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WO 2018/039632 Al 01 March 2018 (01.03.2018) W !P O PCT

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WO 2018/039632 Al 01 March 2018 (01.03.2018) W !P O PCT (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2018/039632 Al 01 March 2018 (01.03.2018) W !P O PCT (51) International Patent Classification: (74) Agent: CONKLIN, David R.; 36 S. State Street, Suite A23C 9/15 (2006.01) A23J 3/08 (2006.01) 1900, Salt Lake City, Utah 841 11 (US). A23C 20/00 (2006.01) A23J 3/14 (2006.01) (81) Designated States (unless otherwise indicated, for every A23C 21/08 (2006.01) kind of national protection available): AE, AG, AL, AM, (21) International Application Number: AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, PCT/US20 17/048730 CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, (22) International Filing Date: HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, 25 August 2017 (25.08.2017) KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, (25) Filing Language: English MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, (26) Publication Language: English SC, SD, SE, SG, SK, SL, SM, ST, SV, SY,TH, TJ, TM, TN, (30) Priority Data: TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. 25 August 2016 (25.08.2016) US 62/379,647 (84) Designated States (unless otherwise indicated, for every (71) Applicant: PERFECT DAY, INC. [US/US]; 813 Heinz kind of regional protection available): ARIPO (BW, GH, Ave, Berkely, California 94710 (US). GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, (72) Inventors: GEISTLINGER, Timothy; 6066 Aspinwall TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, Road, Oakland, California 9461 1 (US). JHALA, Ravira- EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, jsinh; 799 Bounty Drive Apt 104, Foster City, Califor MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, nia 94404 (US). KRUEGER, Kate Patricia; 116 Court St TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, Apt 605, New Haven, Connecticut 065 11(US). RAMESH, KM, ML, MR, NE, SN, TD, TG). Balakrishnan; 166 Winfield St., San Francisco, California 941 10 (US). (54) Title: FOOD PRODUCTS COMPRISING MILK PROTEINS AND NON-ANIMAL PROTEINS, AND METHODS OF PRO DUCING THE SAME © 00 FIG. 1 o (57) Abstract: Provided are food products comprising milk proteins and non-animal proteins, and methods of manufacturing the same. [Continued on nextpage] WO 2018/039632 Al llll II II 11 III II I llll I I II II I il II I II Published: — with international search report (Art. 21(3)) — before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) FOOD PRODUCTS COMPRISING MILK PROTEINS AND NON- ANIMAL PROTEINS, AND METHODS OF PRODUCING THE SAME FIELD OF THE INVENTION Provided are food products comprising milk proteins and non-animal proteins, and methods of manufacturing the same. BACKGROUND OF THE INVENTION Milk is a popular source of nutrition. It comprises high-quality protein, essential minerals (e.g., calcium, phosphorus, zinc, magnesium), and vitamins (e.g., riboflavin, vitamin A, vitamin B12). In addition, milk components possess advantageous functional characteristics that permit production of a wide variety of derivative dairy products, including yogurt, cheese, cream, ice cream, and butter, which further contribute to the industrial and cultural significance of milk. Global yearly sales of dairy products are over $500 billion, and projected to grow. Advantageous functional characteristics of milk components include denaturation, aggregation, and interactions of milk proteins, the type and extent of which can be influenced through processing conditions such as time, temperature, pH, protein content, ionic strength, lipid content, carbohydrate content, shear, and enzyme and starter culture action, and are largely responsible for the unique functional and sensory properties of milk and its derivative dairy products. But milk and its derivative dairy products also harbor attributes that are suboptimal for human consumption, such as, for example, comprising components that cause unhealthy reactions in humans (e.g., allergies, lactose intolerance). Such suboptimal attributes are not easily correctable as milk is a complex mixture of several thousand components, the types and amounts of which are defined by the function of the mammary gland of the animal that produced the milk, which in turn evolved over millions of years to serve the particular needs of the animal's offspring and cannot easily be altered. There is also widespread concern over the impact of conventional milk production via animal husbandry on animal welfare and the environment, and the potential danger of contaminating products derived from animal husbandry with pathogens, pesticide residues, heavy metals, and aflatoxin M l . These concerns have fueled development of alternatives to milk and its derivative dairy products, such as lactose-free dairy products and plant- or nut-based dairy-like products (e.g., soy milk, almond milk, coconut milk, pea milk). U.S. sales of lactose-free dairy products in 2015 were $6.7 billion, and U.S. sales of plant- or nut-based dairy-like products in 2015 were $13.7 billion. However, the currently available alternatives to milk fall short on matching the flavor and nutritional profiles of conventionally produced milk, and are limited in their utility for producing alternative derivative dairy products. Therefore, there exists a need for food products that have identical or superior attributes as milk and/or its derivative dairy products, and for methods for producing such food products. SUMMARY OF THE INVENTION Provided herein are food products that comprise one or more native and/or recombinant milk proteins and one or more native and/or recombinant non-animal proteins and/or hydrolyzed non-animal proteins and that has desirable attributes. In some embodiments, the food products are dairy products. In some embodiments, the food products comprise native and/or recombinant milk proteins with non-native glycosylation and/or phosphorylation patterns. In some embodiments, the food products are essentially free of animal lipids, animal carbohydrates, animal proteins other than the one or more milk proteins, allergenic epitopes, and/or lactose. Also provided herein are yoghurt-like food products that comprise one or more native and/or recombinant milk proteins and one or more native and/or recombinant non-animal proteins and/or hydrolyzed non-animal proteins. In some embodiments, the yoghurt-like food products comprise milk proteins with non-native glycosylation and/or phosphorylation patterns. In some embodiments, the yoghurt-like food products comprise a single milk protein, wherein the single milk protein is β-lactoglobulin. Also provided herein are methods for producing the food products provided herein. BRIEF DESCRIPTION OF THE FIGURES Figure 1 shows a perspective view of a yoghurt-like food product, in accordance with a representative embodiment of the present invention. Figure 2 shows pH profiles of whole milk yoghurt and yoghurt-like food products as a function of composition and duration of fermentation of milk-type cultures, in accordance with representative embodiments of the present invention. Figure 3 shows viscosity profiles of whole milk yoghurt and yoghurt-like food products as a function of composition and shear rate, in accordance with representative embodiments of the present invention. DETAILED DESCRIPTION OF THE INVENTION Unless defined otherwise, all technical and scientific terms used herein have the same meaning as is commonly understood by one of ordinary skill in the art to which this disclosure pertains. Further, unless otherwise required by context, singular terms shall include the plural, and plural terms shall include the singular. Definitions The terms "a" and "an" and "the" and similar references as used herein refer to both the singular and the plural, unless otherwise indicated herein or clearly contradicted by context. The terms "about" and "similar to" as used to herein refer to being within an acceptable error range for the particular value as determined by one of ordinary skill in the art, which can depend in part on how the value is measured or determined, or on the limitations of the measurement system. The term "dairy product" as used herein refers to milk (e.g., whole milk [at least 3.25% milk fat], partly skimmed milk [from 1% to 2% milk fat], skim milk [less than 0.2% milk fat], cooking milk, condensed milk, flavored milk, goat milk, sheep milk, dried milk, evaporated milk, milk foam), and products derived from milk, including but not limited to yogurt (e.g., whole milk yogurt [at least 6 grams of fat per cup], low-fat yogurt [between 2 and 5 grams of fat per cup], nonfat yogurt [less than 0.5 percent milk fat by weight], greek yogurt [strained yogurt with whey removed], whipped yogurt, goat milk yogurt, Labneh [labne], sheep milk yogurt, yogurt drinks [e.g., whole milk Kefir, low-fat milk Kefir], Lassi), cheese (e.g., whey cheese such as ricotta and mozzarella, semi-soft cheese such as Havarti and Munster, medium-hard cheese such as Swiss and Jarlsberg, hard cheese such as Cheddar, soft ripened cheese such as Brie and Camembert, cottage cheese, cream cheese, curd), cream (e.g., whipping cream, coffee whitener, coffee creamer, sour cream, creme fraiche), frozen confections (e.g., ice cream, smoothie, milk shake, frozen yogurt, sundae), butter, infant formula, weight loss beverages, nutritional beverages, pudding, buttermilk, milk protein concentrate, whey protein concentrate, whey protein isolate, casein concentrate, casein isolate, skim milk powder, whole milk powder, nutritional supplements, texturizing blends, flavoring blends, or coloring blends.
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