Sheldon Biotechnology Centre

Mass Spectroscopy Sheldon Biotechnology Centre Application of mass spectroscopy ionization technologies: fast atom (MS) to protein characterization is bombardment (FAB)and electro- The Sheldon Biotechnology Centre ment and university to meet and a relatively new field. Several spray (ES) ionizations. FAB-MSis selVes as the core facility for discuss mutual interests in the field. applications have been realized useful in the low mass range and biotechnology research at McGill Recent speakers have included and are routinely available, how- may provide fragmentation infor- University. We have state-of-the-art Professor Luc Montagnier, Pasteur ever many new applications are yet mation. ES-MSis a milder ioniza- technical support facilities and Institute whose topic was to be realized. offer reliable low cost selVice to tion technique and allows larger "Biotechnology in AIDS Diagnosis molecules (up to 100,000 amu) to investigators. and Prevention"; Dr. Webster K. Mass spectroscopy is readily applied be characterized. Sample analysis to quality control of synthetic SelVices available at the Sheldon Cavenee, Director, Ludwig Institute and data evaluation are provided for Cancer Research whose topic peptides, characterization of whole for both technologies. Biotechnology Centre are oligo- was "Molecular Genetics of Human protein digests, identification of nucleotide synthesis/purification, Cancer" and Dr. Leroy E. Hood, post-translational modifications, etc. Emphasis is placed on assessing peptide synthesis, protein/peptide Director, Science and Technology the protein related requirement sequencing, protein/peptide amino The Sheldon Biotechnology Centre Center for Biotechnology whose and providing guidance for appro- acid analysis and mass spectroscopy. has access to two mass spectrom- priate mass spectral analysis. topic was "Biotechnology, the eters which employ different' We are dedicated to remaining in Genome Initiative and Medicine in the forefront of recombinant DNA the Twenty-first Century". We strive methodology, protein sequencing to maintain a high standard of analysis and protein separations. internationally recognized speakers. Ordering This objective will selVe investigators undertaking new studies and All work is performed upon receipt in the transfer of information Teaching Program of a valid purchase order. To obtain in Biotechnology between various scientific disciplines. order forms and price lists, please The Minor Program in Biotech- contact the Sheldon Biotechnology Collaborative Program nology is administered for the Centre by telephone (514) 398-3998 The Sheldon Biotechnology Centre Faculties of Engineering, Medicine, or by fax (514) 398-8069. represents McGill University and its Agriculture and Science by the Telephone orders can be placed affiliated hospitals and research Department of Biology. This Monday through Friday during institutes to the larger community Program allows students to select a regular business hours. Fax orders on matters relating to biotechno- package of courses that will can be placed 24 hours a day. broaden their perspective in areas Acknowledgements logy. We recognize the need to The staff of the Sheldon form strong relationships with impacting on biotechnology. Biotechnology Centre respects The Sheldon Biotechnology Centre colleagues in industry. Our Biotech- Biotechnology 505B is a key acknowledges the following for nology Collaborative Program the confidentiality of research component of the Minor Program information and projects. their support: Dr. Huntingdon facilitates and coordinates the in Biotechnology and an important Sheldon; Pharmacia Canada Inc.; development of joint ventures course for graduate students For more information: Hewlett-Packard Canada; the Deans between the business community seeking to expand their knowledge of the Faculties of Science, Medicine, and McGill researchers working in in biotechnology. This multidisci- SHELDON BIOTECHNOLOGY CENTRE Agriculture and Graduate Studies the field of biotechnology. plinary course is taught by out- McGill University and Research of McGill University; standing McGill professors and 3773 University Street the Research Institutes of the Victor L. Cape Lecture Series Montreal, Quebec H3A 2B4 researchers from industry. Each Canada Montreal Children's Hospital and The focus of this series is on lecturer presents the significance of Tel.: (514) 398-3998 the Royal Victoria Hospital. The biotechnology. The program pro- his or her own research to the field Fax: (514) 398-8069 Sheldon Biotechnology Centre has vides a forum for industry, govern- of biotechnology. or return the attached card, also received MRC/NSERCEquip- indicating your area of interest. ment and Salary Support Grants. ,-

Oligonucleotide Synthesis/Purification Protein/Peptide Sequencing Synthetic oligonucleotides are (FPLC) give you complete assur- I'rotdn/pt:ptidt: st:quencing assists At the Sheldon Biotechnology Centre essential reagents to the molecular ance of successful synthesis, high investigators with separation we use Porton Protein/Peptide biologist and are used in a wide yield and purity. methods and physical analysis of MicroSequencers with an integrated proteins. Sample purity and quan- customized microbore HPLC to variety of recombinant DNA manip- Oligonucleotides are cleaved and titation are critical parameters optimize sequence. Direct on-line ulations, including DNA sequenc- deprotected at 550C for 16 hours ing, PCR amplification, site-directed in concentrated ammonium which strongly influence data analysis allows us to sequence mutagenesis, etc. assessment and reliability particu- picomole amounts of protein with hydroxide. Purification by FPLC lady at the picomole level. exceptional results. The Sheldon Biotechnology Centre can be completed within 24 to 48 has capabilities for high throughput hours following deprotection. This facility serves as the resource Our protein/peptide sequencing for peptide and protein isolation facility provides direct sequence and flexibility. Using the Gene The Sheldon Biotechnology Centre strategies using classical as well as analysis of isolated peptide and Assemble~ Plus, a 2 column DNA is the source for affordable syn- high performance liquid chromatog- protein fragments and interpretation synthesizer, we can provide you thetic oligonucleotides resulting in raphy procedures. of the amino acid sequence data. with oligonucleotides of lengths to considerable savings to investigators. over 100 bases that include regular or modified bases such as inosine All oligonucleotides are guaran- We provide advice in the prepara- High quality reliable data routinely teed. Should there be a problem, tion of samples for sequencing and obtained by the Porton Gas Phase or methylated derivatives. On-line we will re-synthesize and/or re- in methods of purification to those Sequencer is evaluated by comput- monitoring during automated purify without charge. unfamiliar with the techniques of erized techniques and by an expe- synthesis and purification by Fast protein chemistry. We strongly rienced sequencer. Qualification of Protein Liquid Chromatography recommend consultation with the the results with respect to sample Facility Manager regarding compat- purity and quantitation is empha- ibility of your protein/peptide sized when reported to the client. purification protocol with high sen- Peptide Synthesis sitivity protein/peptide sequencing. A very broad range of applications deprotected peptide is assessed require synthetic peptides. Peptide using the HPLC DfB System Two Protein/Peptide Amino Acid Analysis synthesis can be time consuming which is equipped with a Rapid without specialized scientists and Spectral Detector. Depending on Amino acid analysis is one of the advantage where samples are not proper equipment. the sequence, the purity of the most exacting application areas of pure proteins but contain many crude peptide varies between 70% liquid chromatography. Results other components. Hydrolysis At the Sheldon Biotechnology have to be both accurate and Centre we have state of the art and 95%. If your application time-course profiles and/or pre- requires greater purity, we offer reproducible. hydrolysis chemical modifications technology and peptide chemists complete purification and charac- are available for exacting quantita- that guarantee the synthesis. Solid At the Sheldon Biotechnology tion of residues modified, destroyed terization services. We have capa- Centre, we use the Alpha Plus phase peptide synthesis is carried bilities for carrying out quality or slowly released during routine out on the Biolynx™ Peptide control at the level of amino acid Amino Acid Analyzer. With this hydrolysis conditions. system, the amino acids of the most Synthesizer which uses the Fmoc analysis, mass spectroscopy and complex protein hydrolysates or For complex biological fluids, we polyamide peptide synthesis lO-peptide sequencing, depending physiological fluids can be analyzed. offer baseline separation between methodology. Up to 3 columns in on your needs. series can be used for production virtually all 40 of the amino acids Please contact the Facility Manager If your demand is perfection in the and their derivatives which can of peptide analogues with yields of chromatography of protein/peptide concerning specific additional occur in physiological fluids. This approximately 0.2 mmole/column. hydrolysates you will be impressed We can synthesize up to 100 mg of requirements such as peptide with the excellent resolution the is ideal for complex samples such peptide for all types of applications. couplings or incorporation of as urine, plant extracts and fermen- modified amino acid residues. sulphonated polystyrene resin tation media. The purity of the cleaved and provides. This is an important