BISWADIP DAS, Ph.D. JADAVPUR UNIVERSITY, DEPARTMENT OF LIFE SCIENCE AND BIOTECHNOLOGY, 188 RAJA S.C. MULLICK ROAD, KOLKATA - 700 032, INDIA TEL: 919748908607, FAX: 91-33-2414-6414, E-MAIL:[email protected]

OBJECTIVE To teach Molecular Biology and Genetics at Postgraduate level and conduct research on Molecular Biology and genetics with special reference to quality control and surveillance of eukaryotic gene expression and mRNA processing and degradation using genetics, molecular biology, genomics and system biology platform and implications of these processes in different genetic diseases in human.

EDUCATION • Doctor of Philosophy (Ph.D.) in Biophysics, Molecular Biology and genetics, 1996, University of Calcutta, India. • Master of Science (M. Sc.) in Biophysics, Molecular Biology and Genetics, 1989, University of Calcutta, India. • Bachelor of Science (B. Sc.) in Zoology, Chemistry and Physiology Minor, 1987, University of Calcutta, India

AWARDS, HONORS AND ACCOMPLISHMENTS

• Named INSA Award for Young Scientist awarded by Indian National Science Academy, Government of India in 1997. • Awarded “Young Scientist Research Grant” from Indian National Science Academy entitled “Pathogenecity in Candida albicans” in 1997. • Ranked within the top 10 Candidates (out of approximately 10,000 candidates) in a National Eligibility Test as conducted by Council of Scientific and Industrial Research, Government of India in 1990. • Ranked 2nd in MS in the University Of Calcutta, India in 1989. • Ranked 1st in BS and awarded Jubilee Merit Fellowship by University of Calcutta, India in 1987. • Trained 9 Ph.D., and 5 technicians.

TEACHING EXPERIENCE

• Teaching courses entitled “Cell Biology”, “Recombinant DNA Technology” and “Molecular Genetics, Genomics and Proteomics”, to the postgraduate students under Masters Program in Life Sciences and Biotechnology, Jadavpur University and “Molecular Biology” to the postgraduate students under Masters Program in Biophysics and Molecular Biology, University of Calcutta.

• Teaching courses entitled “Advanced Molecular Biology”, “Recombinant DNA Technology and Genomics” and “Microbial Genetics and Genomics” to the postgraduate students under Masters Program of West Bengal State University.

• Teaching undergraduate course entitled “Gene Expression in Salivary Glands” to the pre-dental undergraduate students under the “Summer Learning Program” of University of Florida.

• Participated in Career Internship Program of Pittsford Central School, Pittsford, NY and served as External Advisor.

PUBLICATIONS

1. Das, B. and Sherman, F. Interrelationship between different nuclear mRNA decay pathways (In preparation).

2. Das, S., Das, B., Gao, Q., and Sherman, F. Cbc2p, Upf3p and eIF4G are components of DRN (degradation of mRNA in the nucleus) pathway in S. cerevisiae (In Preparation).

3. Das, B., Cash, M.N., Eliot, R. and Culp, D.J. sld Mutation: Genetic Mapping and Functional Assessment. (In Preparation).

4. Das, B., Cash, M.N., Hand, A.R., Shivazad, A., Grieshaber, S.S., Robinson, B., and Culp, D.J. (2010) Tissue Distribution of Murine Muc19/Smgc gene products. J. Histochem. Cytochem, 58, 141-156

5. Das, B., Cash, M. N., Hand, A. R., Shivazad, A., and Culp, D. J. (2009) Expression of Muc19/Smgc Gene Products During Murine Sublingual Gland Development: Cytodifferentiation and Maturation of Salivary Mucous Cells J. Histochem. Cytochem.,57,383-396.

6. Das, B., Das, S., and Sherman, F. (2006) Mutant lys2 mRNAs retained and degraded in the nucleus of Saccharomyces cerevisiae Proc. Natl. Acad. Sci USA,103, 10871-10876.

*7. Kuai, L., Das, B. and Sherman, F. (2005) A nuclear degradation pathway controls the abundance of normal mRNAs in Saccharomyces cerevisiae Proc. Natl. Acad. Sci USA, 102, 13962-13967. * This is an equal contribution paper.

8. Gao, Q., Das, B., Sherman, F. and Maquat, L.E. (2005) Cap-binding protein 1-mediated and eukaryotic translation initiation factor 4E-mediated pioneer rounds of translation in yeast. Proc. Natl. Acad. Sci. USA, 102, 4258-4263.

9. Wang, Y-K., Das, B, Huber, D. H., Sherman, F. and Ruschenko, E. P. (2004) Role of the 14-3-3 protein in carbon metabolism of the pathogenic yeast Candida albicans. Yeast, 21, 685-702.

10. Das, B., Butler, J.S. and Sherman, F. (2003) Degradation of normal mRNA in the nucleus of yeast. Mol. Cell Biol, 23, 5502-5515

11. Das, B., Guo, Z., Russo, P., Chartrand, P., and Sherman, F (2000). The Role of Nuclear Cap Binding Protein Cbc1p of Yeast in mRNA Termination and Degradation. Mol. Cell Biol., 20,. 2827-2838.

12. Polevoda, B., Martzen, M., Das, B., Phizicky, E.M., and Sherman, F. (2000) Cytochrome c methyl- transferase, Ctm1p of yeast. J. Biol. Chem., 275, 20508-20513

13. Pearce, D.A., Carr, C. J., Das, B. and Sherman, F. (1999). Phenotypic reversal of btn1 defects in yeast by chloroquine : A yeast model for batten disease. Proc. Natl. Acad. Sci USA , 96, 11341-11345

14. Chakrabarti, A., Bera, A.K., Das, B., Chattopadhyay, C., Sarkar, D. and DasGupta, C. (1999) Curr. Sci., 76, 1235-1238

15. Pearce, D.A., Ferea, T., Nosel, S. A., Das, B. and Sherman, F (1999) Action of BTN1, the yeast orthologue of the gene mutated in Batten disease. Nature Genet., 22, 55-58.

16. Chattopadhyay, S., Das, B., and DasGupta, C. (1996) Reactivation of denatured proteins by 23S ribosomal RNA: Role of domain V. Proc. Natl. Acad. Sci. USA, 93, 8284-82871.

17. Das, B., Chattopadhyay, S., Bera, A.K. and DasGupta, C. (1996). In vitro protein folding by ribosomes from Escherichia coli, wheat germ and rat liver: The role of 50S subunit and its 23S rRNA. Eur. J. Biochem., 235,613-62 1

18. Bera A. K., Das, B., Chattopadhyay, S. and DasGupta, C. (1994). Refolding of denatured restriction endonucleases with ribosomal preparations from Methanosarcina barkeri. Biochem. Mol. Biol. International.,32, 315-323

19. Chattopadhyay, S., Das, B., Bera, A. K. and DasGupta, C. (1994) Refolding of denatured lactate dehydrogenase by E. coli ribosome. Biochem. J. (London), 300, 717-721

20. Bera, A. K., Das, B., Chattopadhyay, S. and DasGupta, C. (1994) Protein folding by ribosome and its RNA. Curr. Sci., 66, 230-232.

21. Das, B. and DasGupta, C.(1992) Reconstitution of denatured alkaline phosphatase with E. coli ribosome. Ind. J. Biochem. Biophys., 29, 512-515.

22. Das, B., Chattopadhyay, S. and DasGupta, C. (1992) Reactivation of denatured fungal glucose 6-phosphate dehydrogenase and E. coli alkaline phosphatase with E. coli ribosome. Biochem. Biophys. Res. Commun., 183, 774-780.

23 Maity, S., Das, B. and Mandal, N.C. (1991) Isolation and preliminary characterization of mutants of Escherichia coli resistant to lethal action of bacteriophage lambda P gene. Virology, 182, 351-352.

PROFESSIONAL EXPERIENCE

● Served as Chairperson of Research Committee (Biotechnology) of the Jadavpur University.

 Served as Chairperson of Postgraduate Board of Studies (Biotechnology) of the Jadavpur University.

 Served as Chairperson of Undergraduate Board of Studies (Microbiology) of the West Bengal State University.

● Served as Chairperson of Postgraduate Board of Studies (Microbiology) of the West Bengal State University.

● Served as Chairperson of Ph.D. Committee (Microbiology) of the West Bengal State University.

● Served as Member-Secretary of Biosafety Committee of West Bengal State University.

● Served as Member of the Inspection team of West Bengal State University to oversee the Infrastructure of various undergraduate colleges offering B. Sc. And M.Sc. in Microbiology.

● Served as Expert of selection committees conducting interviews for selection of lecturers in Microbiology in undergraduate levels.

TECHNICAL EXPERIENCE

● Genomics (Whole Genome Expression Profiling using Oligonucleotide Microarray Analysis, in silico analysis of genome and proteome using different Bioinformatic tools).

● Proteomics (Analysis of Multiprotein Complexes using Tandem Affinity Purification (TAP), Protein-protein interaction by co-immunoprecipitation (Co-IP) and Yeast Two hybrid system,

● Molecular Biology and Genetics (Core and State-of the art and high throughput techniques of Molecular Biology e.g. complex gene expression analysis Real Time quantitative RT-PCR, Chromatin Immunoprecipitation – ChIP, Northern analysis, Western and RPA analysis and Biochemical techniques of RNA, all kinds of in vitro, random and targeted mutagenesis, Gene disruption and knock-out techniques in bacteria, yeast and mouse).

● Cell Biology (Animal cell and tissue culture, Fluorescence In Situ Hybridization of RNA, Single Species mRNA localization by GFP Imaging)

● Protein Chemistry (Expression and Purification of Proteins, Folding of Proteins by different spectroscopic methods).

● Classical and Molecular Genetics of mouse, yeast and E. coli and bacteriophage lambda.

● Biochemical Techniques of Ribosome and Ribosomal RNA

ACADEMIC POSITIONS HELD

JADAVPUR UNIVERSITY, DEPARTMENT OF LIFE SCIENCE AND BIOTECHNOLOGY, KOLKATA, INDIA Reader in Life Science and Biotechnology, 07/2010-Till Date: Serving as Reader in Life Science and Biotechnology. Primary duties involve Teaching and Research Program in Masters and Doctoral Level.

WEST BENGAL STATE UNIVERSITY, DEPARTMENT OF MICROBIOLOGY, KOLKATA, Reader in Microbiology, 04/2009-07/2010: Served as Reader and Head of the Department of Microbiology besides Teaching and Research Program in Masters and Doctoral Level. In addition, took major administrative roles in developing the department as well as managing various academic activities such as conducting the honours and general examinations of the university at the undergraduate level. Holding various portfolios in the key administrative and academic committees as mentioned above.

UNIVERSITY OF FLORIDA, DEPARTMENT OF ORAL BIOLOGY, COLLEGE OF DENTISTRY, GAINESVILLE, FL, USA. Research Assistant Professor, 05/2006 – 04/2009: Involved in the investigation of regulation of expression of mucin gene, splicing and degradation of mucin mRNA in salivary glands in mouse and human using Real Time PCR, genomics, proteomics, molecular biology, genetics and other techniques.

UNIVERSITY OF ROCHESTER, DEPARTMENT OF BIOPHYSICS AND BIOCHEMISTRY, SCHOOL OF MEDICINE AND DENTISTRY, ROCHESTER, NY, USA. Research Assistant Professor, 02/2002 –04/2006. Postdoctoral Research Associate, 12/1997 – 01/2002: Involved in the investigation of DRN, a novel mRNA degradation pathway in nucleus and its molecular characterization in yeast using cell biological, biochemical and molecular genetic tools and techniques.

JAWAHARLAL NEHRU UNIVERSITY, CENTRE FOR PLANT MOLECULAR BIOLOGY, SCHOOL OF LIFE SCIENCES, NEW DELHI, INDIA. Research Associate, 6/1996 - 11/1997. Senior Research Fellow: 1/1996 - 5/1996: Dealt with identification of genes and virulent factors playing a role in the morphogenesis of a human pathogenic fungus Candida albicans.

UNIVERSITY OF CALCUTTA, DEPARTMENT OF BIOPHYSICS, MOLECULAR BIOLOGY AND GENETICS, CALCUTTA, INDIA. Senior Research Fellow: 9/1992 - 8/1995. Junior Research Fellow: 8/1990 - 8/1992: Involved in the studies dealing with the functional analysis of ribosomes and 23S ribosomal RNA which led to the discovery of the protein folding activity of these biomolecules.

BOSE INSTITUTE, DEPARTMENT OF BIOCHEMISTRY, CALCUTTA, INDIA. Research Trainee : 1/1990 - 4/1990: Completed a project as part of the curriculum of M. Sc. course involving the isolation of a mutant strain of Escherichia coli resistant to the lethal host killing property of bacteriophage lambda.

CONFERENCE ATTENDED

7th RNA Group Meeting at IISc,Bangalore, India (30/03/12-31/03/12): Presented an invited oration Entitled "Killing messengers before they were born: A paradigm for cell survival and gene regulation."

6th International Meeting on Yeast Biology at IIT, Mumbai, India (10/12/11-15/12/11): Presented an invited oration Entitled "Killing messengers before they were born: A paradigm for cell survival and gene regulation."

80th Annual General Body Meeting of the Society for Biological Chemists, at Lucknow, India (12/11/11-15/12/11): Presented a poster Entitled "Killing messengers before they were born"

FASEB Experimental Biology Meeting at Washington, D.C. (04/28/07-05/02/07) presented a poster entitled “Salivary mucous gland gene expression: Identifying the gene harboring the sld mutation”.

Northeast Regional Yeast Meeting, University of Rochester (10/24/04) presented a talk entitled “The nuclear degradation pathway (DRN) controls the abundance of normal yeast mRNAs”

Northeast Regional Yeast Meeting, McGill University, Montreal, Quebec, Canada (07/27/03- 07/28/03) presented a talk entitled “Degradation of normal mRNA in the nucleus

Eukaryotic mRNA processing meeting at Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (08/22/01-08/26/01) presented a talk entitled “A Novel Nuclear mRNA Decay Pathway of Yeast”.

Northeast Regional Yeast Meeting, University of Toronto, Toronto, Ontario, Canada (07/13/01-07/14/01) presented a poster entitled “A Novel Nuclear mRNA Decay System of Yeast”.

Annual Buffalo RNA Graduate Group Spring Forum, State University of New York, Buffalo, NY (4/3/01) presented a talk entitled “A Novel Nuclear mRNA Decay System of Yeast”.

Northeast Regional Yeast Meeting, State University of New York, Syracuse, NY (6/9/00): Presented a talk entitled "Nuclear Cap Binding Protein, Cbc1p is involved in mRNA degradation in the nucleus of Yeast Saccharomyces cerevisiae". mRNA Decay Meeting, Jackson Hole, WY,USA (10/06/99 - 10/10/99): Presented a talk entitled the "The Role of Nuclear cap Binding Protein, Cbc1p of Yeast in mRNA Termination and Degradation".

Northwest Regional Yeast Meeting, State University of New York, Buffalo, NY (11/7/99): Presented a talk entitled " The role of Nuclear Cap Binding Protein, Cbc1p in mRNA degradation in Yeast Saccharomyces cerevisiae".

Sixteenth International Congress organized by IUBMB, New Delhi, India (09/18/94- 09/22/94): Presented Poster Entitled "Protein Folding Activities of Ribosomes".

Diamond Jubilee and Annual General Body Meeting of the Society For Biological Chemists, India (12/27/91-12/30/91): Presented an Invited Oration Entitled "Refolding of denatured lactate dehydrogenase by E. coli ribosome" REFERENCES

Prof. Chanchal K. DasGupta Department of Biophysics, Molecular Biology and Genetics University College of Science University of Calcutta 92, A.P.C. Rd., Kolkata, 700 071, India Tel : 91-33-2359-1705 E. Mail: [email protected]

Prof Fred Sherman. Department of Biochemistry and Biophysics University of Rochester School of Medicine and Dentistry 601 Elmwood Avenue, Box 712,Rochester, NY 14642, USA Tel: 585-275-6647, FAX: 585-275-6007 E. mail: [email protected]

Prof. J. Scott Butler Department of Microbiology and Immunology University of Rochester School of Medicine and Dentistry 601 Elmwood Avenue, Box 672, Rochester, NY 14642, USA Tel: 585-275-7921, FAX: 585-472-9575 E. mail: [email protected]

Prof. Lynne E. Maquat Department of Biochemistry and Biophysics University of Rochester School of Medicine and Dentistry 601 Elmwood Avenue, Box 612, Rochester, NY 14642 Tel: 585-273-5640, FAX: 585-271-2683 E. mail: [email protected]

Prof. David J. Culp Department of Oral Biology College of Dentistry University of Florida 1600 SW Archer Road, P.O. Box 100424 Gainesville, FL 32610 Tel: 352-846-0800, FAX: 585-271-2683 E.mail: [email protected].