(CYP97C) from the Green Alga Haematococcus Pluvialis
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Efficient Microscale Screening of Various Haematococcus Pluvialis Strains for Growth and Astaxanthin Production
Efficient microscale screening of various Haematococcus pluvialis strains for growth and astaxanthin production Inaugural-Dissertation zur Erlangung des Doktorgrades der Mathematisch-Naturwissenschaftlichen Fakultät der Universität zu Köln vorgelegt von Zehra Çebi aus Köln Köln, 2017 Berichterstatter: Prof. Dr. Michael Melkonian (Gutachter) Prof Dr. Burkhard Becker Tag der mündlichen Prüfung: 23.01.2017 3 Zusammenfassung Das Ketocarotenoid Astaxanthin wird in der Natur von einigen Algen, Pflanzen, Pilzen und Bakterien synthetisiert. Hierbei besitzt die Grünalge Haematococcus pluvialis mit bis zu 4% des Trockengewichtes die höchste Kapazität Astaxanthin zu akkumulieren. Kommerziell wird natürliches Astaxanthin aus H. pluvialis als pharmazeutisch-funktionelles Lebensmittel für den Menschen und hauptsächlich als Färbemittel in der Aquakultur verwendet. Aufgrund hoher Produktionskosten von natürlichem Astaxanthin aus H. pluvialis wird der kommerzielle Astaxanthinmarkt von dem synthetischen Analogon dominiert. Da jedoch die Nachfrage für natürliches Astaxanthin stetig steigt, laufen die Bestrebungen zur Verbesserung von Massenkultursystemen für H. pluvialis, insbesondere auf technischer Ebene, auf Hochtouren, um die Produktionskosten zu senken und damit die Konkurrenzfähigkeit von natürlichem Astaxanthin auf dem Carotenoidmarkt zu erhöhen. Der Fokus dieser Doktorarbeit liegt auf der Verbesserung der H. pluvialis Produktivität auf biologischer Ebene, nämlich durch Selektion und genetische Manipulation eines effizienten H. pluvialis Stammes. -
Neoproterozoic Origin and Multiple Transitions to Macroscopic Growth in Green Seaweeds
Neoproterozoic origin and multiple transitions to macroscopic growth in green seaweeds Andrea Del Cortonaa,b,c,d,1, Christopher J. Jacksone, François Bucchinib,c, Michiel Van Belb,c, Sofie D’hondta, f g h i,j,k e Pavel Skaloud , Charles F. Delwiche , Andrew H. Knoll , John A. Raven , Heroen Verbruggen , Klaas Vandepoeleb,c,d,1,2, Olivier De Clercka,1,2, and Frederik Leliaerta,l,1,2 aDepartment of Biology, Phycology Research Group, Ghent University, 9000 Ghent, Belgium; bDepartment of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Zwijnaarde, Belgium; cVlaams Instituut voor Biotechnologie Center for Plant Systems Biology, 9052 Zwijnaarde, Belgium; dBioinformatics Institute Ghent, Ghent University, 9052 Zwijnaarde, Belgium; eSchool of Biosciences, University of Melbourne, Melbourne, VIC 3010, Australia; fDepartment of Botany, Faculty of Science, Charles University, CZ-12800 Prague 2, Czech Republic; gDepartment of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742; hDepartment of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138; iDivision of Plant Sciences, University of Dundee at the James Hutton Institute, Dundee DD2 5DA, United Kingdom; jSchool of Biological Sciences, University of Western Australia, WA 6009, Australia; kClimate Change Cluster, University of Technology, Ultimo, NSW 2006, Australia; and lMeise Botanic Garden, 1860 Meise, Belgium Edited by Pamela S. Soltis, University of Florida, Gainesville, FL, and approved December 13, 2019 (received for review June 11, 2019) The Neoproterozoic Era records the transition from a largely clear interpretation of how many times and when green seaweeds bacterial to a predominantly eukaryotic phototrophic world, creat- emerged from unicellular ancestors (8). ing the foundation for the complex benthic ecosystems that have There is general consensus that an early split in the evolution sustained Metazoa from the Ediacaran Period onward. -
The Symbiotic Green Algae, Oophila (Chlamydomonadales
University of Connecticut OpenCommons@UConn Master's Theses University of Connecticut Graduate School 12-16-2016 The yS mbiotic Green Algae, Oophila (Chlamydomonadales, Chlorophyceae): A Heterotrophic Growth Study and Taxonomic History Nikolaus Schultz University of Connecticut - Storrs, [email protected] Recommended Citation Schultz, Nikolaus, "The yS mbiotic Green Algae, Oophila (Chlamydomonadales, Chlorophyceae): A Heterotrophic Growth Study and Taxonomic History" (2016). Master's Theses. 1035. https://opencommons.uconn.edu/gs_theses/1035 This work is brought to you for free and open access by the University of Connecticut Graduate School at OpenCommons@UConn. It has been accepted for inclusion in Master's Theses by an authorized administrator of OpenCommons@UConn. For more information, please contact [email protected]. The Symbiotic Green Algae, Oophila (Chlamydomonadales, Chlorophyceae): A Heterotrophic Growth Study and Taxonomic History Nikolaus Eduard Schultz B.A., Trinity College, 2014 A Thesis Submitted in Partial Fulfillment of the Requirements for the Degree of Master of Science at the University of Connecticut 2016 Copyright by Nikolaus Eduard Schultz 2016 ii ACKNOWLEDGEMENTS This thesis was made possible through the guidance, teachings and support of numerous individuals in my life. First and foremost, Louise Lewis deserves recognition for her tremendous efforts in making this work possible. She has performed pioneering work on this algal system and is one of the preeminent phycologists of our time. She has spent hundreds of hours of her time mentoring and teaching me invaluable skills. For this and so much more, I am very appreciative and humbled to have worked with her. Thank you Louise! To my committee members, Kurt Schwenk and David Wagner, thank you for your mentorship and guidance. -
Sammy Boussiba December 2013
Sammy Boussiba December 2013 CURRICULUM VITAE AND LIST OF PUBLICATIONS Personal Details Name: Sammy Boussiba Date and Place of Birth: August 30, 1947, Fes, Morocco Date of Immigration: 1956 Regular Military Service: 8/1966-9/69 Address at Work: Microalgal Biotechnology Laboratory Jacob Blaustein Desert Research Institute Ben-Gurion University of the Negev Sede-Boker Campus 84990, Israel Tel: 972-8- 6596795, 6596796 Address at Home: Ya'ara 16, Omer , 84965 Israel Education B.Sc. - 1969-72 - Hebrew University of Jerusalem and Ben-Gurion University of the Negev. M.Sc. - 1973-75 - Hebrew University of Jerusalem and Ben-Gurion University of the Negev. Advisor - Prof. A. Richmond. Title of thesis: The Involvement of Abscisic Acid in the Interrelationships between Various Stresses and Recovery. Ph.D. - 1976-81 - Ben-Gurion University of the Negev. Advisor - Prof. A. Richmond. Title of thesis: The Biliprotein C-Phycocyanin: Its Possible Role and Influence of Environment Factors on Its Metabolism. Employment History A. Employment 2001 Professor, The Jacob Blaustein Institute for Desert Research, BGU. 1995 Associate Professor, The Jacob Blaustein Institute for Desert Research, BGU. 1989 Researcher Grade A, The Jacob Blaustein Institute for Desert Research, BGU. 1985-88 Senior Researcher, The Jacob Blaustein Institute for Desert Research, BGU. 1978-84 Research Fellow - The Jacob Blaustein Institute for Desert Research, BGU. 1973-77 Research Assistant. Biology Department - Ben-Gurion University of the Negev. B. Employment abroad 2005 Visiting Professor (sabbatical), Instituto Bioquimica Vegetal y Fotosintesis, Sevilla, Spain (with Prof. Miguel Guerrero) 1991-92 Visiting Res. Prof. (sabbatical), Arizona State University, Department of Botany (with Prof. -
4 – RESULTS & Discussion
UNIVERSIDADE DE LISBOA FACULDADE DE CIÊNCIAS DEPARTAMENTO DE BIOLOGIA VEGETAL Towards improvement of Haematococcus pluvialis cultures by cell sorting and UV mutagenesis Filipa Faria Rosa Mestrado em Microbiologia Aplicada Dissertação orientada por: Doutor Luís Tiago Guerra Professora Doutora Ana Tenreiro 2017 Towards improvement of Haematococcus pluvialis cultures by cell sorting and UV mutagenesis Filipa Faria Rosa 2017 This thesis was fully performed at A4F – Algae for Future and Bugworkers Laboratory | M&B – BioISI | Teclabs under the direct supervision of Doutor Luís Tiago Guerra. Professora Doutora Ana Tenreiro was the internal supervisor in the scope of the Master in Applied Microbiology of the Faculty of Sciences of the University of Lisbon. 4 – RESULTS & DISCUSSION 4 – RESULTS & DISCUSSION ACKNOWLEDGMENTS First of all, I would like to show my gratitude to the administration of A4F – Alga for Future. Not only they gave me the opportunity of carrying out my master thesis but also I was able to work in two laboratories, A4F and Bugworkers Laboratory | M&B – BioISI | Teclabs, thanks to their partnership. It was an exceptional and exclusive experience which I will never forget. I want to thank Dr. Luis Tiago Guerra, my supervisor at A4F, and Professora Doutora Ana Tenreiro, my supervisor at Bugworkers Laboratory | M&B – BioISI | Teclabs. I am especially grateful for all the knowledge and guidance given along the entire thesis, as well as the availability they showed to help whenever I needed. Not least, thank for the advices, patience, trust and other contributions that allowed the improvement of this work. I am thankful to all my A4F’s colleagues, especially the ones from the laboratory, who gave me all their support and most important, provided me great and unforgettable moments in the laboratory. -
National Algal Biofuels Technology Roadmap
BIOMASS PROGRAM National Algal Biofuels Technology Roadmap MAY 2010 National Algal Biofuels Technology Roadmap A technology roadmap resulting from the National Algal Biofuels Workshop December 9-10, 2008 College Park, Maryland Workshop and Roadmap sponsored by the U.S. Department of Energy Office of Energy Efficiency and Renewable Energy Office of the Biomass Program Publication Date: May 2010 John Ferrell Valerie Sarisky-Reed Office of Energy Efficiency Office of Energy Efficiency and Renewable Energy and Renewable Energy Office of the Biomass Program Office of the Biomass Program (202)586-5340 (202)586-5340 [email protected] [email protected] Roadmap Editors: Daniel Fishman,1 Rajita Majumdar,1 Joanne Morello,2 Ron Pate,3 and Joyce Yang2 Workshop Organizers: Al Darzins,4 Grant Heffelfinger,3 Ron Pate,3 Leslie Pezzullo,2 Phil Pienkos,4 Kathy Roach,5 Valerie Sarisky-Reed,2 and the Oak Ridge Institute for Science and Education (ORISE) A complete list of workshop participants and roadmap contributors is available in the appendix. Suggested Citation for This Roadmap: U.S. DOE 2010. National Algal Biofuels Technology Roadmap. U.S. Department of Energy, Office of Energy Efficiency and Renewable Energy, Biomass Program. Visit http://biomass.energy.gov for more information 1BCS, Incorporated 2Office of the Biomass Program 3Sandia National Laboratories 4National Renewable Energy Laboratory 5MurphyTate LLC This report is being disseminated by the Department of Energy. As such, the document was prepared in compliance with Section 515 of the Treasury and General Government Appropriations Act for Fiscal Year 2001 (Public Law No. 106-554) and information quality guidelines issued by the Department of energy. -
Morphological, Molecular, and Biochemical Characterization of Astaxanthin-Producing Green Microalga Haematococcus Sp
J. Microbiol. Biotechnol. (2015), 25(2), 238–246 http://dx.doi.org/10.4014/jmb.1410.10032 Research Article Review jmb Morphological, Molecular, and Biochemical Characterization of Astaxanthin-Producing Green Microalga Haematococcus sp. KORDI03 (Haematococcaceae, Chlorophyta) Isolated from Korea Ji Hyung Kim1†, Md. Abu Affan1,2†, Jiyi Jang1, Mee-Hye Kang3, Ah-Ra Ko4, Seon-Mi Jeon1, Chulhong Oh1, Soo-Jin Heo1, Youn-Ho Lee3, Se-Jong Ju4, and Do-Hyung Kang1* 1Global Bioresources Research Center, Korea Institute of Ocean Science & Technology, Seoul 426-744, Republic of Korea 2Department of Marine Biology, Faculty of Marine Science, King AbdulAziz University, Jeddah 21589, Saudi Arabia 3Marine Ecosystem Research Division, Korea Institute of Ocean Science & Technology, Seoul 426-744, Republic of Korea 4Deep-sea and Seabed Resources Research Division, Korea Institute of Ocean Science & Technology, Seoul 426-744, Republic of Korea Received: October 15, 2014 Revised: November 4, 2014 A unicellular red microalga was isolated from environmental freshwater in Korea, and its Accepted: November 4, 2014 morphological, molecular, and biochemical properties were characterized. Morphological analysis revealed that the isolate was a unicellular biflagellated green microalga that formed a non-motile, thick-walled palmelloid or red aplanospore. To determine the taxonomical First published online position of the isolate, its 18S rRNA and rbcL genes were sequenced and phylogenetic analysis November 10, 2014 was performed. We found that the isolate was clustered together with other related *Corresponding author Haematococcus strains showing differences in the rbcL gene. Therefore, the isolated microalga Phone: +82-31-400-7733; was classified into the genus Haematococcus, and finally designated Haematococcus sp. -
Evolutionary Origins, Molecular Cloning And
Cui et al. BMC Genomics 2013, 14:457 http://www.biomedcentral.com/1471-2164/14/457 RESEARCH ARTICLE Open Access Evolutionary origins, molecular cloning and expression of carotenoid hydroxylases in eukaryotic photosynthetic algae Hongli Cui1,2†, Xiaona Yu3†, Yan Wang1, Yulin Cui1, Xueqin Li4, Zhaopu Liu3 and Song Qin1* Abstract Background: Xanthophylls, oxygenated derivatives of carotenes, play critical roles in photosynthetic apparatus of cyanobacteria, algae, and higher plants. Although the xanthophylls biosynthetic pathway of algae is largely unknown, it is of particular interest because they have a very complicated evolutionary history. Carotenoid hydroxylase (CHY) is an important protein that plays essential roles in xanthophylls biosynthesis. With the availability of 18 sequenced algal genomes, we performed a comprehensive comparative analysis of chy genes and explored their distribution, structure, evolution, origins, and expression. Results: Overall 60 putative chy genes were identified and classified into two major subfamilies (bch and cyp97) according to their domain structures. Genes in the bch subfamily were found in 10 green algae and 1 red alga, but absent in other algae. In the phylogenetic tree, bch genes of green algae and higher plants share a common ancestor and are of non-cyanobacterial origin, whereas that of red algae is of cyanobacteria. The homologs of cyp97a/c genes were widespread only in green algae, while cyp97b paralogs were seen in most of algae. Phylogenetic analysis on cyp97 genes supported the hypothesis that cyp97b is an ancient gene originated before the formation of extant algal groups. The cyp97a gene is more closely related to cyp97c in evolution than to cyp97b. -
Culturing a Novel Strain of Haematococcus Pluvialis, LSBB612 (ALG App006)
Culturing a novel strain of Haematococcus pluvialis, LSBB612 (ALG_App006) Background The genus Haematococcus is found globally, with reports of isolates from all continents with the exception of Antarctica, with hostile areas of isolation including the artic circle (Klochkova et al., 2013). H. pluvialis is of commercial interest due to its ability to produce copious amounts of astaxanthin, reaching up to 5 % dry weight in the encysted aplanospore state (Wayame et al., 2013). Astaxanthin is sold as a pigment for aquaculture and in animal feed, and is marketed as an antioxidant for the nutraceutical market. The H. pluvialis derived astaxanthin industry is commercially successful; however, several constraints are ever-present including issues of contamination and grazing, high extraction costs, high light requirements for encystment, and conversely, photo-bleaching (Shah et al., 2016). Astaxanthin is produced under high light and nutrient deplete conditions (García-Malea et al., 2008). High temperature is rarely implemented to induce astaxanthin production, as it was reported to severely reduce biomass yield, and thus decrease astaxanthin productivity (Tjahjono et al. 1994). Currently the red stage of astaxanthin production is constrained by biomass production in the green stage, which requires strictly controlled culture conditions. Optimal reported temperatures for the vegetative growth of H. pluvialis are between 20 and 28°C (Wan et al., 2014), with temperatures in excess of 30°C shown to induce transition from the green vegetative stage to the red stage with the formation of aplanospores. Domínguez-Bocanegra et al., (2004) demonstrated optimal growth at an irradiance of 177 µmol photons/m 2/s with higher density cultures achieved under continuous light. -
Environmental Stewardship by Microalgae: Air and Water Juhyon Kang Iowa State University
Iowa State University Capstones, Theses and Graduate Theses and Dissertations Dissertations 2015 Environmental stewardship by microalgae: air and water Juhyon Kang Iowa State University Follow this and additional works at: https://lib.dr.iastate.edu/etd Part of the Environmental Engineering Commons, and the Natural Resources Management and Policy Commons Recommended Citation Kang, Juhyon, "Environmental stewardship by microalgae: air and water" (2015). Graduate Theses and Dissertations. 14570. https://lib.dr.iastate.edu/etd/14570 This Dissertation is brought to you for free and open access by the Iowa State University Capstones, Theses and Dissertations at Iowa State University Digital Repository. It has been accepted for inclusion in Graduate Theses and Dissertations by an authorized administrator of Iowa State University Digital Repository. For more information, please contact [email protected]. Environmental stewardship by microalgae - air and water by Juhyon Kang A dissertation submitted to the graduate faculty in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY Co-majors: Food Science and Technology; Biorenewable Resources & Technology Program of Study Committee: Zhiyou Wen, Major Professor Tong Wang Byron F. Brehm-Stecher Hongwei Xin Jacek A. Koziel Iowa State University Ames, Iowa 2015 Copyright © Juhyon Kang, 2015. All rights reserved. ii TABLE OF CONTENTS Page ACKNOWLDEGEMENTS ............................................................................................................ v ABSTRACT -
Sources of Inorganic Fertilizer in the Growth of Haematococcus Pluvialis Flotow (Chlorophyceae)
J. Algal Biomass Utln. 2017, 8(2): 1-10 Sources inorganic in the growth of H. pluvialis. eISSN: 2229 – 6905 Sources of inorganic fertilizer in the growth of Haematococcus pluvialis Flotow (Chlorophyceae). 1Bruno Scardoelli-Truzzi and Lucia Helena Sipaúba-Tavares Aquaculture Center, Univ. Estadual Paulista-UNESP, Limnology and Plankton Production Laboratory, 14884-900 Jaboticabal SP Brazil. 1Corresponding author: Tel:+55 1632032110 ; fax: +55 16 32032268; E-mail address: [email protected] Abstract The influence of inorganic fertilizer media at different concentrations on the growth and biochemical composition of Haematococcus pluvialis microalgae was evaluated. Microalgae were placed in a bath culture with inorganic fertilizer (NPK) medium and WC medium during 28 days, Growth and biological aspects of microalgae between the media were compared. Mineral concentrations affected the physiological and growth rate of H. pluvialis, and the balance of nitrogen and phosphorus concentration favored higher algal biomass. High concentration of protein occurred when concentrations of nitrogen and phosphorus occurred in cultures NPK 20:5:20 and NPK 4:14:8. Amino acid profile were significantly higher (p<0.05) in culture media based on inorganic fertilizer when compared to that in commercial medium (WC). High cell density (4.6 x 105 cells.mL-1) was obtained in NPK 10:10:10 and in WC medium (3.2 x 105 cells.mL-1) after 16 days cultivation. Media inorganic fertilizer (NPK) was adequate and may replace the commercial medium WC for the cultivation of microalgae H. pluvialis, with high nutritional value and biomass concentration. Keywords: NPK and WC media; biological aspects, nutritional value Introduction Nutrients are greatly relevant in the growth and development of microalgae. -
AOAC Compiled Strain List 14
AOACC Compiled Strain List 14 Sept 2011 Collection Strain number Other strain number Taxonomy Scientific name Synonyms Former name Source Country Source Locality Source type (freshwater, Date of Date of Isolator History of deposit Culture Growth Light Other Status Type or Toxicity Restrictions Remarks (metabolites, marine, etc.) collection isolation (Depositor) medium temperat intensity preservation authentic on characteristics, etc.) (DD/MM/YYYY (DD/MM/YYYY ure (ºC) (umol (cryopreservatio strain distribution/ or other style, or other style, photons m- n, DNA, etc.) condition of e.g. YYYY-MM- e.g. YYYY-MM- 2 s-1) use DD ) DD ) 1 NIES NIES-2412 Muroran strain Cyanophyceae Acaryochloris marina Miyashita et Chihara Japan Hokkaido University Marine Marine 2004-06-20 2004-08-** Miyashita, Hideaki < Miyashita, Hideaki IMK 25 70-90 Subculture Unialgal Halophilic; Contains Station, Hokkaido chlorophyll d 2 NIES NIES-1349 Bacillariophyceae Achnanthes kuwaitensis Hendey Japan Morito Beach, Kanagawa Marine 2003-04-19 2003-04-22 Shono, Naoko < Mayama, Shigeki f/2 20 15-27 Subculture Unialgal; Clonal; Non-axenic 3 NIES NIES-330 IMHB-5 Bacillariophyceae Achnanthes subconstricta (Meister) Toyoda Achnanthes longipes Agardh Japan Imaihama, Shizuoka Marine 1985-05-22 1985-05-31 Sawaguchi, Tomohiro < Sawaguchi, Tomohiro f/2 10 15-20 Subculture Unialgal; Clonal; Non-axenic 4 NIES NIES-1350 Bacillariophyceae Achnanthidium minutissimum (Kützing) Czarnecki Achnanthes minutissima Kützing Japan Tama River, Tokyo Freshwater 2002-11-26 2002-11-26 Shono, Naoko <