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Research Article Isolation and Identification of Endophytic Fungi from Actinidia Macrosperma and Investigation of Their Bioactiv
Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine Volume 2012, Article ID 382742, 8 pages doi:10.1155/2012/382742 Research Article Isolation and Identification of Endophytic Fungi from Actinidia macrosperma and Investigation of Their Bioactivities Yin Lu,1 Chuan Chen,2 Hong Chen,1 Jianfen Zhang,1 and Weiqin Chen1 1 College of Biological and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, China 2 Hangzhou Botanical Garden, Hangzhou 310013, China Correspondence should be addressed to Yin Lu, [email protected] Received 12 May 2011; Revised 28 August 2011; Accepted 11 September 2011 Academic Editor: Andreas Sandner-Kiesling Copyright © 2012 Yin Lu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Endophytic fungi from the Chinese medicinal plant Actinidia macrosperma were isolated and identified for the first time. This was the first study to evaluate their cytotoxic and antitumour activities against brine shrimp and five types of tumour cells, respectively. In total, 17 fungal isolates were obtained. Five different taxa were represented by 11 isolates, and six isolates were grouped into the species of Ascomycete Incertae sedis with limited morphological and molecular data. Cytotoxic activity has been found in most isolates except AM05, AM06, and AM10. The isolates AM07 (4.86 µg/mL), AM11 (7.71 µg/mL), and AM17 (14.88 µg/mL) exhibited significant toxicity against brine shrimp. The results of the MTT assay to assess antitumour activity revealed that 82.4% of isolate fermentation broths displayed growth inhibition (50% inhibitory concentration IC50 < 100 µg/mL). -
The Evolution of Secondary Metabolism Regulation and Pathways in the Aspergillus Genus
THE EVOLUTION OF SECONDARY METABOLISM REGULATION AND PATHWAYS IN THE ASPERGILLUS GENUS By Abigail Lind Dissertation Submitted to the Faculty of the Graduate School of Vanderbilt University in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY in Biomedical Informatics August 11, 2017 Nashville, Tennessee Approved: Antonis Rokas, Ph.D. Tony Capra, Ph.D. Patrick Abbot, Ph.D. Louise Rollins-Smith, Ph.D. Qi Liu, Ph.D. ACKNOWLEDGEMENTS Many people helped and encouraged me during my years working towards this dissertation. First, I want to thank my advisor, Antonis Rokas, for his support for the past five years. His consistent optimism encouraged me to overcome obstacles, and his scientific insight helped me place my work in a broader scientific context. My committee members, Patrick Abbot, Tony Capra, Louise Rollins-Smith, and Qi Liu have also provided support and encouragement. I have been lucky to work with great people in the Rokas lab who helped me develop ideas, suggested new approaches to problems, and provided constant support. In particular, I want to thank Jen Wisecaver for her mentorship, brilliant suggestions on how to visualize and present my work, and for always being available to talk about science. I also want to thank Xiaofan Zhou for always providing a new perspective on solving a problem. Much of my research at Vanderbilt was only possible with the help of great collaborators. I have had the privilege of working with many great labs, and I want to thank Ana Calvo, Nancy Keller, Gustavo Goldman, Fernando Rodrigues, and members of all of their labs for making the research in my dissertation possible. -
Fungal Diversity Driven by Bark Features Affects Phorophyte
www.nature.com/scientificreports OPEN Fungal diversity driven by bark features afects phorophyte preference in epiphytic orchids from southern China Lorenzo Pecoraro1*, Hanne N. Rasmussen2, Sofa I. F. Gomes3, Xiao Wang1, Vincent S. F. T. Merckx3, Lei Cai4 & Finn N. Rasmussen5 Epiphytic orchids exhibit varying degrees of phorophyte tree specifcity. We performed a pilot study to investigate why epiphytic orchids prefer or avoid certain trees. We selected two orchid species, Panisea unifora and Bulbophyllum odoratissimum co-occurring in a forest habitat in southern China, where they showed a specifc association with Quercus yiwuensis and Pistacia weinmannifolia trees, respectively. We analysed a number of environmental factors potentially infuencing the relationship between orchids and trees. Diference in bark features, such as water holding capacity and pH were recorded between Q. yiwuensis and P. weinmannifolia, which could infuence both orchid seed germination and fungal diversity on the two phorophytes. Morphological and molecular culture-based methods, combined with metabarcoding analyses, were used to assess fungal communities associated with studied orchids and trees. A total of 162 fungal species in 74 genera were isolated from bark samples. Only two genera, Acremonium and Verticillium, were shared by the two phorophyte species. Metabarcoding analysis confrmed the presence of signifcantly diferent fungal communities on the investigated tree and orchid species, with considerable similarity between each orchid species and its host tree, suggesting that the orchid-host tree association is infuenced by the fungal communities of the host tree bark. Epiphytism is one of the most common examples of commensalism occurring in terrestrial environments, which provides advantages, such as less competition and increased access to light, protection from terrestrial herbivores, and better fower exposure to pollinators and seed dispersal 1,2. -
Purpureocillium Lilacinum and Metarhizium Marquandii As Plant Growth-Promoting Fungi
Purpureocillium lilacinum and Metarhizium marquandii as plant growth-promoting fungi Noemi Carla Baron1, Andressa de Souza Pollo2 and Everlon Cid Rigobelo1 1 Agricultural and Livestock Microbiology Graduation Program, São Paulo State University (UNESP), School of Agricultural and Veterinarian Sciences, Jaboticabal, São Paulo, Brazil 2 Department of Preventive Veterinary Medicine and Animal Reproduction, São Paulo State University (UNESP), School of Agricultural and Veterinarian Sciences, Jaboticabal, São Paulo, Brazil ABSTRACT Background: Especially on commodities crops like soybean, maize, cotton, coffee and others, high yields are reached mainly by the intensive use of pesticides and fertilizers. The biological management of crops is a relatively recent concept, and its application has increased expectations about a more sustainable agriculture. The use of fungi as plant bioinoculants has proven to be a useful alternative in this process, and research is deepening on genera and species with some already known potential. In this context, the present study focused on the analysis of the plant growth promotion potential of Purpureocillium lilacinum, Purpureocillium lavendulum and Metarhizium marquandii aiming its use as bioinoculants in maize, bean and soybean. Methods: Purpureocillium spp. and M. marquandii strains were isolated from soil samples. They were screened for their ability to solubilize phosphorus (P) and produce indoleacetic acid (IAA) and the most promising strains were tested at greenhouse in maize, bean and soybean plants. Growth promotion parameters including plant height, dry mass and contents of P and nitrogen (N) in the plants and Submitted 18 December 2019 in the rhizospheric soil were assessed. Accepted 27 March 2020 Results: Thirty strains were recovered and characterized as Purpureocillium Published 27 May 2020 lilacinum (25), Purpureocillium lavendulum (4) and Metarhizium marquandii Corresponding author (1). -
Structural Diversity in Echinocandin Biosynthesis: the Impact of Oxidation Steps and Approaches Toward an Evolutionary Explanation
Z. Naturforsch. 2017; 72(1-2)c: 1–20 Wolfgang Hüttel* Structural diversity in echinocandin biosynthesis: the impact of oxidation steps and approaches toward an evolutionary explanation DOI 10.1515/znc-2016-0156 can be well applied to parts of the pathway; however, thus Received July 29, 2016; revised July 29, 2016; accepted August 28, far, there is no comprehensive theory that could explain 2016 the entire biosynthesis. Abstract: Echinocandins are an important group of cyclic Keywords: antifungals; gene clusters; metabolic diversity; non-ribosomal peptides with strong antifungal activ- non-ribosomal peptide biosynthesis; secondary meta- ity produced by filamentous fungi from Aspergillaceae bolism; filamentous fungi. and Leotiomycetes. Their structure is characterized by numerous hydroxylated non-proteinogenic amino acids. Biosynthetic clusters discovered in the last years contain up to six oxygenases, all of which are involved in amino 1 Introduction acid modifications. Especially, variations in the oxidation Echinocandins are fungal non-ribosomal cyclic hexapep- pattern induced by these enzymes account for a remark- tides with a fatty acid side chain attached to a dihydroxy- able structural diversity among the echinocandins. This ornithine residue. As they are specific noncompetitive review provides an overview of the current knowledge of inhibitors of the β-1,3-glucan synthase involved in fungal echinocandin biosynthesis with a special focus on diver- cell wall biosynthesis, they have a pronounced antifungal sity-inducing oxidation steps. The emergence of metabolic bioactivity. Although natural echinocandins are not of diversity is further discussed on the basis of a comprehen- clinical use due to their toxicity and low solubility, chemi- sive overview of the structurally characterized echinocan- cal derivatives such as caspofungin, anidulafungin, and dins, their producer strains and biosynthetic clusters. -
Taxonomy, Chemodiversity, and Chemoconsistency of Aspergillus, Penicillium, and Talaromyces Species
View metadata,Downloaded citation and from similar orbit.dtu.dk papers on:at core.ac.uk Dec 20, 2017 brought to you by CORE provided by Online Research Database In Technology Taxonomy, chemodiversity, and chemoconsistency of Aspergillus, Penicillium, and Talaromyces species Frisvad, Jens Christian Published in: Frontiers in Microbiology Link to article, DOI: 10.3389/fmicb.2014.00773 Publication date: 2015 Document Version Publisher's PDF, also known as Version of record Link back to DTU Orbit Citation (APA): Frisvad, J. C. (2015). Taxonomy, chemodiversity, and chemoconsistency of Aspergillus, Penicillium, and Talaromyces species. Frontiers in Microbiology, 5, [773]. DOI: 10.3389/fmicb.2014.00773 General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights. • Users may download and print one copy of any publication from the public portal for the purpose of private study or research. • You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. MINI REVIEW ARTICLE published: 12 January 2015 doi: 10.3389/fmicb.2014.00773 Taxonomy, chemodiversity, and chemoconsistency of Aspergillus, Penicillium, and Talaromyces species Jens C. Frisvad* Section of Eukaryotic Biotechnology, Department of Systems Biology, Technical University of Denmark, Kongens Lyngby, Denmark Edited by: Aspergillus, Penicillium, and Talaromyces are among the most chemically inventive of Jonathan Palmer, United States all fungi, producing a wide array of secondary metabolites (exometabolites). -
Diversity and Saline Resistance of Endophytic Fungi Associated with Pinus Thunbergii in Coastal Shelterbelts of Korea Young Ju Min1, Myung Soo Park1, Jonathan J
J. Microbiol. Biotechnol. (2014), 24(3), 324–333 http://dx.doi.org/10.4014/jmb.1310.10041 Research Article jmb Diversity and Saline Resistance of Endophytic Fungi Associated with Pinus thunbergii in Coastal Shelterbelts of Korea Young Ju Min1, Myung Soo Park1, Jonathan J. Fong1, Ying Quan1, Sungcheol Jung2, and Young Woon Lim1* 1School of Biological Sciences, Seoul National University, Seoul 151-747, Republic of Korea 2Warm-Temperate and Subtropical Forest Research Center, KFRI, Seogwipo 697-050, Republic of Korea Received: October 14, 2013 Revised: November 29, 2013 The Black Pine, Pinus thunbergii, is widely distributed along the eastern coast of Korea and its Accepted: December 4, 2013 importance as a shelterbelt was highlighted after tsunamis in Indonesia and Japan. The root endophytic diversity of P. thunbergii was investigated in three coastal regions; Goseong, Uljin, and Busan. Fungi were isolated from the root tips, and growth rates of pure cultures were First published online measured and compared between PDA with and without 3% NaCl to determine their saline December 9, 2013 resistance. A total of 259 isolates were divided into 136 morphotypes, of which internal *Corresponding author transcribed spacer region sequences identified 58 species. Representatives of each major fungi Phone: +82-2-880-6708; phylum were present: 44 Ascomycota, 8 Zygomycota, and 6 Basidiomycota. Eighteen species Fax: +82-2-871-5191; exhibited saline resistance, many of which were Penicillium and Trichoderma species. Shoreline E-mail: [email protected] habitats harbored higher saline-tolerant endophytic diversity compared with inland sites. This investigation indicates that endophytes of P. thunbergii living closer to the coast may have pISSN 1017-7825, eISSN 1738-8872 higher resistance to salinity and potentially have specific relationships with P. -
Aspects of the Biology of Entomogenous Fungi And
ASPECTS OF THE BIOLOGY OF ENTOMOGENOUS FUNGI AND THEIR ASSOCIATIONS WITH ARTHROPODS By EDUARDO MELITON JOVEL AYALA B.Sc. Agronomy, National School of Agriculture, El Salvador 1980 B.Sc. Botany, California State Polytechnic University, Pomona, California, USA 1993 M.Sc. Ethnobotany, University of British Columbia, Vancouver, Canada 1996 A THESIS SUBMITTED IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR IN PHILOSOPHY In THE FACULTY OF GRADUATE STUDIES Department of Botany We accept this thesis as conforming to the required standards THE UNIVERSITY OF BRITISH COLUMBIA August 2002 © Eduardo M. Jovel Ayala, 2002 In presenting this thesis in partial fulfilment of the requirements for an advanced degree at the University of British Columbia, I agree that the Library shall make it freely available for reference and study. I further agree that permission for extensive copying of this thesis for scholarly purposes may be granted by the head of my department or by his or her representatives. It is understood that copying or publication of this thesis for financial gain shall not be allowed without my written permission. Department of The University of British Columbia Vancouver, Canada II ABSTRACT I investigated several aspects of the biology of entomogenous fungi (mostly Clavicipitaceae with few species of Hypocreaceae). My primary motive in this research was to gain an understanding of the interactions between entomogenous fungi and arthropods. My study included field collections and identification of entomogenous fungi from BC and a few collections from Peru and Idaho. I addressed some aspects of the interactions among arthropods and fungi, life histories of fungi under laboratory conditions, and observations of chemical changes of fungi growing in the presence of heavy metals. -
Identification and Nomenclature of the Genus Penicillium
Downloaded from orbit.dtu.dk on: Dec 20, 2017 Identification and nomenclature of the genus Penicillium Visagie, C.M.; Houbraken, J.; Frisvad, Jens Christian; Hong, S. B.; Klaassen, C.H.W.; Perrone, G.; Seifert, K.A.; Varga, J.; Yaguchi, T.; Samson, R.A. Published in: Studies in Mycology Link to article, DOI: 10.1016/j.simyco.2014.09.001 Publication date: 2014 Document Version Publisher's PDF, also known as Version of record Link back to DTU Orbit Citation (APA): Visagie, C. M., Houbraken, J., Frisvad, J. C., Hong, S. B., Klaassen, C. H. W., Perrone, G., ... Samson, R. A. (2014). Identification and nomenclature of the genus Penicillium. Studies in Mycology, 78, 343-371. DOI: 10.1016/j.simyco.2014.09.001 General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights. • Users may download and print one copy of any publication from the public portal for the purpose of private study or research. • You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. available online at www.studiesinmycology.org STUDIES IN MYCOLOGY 78: 343–371. Identification and nomenclature of the genus Penicillium C.M. -
Characterization of Terrelysin, a Potential Biomarker for Aspergillus Terreus
Graduate Theses, Dissertations, and Problem Reports 2012 Characterization of terrelysin, a potential biomarker for Aspergillus terreus Ajay Padmaj Nayak West Virginia University Follow this and additional works at: https://researchrepository.wvu.edu/etd Recommended Citation Nayak, Ajay Padmaj, "Characterization of terrelysin, a potential biomarker for Aspergillus terreus" (2012). Graduate Theses, Dissertations, and Problem Reports. 3598. https://researchrepository.wvu.edu/etd/3598 This Dissertation is protected by copyright and/or related rights. It has been brought to you by the The Research Repository @ WVU with permission from the rights-holder(s). You are free to use this Dissertation in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you must obtain permission from the rights-holder(s) directly, unless additional rights are indicated by a Creative Commons license in the record and/ or on the work itself. This Dissertation has been accepted for inclusion in WVU Graduate Theses, Dissertations, and Problem Reports collection by an authorized administrator of The Research Repository @ WVU. For more information, please contact [email protected]. Characterization of terrelysin, a potential biomarker for Aspergillus terreus Ajay Padmaj Nayak Dissertation submitted to the School of Medicine at West Virginia University in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Immunology and Microbial Pathogenesis Donald H. Beezhold, -
Identification and Nomenclature of the Genus Penicillium
available online at www.studiesinmycology.org STUDIES IN MYCOLOGY 78: 343–371. Identification and nomenclature of the genus Penicillium C.M. Visagie1, J. Houbraken1*, J.C. Frisvad2*, S.-B. Hong3, C.H.W. Klaassen4, G. Perrone5, K.A. Seifert6, J. Varga7, T. Yaguchi8, and R.A. Samson1 1CBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, NL-3584 CT Utrecht, The Netherlands; 2Department of Systems Biology, Building 221, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark; 3Korean Agricultural Culture Collection, National Academy of Agricultural Science, RDA, Suwon, Korea; 4Medical Microbiology & Infectious Diseases, C70 Canisius Wilhelmina Hospital, 532 SZ Nijmegen, The Netherlands; 5Institute of Sciences of Food Production, National Research Council, Via Amendola 122/O, 70126 Bari, Italy; 6Biodiversity (Mycology), Agriculture and Agri-Food Canada, Ottawa, ON K1A0C6, Canada; 7Department of Microbiology, Faculty of Science and Informatics, University of Szeged, H-6726 Szeged, Közep fasor 52, Hungary; 8Medical Mycology Research Center, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8673, Japan *Correspondence: J. Houbraken, [email protected]; J.C. Frisvad, [email protected] Abstract: Penicillium is a diverse genus occurring worldwide and its species play important roles as decomposers of organic materials and cause destructive rots in the food industry where they produce a wide range of mycotoxins. Other species are considered enzyme factories or are common indoor air allergens. Although DNA sequences are essential for robust identification of Penicillium species, there is currently no comprehensive, verified reference database for the genus. To coincide with the move to one fungus one name in the International Code of Nomenclature for algae, fungi and plants, the generic concept of Penicillium was re-defined to accommodate species from other genera, such as Chromocleista, Eladia, Eupenicillium, Torulomyces and Thysanophora, which together comprise a large monophyletic clade. -
EFFECTS of BOTANICALS and BIOCONTROL AGENTS on GROWTH and AFLATOXIN PRODUCTION by Aspergillus Flavus INFECTING MAIZE in SOME PARTS of NIGERIA
EFFECTS OF BOTANICALS AND BIOCONTROL AGENTS ON GROWTH AND AFLATOXIN PRODUCTION BY Aspergillus flavus INFECTING MAIZE IN SOME PARTS OF NIGERIA BY OKECHI, OGECHUKWU CALISTA PG/Ph.D./09/54408 DEPARTMENT OF MEDICAL LABORATORY SCIENCES FACULTY OF HEALTH SCIENCES AND TECHNOLOGY COLLEGE OF MEDICINE UNIVERSITY OF NIGERIA ENUGU CAMPUS OCTOBER, 2014 TITLE PAGE EFFECTS OF BOTANICALS AND BIOCONTROL AGENTS ON GROWTH AND AFLATOXIN PRODUCTION BY Aspergillus flavus INFECTING MAIZE IN SOME PARTS OF NIGERIA BY OKECHI, OGECHUKWU CALISTA PG/Ph.D./09/54408 A THESIS SUBMITTED TO THE DEPARTMENT OF MEDICAL LABORATORY SCIENCES, FACULTY OF HEALTH SCIENCES AND TECHNOLOGY, IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF THE DEGREE OF DOCTOR OF PHILOSOPHY (Ph.D.) IN MEDICAL LABORATORY SCIENCES (MEDICAL MIROBIOLOGY), COLLEGE OF MEDICINE, UNIVERSITY OF NIGERIA ENUGU CAMPUS SUPERVISOR: PROFESSOR N.F. ONYEMELUKWE OCTOBER, 2014 i DEPARTMENT OF MEDICAL LABORATORY SCIENCES COLLEGE OF MEDICINE UNIVERSITY OF NIGERIA Telegrams NIGERSITY, ENUGU ENUGU CAMPUS HEAD OF DEPARTMENT NIGERIA OUR REF:……………………UN/CM/MLS/B2 Tel. YOUR REF: ……………… DATE: …………… CERTIFICATION Mr / Mrs / Miss OKECHI OGECHUKWU CALISTA a Ph.D student of the Department of Medical Laboratory Sciences, College of Medicine University of Nigeria, Enugu Campus, majoring in MEDICAL MICROBIOLOGY has satisfactorily completed the requirement for the research work. The results embodied in the work have not been submitted in part or full to any Diploma or Degree of this in any other University. Supervisor’s Name: PROF N .F. ONYEMELUKWE Signature: _____________________________________ ii DEDICATION To Almighty God and my loving mother Mrs. Caroline Nwamaka Okechi. iii ACKNOWLEDGEMENTS My profound gratitude goes to Almighty God for making this study a reality.