforsch., 61c, 553-559.  Abrashev, I., Orozova P., 2006, E. rhusiopathiae neuraminidase and its role in pathogenicity. Z. Naturforsch., 61c, 434-438.

 Avramova, T., L. Stefanova, B. Angelova, Mutafov S., 2007, Bacterial decolorization of Acid Orange 7 in the presence of ionic and non-ionic surfactants. Z. Naturforsch., 62 (1-2), 87-92.

 Mutafov, S., T. Avramova, L. Stefanova, Angelova B., 2007, Decolorization of Acid Orange 7 by bacteria of different tinctorial type: a comparative study. World J. Microbiol. Biotechnol., 23 (3), pp. 417-422

 Tuleva, B., Christova, N., Cohen, R., Stoev, G., Stoineva I., 2008, Production and structural elucidation of trehalose tetraesters (biosurfactants) from a novel alkanothrophic Rhodococcus wratislaviensis strain. J. Appl. Microbiol.,104, 1703- 1710.

 Vasileva-Tonkova, E., Galabova, D., Stoimenova, E., Lalchev, Z., 2008, Characterization of bacterial isolates from industrial wastewater according to probable modes of hexadecane uptake. Microbiol. Res., 163, 481—486.

 Remichkova, M., Galabova, D., Roeva, I., Karpenko, E., Shulga, A., Galabov, A., 2008, Anti-Herpesvirus Activities of Pseudomonas sp. S-17 Rhamnolipid and its Complex with Alginate. Z. Naturforsch., 63c, 75-81.

 Sotirova, A., Spasova, D., Galabova, D., Karpenko, E., Shulga, A., 2008, Rhamnolipid–biosurfactant permeabilizing effects on Gram-positive and Gram- negative bacterial strains. Curr. Microbiol., 56, 639-644,.

 Mutafov, S., Avramova, T., Angelova, B., Stefanova, L., 2008, Temperature effect on the azo bond reduction kinetics of a sulfonated mono azo dye. Biodegradation., 19, 387-389.

 Avramova, T., Sotirova, A., Galabova, D., Karpenko, E., 2008, Effect of Triton X-100 and rhamnolipid PS-17 on the mineralization of phenanthrene by Pseudomonas sp. cells. Intern.Biodeter.Biodegr., 62, 415-420.

 Tuleva, B., Christova, N., Cohen, R., Antonova, R., Todorov, T., Stoineva, I., 2008, Isolaton and charaterization of trehalose tetraester biosurfactants from a soil strain Micrococcus luteus BN56. Process Biochem., 44 (2), 135-141.  Stoimenova, E., Vasileva-Tonkova, E., Sotirova, A., Galabova, D., Lalchev, Z., 2009, Evaluation of Different Carbon Sources for Growth and Biosurfactant Production by Pseudomonas fluorescens Isolated from Wastewaters., Z. Naturforsch. 64 c, 96 – 102.

 Sotirova, A., D. Spasova, E. Vasileva-Tonkova, D. Galabova, 2009, Effects of rhamnolipid- biosurfactant on cell surface of Pseudomonas aeruginosa. Microbiol. Research, 164 (3), 297-303.

 Tuleva, N. Christova, R. Cohen, D. Antonova, Tihomir Todorov, Stoineva I., 2009, Isolaton and charaterization of trehalose tetraester biosurfactants from a soil strain Micrococcus luteus BN56., Process Biochemistry, 44, 135-141.

 Avramova T, Spassova D, Mutafov S, Momchilova S, Boyadjieva L, Damyanova B, Angelova B., 2010, Effect of Tween 80 on 9α-steroid hydroxylating activity and ultrastructural characteristics of Rhodococcus sp. cells., World J. Microbiol., 26 (6), 1009-1014.

 Абрашев И., Енгибаров Ст., Енева Р., 2010, Vibrio cholerae -биология и екология. Екологично Инженерство и Опазване на Околната Среда, №1,42-52.

 Sotirova A., Avramova T., Lazarkevich I., Lubenets V., Karpenko E., Galabova D., 2010, Antimicrobial potential of selected thiosulfonates - based biocides and biosurfactants against bacteria and fungi., Comptes rendus de l’Académie bulgare des Sciences, 63, 9, 1304-1314.

 Spasova D., Sotirova A., Avramova T., Galabova D., 2010, Effect of rhamnolipid- biosurfactant on the ultrastructure of Pseudomonas aeruginosa. Comptes rendus de l’Académie bulgare des Sciences , 63,10, 1449-1454.

 Tishinov K. , Christov P., Neshev G., Nustorova M., Paskaleva D., Vasileva-Tonkova E., Gousterova A., Nedkov P., 2010, Investigation of the possibility for enzymatic utlization of chicken bones. Biotechnology and Biotechnological Equipment, 24, 4, 2108-2111.

 Vasileva-Tonkova, E., Sotirova, A., Galabova, D. 2011. The effect of rhamnolipid biosurfactant produced by Pseudomonas fluorescens on model bacterial strains and isolates from industrial wastewater. Current Microbiology 62 (2), pp. 427-433