DOCSLIB.ORG

P Tift Dp Firtl

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
P Tift Dp Firtl REFERENCE ONLY UNIVERSITY OF LONDON THESIS Degree Year Name of Author p tift < 1 0 d P firtL COPYRIGHT This is a thesis accepted for a Higher Degree of the University of London. It is an unpublished typescript and the copyright is held by the author. All persons consulting the thesis must read and abide by the Copyright Declaration below. COPYRIGHT DECLARATION I recognise that the copyright of the above-described thesis rests with the author and that no quotation from it or information derived from it may be published without the prior written consent of the author. LOAN Theses may not be lent to individuals, but the University Library may lend a copy to approved libraries within the United Kingdom, for consultation solely on the premises of those libraries. Application should be made to: The Theses Section, University of London Library, Senate House, Malet Street, London WC1E 7HU. REPRODUCTION University of London theses may not be reproduced without explicit written permission from the University of London Library. Enquiries should be addressed to the Theses Section of the Library. Regulations concerning reproduction vary according to the date of acceptance of the thesis and are listed below as guidelines. A. Before 1962. Permission granted only upon the prior written consent of the author. (The University Library will provide addresses where possible). B. 1962 - 1974. In many cases the author has agreed to permit copying upon completion of a Copyright Declaration. C. 1975 - 1988. Most theses may be copied upon completion of a Copyright Declaration. D. 1989 onwards. Most theses may be copied. This thesis comes within category D. This copy has been deposited in the Library of I ( £ L—_____________ This copy has been deposited in the University of London Library, Senate □ House, Malet Street, London WC1E 7HU. C:\Documents and Settings\lproctor.ULL\Local Settings\Temporary Internet Files\OLK36\Copyright - thesis.doc MOLECULAR STUDY OF CELL CULTURE MODELS OF PARKINSON’S DISEASE AND HUNTINGTON’S DISEASE by Michael Orth, MD Department of Clinical Neurosciences Royal Free and University College Medical School University of London A thesis submitted, in fulfilment, for the degree of Doctor of Philosophy September 2004 1 UMI Number: U593085 All rights reserved INFORMATION TO ALL USERS The quality of this reproduction is dependent upon the quality of the copy submitted. In the unlikely event that the author did not send a complete manuscript and there are missing pages, these will be noted. Also, if material had to be removed, a note will indicate the deletion. Dissertation Publishing UMI U593085 Published by ProQuest LLC 2013. Copyright in the Dissertation held by the Author. Microform Edition © ProQuest LLC. All rights reserved. This work is protected against unauthorized copying under Title 17, United States Code. ProQuest LLC 789 East Eisenhower Parkway P.O. Box 1346 Ann Arbor, Ml 48106-1346 Abstract The discovery of the genetic basis of neurodegenerative disorders has enabled the generation of models to study their pathogenesis. In part one, human embryonic kidney cells with inducible expression of wild-type or mutant G209A a-synuclein modelled increased a-synuclein expression and a familial form of Parkinson’s disease, respectively. Both wild-type and mutant a- synuclein were localised to vesicles, some of which were catecholaminergic. Over­ expression of wild-type or mutant (G209A) a-synuclein alone did not reduce cell viability, cause oxidative stress or impair mitochondrial function. However, mutant a- synuclein expression enhanced the susceptibility to dopamine toxicity causing increased oxidative stress and cell death. This effect was similar to that of reserpine, an inhibitor of vesicular monoamine uptake, in controls. These results suggest that a-synuclein may play a role in dopamine compartmentalisation. Loss of function conferred by the G209A mutation could therefore increase cytoplasmic dopamine concentrations with subsequent cell damage or death. In part two, myoblast cell lines were established, and characterised, from the R6/2 mouse model of Huntington’s disease (HD). Mutant N-terminal huntingtin transgene over-expression was associated with significantly greater numbers of myotubes suggesting a role of huntingtin in muscle differentiation. In long-term culture, differentiated R6/2 myotubes, but not controls, formed nuclear huntingtin inclusions. Inclusion number depended upon culture medium conditions suggesting that environmental factors might be relevant. This model of HD in non-neuronal post­ mitotic cells may be useful to study the pathophysiology of, and possibly the effect of therapeutics on, huntingtin aggregate formation. The third part examined the suggestion that codon 129 homozygosity of the prion protein (PrP) gene may predispose to sporadic inclusion body myositis (sIBM). 2 Codon 129 zygosity in 41 sIBM muscle biopsies was not significantly different to results published in population studies in several Western countries suggesting sIBM is not linked to homozygosity at codon 129 of the PrP gene. 3 Declaration All of the experimental work in this thesis was performed by the author and the majority of this thesis is published (see Publications). 4 Acknowledgements There are many people who I am indebted to in one way or another. These include my supervisors, Tony Schapira and Mark Cooper, who have inspired and encouraged me each in their own way. I am grateful to Tony particularly for helping me to keep an eye on the “bigger picture” beyond the work necessary for this degree and for his ongoing support. To Mark I am grateful for teaching me patiently about scientific thinking and critical appraisal of my own work. Many insights came only after years on this educational path on which he led with great spirit and always in good humour. This work would have been very difficult without the help of Sarah Tabrizi. Not only did she welcome me with a warm heart but also she had laid some of the foundations for some of this work. And let’s not forget the encouraging “tannoy- DAAARLINGS” that brightened up the day! A special thanks has to go to Jan-Willem Taanman for always being there to answer questions and give advice, without him I would have struggled more! And then there were the “fellow strugglers” competing in the number of tissue culture plates and incubator space needed such as Paul Hart and Sion Williams, and later Pras and Chris. Thanks also to Ross King, Michelle and Jane W for help and their companionship, and to the secretary team who are always there to help and always have a friendly word. Last but not least I want to thank my family and friends, some far away “in Europe”, for their encouragement, and most of all Maria for her love and support even though this “thing” was taking up a sheer endless amount of time and energy. Without her I would most certainly have become even more of a recluse towards the end. Moltes gracies! 5 Dedication To my family; my parents, Hans and Heidi, and my sister, Meike Index page Abstract 2 Declaration 4 Acknowledgements 5 Dedication 6 Table of contents 7 List of Tables 16 List of Figures 17 Abbreviations 20 CHAPTER 1. General introduction 1.1. Introduction 23 1.1.1. Mitochondrial function 24 1.1.2. Types of cell death 26 1.1.2.1. Necrosis 26 1.1.2.2. Programmed cell death 27 1.1.3. Free radicals and cellular defence systems 31 1.1.4. Cellular consequences of oxidative stress 33 1.1.5. Excitotoxicity 35 1.1.6. The ubiquitin-proteasome system (UPS) 36 1.1.7. Models of neurodegenerative diseases 37 1.1.7.1. Toxin models 38 1.1.7.2. Knock-out models 38 1.1.7.3. Transgenic animal models 39 1.1.7.4. Cell culture models 39 7 CHAPTER 2. Material and Methods 2.1. Materials 42 2.2. Cell lines and cell culture 44 2.2.1. EcR 293 cell line (Invitrogen, UK) 44 2.2.2. NT2 cells (Ntera2/D1 )(Stratagene, UK) 44 2.2.3. SH-SY5Y neuroblastoma cells 45 2.2.4. R6/2 transgenic mice and myoblast cell lines 45 2.2.5. Myotube desmin stain and myotube counts 46 2.3. Cell culture 47 2.3.1. Cell freezing and defrosting 47 2.4. Molecular biology 48 2.4.1. The Ecdysone Mammalian Expression System 48 2.4.2. Generation of vectors 49 2.4.3. Transfection of cDNA 50 2.4.4. Ring cloning 51 2.4.5. DNA extraction from cells 52 2.4.6. DNA extraction from muscle tissue 51 2.4.7. Estimation of DNA concentration and purity 53 2.4.8. Polymerase chain reaction (PCR) 53 2.4.8.1. Amplification of cDNA in pIND constructs 54 2.4.8.2. Prion protein gene ( PRNP) PCR 54 2.4.9. PCR clean-up and sequencing 55 2.4.10. DNA digestion by restriction endonucleases 55 2.4.11. Detection of DNA 55 2.5.1. Cytochemical staining 56 2.5.1.1. Haematoxylin and Eosin 56 2.5.1.2. Modified Gomori trichrome 56 8 2.5.1.3. Modified Congo red 57 2.5.2. Immunocytochemistry 57 2.5.3. Immunohistochemistry 58 2.5.4. Antibodies 59 2.5.5. Catecholamine stain 59 2.5.6. Image analysis and photography 60 2.6. Protein assays 60 2.6.1. Protein extraction 60 2.6.2. Protein determination 61 2.6.2.1. The bicinchoninic acid copper assay (BCA) 61 2.6.2.2. The BioRad protein determination kit 61 2.6.3. Protein separation 62 2.6.4. Staining of protein gels 62 2.6.5. Immunostaining of blots 62 2.7. Enzyme analysis 63 2.7.1. Preparation of mitochondrial enriched fractions (MEFs) 64 2.7.2.
Load more

Recommended publications
  • Female Fellows of the Royal Society
    Female Fellows of the Royal Society Professor Jan Anderson FRS [1996] Professor Ruth Lynden-Bell FRS [2006] Professor Judith Armitage FRS [2013] Dr Mary Lyon FRS [1973] Professor Frances Ashcroft FMedSci FRS [1999] Professor Georgina Mace CBE FRS [2002] Professor Gillian Bates FMedSci FRS [2007] Professor Trudy Mackay FRS [2006] Professor Jean Beggs CBE FRS [1998] Professor Enid MacRobbie FRS [1991] Dame Jocelyn Bell Burnell DBE FRS [2003] Dr Philippa Marrack FMedSci FRS [1997] Dame Valerie Beral DBE FMedSci FRS [2006] Professor Dusa McDuff FRS [1994] Dr Mariann Bienz FMedSci FRS [2003] Professor Angela McLean FRS [2009] Professor Elizabeth Blackburn AC FRS [1992] Professor Anne Mills FMedSci FRS [2013] Professor Andrea Brand FMedSci FRS [2010] Professor Brenda Milner CC FRS [1979] Professor Eleanor Burbidge FRS [1964] Dr Anne O'Garra FMedSci FRS [2008] Professor Eleanor Campbell FRS [2010] Dame Bridget Ogilvie AC DBE FMedSci FRS [2003] Professor Doreen Cantrell FMedSci FRS [2011] Baroness Onora O'Neill * CBE FBA FMedSci FRS [2007] Professor Lorna Casselton CBE FRS [1999] Dame Linda Partridge DBE FMedSci FRS [1996] Professor Deborah Charlesworth FRS [2005] Dr Barbara Pearse FRS [1988] Professor Jennifer Clack FRS [2009] Professor Fiona Powrie FRS [2011] Professor Nicola Clayton FRS [2010] Professor Susan Rees FRS [2002] Professor Suzanne Cory AC FRS [1992] Professor Daniela Rhodes FRS [2007] Dame Kay Davies DBE FMedSci FRS [2003] Professor Elizabeth Robertson FRS [2003] Professor Caroline Dean OBE FRS [2004] Dame Carol Robinson DBE FMedSci
    [Show full text]
  • Smutty Alchemy
    University of Calgary PRISM: University of Calgary's Digital Repository Graduate Studies The Vault: Electronic Theses and Dissertations 2021-01-18 Smutty Alchemy Smith, Mallory E. Land Smith, M. E. L. (2021). Smutty Alchemy (Unpublished doctoral thesis). University of Calgary, Calgary, AB. http://hdl.handle.net/1880/113019 doctoral thesis University of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission. Downloaded from PRISM: https://prism.ucalgary.ca UNIVERSITY OF CALGARY Smutty Alchemy by Mallory E. Land Smith A THESIS SUBMITTED TO THE FACULTY OF GRADUATE STUDIES IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY GRADUATE PROGRAM IN ENGLISH CALGARY, ALBERTA JANUARY, 2021 © Mallory E. Land Smith 2021 MELS ii Abstract Sina Queyras, in the essay “Lyric Conceptualism: A Manifesto in Progress,” describes the Lyric Conceptualist as a poet capable of recognizing the effects of disparate movements and employing a variety of lyric, conceptual, and language poetry techniques to continue to innovate in poetry without dismissing the work of other schools of poetic thought. Queyras sees the lyric conceptualist as an artistic curator who collects, modifies, selects, synthesizes, and adapts, to create verse that is both conceptual and accessible, using relevant materials and techniques from the past and present. This dissertation responds to Queyras’s idea with a collection of original poems in the lyric conceptualist mode, supported by a critical exegesis of that work.
    [Show full text]
  • BIOLOGY 639 SCIENCE ONLINE the Unexpected Brains Behind Blood Vessel Growth 641 THIS WEEK in SCIENCE 668 U.K
    4 February 2005 Vol. 307 No. 5710 Pages 629–796 $10 07%.'+%#%+& 2416'+0(70%6+10 37#06+6#6+8' 51(69#4' #/2.+(+%#6+10 %'..$+1.1); %.10+0) /+%41#44#;5 #0#.;5+5 #0#.;5+5 2%4 51.76+105 Finish first with a superior species. 50% faster real-time results with FullVelocity™ QPCR Kits! Our FullVelocity™ master mixes use a novel enzyme species to deliver Superior Performance vs. Taq -Based Reagents FullVelocity™ Taq -Based real-time results faster than conventional reagents. With a simple change Reagent Kits Reagent Kits Enzyme species High-speed Thermus to the thermal profile on your existing real-time PCR system, the archaeal Fast time to results FullVelocity technology provides you high-speed amplification without Enzyme thermostability dUTP incorporation requiring any special equipment or re-optimization. SYBR® Green tolerance Price per reaction $$$ • Fast, economical • Efficient, specific and • Probe and SYBR® results sensitive Green chemistries Need More Information? Give Us A Call: Ask Us About These Great Products: Stratagene USA and Canada Stratagene Europe FullVelocity™ QPCR Master Mix* 600561 Order: (800) 424-5444 x3 Order: 00800-7000-7000 FullVelocity™ QRT-PCR Master Mix* 600562 Technical Services: (800) 894-1304 Technical Services: 00800-7400-7400 FullVelocity™ SYBR® Green QPCR Master Mix 600581 FullVelocity™ SYBR® Green QRT-PCR Master Mix 600582 Stratagene Japan K.K. *U.S. Patent Nos. 6,528,254, 6,548,250, and patents pending. Order: 03-5159-2060 Purchase of these products is accompanied by a license to use them in the Polymerase Chain Reaction (PCR) Technical Services: 03-5159-2070 process in conjunction with a thermal cycler whose use in the automated performance of the PCR process is YYYUVTCVCIGPGEQO covered by the up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., an authorized thermal cycler.
    [Show full text]
  • Acknowledgment of Reviewers, 2009
    Proceedings of the National Academy ofPNAS Sciences of the United States of America www.pnas.org Acknowledgment of Reviewers, 2009 The PNAS editors would like to thank all the individuals who dedicated their considerable time and expertise to the journal by serving as reviewers in 2009. Their generous contribution is deeply appreciated. A R. Alison Adcock Schahram Akbarian Paul Allen Lauren Ancel Meyers Duur Aanen Lia Addadi Brian Akerley Phillip Allen Robin Anders Lucien Aarden John Adelman Joshua Akey Fred Allendorf Jens Andersen Ruben Abagayan Zach Adelman Anna Akhmanova Robert Aller Olaf Andersen Alejandro Aballay Sarah Ades Eduard Akhunov Thorsten Allers Richard Andersen Cory Abate-Shen Stuart B. Adler Huda Akil Stefano Allesina Robert Andersen Abul Abbas Ralph Adolphs Shizuo Akira Richard Alley Adam Anderson Jonathan Abbatt Markus Aebi Gustav Akk Mark Alliegro Daniel Anderson Patrick Abbot Ueli Aebi Mikael Akke David Allison David Anderson Geoffrey Abbott Peter Aerts Armen Akopian Jeremy Allison Deborah Anderson L. Abbott Markus Affolter David Alais John Allman Gary Anderson Larry Abbott Pavel Afonine Eric Alani Laura Almasy James Anderson Akio Abe Jeffrey Agar Balbino Alarcon Osborne Almeida John Anderson Stephen Abedon Bharat Aggarwal McEwan Alastair Grac¸a Almeida-Porada Kathryn Anderson Steffen Abel John Aggleton Mikko Alava Genevieve Almouzni Mark Anderson Eugene Agichtein Christopher Albanese Emad Alnemri Richard Anderson Ted Abel Xabier Agirrezabala Birgit Alber Costica Aloman Robert P. Anderson Asa Abeliovich Ariel Agmon Tom Alber Jose´ Alonso Timothy Anderson Birgit Abler Noe¨l Agne`s Mark Albers Carlos Alonso-Alvarez Inger Andersson Robert Abraham Vladimir Agranovich Matthew Albert Suzanne Alonzo Tommy Andersson Wickliffe Abraham Anurag Agrawal Kurt Albertine Carlos Alos-Ferrer Masami Ando Charles Abrams Arun Agrawal Susan Alberts Seth Alper Tadashi Andoh Peter Abrams Rajendra Agrawal Adriana Albini Margaret Altemus Jose Andrade, Jr.
    [Show full text]
  • Annual Fellows' Meeting
    2020 Annual Fellows’ Meeting 3 December 2020 Annual Fellows’ Meeting: 3 December 2020 14.30 Annual General Meeting 14.50 Review of 2020 A series of short talks will highlight key activities this year. • Professor Sir Robert Lechler, President • Simon Denegri, Executive Director ne • Dr Rachel Quinn, Director of Medical Science Policy and Professor Guy Thwaites FMedSci, Professor of Infectious Diseases & Director of Oxford University Clinical Research Unit (OUCRU), Vietnam. Session o Session • Dr Virginia Newcombe, AMS Clinician Scientist Fellow; Consultant in Critical Care and Emergency Medicine, and Royal College of Emergency Medicine Associate Professor. • Nick Hillier, Director Of Communications and Mandy Rudczenko, Patient and carer reference group co-Chair, AMS winter project • Professor Paul Stewart FMedSci, Vice President (Clinical) 16.00 Break Fellows are invited to stay online during the break between sessions. Breakout spaces will allow Fellows to connect informally and move about freely to catch-up with colleagues and friends. 16:30 Handover to next Academy President • Sir Patrick Vallance: Reflections on Sir Robert Lechler’s Presidency • Professor Sir Robert Lechler • Professor Dame Anne Johnson wo 17.00 The Jean Shanks Lecture st Session t Session Palliative care for the 21 century Professor Irene Higginson OBE FMedSci Professor of Palliative Care and Policy and Director, Cicely Saunders Institute, King’s College London 18:00 End Agenda for the 2020 Annual General Meeting Academy of Medical Sciences Incorporated by Royal Charter RC000905 1. President’s welcome 2. Minutes Resolution 1 Approval of the minutes of the Annual General Meeting on 3 December 2019 3. Honorary Fellows Resolution 2 Proposal to elect as Honorary Fellows of the Academy • Sir Bruce Keogh • Professor Dame Ottoline Leyser • Professor Lucio Luzzatto 4.
    [Show full text]
  • Feamle Fellows 2015
    Female Fellows of the Royal Society Fellowship Professor Jan M Anderson FRS [1996] Dame Julia Higgins DBE FREng FRS [1995] Professor Judith Armitage FRS [2013] Professor Brigid Hogan FRS [2001] Professor Frances Ashcroft FMedSci FRS [1999] Professor Christine Holt FMedSci FRS [2009] Professor Gillian Bates FMedSci FRS [2007] Professor Judith Howard CBE FRS [2002] Professor Jean Beggs CBE FRS [1998] Professor Patricia Jacobs OBE FMedSci FRS [1993] Dame Jocelyn Bell Burnell DBE FRS [2003] Professor Lisa Jardine CBE FRS [2015]* Dame Valerie Beral DBE FMedSci FRS [2006] Dame Carole Jordan DBE FRS [1990] Dr Mariann Bienz FMedSci FRS [2003] Professor Victoria Kaspi FRS [2010] Professor Dorothy Bishop FBA FMedSci FRS [2014] Dr Olga Kennard OBE FRS [1987] Professor Elizabeth Blackburn AC FRS [1992] Professor Frances Kirwan DBE FRS [2001] Professor Andrea Brand FMedSci FRS [2010] Professor Jane Langdale FRS [2015] Professor Eleanor Burbidge FRS [1964] Professor Ottoline Leyser CBE FRS [2007] Professor Eleanor Campbell FRS [2010] Professor Ruth Lynden-Bell FRS [2006] Professor Doreen Cantrell CBE FMedSci FRS [2011] Professor Georgina Mace CBE FRS [2002] Professor Deborah Charlesworth FRS [2005] Professor Trudy Mackay FRS [2006] Professor Jennifer Clack FRS [2009] Professor Enid MacRobbie FRS [1991] Professor Jane Clarke FMedSci FRS [2015] Dr Philippa Marrack FMedSci FRS [1997] Professor Nicola Clayton FRS [2010] Professor Dusa McDuff FRS [1994] Professor Suzanne Cory AC FRS [1992] Professor Angela McLean FRS [2009] Professor Anne Cutler FRS [2015]
    [Show full text]
  • British Neuroscience Association
    Table of contents British Neuroscience Association ............................................................................................................ 2 The Idea for BNA2013: Festival of Neuroscience.................................................................................... 4 Message from the BNA President ........................................................................................................... 5 Message from the Programme Committee Co-Chair ............................................................................. 6 Partner Listing ......................................................................................................................................... 7 Festival Sponsor Listing ......................................................................................................................... 12 General Information ............................................................................................................................. 19 Programme at a Glance ........................................................................................................................ 23 SUNDAY 7th APRIL 2013 ..................................................................................................................... 23 MONDAY 8TH APRIL 2013 .................................................................................................................. 25 TUESDAY 9th APRIL 2013 ..................................................................................................................
    [Show full text]
  • Acknowledgment of Reviewers, 2014
    Acknowledgment of Reviewers, 2014 The PNAS editors would like to thank all the individuals who dedicated their considerable time and expertise to the journal by serving as reviewers in 2014. Their generous contribution is deeply appreciated. A Joseph Adams Seungkirl Ahn Hashim Al-Hashimi Luis Amaral Kjersti Aagard-Tillery Michael Adams Tero Ahola Javey Ali Rommie Amaro Duur Aanen Paul Adams Cyrus Aidun Antonios Aliprantis Bruno Amati Jorge Abad Ralf Adams Iannis Aifantis Kari Alitalo Jayakrishna Ambati Alejandro Aballay Lee Adamson Kazuyuki Aihara Rob Alkemade Richard Ambinder Adam Abate John Adelman Judd Aiken Michael Alkire Stanley Ambrose Alireza Abbaspourrad Karen Adelman Elizabeth Ainsworth Robin Allaby Indu Ambudkar Jonathan Abbatt Zach Adelman William Aird Milan P. Allan Chris Amemiya Patrick Abbot Pia Ädelroth Edoardo Airoldi Marc Allard Jan Amend Albert Abbott Sarah Ades Joanna Aizenberg Hunt Allcott Amal Amer Allison Abbott Ilensami Adesida Michael Aizenberg Martin Allday Stefan Ameres Derek Abbott Becky Adkins Myles Akabas Andrew Allen Sebastian Amigorena Larry Abbott Elizabeth Adkins-Regan Ilke Akartuna Charles Allen Mohammed Amin Nicholas Abbott Andy Adler Erol Akcay Dale Allen Ido Amit Paul Abbyad Frederick Adler Mark Akeson David Allen Gabriel Amitai Omar Abdel-Wahab Gregory Adler Christopher Akey Eric Allen Sygal Amitay Yalchin Abdullaev Lynn Adler Ethan Akin Irving Allen Markus Ammann Ikuro Abe Roee Admon Shizuo Akira James Allen David Amodio Jun-ichi Abe Ralph Adolphs Gustav Akk John Allen Angelika Amon Koji Abe Jose Adrio Mikael Akke Karen Allen Christopher Amos Goncalo Abecasis Radoslav Adzic Serap Aksoy Melinda Allen Linda Amos Stephen Abedon Markus Aebi Anastasia Aksyuk Nicola Allen Derk Amsen Markus Abel Toni Aebischer Klaus Aktories Paul Allen Ronald Amundson Moshe Abeles G.
    [Show full text]
  • EMBO & EMBC Annual Report 2002
    EMBO & EMBC annual report 2002 European Molecular Biology Organization Meyerhofstrasse 1 69117 Heidelberg Germany phone: ++49 6221 8891 0 fax: ++49 6221 8891 200 e-mail: [email protected] www.embo.org table of contents Introduction preface by Frank Gannon . .4 preface by Herbert Jäckle . .5 preface by Julio Celis . .6 EMBO & EMBC present & past aims of EMBO, EMBC & EMBL . .10 timeline & brief history . .12 EMBO & EMBC actions 2002 . .14 joint EMBO & EMBC activities fellowship programme . .20 fellows network . .22 courses & workshops programme . .24 young investigator programme . .26 electronic information programme . .28 EMBO additional activities the EMBO journal . .32 EMBO reports . .34 science & society programme . .36 restart programme . .38 EMBO gold medal . .40 EMBO communication award . .41 EMBO lectures . .42 EMBO sectorial meetings . .43 EMBO world programme . .44 press & public relations . .46 appendix delegates & advisers from the EMBC member states . .50 EMBO council members 2002 . .54 2 EMBO committees 2002 . .55 EMBO council members 2003 . .56 EMBO committees 2003 . .57 new EMBO members 2002 . .58 advisory editorial board for The EMBO Journal & EMBO reports . .66 long-term fellowship awards 2002 . .68 statistics on long-term fellowship awards . .80 geographical distribution for long-term fellowships 2002 . .82 short-term fellowship awards 2002 . .84 statistics on short-term fellowship awards . .96 geographical distribution for short-term fellowships 2002 . .98 EMBO young investigators 2002 . .100 EMBO/HHMI scientists 2002 & EMBO young investigators 2000 & 2001 . .101 statistics on EMBO young investigators . .102 participation of women in EMBO activities . .104 practical & lecture courses 2002 . .106 workshops 2002 . .108 world programme activities 2002 . .110 press releases & press briefs 2002 .
    [Show full text]
  • Hereditary Disease Foundation H Re Edit Yra F H Nu I of Id Reh Adn Seai Edit Noita Ase Yrta N Y F Nuo and Noita N
    Scie nti fic Advis ory Board HHereditaryeredittary DDiseaseiseaase FFoundationoundation SCIENTIFIC ADVISORY BOARD “I met patients with HD when I was doing my Ph.D. and was incredibly moved. My passion to find an effective treatment for Huntington’s disease drives everything I do. From the beginning I have understood the importance of the Hereditary Disease Foundation and its critical role in advancing research towards therapies for HD.” Sarah J. Tabrizi, FRCP, Ph.D., FMedSci. University College London Institute of Neurology Scientific Advisory Board Hereditary Disease Foundation SCIENTIFIC ADVISORY BOARD The Hereditary Disease Foundation’s Scientific Advisory Board is composed of distinguished scientists from around the world. The Board sets the scientific priorities for the Foundation, reviews grant and fellowship applications and selects the most innovative and promising research projects for funding. Anne B. Young, M.D., Ph.D. — Chair John Mazziotta, M.D., Ph.D. Massachusetts General Hospital UCLA Health Sciences Harvard Medical School Diane E. Merry, Ph.D. Leslie M. Thompson, Ph.D. — Vice Chair Thomas Jefferson University University of California, Irvine Richard I. Morimoto, Ph.D. Gillian P. Bates, FMedSci., Ph.D., FRS Northwestern University UCL Institute of Neurology A. Jennifer Morton, Ph.D., Sc.D., FRSB C. Frank Bennett, Ph.D. University of Cambridge Ionis Pharmaceuticals Richard C. Mulligan, Ph.D. Yvette Bordelon, M.D., Ph.D. Harvard Medical School David Geffen School of Medicine at UCLA Harry T. Orr, Ph.D. Alan Buckler, Ph.D. University of Minnesota Scholar Rock, Inc. Henry L. Paulson, M.D., Ph.D. Marie-Françoise Chesselet, M.D., Ph.D. University of Michigan David Geffen School of Medicine at UCLA Christopher E.
    [Show full text]
  • Trustees' Report and Financial Statements
    TRUSTEES’ REPORT AND FINANCIAL STATEMENTS 1 Trustees’ report and financial statements For the year ended 31 March 2014 2 TRUSTEES’ REPORT AND FINANCIAL STATEMENTS Trustees Executive Director The Trustees of the Society are the Dr Julie Maxton members of its Council, who are elected Statutory Auditor by and from the Fellowship. Council is Deloitte LLP chaired by the President of the Society. Abbots House During 2013/14, the members of Council Abbey Street were as follows: Reading President RG1 3BD Sir Paul Nurse Bankers Treasurer The Royal Bank of Scotland Professor Anthony Cheetham 1 Princess Street London Biological Secretary EC2R 8BP Sir John Skehel** Dame Jean Thomas DBE* Investment Managers Rathbone Brothers PLC Physical Secretary 1 Curzon Street Sir John Pethica London Foreign Secretary W1J 5FB Professor Martyn Poliakoff CBE Internal Auditors PricewaterhouseCoopers LLP Members of Council Cornwall Court Professor Gillian Bates * 19 Cornwall Street Sir John Beddington CMG Birmingham Professor Geoffrey Boulton OBE B3 2DT Professor Andrea Brand ** Dr Simon Campbell * Professor Michael Cates ** Professor John Collinge * Registered Charity Number 207043 Dame Athene Donald DBE Professor Carlos Frenk Registered address Dame Wendy Hall DBE ** 6 – 9 Carlton House Terrace Professor Alexander Halliday * London SW1Y 5AG Professor Judith Howard * Dame Frances Kirwan DBE royalsociety.org Professor Ottoline Leyser CBE Dr Robin Lovell-Badge * Professor Angela McLean ** Professor Georgina Mace CBE ** Professor Roger Owen Professor Timothy Pedley Dame Nancy Rothwell DBE ** Professor Nicholas Tonks * Professor John Wood * Up to 29 November 2013 ** From 29 November 2013 TRUSTEES’ REPORT AND FINANCIAL STATEMENTS 3 Contents President’s foreword ................................................................................................. 4 Executive Director’s report ........................................................................................
    [Show full text]
  • Trustees' Report and Financial Statements
    Trustees’ report and financial statements For the year ended 31 March 2012 Trustees Executive Director Statutory Auditor The Trustees of the Society are the Dr Julie Maxton PKF (UK) LLP members of its Council, who are Farringdon Place Registered Address elected by the Fellowship. As laid 20 Farringdon Road 6 – 9 Carlton House Terrace down in the Society’s Royal Charter, London EC1M 3AP London SW1Y 5AG 10 of the 21 members of Council retire each year. Council is chaired by royalsociety.org the President of the Society. During Bankers Registered charity No 207043 2011/12, the members of Council were Natwest as follows: 1 Princess Street London EC2R 8BP President Sir Paul Nurse Foreign Secretary Investment Managers Professor Lorna Casselton* Rathbone Brothers PLC Professor Martyn Poliakoff** 1 Curzon Street London W1J 5FB Physical Secretary Professor John Pethica Biological Secretary Internal Auditors Dame Jean Thomas PricewaterhouseCoopers LLP Cornwall Court Treasurer 19 Cornwall Street Sir Peter Williams Birmingham B3 2DT Members of Council Professor David Barford* Professor Gillian Bates** Professor Andrew Blake Dr Simon Campbell** Professor Richard Catlow* Professor Anthony Cheetham Professor John Collinge** Professor Peter Donnelly Dr Matthew Freeman* Professor Brian Greenwood* Professor Alexander Halliday** Professor Andrew Hopper* Professor Judith Howard** Dame Louise Johnson* Dr Robin Lovell-Badge** Professor John McWhirter Professor Kim Nasmyth** Dame Linda Partridge Professor John Pyle* Professor Trevor Robbins Professor Wilson Sibbett Sir Christopher Snowden** Professor Nicholas Tonks** Professor Veronica van Heyningen * Sir Alan Wilson* * Member until 30 November 2011 ** Member since 30 November 2011 Front cover image: plan of the water- powered engine for the fifth arch of Old London Bridge, by John Smeaton, 1768.
    [Show full text]