Polyclonal Antibody to Carabin (N-Term) - Purified

OriGene Technologies, Inc. OriGene Technologies GmbH

9620 Medical Center Drive, Ste 200 Schillerstr. 5 Rockville, MD 20850 32052 Herford UNITED STATES GERMANY Phone: +1-888-267-4436 Phone: +49-5221-34606-0 Fax: +1-301-340-8606 Fax: +49-5221-34606-11 [email protected] [email protected]

AP05728PU-N Polyclonal Antibody to Carabin (N-term) - Purified

Alternate names: TBC1 domain family member 10C, TBC1D10C Quantity: 0.1 mg Concentration: 1.0 mg/ml Background: Carabin differs from other members of the TBC1 domain protein family, which appear to play a role in the regulation of cell growth and differentiation. Studies demonstrate that Carabin is part of a negative regulatory loop for the intracellular TCR signalling pathway and acts as an inhibitor of the Ras signalling pathway. Carabin is also believed to mediate crosstalk between calcineurin and Ras. Carabin is most abundantly expressed in spleen and peripheral blood leucocytes. Uniprot ID: Q8IV04 NCBI: NP_940919.1 GeneID: 374403 Host / Isotype: Rabbit / IgG Immunogen: A 17 amino acid peptide from near the amino terminus of human Carabin. Format: State: Liquid purified IgG Buffer System: Phosphate buffered saline containing 0.02% Sodium Azide (NaN3) Applications: Western blot: 1 - 2 µg/ml; detects a band of approximately 55kDa in human spleen tissue cell lysates. Immunohistochemistry on paraffin sections (requires antigen retrieval). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: This antibody recognises Carabin, also known as TBC1 domain family member 10C (TBC1D10C), a 50 kDa protein belonging to the TBC1 domain family of proteins. This antibody does not cross react with either TBC1D10A or TBC1D10B. Species: Human, Mouse. Other species not tested. Storage: Store the antibody undiluted at 2-8°C for up to one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. Caution: (A full Health and Safety assessment is available upon request) This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only. General Readings: 1. Pan F, Sun L, Kardian DB, Whartenby KA, Pardoll DM, Liu JO. Feedback inhibition of calcineurin and Ras by a dual inhibitory protein Carabin. Nature. 2007 Jan 25;445(7126):433-6. Epub 2007 Jan 17. PubMed PMID: 17230191. 2. Weil, R., et al. (2006) Deciphering the pathway from the TCR to NFkappaB. Cell Death Differ.; 13:826 - 833. 3. Im SH, Rao A. Activation and deactivation of gene expression by Ca2+/calcineurin-

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20130814 1 / 2 AP05728PU-N: Polyclonal Antibody to Carabin (N-term) - Purified

NFAT-mediated signaling. Mol Cells. 2004 Aug 31;18(1):1-9. PubMed PMID: 15359117. 4. White, R.A., et al. (2000) The gene encoding TBC1D1 with homology to the tre2/USP6 oncogene, Bub2, and cdc16 maps to mouse chromosome 5 and human chromosome 4. Cytogenet. Cell Genet.; 89:272 - 275 Pictures: Formalin fixed, paraffin embedded human spleen with Rabbit anti Human Carabin (AP05728PU-N) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0).

Western blot analysis of whole cell lysate from Daudi, Burkitt's lymphoma derived cells probed with Rabbit anti Human Carabin (AP05728PU-N) at 1(A) and 2(B) µg/ml

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20130814 2 / 2