bioRxiv preprint doi: https://doi.org/10.1101/2020.03.22.997809; this version posted March 25, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 1 Evaluation of biodiversity in estuaries using environmental DNA metabarcoding 2 (Short title: Biodiversity of five estuaries) 3 4 5 Hyojin Ahn1,2*, Manabu Kume1, Yuki Terashima1, Feng Ye1,3, Satoshi Kameyama3, Masaki 6 Miya4, Yoh Yamashita1, Akihide Kasai2 7 8 9 1Connectivity of Hills, Humans and Oceans Unit, Kyoto University, Oiwake, 10 Kitashirakawa, Sakyo, Kyoto, Japan 11 2Faculty of Fisheries Sciences, Hokkaido University, Minatocho, Hakodate, Hokkaido, 12 Japan 13 3Center for Environmental Biology and Ecosystem, National Institute for Environmental 14 Studies, Onogawa, Tsukuba, Ibaraki, Japan 15 4Department of Ecology and Environmental Sciences, National History Museum and 16 Institute, Aobacho, Chuo, Chiba, Japan 17 18 19 *Corresponding author 20 E-mail:
[email protected] 21 22 23 24 25 1 bioRxiv preprint doi: https://doi.org/10.1101/2020.03.22.997809; this version posted March 25, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 26 Funding 27 This study was conducted under the Link Again Program, part of Connectivity of Hills, 28 Humans and Oceans, funded by the Nippon Foundation, attained by YY. The funder had no 29 role in study design, data collection and analysis, decision to publish, or preparation of the 30 manuscript.