Wesley Alan Larson [email protected] || larsonlab.wordpress.com || @Wes_Larson1 || 715-346-3150

Current positions Assistant Unit Leader, U.S. Geological Survey Wisconsin Cooperative Fishery Research Unit, 2016-present Affiliate faculty, University of Wisconsin-Stevens Point, College of Natural Resources, 2016-present

Education Ph.D. in Fisheries: University of Washington School of Aquatic & Fishery Sciences (UW SAFS), 2015 Dissertation title: Using genetic tools to inform management and study local adaptation in Pacific salmon Major advisor: Dr. Lisa Seeb B.S. in Marine Biology: University of California, Santa Cruz, 2009

Honors and Awards Genetics Section of the American Fisheries Society Early Career Award, 2018 W.F. Thompson Award for best student paper in fisheries science published in 2014, 2016 National Science Foundation Graduate Research Fellowship, 2012-2015 Faculty Merit Award, UW SAFS, 2013 Fellowship, UW SAFS H. Mason Keeler Endowment for Excellence, 2010-2011

Funded Research Grants Support for fisheries genetics research in Wisconsin. Funding agency: Wisconsin Department of Natural Resources. Duration: reoccurring. Total funding: approximately $150,000 per year. PI: W. Larson. Using genomics to delineate stock structure and create a standardized genetic resource for Great Lakes walleye. Funding Agency: Great Lakes Fishery Commission. Duration: 2019- 2020. Total funding: $195,671. PI: W. Larson. Using Genomics to improve stock structure resolution and assess recruitment dynamics of lake whitefish in Lake Michigan. Funding agency: Great Lakes Fishery Trust. Duration: 2019-2021. Total funding: $161,727. PI: W. Larson. Developing a high-throughput marker panel for lake sturgeon to standardize analyses across the Great Lakes. Funding Agency: Great Lakes Fish and Wildlife Restoration Act. Duration: 2019-2020. Total funding: $115,477. PI: W. Larson. Historical and Contemporary Genetic Diversity of Coregonus Species from Lake Michigan. Funding agency: Great Lakes Restoration Initiative. Duration: 2018-2020. Total funding: $134,160. PIs: W. Stott and W. Larson. Genetic assessment of Boardman River fish populations prior to dam removal. Funding agency: Great Lakes Restoration Initiative and Great Lakes Fishery Commission. Duration: 2018- 2020. Total funding: $150,000. PI: W. Larson. Using Genomic Tools to Investigate Adaptive Diversity in Great Lakes Cisco. Funding

W.A Larson 1

Agency: Great Lakes Fishery Trust. Duration: 2018-2019. Total funding: $105,000. PI: W. Larson. Development of a genetic linkage map for cisco Coregonus artedi to facilitate integrated studies of adaptive diversity. Funding Agency: Great Lakes Restoration Initiative. Duration: 2017-2019. Total funding: $179,299. PIs: W. Larson and W. Stott. Developing genomic resources for Great Lakes cisco to facilitate species identification and investigate adaptation. Funding Agency: Great Lakes Fish and Wildlife Restoration Act. Duration: 2017-2019. Total funding: $76,144. PI: W. Larson. Using genetics to inform conservation of Iowa brook trout. Funding Agency: Iowa Department of Natural Resources. Duration: 2017-2019. Total funding: $20,000. PI: W. Larson. Development of eDNA techniques to detect copperbelly watersnake. Funding Agency: U.S. Fish and Wildlife Service. Duration: 2017-2019. Total funding: $24,970. PI: W. Larson Using genetics to evaluate survival and natural reproduction of muskellunge in Wisconsin Lakes. Funding Agency: Hugh Becker Foundation. Duration 2018-2019. Total funding: $10,000. PI: W. Larson. Development of a Decision Support Tool (DST) for the use of environmental DNA. Funding agency: USGS Invasive Species Program. Duration: 2018-2020. Total funding: $218,802. PI: J. Amberg, co-PIs: W. Larson and J. Vander Zanden. Stock structure and contribution of west and east basin walleye to recreational and commercial fisheries in Lake Erie (co-PI). Funding Agency: Ohio Sea Grant. Duration: 2018-2010. Total funding: $119,724. PI: S. Ludsin, co-PI: W. Larson and six others. Spawning site contribution and movements of lake whitefish in northwestern Lake Michigan. Funding Agency: Great Lakes Fish and Wildlife Restoration Act. Duration: 2017-2021. Total funding: $276,962. PI: D. Isermann, co-PIs: W. Larson and seven others. Food web interactions among walleyes, lake whitefish, and yellow perch in Green Bay. Funding agency: Wisconsin Sea Grant. Duration: 2018-2020. Total funding: $197,007. PI: D. Isermann, co-PIs: W. Larson and twelve others. Genetic diversity among Great Lakes cisco species: exploring taxonomic and population boundaries. Funding agency: Great Lakes Restoration Initiative. Duration: 2019. Total funding: $73,658. PI: W. Stott, co-PIs: W. Larson and eight others. Building molecular tools for coregonine species identification. Funding agency: Great Lakes Restoration Initiative. Duration: 2019. Total funding: $43,632. PI: W. Stott, co-PIs: W. Larson and seven others. Transforming conservation genetics theory into practice: using adaptively important genes to conserve discrete populations of economically important fishes. Funding Agency: Hall Conservation Genetics Award, University of Washington. Duration: 2015. Total funding: $10,000. PI: W. Larson. Investigating the genetic basis of local adaptation in sockeye salmon from the Wood River Lakes. Funding Agency: UW SAFS H. Mason Keeler Endowment for Excellence. Duration: 2014-2015. Total funding: $21,441. PI: W. Larson.

Pending Research Grants Applying molecular techniques to assess the impacts of non-native predators on juvenile Chinook salmon migrating through the Sacramento-San Joaquin delta. Funding Agency: Delta Water Quality and Ecosystem Restoration Grant Program. Duration: 2020-2022. Funding

W.A Larson 2

requested: $1,489,561. PI: M. Henderson, co-PIs: W. Larson, S. Sethi, C. Michel, A. Kinziger, D. Pearse, and B. Singh. Combining genetics, otolith microchemistry, and vital rate estimation to inform restoration and management of fish populations in the UMRS. Funding agency: Upper Mississippi River Restoration Science in Support of Management Program. Duration: 2019-2021. Funding requested: $179,816. PI: W. Larson

Peer-reviewed publications Meek, M.H. and W.A. Larson (equal contributions). The future is now: Amplicon sequencing and sequence capture usher in the conservation genomics era. In press. Molecular Ecology Resources. Sethi, S.A., W.A. Larson, K.N. Turnquist, and D.A. Isermann. 2018. Estimating the number of contributors to DNA mixtures provides a novel tool for ecology. Methods in Ecology and Evolution. Online early. DOI: https://doi.org/10.1111/2041-210X.13079 Dembkowski, D.J., D.A. Isermann, S.R. Hogler, W.A. Larson, and K.N. Turnquist. 2018. Stock Structure, Dynamics, Demographics, and Movements of Walleyes Spawning in Four Tributaries to Green Bay. Journal of Great Lakes Research, 44(5) 907-978. DOI: https://doi.org/10.1016/j.jglr.2018.07.002 Defilippo, L.B., D.E. Schindler, J.L. Carter, T.E. Walsworth, T.J. Cline, W.A. Larson, T. Buehrens. 2018. Associations of stream geomorphic conditions and alternative reproductive tactics among sockeye salmon populations. Journal of Evolutionary Biology, 31(2) 239-253. DOI: 10.1111/jeb.13217 Larson, W.A., M.T. Limborg, G.J. McKinney, D.E. Schindler, J.E. Seeb, and L.W. Seeb. 2017. Genomic islands of divergence linked to ecotypic variation in sockeye salmon. Molecular Ecology, 26(2) 554-570. DOI: 10.1111/mec.13933 Larson, W.A., Y. Palti, G. Gao, K.T. Warheit, and J.E. Seeb. 2017. Rapid discovery of SNPs that differentiate hatchery steelhead trout from ESA-listed natural-origin steelhead trout using a 57K SNP array. Canadian Journal of Fisheries and Aquatic Sciences, 75(7) 1160-1168. DOI: https://doi.org/10.1139/cjfas-2017-0116 Turnquist, K.N., W.A. Larson, J.M. Farrell, P.A. Hanchin, K.L. Kapuscinski, L.M. Miller, K.T. Scribner, C.C. Wilson, B.L. Sloss. 2017. Genetic structure of muskellunge in the Great Lakes region and the effects of supplementation on genetic integrity of wild populations. Journal of Great Lakes Research, 43(6) 1141-1152. DOI: 10.1016/j.jglr.2017.09.005 Limborg, M.T, W.A. Larson, K. Shedd, L.W. Seeb, and J.E. Seeb. 2017. Novel RAD sequence data reveal a lack of genomic divergence between dietary ecotypes in a landlocked salmonid population. Conservation Genetics Resources 1-3. DOI: 10.1007/s12686-017-0791-4, IP- 084201 Limborg, M.T., W.A. Larson, L.W. Seeb, and J.E. Seeb. 2017. Screening of duplicated loci reveals hidden divergence patterns in a complex salmonid genome. Molecular Ecology, 26(17) 4509-4522. DOI: 10.1111/mec.14201, IP-083394 McKinney, G.J., W.A. Larson, L.W. Seeb, and J.E. Seeb. 2017. RADseq provides unprecedented insights into molecular ecology and evolutionary genetics: comment on Breaking RAD by Lowry et al. (2016). Molecular Ecology Resources, 17(3): 356-361. DOI: 10.1111/1755-0998.12649 Larson, W.A., P.J. Lisi, J.E. Seeb, L.W. Seeb, and D.E. Schindler. 2016. Major histocompatibility complex diversity is positively associated with stream water temperatures

W.A Larson 3

in proximate populations of sockeye salmon. Journal of Evolutionary Biology, 29(9): 1846- 59. DOI: 10.1111/jeb.12926 Waples, R.K, W.A. Larson, and R.S. Waples. 2016. Estimating contemporary effective population size in non-model species using linkage disequilibrium across thousands of loci. Heredity, 117(4) 233-40. DOI: 10.1038/hdy.2016.60 Larson, W.A., G.J. McKinney, J.E. Seeb, and L.W. Seeb. 2016. Identification and characterization of sex-associated loci in sockeye salmon using genotyping-by-sequencing and comparison with a sex-determining assay based on the sdY gene. Journal of Heredity, 107(6) 559-66. DOI: 10.1093/jhered/esw043 McKinney, G.J., L.W. Seeb, W.A. Larson, D. Gomez-Uchida, M.T. Limborg, M.S.O. Brieuc, M.V. Everett, K.A. Naish, R.W. Waples, and J.E. Seeb. 2016. An integrated linkage map reveals candidate genes underlying adaptive variation in Chinook salmon (Oncorhynchus tshawytscha). Molecular Ecology Resources, 16(3) 769-783. DOI: 10.1111/1755-0998.12479 Larson, W.A., J.E. Seeb, M.T. Limborg, G.J. McKinney, M.V. Everett, and L.W. Seeb. 2015. Identification of multiple QTL hotspots in sockeye salmon (Oncorhynchus nerka) using genotyping by sequencing and a dense linkage map. Journal of Heredity, 107(2) 122-133. DOI: 10.1093/jhered/esv099 Stockwell, B., W.A. Larson, R.K. Waples, R.A. Abesamis, L.W. Seeb, and K.E. Carpenter. 2015. The application of genomics to inform conservation of a functionally important reef fish (Scarus niger) in the Philippines. Conservation Genetics, 17(1) 239-249. DOI: 10.1007/s10592-015-0776-3 Candy, J.R, N.R., Campbell, M.H. Grinnell, T.D. Beacham, W.A. Larson, and S.R. Narum. 2015. Population differentiation determined from putative neutral and divergent adaptive genetic markers in Eulachon (Thaleichthys pacificus, Osmeridae), an anadromous Pacific smelt. Molecular Ecology Resources, 15(6) 1421-1434. DOI: 10.1111/1755-0998.12400 Larson, W.A., J.E. Seeb, T.H. Dann, D.E. Schindler, and L.W. Seeb. 2014. Signals of heterogeneous selection at an MHC locus in geographically proximate ecotypes of sockeye salmon. Molecular Ecology, 23(22) 5448-5461. DOI: 10.1111/mec.12949 Bond, M.H, P.A. Crane, W.A. Larson, T.P. Quinn. 2014. Is isolation by adaptation driving genetic divergence among proximate char populations? Ecology and Evolution 4(12) 2515- 2531. DOI: 10.1002/ece3.1113. Larson, W.A., J.E. Seeb, C.E. Pascal, W.D. Templin, and L.W. Seeb. 2014. SNPs identified through genotyping-by-sequencing improve genetic stock identification of Chinook salmon (Oncorhynchus tshawytscha) from western Alaska. Canadian Journal of Fisheries and Aquatic Sciences, 71(5): 698-708. DOI: 10.1139/cjfas-2013-0502 Larson, W.A., L.W. Seeb, M.V. Everett, R.K. Waples, W.D. Templin, and J.E. Seeb. 2014. Genotyping by sequencing resolves shallow population structure to inform conservation of Chinook salmon (Oncorhynchus tshawytscha). Evolutionary Applications, 7(3):355-369. DOI: 10.1111/eva.12128 Gruenthal, K.M., D.A. Witting, T. Ford, M.J. Neuman, J.P. Williams, D.J. Pondella, II, A. Bird, N. Caruso, J.R. Hyde, L.W. Seeb, and W.A. Larson. 2014. Development and application of genomic tools to the restoration of green abalone in southern California. Conservation Genetics, 15:109-121. DOI: 10.1007/s10592-013-0524-5 Larson, W.A., F.M. Utter, K.W. Myers, W.D. Templin, J.E. Seeb, C.M. Guthrie, A.V. Bugaev, and L.W. Seeb. 2013. Single-nucleotide polymorphisms reveal distribution and migration of Chinook salmon (Oncorhynchus tshawytscha) in the Bering Sea and North

W.A Larson 4

Pacific Ocean. Canadian Journal of Fisheries and Aquatic Sciences 70:128-141. DOI: 10.1139/cjfas-2012-0233

Publications in review or revision Ruzich, J, K. Turnquist, N. Nye, D. Rowe, and W. Larson. Isolation by a hydroelectric dam induces minimal impacts on genetic diversity and population structure in six fish species. In review. Conservation Genetics. Habicht, C., A. Barclay, H. Hoyt, K. Turnquist, and W. Larson. Discriminating among Pacific and Atlantic salmon species using 5´–3´ exonuclease (TaqMan) scatterplot distributions: Underlying variation provides basis for genotyping-by-sequencing solutions. In revision. Journal of Fish and Wildlife Management. Larson, W.A., T.H. Dann, M.T. Limborg, G.J. McKinney, J.E. Seeb, and L.W. Seeb. Parallel signatures of selection at genomic islands of divergence and the MHC in ecotypes of sockeye salmon across Alaska. In revision. Molecular Ecology.

Other media Gerbyshak, J., W.A. Larson, and K. Turnquist. 2017. Assessment of Leech Lake strain muskellunge stocking in Lake Wissota, WI. Extended abstract published in Muskellunge Management: Fifty Years of Cooperation Among Anglers, Scientists, and Fisheries Biologists. American Fisheries Society, Symposium 85, Bethesda, MD. Turnquist, K.N., W.A. Larson, J.M. Farrell, P.A. Hanchin, K.L. Kapuscinski, L.M. Miller, K.T. Scribner, C.C. Wilson, B.L. Sloss. 2017. Genetic structure of muskellunge in the Great Lakes region and the effects of supplementation on genetic integrity of wild populations. Extended abstract published in Muskellunge Management: Fifty Years of Cooperation Among Anglers, Scientists, and Fisheries Biologists. American Fisheries Society, Symposium 85, Bethesda, MD. Mitro, M., J. Griffin, W.A. Larson, and D. Rowe. 2017. Brook Trout Genetics: History and Future Management in Wisconsin. Wisconsin Trout Magazine, Spring 2017. Larson, W.A. 2014. AFS Genetics Section update: Parentage-based (genetic) tagging in the spotlight at the Western Division Annual Meeting. Fisheries, 39(2): 58. Boatright, C, W.A. Larson, L.W. Seeb, R. Hilborn. 2015. Estimating the Stock Composition in the Sockeye Salmon Fishery in the Outer Port Heiden and Ilnik Sections of Alaska’s North Peninsula Fishery, 2014-15. Report prepared for the Bristol Bay Science and Research Institute. Project results were covered by KDLG, Dillingham, AK in a news article and radio segment (http://kdlg.org/post/genetics-study-confirms-fish-oph-bound-bristol-bay#stream/0). Larson, W.A. 2014. AFS Genetics Section update: Parentage-based (genetic) tagging in the spotlight at the Western Division Annual Meeting. Fisheries, 39(2): 58.

Selected presentations Larson, W.A., M. Limborg, G. McKinney, T. Dann, J. Seeb, and L. Seeb. 2018. Understanding the genetic basis of ecotypic variation in sockeye salmon from across Alaska. Midwest Population Genetics Meeting. Larson, W.A., R.K. Waples, and R.S. Waples. Estimating effective size with genomic data: benefits and pitfalls. Annual meeting of the Canadian Society for Ecology and Evolution. Larson, W.A., M. Limborg, G. McKinney, T. Dann, J. Seeb, and L. Seeb. 2018.

W.A Larson 5

Understanding the genetic basis of ecotypic variation in sockeye salmon from across Alaska. Annual meeting of the Western Division of the American Fisheries Society. Larson, W.A., K.N. Turnquist, B. Erdman, M. Mitro, and J. Griffin. 2018. Using genetic tools to inform conservation of Wisconsin’s native brook trout. 78th Midwest Fish and Wildlife Conference. Milwaukee, WI. Larson, W.A., K.N. Turnquist, C.R. Bronte, S.D. Hanson, T.J. Treska, M.S. Kornis, B.L. Sloss, and W. Stott. 2017. Great Lakes Fisheries Commission Lakes Committee Meeting, Ypsilanti, MI. Larson, W.A. 2017. The importance of preserving adaptive genetic diversity in Great Lakes coregonids. Lake Michigan Technical Committee meeting, Zion, IL. Larson, W.A., K. Turnquist, J. VanDeHey, B. Blackwell, S. Chipps, B. Sloss, and A. Rosburg. 2016. Genomic signatures of selection in yellow perch populations with variable growth trajectories and mortality rates. Annual Meeting of the American Fisheries Society. Kansas City, Missouri. Larson, W.A. 2016. The utility of genomics for fisheries management: lessons from Pacific salmon. Wisconsin Chapter of the American Fisheries Society. La Crosse, Wisconsin. Larson, W.A., G.J. McKinney, M.T. Limborg, J.E. Seeb, and L.W. Seeb. 2015. Genomic islands of divergence show strong signatures of selection among three ecotypes of sockeye salmon. Annual meeting of the American Fisheries Society. Portland, OR. Larson, W.A., J.E. Seeb, T.H. Dann, and L.W. Seeb. 2014. Signals of heterogeneous selection at an MHC locus in geographically proximate ecotypes of sockeye salmon. Western Society of Naturalists Annual Meeting. Larson, W.A., J.E. Seeb, T.H. Dann, and L.W. Seeb. 2014. Signals of heterogeneous selection at an MHC locus in geographically proximate ecotypes of sockeye salmon. Coastwide Salmon Genetics Meeting. Larson, W.A., J.E. Seeb, and L.W. Seeb. 2013. Using RAD sequencing to improve GSI of Chinook salmon from western Alaska. Annual meeting of the Western Division of the American Fisheries Society. Larson, W.A., C.E. Pascal, J.E. Seeb, and L.W. Seeb. 2012. Resolving population structure and improving management of western Alaskan Chinook salmon using next-generation sequencing. 1st Pan-Pacific Advanced Studies Institute. Larson, W.A. 2012. Obtaining accurate allele frequencies from RAD data. 1st Pan-Pacific Advanced Studies Institute. Larson, W. A., F. M. Utter, K. W. Myers, W. D. Templin, J. E. Seeb, and L. W. Seeb. 2011. Natal origins, distributions and migratory patterns of Chinook salmon in the Bering Sea and North Pacific Ocean. Annual meeting of the Alaska Chapter of the American Fisheries Society.

Teaching Topics in Conservation Genetics. Location: University of Wisconsin-Stevens Point, code: NRES775, taught: spring 2018, 1 credits, graduate level, 5 students. R Programming for Biologists. Location: University of Wisconsin-Stevens Point, code: NRES605, taught spring 2017 and offered spring 2019, 3 credits, graduate level, ~15 students.

Student and post-doctoral training Post-doctoral fellows

W.A Larson 6

Peter Euclide, University of Wisconsin-Stevens Point, 2018-present. Amanda Ackiss, University of Wisconsin-Stevens Point, 2018-present.

Graduate students (committee chair) Becky Gehri University of Wisconsin-Stevens Point, MS, 2018-present. Thesis title: Genetic assessment of Boardman River fish populations prior to dam removal Danielle Blumstein, University of Wisconsin-Stevens Point, MS, 2017-present. Thesis title: Development of a genetic linkage map for cisco (Coregonus artedi) to facilitate integrated studies of adaptive diversity Matthew Bootsma, University of Wisconsin-Stevens Point, MS, 2017-present. Thesis title: Developing a high-throughput SNP panel to facilitate genetic stock identification of walleye (Sander Vitreus) in Wisconsin and Minnesota Jenna Ruzich, University of Wisconsin-Stevens Point, MS, 2016-2018. Thesis title: Genetic assessment of seven fish species above and below the Prairie du Sac Dam on the Wisconsin River.

Graduate students (committee member) Bradley Erdman, University of Maine, PhD, 2016-present. Jamie Dobosenski, University of Minnesota-Duluth, MS, 2016-2018. Eric Wegleitner, University of Wisconsin-Stevens Point, MS, 2016-2018. Jason Gostiaux, University of Wisconsin-Stevens Point, MS, 2016-2018. Michael Vaske, University of Wisconsin-Stevens Point, MS, 2015-2017.

Undergraduate students (directed research) Ben Schleppenbach, University of Wisconsin-Stevens Point, 2016-present. Tommy Hill, University of Wisconsin-Stevens Point, 2017-present. Bradley Erdman, University of Wisconsin-Stevens Point, 2016. Robert Hall, University of Washington, 2011.

University service Member of University Wisconsin-Stevens Point Personnel Development Committee, 2018-present Co-organizer, Great Lakes Fish and Wildlife Spring Seminar Series, 2018

Other professional activities Journal referee: Global Change Biology, Proceedings of the Royal Society B, Molecular Ecology, Molecular Ecology Resources, Evolutionary Applications, Canadian Journal of Fisheries and Aquatic Sciences, ICES Journal of Marine Science, Transactions of the American Fisheries Society, North American Journal of Fisheries Management, PLoS One, Reviews in Fish Biology and Fisheries, Journal of Fish Biology, Ecology and Evolution, Journal of Heredity, Heredity, Journal of Great Lakes Research, Aquatic Conservation: Marine and Freshwater Ecosystems, Management of Biological Invasions, Hydrobiologia, Biological Invasions, Managing Centrarchid Fisheries (book chapter, book published by the American Fisheries Society), and Ecology and Management of Lake Charr (book chapter, book to be published by Springer). Competitive grant referee: Great Lakes Fishery Commission Fishery Research Program, Delta

W.A Larson 7

Water Quality and Ecosystem Restoration Grant Program, Genome Quebec, Mitacs Accelerate Research Program, the Centres de Ressources Biologiques pour les Animaux Domestiques, the Legislative-Citizen Commission on Minnesota Resources, and the Natural Sciences and Engineering Research Council of Canada. External reviewer for the Recovery Plan for the Northern Population Segment of the copperbelly watersnake (Nerodia erythrogaster neglecta) prepared by the U.S. Fish and Wildlife Service. Symposium co-organizer, “Application of genomic tools to inform management of the Great Lakes” to be held at the 2019 International Association for Great Lakes Research Annual Meeting in Brockport, NY Member, Great Lakes Fishery Commission FishPass Science Advisory Team Co-webmaster, Genetics Section of the American Fisheries Society website (2014-2018) Judge: Phelps Award for Best Genetics Paper Published in an AFS Journal (2017, 2018); AFS Early Career Professional Travel Award (2017, 2018) Secretary Treasurer, AFS Genetics Section (2018-2020)

Memberships American Fisheries Society, member since July 2011. Member of the Genetics Section (2011- present), Washington-British Columbia Chapter (2011-2015), Alaska Chapter (2011-2015), Western Division (2011-2015), Student Subsection (2011-2015), North Central Division (2016-present), and Wisconsin Chapter (2016-present) International Association for Great Lakes Research, member since 2017. American Institute of Fishery Research Biologists, member since 2016. American Genetic Association, member since 2015.

W.A Larson 8