DOCSLIB.ORG

The Ultrastructure and Function of the Gut of Patella Vulgata

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
The Ultrastructure and Function of the Gut of Patella Vulgata THE ULTRASTRUCTURE AND FUNCTION OF THE GUT OF PATELLA VULGATA. by Maxwell Simon Bush, Department of Zoology, Bedford College, University of London. Thesis submitted for the degree of PhD. ProQuest Number: 10098528 All rights reserved INFORMATION TO ALL USERS The quality of this reproduction is dependent upon the quality of the copy submitted. In the unlikely event that the author did not send a complete manuscript and there are missing pages, these will be noted. Also, if material had to be removed, a note will indicate the deletion. uest. ProQuest 10098528 Published by ProQuest LLC(2016). Copyright of the Dissertation is held by the Author. All rights reserved. This work is protected against unauthorized copying under Title 17, United States Code. Microform Edition © ProQuest LLC. ProQuest LLC 789 East Eisenhower Parkway P.O. Box 1346 Ann Arbor, Ml 48106-1346 R.H Q N.( ifij-c'îAnYj CLASS S%:M No. 6 ^ 1 ACC No Dats ACO. Klov’ gt I dedicate this work to my parents, whose co-operation and financial support made it possible. ABSTRACT. The limpet gut consists of a long coiled tube lined by a ciliated columnar epithelium, into which open the ducts of the salivary and digestive glands. Six sections can be distinguished: the buccal cavity, oesophagus, stomach, style sac, intestine and rectum. The oesophagus can be subdivided into a dorsal food channel and a series of lateral pouches forming the oesophageal gland. The intestine is divisible into sections designated A, B, C, D and E. Ultrastructural and histochemical analyses revealed nine cell types, seven of them glandular. A single type of gland cell lining the tubules of the salivary gland produces a viscous secretion that lubricates the radula and entraps particles rasped from the substratum. Mucous cells occur in the buccal cavity, dorsal food channel and rectum; in the rectum, mucus aids defaecation, but elsewhere it entraps loose particles that are consequently transported to the stomach. The only extracellular enzyme, an amylase derived from the gland cells of the oesophageal gland, is mixed with the food in the dorsal food channel. Ciliated and unciliated columnar cells lining the ducts of the digestive gland, stomach, style sac and anterior intestine, release blebs of cytoplasm into the lumen to consolidate loose particles into a faecal rod that is rotated along the intestine. Clavate gland cells and possibly basal gland cells in the posterior intestine, cover the faecal rod with their secretion to form a durable rod. The vacuolated digestive cells of the digestive gland, digest food intracellularly releasing undigestible residues in spherules of cytoplasm, these are bound into a liver string by the proteinaceous secretion of the basophilic cells. Both these cell types and the amylase-secreting cells exhibit phases of activity, but only that of the latter is related to the tidal cycle. Tritiated D-glucose was absorbed by the oesophagus, intestine and digestive gland by a mechanism inhibited by 2,4-DNP and phloridzin. The mechanisms operating in the oesophagus and posterior intestine were sodium-dependent. Fluid movements from the intestinal lumen to the blood occurred. TABLE OF CONTENTS, PAGE. TITLE 1. DEDICATION 2 . ABSTRACT 3-4. TABLE OF CONTENTS 5-9. LIST OF PLATES . 10-11. LIST OF FIGURES AND TABLES 12-14. INTRODUCTION 15-31. MATERIALS AND METHODS 32-52. Collection site 32. Collection, transport and maintenance 32-33. Dissection techniques 33. Preparation of sections 33-35. 1.Wax .... 33-34. 2 .Thick resin . 34. 3.Frozen . 34-35. Preparation of tissue for TEM 35-36. Preparation of tissue for SEM 36-37. Histochemistry 37-38. Biochemical enzyme demonstrations 38-39. Cytochemistry 39-42. 1.Acid phosphatase 39. 2 .Alkaline phophatase 39. 3 .Carbohydrates . 39-41. A).PTA 39-40. B).PA-SM and PA-TSC-SP 40-41. 4.Tannic acid 41. 5.Ferritin tracing 41-42. pH of gut fluid 42. Ciliary currents 42. Phasic activity 43. Starvation 43. Radiotracer experiments 43-47. l.In vitro experiments 43-47. A).Glucose absorption 43-45. B).Glucose flux 45-46. C).Chromatography 46-47. D).Net water movement 47. 2 .In vivo experiments 47. 3.Statistical methods 48. Autoradiography 48-49. Photographic techniques 49. Word processing 49. RESULTS 53- THE CILIATED AND UNCILIATED EPITHELIUM 53-82. Ultrastructure . 56-66. Cyto- and histochemistry 66-72. 1 .General 66-67. 2 .Carbohydrates . 68-70. 3.Proteins 70. 4.Lipids 70. 5.Pigments 70. 6 .Enzymes 70-72. Plates 1-7 73-82. THE BUCCAL CAVITY 83-89. Plates 8-9 86-89. THE SALIVARY GLANDS . 90-103. l.The ducts 90-93. 2 .The tubules .... 93-98. A).Histology and ultrastructure 93-96. B).Histochemistry 96-98. Plates 10-12 99-103. THE OESOPHAGUS 104-133. 1 .Anatomy 104-108. 2 .Ciliary currents 108-109. 3.Epithelium of food channel 109-113. A).The ciliated cells 109. B).The mucous gland cells 109-113. 4.Epithelium of oesophageal gland 113-120. A).The ciliated cells 115. B).The amylase-secreting cells 115-120. Plates 13-22 121-133. THE STOMACH AND STYLE SAC . 134-151. l.SEM 134-140. 2 .Ultrastructure and histochemistry 140-141. 3.Ciliary currents 141-144. Plates 23-27 145-151. THE DIGESTIVE GLAND . 152-184. 1.The ducts 152-155. A).Ultrastructure 152-153. B).Histochemistry 153-155. 2 .The tubules 155-173. A) .Basophilic cell 155-163. i).Ultrastructure . 155-161. ii).Histochemistry . 161-163. B).Digestive cell 163-173. i).Ultrastructure . 163-168. ii).Histochemistry . 168-173. 8 Plates 28-35 174-184. THE INTESTINE . 185-210. 1.Section A ' 185. 2.Section B 185-187. 3.Section C 187-193. A).Basai gland cell 187-193. i).Ultrastructure 187-191. ii).Histochemistry 191-193 4.Section D 193-197. A).Clavate gland cell 193-197. i).Ultrastructure 193-195. ii).Histochemistry 195-197. 5.Section E 197. Plates 36-45 198-210. THE RECTUM 211-216. Plates 46-47 214-216. THE EFFECTS OF STARVATION ON THE GUT 217-219. Plate 48 . 220-221. PHASIC ACTIVITY 222-253. 1.The oesophageal gland 222-234. A).Ultrastructural characteristics 222-229. B).Synchrony of phases in gland 229-233. C).Synchrony of phases with tide 233-234. 2 .The digestive gland . 234-241. A) .Digestive cell 234-238. i).Holding phase 234-236. ii).Absorbing phase 236. iii).Digesting phase 236-237. iv).Fragmenting phase 237-238. v).Disintegrating phase . 238. B).Basophilic cell 238. C).Synchrony of phases with tide 238-241. Plates 49-57 242-253. RADIOTRACER EXPERIMENTS 254-288. l.In vivo experiments . 254-258. 2 .Glucose flux experiments 258-267. 3.Glucose absorption experiments 268-280. A).Absorption 258&268. B).Inhibitors 268-272. C).Sodium concentration 272. D).Effect of pH . 272-274. E).Kinetics 274-280. 4.Chromatographic experiments 280-285. 5.Net water movement experiments 285-288. AUTORADIOGRAPHY 289. DISCUSSION 290-339. SUMMARY 340-341. ACKNOWLEDGEMENTS 342. REFERENCES 343-367. APPENDICES 368-374. lA . 368-370. IB . 370-371. 2 371-373. • 373-374. 10 LIST OF p l a t e s . PLATES. PAGE. Abbreviations used in plates 1-7 • 73. 1-7 Ciliated and unciliated columnar cells. 74-82. 1-5 Transmission Electron Micrographs (TEM) of cells • 76-80. 6-7 Cytochemistry of cells • 81-82. Abbreviations used in plates 8-9 • 86. 8-9 Scanning Electron Micrographs (SEM) of buccal cavity 87-89. Abbreviations used in plates 10-12 . • 99. 10-12 Salivary glands. 100-103. 10 SEM of glands, TEM of the duct cells • 101. 11-12 TEM of the tubule cells • 102-103. Abbreviations used in plates 13-22 . • 121. 13-22 The oesophagus. 122-133. 13 Light micrograph (LM) and SE>1 of oesophagus • 124. 14 TEM of oesophageal epithelia • 125. 15 TEM of epithelium of food channel . • 126 16 LM post-oesophagus, SEM food channel epithelium • 127. 17 SEM and TEM of mucous cells . • 128. 18 SEM of oesophageal gland • 129. 19-21 TEM of amylase-secreting cell • 130-132. 22 Amoebocytic blood cell, starch-film method • 133. Abbreviations used in plates 23-27 . • 145. 23-27 The stomach and style sac. 146-151. 23 SEM of stomach .... •147. 24 SEM of digestive gland duct and style sac . • 148. 25 SEM of typhlosoles .... •149. 26 LM of typhlosoles and SEM of white secretion • 150. 27 TEM secretory tract cells, SEM liver string • 151. Abbreviations used in plates 28-35 . 174. 11 28-35 The digestive gland. 175-184. 28 TEM of duct cells, LM of tubule 177. 29 TEM of tubule epithelium . 178. 30-31 TEM of basophilic cell 179-180. 32 TEM digestive cells (absorbing & digesting) 181. 33-34 TEM digestive cell .... 182-183. 35 Starch-film and gelatin-film methods 184. Abbreviations used in plates 36-45 . 198. 36-45 The intestine. 199-210. 36 SEM and TEM of section A . 201. 37 SEM and TEM of section B 202. 38 SEM and TEM of section C 203. 39 TEM and SEM of basal gland cell (bgc) 204. 40-41 TEM and cytochemistry of bgc, TEM of section D 205-206. 42-44 TEM and SEM of clavate gland cell . 207-209. 45 TEM and SEM of section E 210. Abbreviations used in plates 46-47. 214. 46-47 SEM, LM and TEM of rectum 215-216. Abbreviations used in plate 48 220. 48 TEM of starved digestive gland cells 221. Abbreviations used in plates 49-57 . 242. 49-57 Phasic activity. 243-253. 49 LM of oesophageal gland during both phases 245. 50-51 TEM of amylase-secreting cell (secretory phase) 246-247. 52 TEM of amylase-secreting cell (heterophagic phase 248.
Load more

Recommended publications
  • Food Handling and Mastication in the Carp (Cyprinus Carpio L.)
    KJAJA2Ö*)O)Ó Food handling and mastication in the carp (Cyprinus carpio L.) ««UB***1* WIT1 TUOSW«- «* omslag tekening : Wim Valen -2- Promotor: dr. J.W.M. Osse, hoogleraar in de algemene dierkunde ^iJOttO^ 1oID Ferdinand A. Sibbing FOOD HANDLING AND MASTICATION IN THE CARP (Cyprinus carpio L.) Proefschrift ter verkrijging van de graad van doctor in de landbouwwetenschappen, op gezag van de rector magnificus, dr. C.C.Oosterlee, in het openbaar te verdedigen op dinsdag 11 december 1984 des namiddags te vier uur in de aula van de Landbouwhogeschool te Wageningen. l^V-, ^v^biS.oa BIBLIOTHEEK ^LANDBOUWHOGESCHOOL WAGENINGEN ^/y/of^OÏ, fOtO STELLINGEN 1. De taakverdeling tussen de kauwspieren van de karper is analoog aan die tussen vliegspieren van insekten: grote lichaamsspieren leveren indirekt het vermogen, terwijl direkt aangehechte kleinere spieren de beweging vooral sturen. Deze analogie komt voort uit architekturale en kinematische principes. 2. Naamgeving van spieren op grond van hun verwachte rol (b.v. levator, retractor) zonder dat deze feitelijk is onderzocht leidt tot lang doorwerkende misvattingen over hun funktie en geeft blijk van een onderschatting van de plasticiteit waarmee spieren worden ingezet. Een nomenclatuur die gebaseerd is op origo en insertie van de spier verdient de voorkeur. 3. De uitstulpbaarheid van de gesloten bek bij veel cypriniden maakt een getrapte zuivering van het voedsel mogelijk en speelt zo een wezenlijke rol in de selektie van bodemvoedsel. Dit proefschrift. 4. Op grond van de vele funkties die aan slijm in biologische systemen worden toegeschreven is meer onderzoek naar zijn chemische en fysische eigenschappen dringend gewenst. Dit proefschrift.
    [Show full text]
  • Copyrighted Material
    319 Index a oral cavity 195 guanocytes 228, 231, 233 accessory sex glands 125, 316 parasites 210–11 heart 235 acidophils 209, 254 pharynx 195, 197 hemocytes 236 acinar glands 304 podocytes 203–4 hemolymph 234–5, 236 acontia 68 pseudohearts 206, 208 immune system 236 air sacs 305 reproductive system 186, 214–17 life expectancy 222 alimentary canal see digestive setae 191–2 Malpighian tubules 232, 233 system taxonomy 185 musculoskeletal system amoebocytes testis 214 226–9 Cnidaria 70, 77 typhlosole 203 nephrocytes 233 Porifera 28 antennae nervous system 237–8 ampullae 10 Decapoda 278 ocelli 240 Annelida 185–218 Insecta 301, 315 oral cavity 230 blood vessels 206–8 Myriapoda 264, 275 ovary 238 body wall 189–94 aphodus 38 pedipalps 222–3 calciferous glands 197–200 apodemes 285 pharynx 230 ciliated funnel 204–5 apophallation 87–8 reproductive system 238–40 circulatory system 205–8 apopylar cell 26 respiratory system 236–7 clitellum 192–4 apopyle 38 silk glands 226, 242–3 coelomocytes 208–10 aquiferous system 21–2, 33–8 stercoral sac 231 crop 200–1 Arachnida 221–43 sucking stomach 230 cuticle 189 biomedical applications 222 taxonomy 221 diet 186–7 body wall 226–9 testis 239–40 digestive system 194–203 book lungs 236–7 tracheal tube system 237 dissection 187–9 brain 237 traded species 222 epidermis 189–91 chelicera 222, 229 venom gland 241–2 esophagus 197–200 circulatory system 234–6 walking legs 223 excretory system 203–5 COPYRIGHTEDconnective tissue 228–9 MATERIALzoonosis 222 ganglia 211–13 coxal glands 232, 233–4 archaeocytes 28–9 giant nerve
    [Show full text]
  • Are the Traditional Medical Uses of Muricidae Molluscs Substantiated by Their Pharmacological Properties and Bioactive Compounds?
    Mar. Drugs 2015, 13, 5237-5275; doi:10.3390/md13085237 OPEN ACCESS marine drugs ISSN 1660-3397 www.mdpi.com/journal/marinedrugs Review Are the Traditional Medical Uses of Muricidae Molluscs Substantiated by Their Pharmacological Properties and Bioactive Compounds? Kirsten Benkendorff 1,*, David Rudd 2, Bijayalakshmi Devi Nongmaithem 1, Lei Liu 3, Fiona Young 4,5, Vicki Edwards 4,5, Cathy Avila 6 and Catherine A. Abbott 2,5 1 Marine Ecology Research Centre, School of Environment, Science and Engineering, Southern Cross University, G.P.O. Box 157, Lismore, NSW 2480, Australia; E-Mail: [email protected] 2 School of Biological Sciences, Flinders University, G.P.O. Box 2100, Adelaide 5001, Australia; E-Mails: [email protected] (D.R.); [email protected] (C.A.A.) 3 Southern Cross Plant Science, Southern Cross University, G.P.O. Box 157, Lismore, NSW 2480, Australia; E-Mail: [email protected] 4 Medical Biotechnology, Flinders University, G.P.O. Box 2100, Adelaide 5001, Australia; E-Mails: [email protected] (F.Y.); [email protected] (V.E.) 5 Flinders Centre for Innovation in Cancer, Flinders University, G.P.O. Box 2100, Adelaide 5001, Australia 6 School of Health Science, Southern Cross University, G.P.O. Box 157, Lismore, NSW 2480, Australia; E-Mail: [email protected] * Author to whom correspondence should be addressed; E-Mail: [email protected]; Tel.: +61-2-8201-3577. Academic Editor: Peer B. Jacobson Received: 2 July 2015 / Accepted: 7 August 2015 / Published: 18 August 2015 Abstract: Marine molluscs from the family Muricidae hold great potential for development as a source of therapeutically useful compounds.
    [Show full text]
  • Contributions to Zoology, 71 (1/3) 37-45 (2002)
    Contributions to Zoology, 71 (1/3) 37-45 (2002) SPB Academic Publishing bv, The Hague Newly-discovered muscle in the larva of Patella coerulea (Mollusca, the of larval Gastropoda) suggests presence a extensor Peter Damen and Wim+J.A.G. Dictus Department ofExperimental Zoology, Utrecht University, Padualaan 8, 3584 CH Utrecht, Netherlands, [email protected] Key words: Patella, Gastropoda, Mollusca, development, larva, muscle, antagonist, cell-lineage Abstract and Dictus and Damen (1997). The formation of the 3D-macromere, which acts as an organizer and The developmentofthe muscular system ofthe gastropod mollusc is responsible for the correct dorsoventral pattern- Patella has been thoroughly studied. As a result, two larval of the has been described ing embryo, by e.g. van the main and larval had been retractors, accessory retractor, den Biggelaar and Guerrier (1979), Arnolds et al. described in ofPatella. the larva These muscles were supposed (1983), Verdonk and van den Biggelaar (1983), to be responsible for the retraction of the larval body into the Martindale et al. and Damen and Dictus shell.Previously no larval extensors, which would be responsible (1985), The of function for the extension of the larval body out ofthe shell, have been (1996). analysis gene during early described. Using cell-lineage tracer injection and phalloidin development has been described by van der Kooij staining ofmuscles, anewly-discovered muscle is herein identi- et al. (1996, 1998), Damen et al. (1994), Damen fied in the larva of Patella coerulea. A functional model is and Loon Klerkx van (1996), et al. (2001), Lartillot presented in which two muscles areresponsible for the extension et al.
    [Show full text]
  • Characterization of Calcineurin a and B Genes in the Abalone, Haliotis Diversicolor, and Their Immune Response Role During Bacterial Infection
    Characterization of calcineurin A and B genes in the abalone, Haliotis diversicolor, and their immune response role during bacterial infection Tiranan Buddawong1, Somluk Asuvapongpatana1, Saengchan Senapin2,3, Carmel McDougall4 and Wattana Weerachatyanukul1 1 Department of Anatomy, Faculty of Science, Mahidol University, Ratchathewi, Bangkok, Thailand 2 Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Faculty of Science, Mahidol University, Ratchathewi, Bangkok, Thailand 3 National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Klongluang, Pathumthani, Thailand 4 Australian Rivers Institute, Griffith University, Nathan, Queensland, Australia ABSTRACT Calcineurin (CN) is known to be involved in many biological processes, particularly, the immune response mechanism in many invertebrates. In this study, we characterized both HcCNA and HcCNB genes in Haliotis diversicolor, documented their expression in many tissues, and discerned their function as immune responsive genes against Vibrio parahaemolyticus infection. Similar to other mollusk CNs, the HcCNA gene lacked a proline-rich domain and comprised only one isoform of its catalytic unit, in contrast to CNs found in mammals. HcCNB was highly conserved in both sequence and domain architecture. Quantitative PCR and in situ hybridization revealed that the genes were broadly expressed and were not restricted to tissues traditionally associated with immune function. Upon infection of H. diversicolor with V. parahaemolyticus (a bacteria that causes serious disease in crustaceans and mollusks), both HcCNA and HcCNB genes were highly up-regulated at the early phase of bacterial infection. HcCNB was expressed significantly higher than HcCNA in response to bacterial challenge, Submitted 12 November 2019 Accepted 9 March 2020 suggesting its independent or more rapid response to bacterial infection.
    [Show full text]
  • Mollusca, Archaeogastropoda) from the Northeastern Pacific
    Zoologica Scripta, Vol. 25, No. 1, pp. 35-49, 1996 Pergamon Elsevier Science Ltd © 1996 The Norwegian Academy of Science and Letters Printed in Great Britain. All rights reserved 0300-3256(95)00015-1 0300-3256/96 $ 15.00 + 0.00 Anatomy and systematics of bathyphytophilid limpets (Mollusca, Archaeogastropoda) from the northeastern Pacific GERHARD HASZPRUNAR and JAMES H. McLEAN Accepted 28 September 1995 Haszprunar, G. & McLean, J. H. 1995. Anatomy and systematics of bathyphytophilid limpets (Mollusca, Archaeogastropoda) from the northeastern Pacific.—Zool. Scr. 25: 35^9. Bathyphytophilus diegensis sp. n. is described on basis of shell and radula characters. The radula of another species of Bathyphytophilus is illustrated, but the species is not described since the shell is unknown. Both species feed on detached blades of the surfgrass Phyllospadix carried by turbidity currents into continental slope depths in the San Diego Trough. The anatomy of B. diegensis was investigated by means of semithin serial sectioning and graphic reconstruction. The shell is limpet­ like; the protoconch resembles that of pseudococculinids and other lepetelloids. The radula is a distinctive, highly modified rhipidoglossate type with close similarities to the lepetellid radula. The anatomy falls well into the lepetelloid bauplan and is in general similar to that of Pseudococculini- dae and Pyropeltidae. Apomorphic features are the presence of gill-leaflets at both sides of the pallial roof (shared with certain pseudococculinids), the lack of jaws, and in particular many enigmatic pouches (bacterial chambers?) which open into the posterior oesophagus. Autapomor- phic characters of shell, radula and anatomy confirm the placement of Bathyphytophilus (with Aenigmabonus) in a distinct family, Bathyphytophilidae Moskalev, 1978.
    [Show full text]
  • Mollusca Gastropoda : Columbariform Gastropods of New Caledonia
    ÎULTATS DES CAMPAGNES MUSORSTOM. VOLUME 7 RÉSULTATS DES CAMPAGNES MUSORSTOM. VOLUME i RÉSUI 10 Mollusca Gastropoda : Columbariform Gastropods of New Caledonia M. G. HARASEWYCH Smithsonian Institution National Museum of Natural History Department of Invertebrate Zoology Washington, DC 20560 U.S.A. ABSTRACT A survey of the deep-water malacofauna of New Caledo­ Fustifusus. Serratifusus virginiae sp. nov. and Serratifusus nia has brought to light two species referable to the subfamily lineatus sp. nov., two Recent species of the columbariform Columbariinac (Gastropoda: Turbincllidae). Coluzca faeeta genus Serratifusus Darragh. 1969. previously known only sp. nov. is described from off the Isle of Pines at depths of from deep-water fossil deposits of Miocene age. arc also 385-500 m. Additional specimens of Coluzea pinicola Dar- described. On the basis of anatomical and radular data, ragh, 19X7, previously described from off the Isle of Pines, Serratifusus is transferred from the Columbariinae to the serve as the basis for the description of the new genus family Buccinidae. RESUME Mollusca Gastropoda : Gastéropodes columbariformes de également décrite de l'île des Pins, a été récoltée vivante et Nouvelle-Calédonie. devient l'espèce type du nouveau genre Fustifusus. Le genre Serratifusus Darragh. 1969 n'était jusqu'ici connu que de Au cours des campagnes d'exploration de la faune pro­ dépôts miocènes en faciès profond : deux espèces actuelles. .S. fonde de Nouvelle-Calédonie, deux espèces de la sous-famille virginiae sp. nov. et S. lineatus sp. nov., sont maintenant Columbariinae (Gastropoda : Turbinellidae) ont été décou­ décrites de Nouvelle-Calédonie. Sur la base des caractères vertes. Coluzea faeeta sp.
    [Show full text]
  • Deep-Water Buccinidae (Gastropoda: Neogastropoda) from Sunken Wood, Vents and Seeps: Molecular Phylogeny and Taxonomy Yu.I
    Deep-water Buccinidae (Gastropoda: Neogastropoda) from sunken wood, vents and seeps: molecular phylogeny and taxonomy Yu.I. Kantor, N. Puillandre, K. Fraussen, A.E. Fedosov, P. Bouchet To cite this version: Yu.I. Kantor, N. Puillandre, K. Fraussen, A.E. Fedosov, P. Bouchet. Deep-water Buccinidae (Gas- tropoda: Neogastropoda) from sunken wood, vents and seeps: molecular phylogeny and taxonomy. Journal of the Marine Biological Association of the UK, Cambridge University Press (CUP), 2013, 93 (8), pp.2177-2195. 10.1017/S0025315413000672. hal-02458197 HAL Id: hal-02458197 https://hal.archives-ouvertes.fr/hal-02458197 Submitted on 28 Jan 2020 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Deep-water Buccinidae (Gastropoda: Neogastropoda) from sunken wood, vents and seeps: Molecular phylogeny and taxonomy KANTOR YU.I.1, PUILLANDRE N.2, FRAUSSEN K.3, FEDOSOV A.E.1, BOUCHET P.2 1 A.N. Severtzov Institute of Ecology and Evolution of Russian Academy of Sciences, Leninski Prosp. 33, Moscow 119071, Russia, 2 Muséum National d’Histoire Naturelle, Departement Systematique et Evolution, UMR 7138, 43, Rue Cuvier, 75231 Paris, France, 3 Leuvensestraat 25, B–3200 Aarschot, Belgium ABSTRACT Buccinidae - like other canivorous and predatory molluscs - are generally considered to be occasional visitors or rare colonizers in deep-sea biogenic habitats.
    [Show full text]
  • Population Characteristics of the Limpet Patella Caerulea (Linnaeus, 1758) in Eastern Mediterranean (Central Greece)
    water Article Population Characteristics of the Limpet Patella caerulea (Linnaeus, 1758) in Eastern Mediterranean (Central Greece) Dimitris Vafidis, Irini Drosou, Kostantina Dimitriou and Dimitris Klaoudatos * Department of Ichthyology and Aquatic Environment, School of Agriculture Sciences, University of Thessaly, 38446 Volos, Greece; dvafi[email protected] (D.V.); [email protected] (I.D.); [email protected] (K.D.) * Correspondence: [email protected] Received: 27 February 2020; Accepted: 19 April 2020; Published: 21 April 2020 Abstract: Limpets are pivotal for structuring and regulating the ecological balance of littoral communities and are widely collected for human consumption and as fishing bait. Limpets of the species Patella caerulea were collected between April 2016 and April 2017 from two sites, and two samplings per each site with varying degree of exposure to wave action and anthropogenic pressure, in Eastern Mediterranean (Pagasitikos Gulf, Central Greece). This study addresses a knowledge gap on population characteristics of P. caerulea populations in Eastern Mediterranean, assesses population structure, allometric relationships, and reproductive status. Morphometric characteristics exhibited spatio-temporal variation. Population density was significantly higher at the exposed site. Spatial relationship between members of the population exhibited clumped pattern of dispersion during spring. Broadcast spawning of the population occurred during summer. Seven dominant age groups were identified, with the dominant cohort in the third-year
    [Show full text]
  • The External Morphology of the Gill of Patella Caerulea L
    D. AKŞİT, B. FALAKALI MUTAF Turk J Zool 2011; 35(4): 603-606 © TÜBİTAK Short Communication doi:10.3906/zoo-0907-82 Th e external morphology of the gill of Patella caerulea L. (Mollusca: Gastropoda) Deniz AKŞİT*, Beria FALAKALI MUTAF Akdeniz University, Faculty of Aquatic Sciences and Fisheries, Dumlupınar Bulvarı, 07059 Antalya - TURKEY Received: 17.07.2009 Abstract: Gross morphology of the gill was studied using a scanning electron microscope (SEM) in order to associate it with its function in genus Patella. Secondary gills consisted of a single layer of gill folds, which appear mostly as triangular leafl ets in scanning electron micrographs. Th e lamellae showed a prominent thickening on the free margin and horizontal swellings, which bear a series of ciliary structures on the frontal surface, as well as disperse bunches. Th ey diff ered from the discocilia and, because chemoreception has been attributed to them in the absence of an osphradium, they are thought to contribute to the primary respiratory role of the gills. Th ese morphological characteristics are to be researched further, both in natural and experimental specimens of this genus. Key words: Patella, gill, SEM, ciliary structures Patella caerulea L. (Mollusca: Gastropoda) solungaçlarının dış morfolojisi Özet: Patella genusunun genel solungaç morfolojisi, fonksiyonuyla ilişkilendirilerek taramalı elekron mikroskobunda incelenmiştir. İkincil solungaç katlantıları taramalı elektron mikroskobunda genellikle üçgenimsi yapraklar şeklinde görülen tek bir tabakadan oluşmaktadır. Herbir yaprağın serbest kenarlarında belirgin bir kalınlaşma ve ön yüzeyinde yatay kabartılarda bir dizi dağınık siller halinde bulunmaktadır. Bu demetler diskosiliadan farklıdır ve kimyasal algılayıcı osfradyumun olmaması nedeniyle, solungaçın birincil görevi solunuma katkıda bulunduğu düşünülmektedir.
    [Show full text]
  • Ferguson Et Al
    UC Irvine UC Irvine Previously Published Works Title Increased seasonality in the Western Mediterranean during the last glacial from limpet shell geochemistry Permalink https://escholarship.org/uc/item/3rf716hg Journal Earth and Planetary Science Letters, 308(3-4) ISSN 0012-821X Authors Ferguson, JE Henderson, GM Fa, DA et al. Publication Date 2011-08-15 DOI 10.1016/j.epsl.2011.05.054 License https://creativecommons.org/licenses/by/4.0/ 4.0 Peer reviewed eScholarship.org Powered by the California Digital Library University of California Earth and Planetary Science Letters 308 (2011) 325–333 Contents lists available at ScienceDirect Earth and Planetary Science Letters journal homepage: www.elsevier.com/locate/epsl Increased seasonality in the Western Mediterranean during the last glacial from limpet shell geochemistry Julie E. Ferguson a,⁎, Gideon M. Henderson a, Darren A. Fa b, J. Clive Finlayson b, Norman R. Charnley a a Department of Earth Sciences, Oxford University, South Parks Road, Oxford, OX1 3AN, UK b The Gibraltar Museum, 18–20 Bomb House Lane, P. O. Box 939, Gibraltar article info abstract Article history: The seasonal cycle is a fundamental aspect of climate, with a significant influence on mean climate and on Received 9 December 2010 human societies. Assessing seasonality in different climate states is therefore important but, outside the Received in revised form 23 April 2011 tropics, very few palaeoclimate records with seasonal resolution exist and there are currently no glacial-age Accepted 31 May 2011 seasonal-resolution sea-surface-temperature (SST) records at mid to high latitudes. Here we show that both Available online 13 July 2011 Mg/Ca and oxygen isotope (δ18O) ratios in modern limpet (Patella) shells record the seasonal range of SST in — Editor: P.
    [Show full text]
  • Patellid Limpets: an Overview of the Biology and Conservation of Keystone Species of the Rocky Shores
    Chapter 4 Patellid Limpets: An Overview of the Biology and Conservation of Keystone Species of the Rocky Shores Paulo Henriques, João Delgado and Ricardo Sousa Additional information is available at the end of the chapter http://dx.doi.org/10.5772/67862 Abstract This work reviews a broad spectrum of subjects associated to Patellid limpets’ biology such as growth, reproduction, and recruitment, also the consequences of commercial exploitation on the stocks and the effects of marine protected areas (MPAs) in the biology and populational dynamics of these intertidal grazers. Knowledge of limpets’ biological traits plays an important role in providing proper background for their effective man- agement. This chapter focuses on determining the effect of biotic and abiotic factors that influence these biological characteristics and associated geographical patterns. Human exploitation of limpets is one of the main causes of disturbance in the intertidal ecosys- tem and has occurred since prehistorical times resulting in direct and indirect alterations in the abundance and size structure of the target populations. The implementation of MPAs has been shown to result in greater biomass, abundance, and size of limpets and to counter other negative anthropogenic effects. However, inefficient planning and lack of surveillance hinder the accomplishment of the conservation purpose of MPAs. Inclusive conservation approaches involving all the stakeholders could guarantee future success of conservation strategies and sustainable exploitation. This review also aims to estab- lish how beneficial MPAs are in enhancing recruitment and yield of adjacent exploited populations. Keywords: Patellidae, limpets, fisheries, MPAs, conservation 1. Introduction The Patellidae are one of the most successful families of gastropods that inhabit the rocky shores from the supratidal to the subtidal, a marine habitat subject to some of the most © 2017 The Author(s).
    [Show full text]