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Supplemental Material Fold Change Symbol Genbank Description UniGene HNS expression Hyper-osmotic regulation Hypo-osmotic regulation 13.67 Giot1 NM_133563 Gonadotropin inducible ovarian transcription factor 1 (Giot1) Rn.107259 Yue et al., 2006 5.223 Fosl1 NM_012953 Fos-like antigen 1, Fra1 Rn.11306 Sharp et al., 1991 Miyata et al., 2001 4.343 c-fos BF415939 c-fos oncogene Rn.103750 Carter and Murphy, 1990 Yue et al., 2006 Sharp et al., 1991 Mutsuga et al., 2005 Luckman et al., 1996 Miyata et al., 2001b Cunningham et al., 2004 Ji et al., 2005; Kawasaki et al., 2005 Penny et al., 2005 Gottlieb et al., 2006 4.155 Creb3l1 BI304123 cAMP responsive element binding protein 3-like 1 Rn.24541 2.588 Cebpb NM_024125 CCAAT/enhancer binding protein (C/EBP), beta Rn.6479 2.282 Nr4a1 NM_024388 Nuclear receptor subfamily 4, group A, member 1 Rn.10000 Xiao et al., 1996 Luckman, 1997 Mutsuga et al., 2005 Kawasaki et al., 2005 2.268 Atf4 NM_024403 Activating transcription factor 4 Rn.2423 Yue et al., 2006 1.999 Crem NM_017334 cAMP responsive element modulator Rn.10251 Kell et al., 2004 Mellstrom et al., 1993 Luckman and Cox, 1995 1.93 Crem AW529408 cAMP responsive element modulator Rn.10251 Kell et al., 2004 Mellstrom et al., 1993 Luckman and Cox, 1995 1.722 Giot2 AB047638 Gonadotropin inducible ovarian transcription factor 2 (Giot2); Zfp347 Rn.127808 1.687 Ddit3 NM_024134 DNA-damage inducible transcript 3 Rn.11183 1.641 Myc NM_012603 c-myc Rn.12072 1.556 Nfe2l1 AI179123 Nuclear factor, erythroid derived 2,-like 1 Rn.167338 Mutsuga et al., 2005 1.553 Atf5 BM391471 Activating transcription factor 5 Rn.195729 1.467 Mlx AI549299 Max-ilke protein X Rn.16914 1.432 E2f6 AA850881 E2F transcription factor 6 Rn.4132 1.312 Mlx BI278563 Max-like protein X Rn.16914 1.293 Btf3 BF408064 Basic transcription factor 3 Rn.110195 1.274 Hif1a NM_024359 Hypoxia inducible factor 1, alpha subunit Rn.10852 1.272 Ets1 NM_012555 c-ets 1 Rn.88756 1.252 Atf7ip AA819758 Activating transcription factor 7 interacting protein; ATFa-associated Modulator Rn.98469 1.25 Nrbf2 NM_022186 Nuclear receptor binding factor 2 Rn.44867 1.224 Jun NM_021835 c-jun Rn.93714 Carter and Murphy, 1990 Mutsuga et al., 2005 Luckman et al., 1996 Kawasaki et al., 2005 1.223 Gtf2e2 AA996974 General transcription factor II E, polypeptide 2 (beta subunit) Rn.103659 1.222 Taf9 AI172041 TAF9 RNA polymerase II, TATA box binding protein (TBP)-associated factor Rn.8139 1.218 Phtf1 BE106772 Putative homeodomain transcription factor 1 Rn.22952 1.211 Polr2g NM_053948 RNA polymerase II (DNA directed) polypeptide G Rn.106248 1.189 Jund BE329377 JunD 1.151 rKr1 U41164 Cys2/His2 zinc finger protein (rKr1); Zfp180 Rn.14669 1.112 Gtf2f1 BF420770 General transcription factor IIF polypeptide 1 (74kD subunit) Rn.98899 0.853 Zfp96 AW531684 Zinc finger protein 96 Rn.44961 0.818 Nr3c1 NM_012576 Nuclear receptor subfamily 3, group C, member 1 Rn.90070 0.72 Zbtb20 BF415114 Zinc finger and BTB domain containing 20 Mutsuga et al., 2005 0.756 Bbx BF392234 HMG-BOX transcription factor bobby sox Rn.113000 0.741 Smarca3 BM384827 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 3 Rn.161919 0.722 Hod NM_133621 Homeobox only domain Rn.2989 0.696 Nr1d1 M25804 Nuclear receptor subfamily 1, group D, member 1 Rn.29848 0.684 Nr1d2 BF284190 Nuclear receptor subfamily 1, group D, member 2 Rn.10055 Mutsuga et al., 2005 0.631 Nr3c2 NM_013131 Nuclear receptor subfamily 3, group C, member 2 Rn.9678 0.598 Nr1d2 U20796 Nuclear receptor subfamily 1, group D, member 2 Rn.10055 Mutsuga et al., 2005 0.553 Dbp AI230048 D site albumin promoter binding protein Rn.11274 Supplemental Table 3. Transcription factor transcripts significantly regulated by 3 days of fluid deprivation in the male rat supraoptic nucleus. Some of these transcription factors have previously been described as being expressed in the HNS (HNS expression). In addition, some of the transcripts identified are already known to be regulated at the level of their steady-state abundance by a hyper-osmotic stimulus (Hyper-osmotic regulation) or by a hypo-osmotic stimulus (Hypo-osmotic regulation) .
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