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Catalog 2012-2013 TABLE of CONTENTS Molecular Cloning Laboratories Catalog 2012-2013 TABLE OF CONTENTS 04 Cloning 168 Competent E. coli ABOUT US Established in 1998, Molecular Cloning Laboratories (MCLAB) is a leader in providing genomic research consumables and services to the Life Sciences community. MCLAB provides cost-effective consumables and reagents, focusing on first generation as well as next-generation sequencing and DNA fragment analysis. We PCR & Real-time PCR Antibody-related Products carry a large selection of molecular biology related products, including antibodies, 16 178 biochemical reagents, cloning kits, enzymes, PCR kits, protein gels & solutions and more. Being our own manufacturer allows us to control the production process and minimizes delays in custom and large-scale orders. It helps to ensure our customers are getting the highest quality and value. DNA Sequencing, Fragment Analysis 40 and Next-Generation Sequencing 188 Nucleic Acid Purification With a team of over twenty experienced scientists, MCLAB is committed to offering first-rate services to researchers. We specialize in the field of genomics, with experts in DNA sequencing, fermentation, fragment analysis, molecular cloning, over- expression, protein purification, and protein synthesis. We take a tailored approach DNA/RNA Modifying Enzymes & to customer service, giving you the opportunity to customize your project and speak Other Proteins RNAi, MicroRNA & Gene Regulation directly with our team of scientists for technical support. With over six decades of 70 200 experience, our talented and skilled staff is dedicated to getting the results you need. MCLAB has offices in China and distributors all around the world, including Australia, Austria, Belgium, Canada, Czech Republic, Denmark, Germany, Hungary, Greece, India, Israel, Poland, New Zealand, Turkey, United Kingdom and Vietnam, so we can 132 Restriction Endonucleases 208 Bio Chemical Reagents better reach our customers. If your country is not on this list, please contact our USA headquarters for more information. 140 Protein Expression and Purification 220 Services 162 Electrophoresis Products 252 Index/Legal Reference Helpful Information Cloning Primer Sets Chaperon Proteins and Disullfide Bond Isomerase Quik-Stain ------------------------------------------------------------------- 144 Exo-Resistant Random Primer ------------------------------------------- 56 DnaK (HSP70) E. coli Recombinant ------------------------------------ 98 SDS High Molecular Weight Running Buffer ----------------------- 145 Cloning & Subcloning Primers for Sequencing ----------------------------------------- 57, 58, 59 GroEL -------------------------------------------------------------------------- 98 SDS Low Molecular Weight Running Buffer ------------------------ 145 Choo-Choo CloningTM Kits --------------------------------------- 4, 5 GroES -------------------------------------------------------------------------- 98 EZ-TOPO PCR Cloning Kits ----------------------------------------------- 6 Next-Generation Sequencing PDI1 (yeast) ------------------------------------------------------------------ 98 Protein Purification Resins RNA-Seq Library Construction Kit -------------------------------------- 60 Heparin Agarose Beads ------------------------------------------------- 146 Agar plates for E. coli Non-Amplification DNA Library Construction ---------------------- 61 Reverse Transcriptase and RNA Polymerases Glutathione Agarose Beads -------------------------------------------- 147 LB Agar Plates ------------------------------------------------------------- 7, 8 DNA dA-Tailing Kit --------------------------------------------------------- 62 Universal Reverse Transcriptase ---------------------------------------- 99 Ni-NTA Agarose ----------------------------------------------------------- 148 Super Broth Agar Plates ---------------------------------------------------- 9 DNA Ligation Kit ----------------------------------------------------------- 63 Thermostable Reverse Transcriptase -------------------------------- 100 Q Sepharose High Performance -------------------------------------- 149 Terrific Broth Agar Plates --------------------------------------------------- 9 Fragment DNA End Repair Kit ------------------------------------------ 64 Poly(A) Polymerase, E. coli --------------------------------------------- 101 SP Sepharose Big Beads ------------------------------------------------ 150 YT Agar Plates --------------------------------------------------------------- 10 DNA Storage Buffer (Low Concentration) ---------------------------- 65 Poly(A) Polymerase, Yeast ---------------------------------------------- 102 ATP Sulfurylase Yeast ------------------------------------------------------ 66 T7 RNA Polymerase ------------------------------------------------------ 103 Chaperon Proteins and Disullfide Bond Isomerase Markers Bst DNA Polymerase (large fragment) -------------------------------- 66 DnaK (HSP70) E. coli Recombinant ---------------------------------- 151 1 Kb DNA Ladder ---------------------------------------------------------- 11 Bst DNA Polymerase (regular) ------------------------------------------- 66 Kinases GroEL ------------------------------------------------------------------------ 152 100 bp DNA Ladder ------------------------------------------------------- 12 Pfu DNA Polymerase ------------------------------------------------------ 66 Pyruvate Kinase I (pykF) ------------------------------------------------- 104 GroES ------------------------------------------------------------------------ 153 Taq DNA Polyymerase (regular) ---------------------------------------- 66 T4 Polynucleotide Kinase ----------------------------------------------- 105 PDI1 (yeast) ----------------------------------------------------------------- 154 Taq DNA Polymerase (Klenow Fragment) ---------------------------- 66 Taq DNA Polymerase (truncated and exo-) -------------------------- 66 DNA Repair Proteins Protease PCR & Real-time PCR Poly(A) Polymerase, Yeast ------------------------------------------------ 66 APE 1 ------------------------------------------------------------------------- 106 SUMO Protease ----------------------------------------------------------- 155 PCR Kits Poly(A) Polymerase, E. coli ----------------------------------------------- 66 Endonuclease IV, E. coli ------------------------------------------------- 107 TEV Protease --------------------------------------------------------------- 156 2x HiFi HTP PCR Master Mix -------------------------------------------- 16 T4 DNA Ligase -------------------------------------------------------------- 66 T4 Endonuclease V ------------------------------------------------------- 108 TurboTEV Protease ------------------------------------------------------- 157 2x HotStart PCR Master Mix --------------------------------------------- 17 T4 RNA Ligase 1 (ssRNA Ligase) --------------------------------------- 66 T4 Endonuclease VII ----------------------------------------------------- 109 2x Universal Taq Master Mix ---------------------------------------- 18, 19 T4 RNA Ligase 2 (dsRNA Ligase) --------------------------------------- 66 Topoisomerase I (Vaccinia) --------------------------------------------- 110 Protein Purification solutions T4 RNA Ligase 2 (truncated) (RNL2) ----------------------------------- 66 50% IGEPAL CA-630 (NP-40) ------------------------------------------ 158 PCR Reagents Universal Reverse Transcriptase ---------------------------------------- 66 Nucleases 50% Triton x-114 ---------------------------------------------------------- 158 High Purity Solution dNTPs ---------------------------------------------- 20 Thermostable Reverse Transcriptase----------------------------------- 66 Exonuclease I (E. coli) ---------------------------------------------------- 111 7M Guanidine HCL Solution ------------------------------------------- 158 HoTaq DNA Polymerase (hot start) ------------------------------------ 21 T4 Polynucleotide Kinase ------------------------------------------------ 66 Exonuclease III (E. coli) -------------------------------------------------- 112 Ammonium Sulfate ------------------------------------------------------- 158 Pfu DNA Polymerase ------------------------------------------------------ 21 Firefly luciferase (photinus pyralis) ------------------------------------- 66 FEN1 (AFU) ----------------------------------------------------------------- 113 Antifoam 204 --------------------------------------------------------------- 158 Taq DNA Polymerase (regular) ------------------------------------------ 21 RNase Inhibitor -------------------------------------------------------------- 66 Lambda Exonuclease ---------------------------------------------------- 114 Brij-35 (30% Solution) ---------------------------------------------------- 158 Taq DNA Polymerase (exo+ and polymerase-) --------------------- 21 T7 Exonuclease ------------------------------------------------------------ 115 CHAPS ----------------------------------------------------------------------- 158 Taq DNA Polymerase (full length exo-) ------------------------------- 21 CHAPSO -------------------------------------------------------------------- 158 Taq DNA Polymerase (Klenow Fragment) ---------------------------- 21 DNA/RNA Modifying Enzymes & Other RNases TWEEN 20 ------------------------------------------------------------------ 158 Taq DNA Polymerase (truncated and exo-) -------------------------- 21 RNase H, E. coli ----------------------------------------------------------- 116 TWEEN
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