Supplemental Data Inter-Individual Variability in Gene Expression

DMD #42028

Supplemental data

Inter-individual variability in gene expression profiles in human hepatocytes and comparison with HepaRG cells

Alexandra ROGUE, Carine LAMBERT, Catherine SPIRE, Nancy CLAUDE and André GUILLOUZO

Drug and metabolism disposition

Supplemental Table 2 : Intensity values of some major genes in the 6 human hepatocyte populations and HepaRG cells.

Gene symbol Gene description Donor # 1 Donor # 2 Donor # 3 Donor # 4 Donor # 5 Donor # 6 HepaRG ABAT 4-aminobutyrate aminotransferase 14539.39 11602.69 14616.05 17618.16 4899.27 5254.34 11928.62 ABCB1 ATP-binding cassette, sub-family B (MDR/TAP), member 1 863.91 1677.95 1158.19 1235.74 2589.67 2299.44 5336.81 ABCB10 ATP-binding cassette, sub-family B (MDR/TAP), member 10 138.92 186.97 172.54 132.16 90.82 140.79 465.96 ABCB11 ATP-binding cassette, sub-family B (MDR/TAP), member 11 535.99 135.67 195.99 120.14 30.70 70.87 255.17 ABCB4 ATP-binding cassette, sub-family B (MDR/TAP), member 4 803.95 384.93 372.87 235.11 151.23 279.58 989.41 ABCB6 ATP-binding cassette, sub-family B (MDR/TAP), member 6 3833.11 4199.17 6405.20 4370.31 3918.44 4726.12 19119.33 ABCB7 ATP-binding cassette, sub-family B (MDR/TAP), member 7 1345.07 1420.86 1365.57 1268.81 1108.82 1149.20 4052.01 ABCB8 ATP-binding cassette, sub-family B (MDR/TAP), member 8 68.98 80.78 90.46 99.22 107.97 90.55 411.33 ABCC1 ATP-binding cassette, sub-family C (CFTR/MRP), member 1 1.69 23.33 2.19 3.43 9.52 9.88 735.97 ABCC2 ATP-binding cassette, sub-family C (CFTR/MRP), member 2 10233.04 8525.98 9247.76 8572.62 7020.57 7817.22 13791.13 ABCC3 ATP-binding cassette, sub-family C (CFTR/MRP), member 3 7518.63 10873.24 16616.45 12706.13 12103.09 20762.36 75939.51 ABCD1 ATP-binding cassette, sub-family D (ALD), member 1 159.49 201.47 202.15 244.30 236.99 220.86 350.43 ABCD3 ATP-binding cassette, sub-family D (ALD), member 3 736.43 612.80 586.77 627.10 391.93 477.00 1981.45 ABCD4 ATP-binding cassette, sub-family D (ALD), member 4 880.67 1110.88 1183.04 1055.09 661.97 663.88 3532.72 ABCG2 ATP-binding cassette, sub-family G (WHITE), member 2 2811.16 1994.83 2338.23 2336.77 2075.70 3022.38 1766.28 ABCG4 ATP-binding cassette, sub-family G (WHITE), member 4 2.39 3.89 7.90 2.74 2.48 2.20 74.11 ABCG5 ATP-binding cassette, sub-family G (WHITE), member 5 10030.65 4664.54 7619.57 7295.02 2156.45 2672.19 1995.26 ABCG8 ATP-binding cassette, sub-family G (WHITE), member 8 87.66 109.84 143.80 164.23 60.94 86.69 387.32 ADH1B alcohol dehydrogenase 1B (class I), beta polypeptide 3355.50 1052.20 1596.78 704.33 16.35 81.43 3375.10 AK3L2 adenylate kinase 3-like 2 1541.47 1650.12 1617.27 1344.14 888.75 780.19 2055.84 ANKRD18A ankyrin repeat domain 18A 20.85 101.05 42.10 41.12 12.30 55.17 336.15 ATP11C ATPase, class VI, type 11C 600.45 709.66 712.26 697.11 472.92 487.14 688.69 CCL2 chemokine (C-C motif) ligand 2 100.86 4692.64 212.61 883.38 43349.70 21663.60 3567.26 CCNB1 cyclin B1 60.14 113.21 76.92 124.58 174.63 154.57 1034.00 CCND1 cyclin D1 61.20 191.13 168.76 172.89 481.02 268.47 1938.49 CDC2 cell division cycle 2, G1 to S and G2 to M 2.97 10.91 3.50 21.29 12.39 12.32 2909.83 CDC2L2 Cell division cycle 2-like 2 (PITSLRE proteins) 73.78 109.55 96.77 74.09 67.49 82.76 729.20 CDC6 cell division cycle 6 homolog (S. cerevisiae) 40.09 49.42 59.96 52.26 73.03 109.25 1596.11 CROT carnitine O-octanoyltransferase 128.30 158.79 110.35 121.16 87.59 56.26 921.79 CRP C-reactive protein, pentraxin-related 11.13 185.06 57.94 51.08 676.93 765.18 701.77 CYP1A1 cytochrome P450, family 1, subfamily A, polypeptide 1 27.51 63.26 73.67 112.57 32.52 76.42 225.79 CYP1A2 cytochrome P450, family 1, subfamily A, polypeptide 2 805.83 856.33 1121.52 1577.54 250.18 366.28 811.23 CYP1B1 cytochrome P450, family 1, subfamily B, polypeptide 1 366.86 351.27 341.74 281.16 191.42 195.96 2624.95 CYP2A6 cytochrome P450, family 2, subfamily A, polypeptide 6 21852.20 9811.85 12044.00 1676.03 1041.71 385.29 4730.14 CYP2B6 cytochrome P450, family 2, subfamily B, polypeptide 6 32752.94 15866.03 15330.56 13332.79 15097.85 8823.01 9838.97 CYP2C18 cytochrome P450, family 2, subfamily C, polypeptide 18 19045.02 24596.46 22426.98 25409.16 19967.10 20887.69 3498.10 CYP2C19 cytochrome P450, family 2, subfamily C, polypeptide 19 21225.20 12942.72 17534.55 15723.74 2323.39 2676.69 19148.31 CYP2C8 cytochrome P450, family 2, subfamily C, polypeptide 8 13556.76 7043.40 10360.58 7287.89 451.97 918.45 8240.05 CYP2C9 cytochrome P450, family 2, subfamily C, polypeptide 9 66006.50 58821.84 68860.59 46485.20 11595.16 15408.15 58214.34 CYP2D6 cytochrome P450, family 2, subfamily D, polypeptide 6 3095.31 1481.71 2640.80 2335.44 342.73 680.93 102.76 CYP2E1 cytochrome P450, family 2, subfamily E, polypeptide 1 10847.38 12166.37 14574.39 15078.34 4599.93 1778.54 2859.10 CYP3A4 cytochrome P450, family 3, subfamily A, polypeptide 4 122248.70 65885.79 107782.20 46811.34 3872.06 7344.10 17894.76 DMD #42028

CYP3A5 cytochrome P450, family 3, subfamily A, polypeptide 5 15590.35 13206.06 15546.33 11660.02 6711.16 7565.16 10747.37 CYP3A7 cytochrome P450, family 3, subfamily A, polypeptide 7 86760.32 60642.05 72828.29 39119.79 17833.63 18903.58 18593.44 CYP4A11 cytochrome P450, family 4, subfamily A, polypeptide 11 599.94 328.82 541.92 299.23 68.33 81.92 1798.92 CYP4B1 cytochrome P450, family 4, subfamily B, polypeptide 1 2.02 2.30 2.42 2.36 1.83 2.14 30665.77 CYP7A1 cytochrome P450, family 7, subfamily A, polypeptide 1 7137.73 1472.14 1597.33 238.16 10.08 85.01 3389.15 CYP8B1 cytochrome P450, family 8, subfamily B, polypeptide 1 53472.84 41843.94 50875.07 38874.58 13968.59 22569.78 13697.15 G6PC glucose-6-phosphatase, catalytic subunit 792.81 129.31 321.05 117.36 20.79 103.23 2645.17 GLS2 glutaminase 2 (liver, mitochondrial) 1870.92 1059.78 1744.78 3338.50 507.93 567.53 1553.38 GSTA1 glutathione S-transferase A1 12835.29 7313.03 7046.94 4006.48 3043.50 4436.92 132174.14 GSTA2 glutathione S-transferase A2 19079.46 12146.50 14000.58 7885.15 7835.02 8940.39 156444.44 GSTA3 glutathione S-transferase A3 7428.07 4870.79 4125.35 3064.37 2025.36 2909.73 104941.18 GSTA4 glutathione S-transferase A4 742.57 1088.28 897.66 713.56 366.23 583.59 1271.29 GSTA5 glutathione S-transferase A5 20730.74 12869.37 16552.82 7323.20 7943.03 9544.20 172713.91 GSTK1 glutathione S-transferase kappa 1 2660.97 3421.33 3231.59 2477.01 1686.68 2225.51 7974.88 GSTM1 glutathione S-transferase M1 134.42 296.98 148.01 544.44 549.83 215.24 696.45 GSTM2 glutathione S-transferase M2 (muscle) 19.64 51.73 26.18 28.90 34.51 27.33 332.09 GSTM3 glutathione S-transferase M3 (brain) 1408.34 917.53 1757.79 4501.24 7147.25 2238.52 382.00 GSTM4 glutathione S-transferase M4 102.76 544.58 126.87 534.79 331.51 151.27 1702.87 GSTM5 glutathione S-transferase M5 40.82 162.16 35.05 134.76 51.85 40.45 115.70 GSTO1 glutathione S-transferase omega 1 32595.79 28088.99 32277.48 31921.33 20177.97 23478.58 84031.16 GSTP1 glutathione S-transferase pi 381.33 18694.40 1268.11 2464.42 9057.18 1835.39 32.07 GSTT1 glutathione S-transferase theta 1 1673.58 1902.74 2197.52 4247.26 1365.08 1548.33 4327.36 GSTT2 glutathione S-transferase theta 2 771.36 674.54 3624.29 138.64 450.74 357.17 3292.19 GYS1 glycogen synthase 1 (muscle) 59.09 116.51 112.39 132.10 122.98 152.38 3653.22 GYS2 glycogen synthase 2 (liver) 212.02 154.06 162.86 175.57 34.13 86.58 235.92 HK2 hexokinase 2 40.24 30.77 4.29 35.79 26.28 50.43 348.17 ID1 inhibitor of DNA binding 1 87.35 1865.83 352.13 1141.34 8233.91 3291.92 13947.58 IL6 interleukin 6 (interferon, beta 2) 7.65 20.05 8.67 8.13 29.70 18.02 634.69 IL8 interleukin 8 11.01 535.67 68.73 122.48 1904.09 1931.49 1172.45 IQSEC3 IQ motif and Sec7 domain 3 69.52 141.05 108.93 91.08 104.49 157.49 959.00 ITGA6 integrin, alpha 6 940.73 2242.44 1225.20 1363.68 1173.62 1243.42 2779.24 KRT19 keratin 19 212.46 1284.85 1524.38 2784.58 18759.64 1248.98 438659.56 LPL lipoprotein lipase 5.03 109.48 4.09 48.62 63.89 107.24 17122.36 MAG myelin associated glycoprotein 30.22 46.48 28.90 25.49 22.62 38.11 53.48 MAST4 microtubule associated serine/threonine kinase family member 4 111.86 347.79 214.37 104.23 214.01 180.21 160.88 MUC5B mucin 5B, oligomeric mucus/gel-forming 98.23 67.94 61.18 43.72 47.99 50.81 79.14 NR1I2 nuclear receptor subfamily 1, group I, member 2 1280.11 1583.55 1743.94 2123.87 756.28 1226.49 2849.87 NR1I3 nuclear receptor subfamily 1, group I, member 3 4372.04 3593.94 4396.47 5326.02 751.10 1014.35 1578.57 PANK2 pantothenate kinase 2 (Hallervorden-Spatz syndrome) 346.20 388.95 480.03 511.51 519.12 470.66 2081.87 PCDH15 protocadherin 15 1.81 2.20 2.38 51.50 1.75 2.04 6.72 PRG2 plasticity-related gene 2 5.04 8.33 7.42 15.02 7.59 15.87 32.90 SCD stearoyl-CoA desaturase (delta-9-desaturase) 6387.03 4304.54 3490.19 4979.74 1641.27 2086.32 12601.06 SLC10A1 solute carrier family 10, member 1 6349.59 6439.49 8601.18 9517.40 2333.55 4296.67 2388.22 STAT3 signal transducer and activator of transcription 3 125.26 445.58 282.65 438.76 586.12 511.64 1707.19 UGT1A6 UDP glucuronosyltransferase 1 family, polypeptide A6 26742.89 22432.98 25888.48 23396.19 14742.54 14773.91 76495.72 UGT1A8 UDP glucuronosyltransferase 1 family, polypeptide A8 1311.95 1085.12 1173.22 660.03 99.06 128.84 8923.29 UGT2B10 UDP glucuronosyltransferase 2 family, polypeptide B10 97307.70 51140.00 79815.04 38157.33 18158.80 33385.90 62976.09 UGT2B11 UDP glucuronosyltransferase 2 family, polypeptide B11 61511.58 32917.77 48652.55 25133.39 11650.58 24340.17 57241.30 UGT2B15 UDP glucuronosyltransferase 2 family, polypeptide B15 17895.15 15153.98 17903.04 12655.68 1401.86 2020.51 57875.84 UGT2B17 UDP glucuronosyltransferase 2 family, polypeptide B17 18873.20 12439.82 17572.27 10116.09 2370.88 5495.25 30706.93 UGT2B28 UDP glucuronosyltransferase 2 family, polypeptide B28 12175.01 6517.27 8902.42 2182.55 993.89 2563.55 4898.08 UGT2B4 UDP glucuronosyltransferase 2 family, polypeptide B4 48908.05 27819.35 49736.63 22529.01 8320.08 20269.05 40771.50 UGT2B7 UDP glucuronosyltransferase 2 family, polypeptide B7 97110.26 52618.81 74590.88 25838.88 12841.03 24127.98 23621.23