• Abstract and Figures
• Public Full-text

Potassium Channels Blockers from the Venom of Androctonus mauretanicus mauretanicus Marie-France Martin-Eauclaire, Pierre E Bougis To cite this version: Marie-France Martin-Eauclaire, Pierre E Bougis. Potassium Channels Blockers from the Venom of Androctonus mauretanicus mauretanicus. Journal of toxicology, Hindawi, 2012, 2012, pp.103608. 10.1155/2012/103608. hal-00709677 HAL Id: hal-00709677 https://hal.archives-ouvertes.fr/hal-00709677 Submitted on 15 Feb 2018 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Distributed under a Creative Commons Attribution| 4.0 International License Hindawi Publishing Corporation Journal of Toxicology Volume 2012, Article ID 103608, 9 pages doi:10.1155/2012/103608 Review Article Potassium Channels Blockers from the Venom of Androctonus mauretanicus mauretanicus Marie-France Martin-Eauclaire and Pierre E. Bougis Aix-Marseille University, CNRS, UMR 7286, CRN2M, Facult´edeM´edecine secteur Nord, CS80011, Boulevard Pierre Dramard, 13344 Marseille Cedex 15, France Correspondence should be addressed to Marie-France Martin-Eauclaire, [email protected] and Pierre E. Bougis, [email protected] Received 2 February 2012; Accepted 16 March 2012 Academic Editor: Maria Elena de Lima Copyright © 2012 M.-F. Martin-Eauclaire and P. E. Bougis. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. K+ channels selectively transport K+ ions across cell membranes and play a key role in regulating the physiology of excitable and nonexcitable cells. Their activation allows the cell to repolarize after action potential firing and reduces excitability, whereas channel inhibition increases excitability. In eukaryotes, the pharmacology and pore topology of several structural classes of K+ channels have been well characterized in the past two decades. This information has come about through the extensive use of scorpion toxins. We have participated in the isolation and in the characterization of several structurally distinct families of scorpion toxin peptides exhibiting different K+ channel blocking functions. In particular, the venom from the Moroccan scorpion Androctonus ffi + + mauretanicus mauretanicus provided several high-a nity blockers selective for diverse K channels (SKCa,Kv4.x, and Kv1.x K channel families). In this paper, we summarize our work on these toxin/channel interactions. 1. The Scorpion Venom Content to extracellular loops located preferentially on the voltage sensor of the Nav channel domain II (but also occasionally Scorpion venoms are very complex mixtures of molecules, of domain III) [1, 3–6]. constituting a diverse, naturally occurring peptide library, Another class of scorpion toxins has also been widely with most peptides displaying different kinds of biological studied, even if these toxins are devoid of serious lethal effect. activity [1, 2]. These peptides can specifically bind to a vari- They block different K+ channel subtypes (some of them in ety of pharmacological targets, in particular ion channels, the picomolar range) and are so-called “K+ channel toxins” ff + + resulting in neurotoxic e ects. Toxins modulating Na ,K , [1, 2, 7]. They are usually shorter than Nav channel toxins ++ − Ca ,andCl currents have been described in scorpion but are structurally closely related to them. These Nav and venoms [2]. K+ channels toxins share a common dense scaffold typically Toxins that are highly lethal for mammals modify vol- formed by an α-helix and a β-sheet stabilized by disulfide + tage-gated Na (Nav) currents in excitable cells and are bridges [8]. − referred to as “Nav channel long-chain toxin.” These toxins Several components of scorpion venom that act on Cl are single-chain, small, basic peptides (60- to 75-amino-acid and Ca++ channels have also been described [2]. However, residue chain generally folded by four disulfide bridges). they have little or no influence on the venom toxicity for They have been described as α-orβ-toxins due to their mammals. binding site on Nav channels as well as to their pharmaco- This overview will focus, in particular, on our work logical effects [1, 3]. α-Toxins bind in a voltage-dependent done on the K+ channel blockers purified from the Androc- manner on the voltage sensor of the Nav channel domain tonus mauretanicus mauretanicus venom, which were among IV and inhibit the inactivation phase of the action potential. the first K+ channel blockers characterized from scorpion β-Toxins act on the channel activation phase by binding venoms. 2 Journal of Toxicology 2. K+ Channel Blockers from Scorpion Venoms been characterized and classified as the β-KTx family. They possess two structural and functional domains: an N- + K channels constitute a ubiquitous family of transmem- terminal α-helix (with cytolytic or antimicrobial activity brane proteins which play a key role in the regulation like the insect defensins) and a tightly folded C-terminal of a wide variety of physiological processes involved in region with the CSH motif (displaying K+ channel-blocking cell excitability, including regulation of heart beat, muscle activities [15]). Finally, the γ-KTx family was described as contraction, neurotransmitter release, hormonal secretion, specifically targeting hERG channels [16]. signal transduction, and cell proliferation [9]. Multiple combinations of K+ channels result from the ability of 3. The Androctonus mauretanicus their subunits to coassemble as tetramers, thus considerably mauretanicus Venom increasing the total number of functionally distinct K+ chan- + nels. According to their functional and gating properties, K Scorpion stings in Morocco are the primary cause of channels have been first divided into four groups: voltage- envenomation and constitute a largely underestimated health 2+ + activated, Ca -activated, inward rectifier, and two-pore K problem. An epidemiologic study of four regions of the channels [10]. Their 3D architecture has now been depicted Moroccan Kingdom, where scorpion stings are prevalent, by X-ray crystallography [11]. showed that the stings are mainly due to the black scor- + K channel blocker’s toxins (KTxs) from scorpion ven- pion Androctonus mauretanicus mauretanicus (83% of the oms are short peptides, which are made usually of about reported cases). Children, in desert areas far from medical 28–40-amino-acid residues reticulated by three or four centers, were the primary victims, with casualty rates up disulfide bridges, forming compact and resistant molecules to 8% in those under ten years old. The Androctonus [7]. They have been invaluable tools for understanding the mauretanicus mauretanicus venom is one of the most toxic + physiological role of K channels and have been exploited Buthidae venoms ever described (its median lethal dose to gain insights into the structure of the channel pore that ranges from 0.05 to 0.2 mg/kg by subcutaneous injection in they occlude via electrostatic and hydrophobic interactions mice) and immunotherapy remains the treatment of choice + [12, 13]. They block K channels from the extracellular [17]. side and bind to their outer vestibules. In most cases, they Previous fractionation studies of the venom allowed possess at least two functionally crucial residues: examples identification of several toxins that are active on different include a lysine residue that plugs the channel pore with its Nav or Kv channels [18–22]. At least several major proteins, side chain and a hydrophobic residue that strengthens the considered highly toxic to mice, have already been purified interaction between the toxin and its target. These residues and chemically and pharmacologically characterized as clas- are found in very low concentrations in the venoms (from sical α-toxins [18, 19]. All together, these toxins represent 0.01 to 1% by weight of crude venom) and have almost no about 28% of the absorbance (wavelength 280 nm) of the toxic effects in mice when injected by subcutaneous route. crude venom and 73% of the total lethality for mice. The However, some of them could be very toxic following direct most represented and the most lethal classical α-toxin Amm intracerebroventricular injection. V alone constitutes 11% of the absorbance and 47% of the Based on primary amino acid sequences and cysteine total lethality. pairing, KTxs have been classified into four families, the α-, However, other toxins isolated from the Androctonus β-, γ-, and κ-KTx [2, 7]. So far more than 120 KTxs, ranging mauretanicus mauretanicus venom gained popularity as from 23 to 64 amino acids, have been isolated and sequenced. powerful tools because they have displayed some of the Most of their structures exhibit a common minimal motif, highest binding affinity and specificity for K+ channels. They named the “Cystein-Stabilized-Helix” (CSH). As found in have been extensively used to investigate the mechanisms Buthidae scorpions, long and short toxins consist of one of ion conduction

Load more

  11

Copy Link