Preparations of various Amphotericin B formulations (non-sterile) (Adapted from Dr. Turtinen’s method)

Fungizone (Amphocin)

1. Allow to come to room temperature in dark place (pocket). 2. On quantitative balance, weigh out 11.1 mg using weigh paper (keep lights off/low). 3. Place in microfuge tube and add 1mL of buffer or water right before use. Keep in the dark.

a. This corresponds to 5mg/mL (5.41mM) of Amphotericin B

Ambisome

1. Allow to come to room temperature in dark place (pocket). 2. On quantitative balance, weigh out 136 mg using weigh paper (keep lights off/low). 3. Place in microfuge tube and add 1mL of buffer or water right before use. Keep in the dark.

a. This corresponds to 5mg/mL (5.41mM) of Amphotericin B.

Amphotec

1. Allow to come to room temperature in dark place (pocket). 2. On quantitative balance, weigh out 103 mg using weigh paper (keep lights off/low). 3. Place in microfuge tube and add 1mL of buffer or water right before use. Keep in the dark.

a. This corresponds to 5mg/mL (5.41mM) of Amphotericin B.

Abelcet

1. Keep in fridge until needed 2. The concentration of this suspension is 5mg/mL (5.41mM) of Amphotericin B. Keep in the dark.