Model Name: "Jamshidi2007
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SBML Model Report Model name: “Jamshidi2007 - Genome-scale metabolic network of Mycobacterium tuberculosis (iNJ661)” 2LATEX July 28, 2015 1 General Overview This is a document in SBML Level 3 Version 1 format. Table1 shows an overview of the quantities of all components of this model. Table 1: Number of components in this model, which are described in the following sections. Element Quantity Element Quantity compartment types 0 compartments 2 species types 0 species 826 events 0 constraints 0 reactions 1025 function definitions 0 global parameters 19 unit definitions 1 rules 0 initial assignments 0 Model Notes Jamshidi2007 - Genome-scale metabolic networkof Mycobacterium tuberculosis (iNJ661) This model is described in the article:Investigating the metabolic capabilities of Mycobac- terium tuberculosis H37Rv using the in silico strain iNJ661 and proposing alternative drug tar- gets.Jamshidi N, Palsson B.BMC Syst Biol 2007; 1: 26 Abstract: Produced by SBML2LATEX 1 BACKGROUND: Mycobacterium tuberculosis continues to be a major pathogen in the third world, killing almost 2 million people a year by the most recent estimates. Even in industrialized countries, the emergence of multi-drug resistant (MDR) strains of tuberculosis hails the need to develop additional medications for treatment. Many of the drugs used for treatment of tuber- culosis target metabolic enzymes. Genome-scale models can be used for analysis, discovery, and as hypothesis generating tools, which will hopefully assist the rational drug development process. These models need to be able to assimilate data from large datasets and analyze them. RESULTS: We completed a bottom up reconstruction of the metabolic network of Mycobac- terium tuberculosis H37Rv. This functional in silico bacterium, iNJ661, contains 661 genes and 939 reactions and can produce many of the complex compounds characteristic to tuberculosis, such as mycolic acids and mycocerosates. We grew this bacterium in silico on various media, analyzed the model in the context of multiple high-throughput data sets, and finally we ana- lyzed the network in an ’unbiased’ manner by calculating the Hard Coupled Reaction (HCR) sets, groups of reactions that are forced to operate in unison due to mass conservation and con- nectivity constraints. CONCLUSION: Although we observed growth rates comparable to ex- perimental observations (doubling times ranging from about 12 to 24 hours) in different media, comparisons of gene essentiality with experimental data were less encouraging (generally about 55%). The reasons for the often conflicting results were multi-fold, including gene expression variability under different conditions and lack of complete biological knowledge. Some of the inconsistencies between in vitro and in silico or in vivo and in silico results highlight specific loci that are worth further experimental investigations. Finally, by considering the HCR sets in the context of known drug targets for tuberculosis treatment we proposed new alternative, but equivalent drug targets. This model is hosted on BioModels Database and identified by: MODEL1507180001. To cite BioModels Database, please use: BioModels Database: An enhanced, curated and annotated resource for published quantitative kinetic models. To the extent possible under law, all copyright and related or neighbouring rights to this en- coded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information. 2 Unit Definitions This is an overview of six unit definitions of which five are predefined by SBML and not men- tioned in the model. 2.1 Unit mmol per gDW per hr −1 Definition mmol · g−1 · (3600 s) 2.2 Unit substance Notes Mole is the predefined SBML unit for substance. Definition mol 2 Produced by SBML2LATEX 2.3 Unit volume Notes Litre is the predefined SBML unit for volume. Definition l 2.4 Unit area Notes Square metre is the predefined SBML unit for area since SBML Level 2 Version 1. Definition m2 2.5 Unit length Notes Metre is the predefined SBML unit for length since SBML Level 2 Version 1. Definition m 2.6 Unit time Notes Second is the predefined SBML unit for time. Definition s 3 Compartments This model contains two compartments. Table 2: Properties of all compartments. Id Name SBO Spatial Size Unit Constant Outside Dimensions c Cytosol 3 1 litre 3 e Extraorganism 3 1 litre 3 2 2 3.1 Compartment c This is a three dimensional compartment with a constant size given in litre. Name Cytosol 3.2 Compartment e This is a three dimensional compartment with a constant size given in litre. Name Extraorganism Produced by SBML2LATEX 3 4 4 Species This model contains 826 species. Section7 provides further details and the derived rates of change of each species. Table 3: Properties of each species. Id Name Compartment Derived Unit Constant Boundary Condi- tion M 10fthf c 10-Formyltetrahydrofolate c mol · l−1 – – M 12dgr160 c 1,2-Diacyl-sn-glycerol (dihexadecanoyl, c mol · l−1 – – Produced by n-C16:0) 2 2 − 2 2 M 13dpg c 3-Phospho-D-glyceroyl phosphate c mol · l 1 – – M 1hdecg3p c 1-hexadecanoyl-sn-glycerol 3-phosphate c mol · l−1 – – − 2 2 M 1msg3p c 1-Acyl-sn-glycerol 3-phosphate (branched c mol · l 1 – – 2 2 SBML C19:0) − 2 2 M 1pyr5c c 1-Pyrroline-5-carboxylate c mol · l 1 – – 2 −1 L A M 23dhba c (2,3-Dihydroxybenzoyl)adenylate c mol · l – – T E −1 2 2 X M 23dhdp c 2,3-Dihydrodipicolinate c mol · l – – − 2 2 M 23dhmb c (R)-2,3-Dihydroxy-3-methylbutanoate c mol · l 1 – – − 2 2 M 23dhmp c (R)-2,3-Dihydroxy-3-methylpentanoate c mol · l 1 – – − 2 2 M 25aics c (S)-2-[5-Amino-1-(5-phospho- c mol · l 1 – – D-ribosyl)imidazole-4- 2 2 carboxamido]succinate 2 2 M 25dhpp c 2,5-Diamino-6-hydroxy-4-(5’- c mol · l−1 – – phosphoribosylamino)-pyrimidine − 2 2 M 25drapp c 2,5-Diamino-6-(ribosylamino)-4-(3H)- c mol · l 1 – – pyrimidinone 5’-phosphate − 2 2 M 26dap DASH LL c LL-2,6-Diaminoheptanedioate c mol · l 1 – – M 26dap DASH M c meso-2,6-Diaminoheptanedioate c mol · l−1 – – 2 2 2 2 Id Name Compartment Derived Unit Constant Boundary Condi- tion M 2agpe160 c 2-Acyl-sn-glycero-3- c mol · l−1 – – phosphoethanolamine (n-C16:0) − 2 2 M 2agpe180 c 2-Acyl-sn-glycero-3- c mol · l 1 – – phosphoethanolamine (n-C18:0) − 2 2 M 2ahbut c (S)-2-Aceto-2-hydroxybutanoate c mol · l 1 – – M 2ahhmd c 2-Amino-4-hydroxy-6-hydroxymethyl- c mol · l−1 – – 7,8-dihydropteridine diphosphate 2 2 −1 2 2 Produced by M 2ahhmp c 2-Amino-4-hydroxy-6-hydroxymethyl- c mol · l – – 7,8-dihydropteridine − 2 2 M 2c25dho c 2-Carboxy-2,5-dihydro-5-oxofuran-2- c mol · l 1 – – acetate − 2 2 M 2cpr5p c 1-(2-Carboxyphenylamino)-1-deoxy-D- c mol · l 1 – – SBML ribulose 5-phosphate −1 2 2 2 M 2dda7p c 2-Dehydro-3-deoxy-D-arabino-heptonate c mol · l – – L A T E 7-phosphate X − 2 2 M 2dglcn c 2-Deoxy-D-gluconate c mol · l 1 – – M 2dhp c 2-Dehydropantoate c mol · l−1 – – − 2 2 M 2dmmq6 c 2-Demethylmenaquinone 6 c mol · l 1 – – − 2 2 M 2dmmq8 c 2-Demethylmenaquinone 8 c mol · l 1 – – − 2 2 M 2dmmql8 c 2-Demethylmenaquinol 8 c mol · l 1 – – − 2 2 M 2dr1p c 2-Deoxy-D-ribose 1-phosphate c mol · l 1 – – − 2 2 M 2dr5p c 2-Deoxy-D-ribose 5-phosphate c mol · l 1 – – − 2 2 M 2ippm c 2-Isopropylmaleate c mol · l 1 – – − 2 2 M 2mahmp c 2-Methyl-4-amino-5- c mol · l 1 – – hydroxymethylpyrimidine diphosphate 2 2 − 2 2 M 2me4p c 2-C-methyl-D-erythritol 4-phosphate c mol · l 1 – – 5 2 2 6 Id Name Compartment Derived Unit Constant Boundary Condi- tion M 2mecdp c 2-C-methyl-D-erythritol 2,4- c mol · l−1 – – cyclodiphosphate − 2 2 M 2mop c 2-Methyl-3-oxopropanoate c mol · l 1 – – M 2obut c 2-Oxobutanoate c mol · l−1 – – − 2 2 M 2p4c2me c 2-phospho-4-(cytidine 5’-diphospho)-2- c mol · l 1 – – C-methyl-D-erythritol 2 2 − 2 2 M 2pg c D-Glycerate 2-phosphate c mol · l 1 – – −1 Produced by M 2pglyc c 2-Phosphoglycolate c mol · l – – − 2 2 M 2shchc c 2-Succinyl-6-hydroxy-2,4- c mol · l 1 – – cyclohexadiene-1-carboxylate 2 2 − 2 2 M 34hpp c 3-(4-Hydroxyphenyl)pyruvate c mol · l 1 – – M 35cgmp c 3’,5’-Cyclic GMP c mol · l−1 – – SBML − 2 2 M 3c2hmp c 3-Carboxy-2-hydroxy-4- c mol · l 1 – – 2 2 2 methylpentanoate L A − T · 1 2 2 E M 3c3hmp c 3-Carboxy-3-hydroxy-4- c mol l – – X methylpentanoate − 2 2 M 3c4mop c 3-Carboxy-4-methyl-2-oxopentanoate c mol · l 1 – – M 3ddgc c 3-Dehydro-2-deoxy-D-gluconate c mol · l−1 – – − 2 2 M 3dhq c 3-Dehydroquinate c mol · l 1 – – − 2 2 M 3dhsk c 3-Dehydroshikimate c mol · l 1 – – − 2 2 M 3ig3p c C’-(3-Indolyl)-glycerol 3-phosphate c mol · l 1 – – − 2 2 M 3mob c 3-Methyl-2-oxobutanoate c mol · l 1 – – − 2 2 M 3mop c (S)-3-Methyl-2-oxopentanoate c mol · l 1 – – − 2 2 M 3pg c 3-Phospho-D-glycerate c mol · l 1 – – − 2 2 M 3php c 3-Phosphohydroxypyruvate c mol · l 1 – – − 2 2 M 3psme c 5-O-(1-Carboxyvinyl)-3- c mol · l 1 – – 2 2 phosphoshikimate 2 2 Id Name Compartment Derived Unit Constant Boundary Condi- tion M 4abut c 4-Aminobutanoate c mol · l−1 – – M 4abz c 4-Aminobenzoate c mol · l−1 – – − 2 2 M 4adcho c 4-amino-4-deoxychorismate c mol · l 1 – – − 2 2 M 4ahmmp c 4-Amino-5-hydroxymethyl-2- c mol · l 1 – – methylpyrimidine 2 2 − 2 2 M 4ampm c 4-Amino-2-methyl-5- c mol · l 1 – – phosphomethylpyrimidine −1 2 2 Produced by M 4c2me c 4-(cytidine 5’-diphospho)-2-C-methyl-D- c mol · l – – erythritol − 2 2 M 4h2opntn c 4-Hydroxy-2-oxopentanoate c mol · l 1 – – M 4hba c 4-Hydroxy-benzyl alcohol c mol · l−1 – – − 2 2 M 4hbzACP c 4-hydroxybenzoyl ACP c mol · l 1 – – SBML − 2 2 M 4hbz c 4-Hydroxybenzoate c mol · l 1 – – −1 2 2 2 M 4hthr c 4-Hydroxy-L-threonine c mol · l – – L A − T · 1 2 2 E M 4mhetz c 4-Methyl-5-(2-hydroxyethyl)-thiazole c mol l – – X − 2 2 M 4mop c 4-Methyl-2-oxopentanoate