Safe Operating Procedures for the Use of Centrifuges

Safe Operating Procedures for the Use of Centrifuges: Doc # SS-SOP#2 Date 15.07.13

Hazards Associated with Centrifuges:  Contact with rotating parts.  Mechanical failure of rotating parts.  Sample leaks causing aerosols, stress corrosion and contamination.  Sample imbalance causing machine movement.  Fire / explosion.  Contact with contaminated components / vapours.

All users of centrifuges MUST be trained by their supervisor or other appointed person before using a centrifuge. This training MUST be documented.

Centrifuges are potentially lethal therefore you must follow the below Safe Operating Procedures.  Only suitably trained personnel may operate a centrifuge.  Please ensure that the centrifuge log book is filled in.  Before using the rotor, please check the rotor, its lid and O-ring / seals for cleanliness and damage. Damaged rotors MUST NOT be used.  If you are to use a swing-out rotor please ensure that the tubes you use clear the rotor in the full swing out position.  Please check the quality of the centrifuge tubes. If damaged in any way, DO NOT use.  DO NOT fill the centrifuge tubes above the recommended volume.  Ensure that the centrifuge tube is compatible with any solvents that you may be using.  DO NOT spin the centrifuge tubes above the maximum stated speed as recommended by the manufacturer of the tubes.  Centrifuge tubes MUST be balanced to balance the rotor.  Ensure that the correct lid is placed on the rotor.  You MUST stay with the centrifuge until it gets up to speed. If any problem occurs TURN IT OFF IMMEDIATELY.  For the Beckman Centrifuges you are required to enter the “Rotor Type”. Ensure that you enter the correct type e.g. for JA 21 rotor you would enter 21.  Any Spills in the rotor or the centrifuge MUST be cleaned up immediately.  Explosive or highly flammable chemicals should not be used in a centrifuge.  You MUST wait until the rotor is completely stopped before opening the lid and touching the rotor.  Only authorized personnel may service or repair the centrifuge.  Immediately report any centrifuge faults to Biomedical Engineering 64052, and place a sign on the centrifuge documenting the problem.  For training please contact Angela Binns, – ext. 68995, pager 2199

Mrs. Angela Binns Assistant Manager of Support Services, School of medicine 15.07.13