'Photobiont Diversity in Teloschistaceae (Lecanoromycetes)'

Zurich Open Repository and Archive University of Zurich Main Library Strickhofstrasse 39 CH-8057 Zurich www.zora.uzh.ch Year: 2006 Photobiont diversity in Teloschistaceae (Lecanoromycetes) Nyati, Shyam Posted at the Zurich Open Repository and Archive, University of Zurich ZORA URL: https://doi.org/10.5167/uzh-163471 Dissertation Published Version Originally published at: Nyati, Shyam. Photobiont diversity in Teloschistaceae (Lecanoromycetes). 2006, University of Zurich, Faculty of Science. PHOTOBIONT DIVERSITY IN TELOSCHISTACEAE (LECANOROMYCETES) Dissertation zur Erlangung der naturwissenschaftlichen Doktorwürde (Dr. sc. nat) vorgelegt der Mathematisch-naturwissenschaftlichen Fakultät der Universität Zürich von Shyam Nyati aus Indien Promotionskomitee Prof. Dr. Ueli Grossniklaus (Vorsitz) Prof. Dr. Rosmarie Honegger (Leitung der Dissertation) Prof. Dr. Elena Conti Prof. Dr. Martin Grube Zürich, 2006 Table of Contents Table of Contents Zusammenfassung . i-v Summary . ii-vii 1 Introduction . 1-1 1.1 Lichen symbiosis . 1-1 1.1.1 Lichen-forming fungi and their photobionts. 1-1 1.1.2 Specificity and selectivity in lichen symbiosis . 1-2 1.2 Green algal lichen photobionts in focus. 1-3 1.2.1 Green algal taxonomy. 1-3 1.2.2 Trebouxiophyceae and the “lichen algae”. 1-5 1.2.3 The genus Trebouxia: most common and widespread lichen photobionts . 1-6 1.2.4 Trebouxia s.str. and Asterochloris (Tscherm.-Woess) Friedel ined. 1-7 1.3 Aims of the present investigation. .1-9 1.4 References . 1-14 2 Green algal photobiont diversity (Trebouxia spp.) in representatives of Teloschistaceae (Lecanoromycetes, lichen-forming ascomycetes) . 2-1 2.1 Summary . 2-1 2.1.1 Key words: . 2-2 2.2 Introduction . 2-2 2.3 Materials and Methods . 2-5 2.3.1 Lichen collection and storage . 2-5 2.3.2 Photobiont isolation and culture . 2-6 2.3.3 DNA extraction, PCR amplification and Sequencing . 2-14 2.3.4 ITS amplification . 2-14 2.3.5 rbcL amplification . 2-15 2.3.6 Sequencing. 2-15 2.3.7 Phylogenetic analysis . .2-16 2.4 Results. 2-16 2.4.1 Isolation and culturing. 2-16 2.4.2 ITS phylogeny. 2-17 2.4.3 rbcL phylogeny . 2-21 2.5 Discussion . 2-23 2.5.1 Correlations among morphological and molecular data sets . 2-23 2.5.2 Photobionts of the Teloschistaceae . 2-26 2.5.3 Photobionts of the genus Xanthoria . 2-27 2.5.4 Photobionts of Xanthoria parietina s. lat. 2-28 2.5.5 Algal theft by Xanthoria spp. from adjacent Physcia species? . 2-29 2.6 Acknowledgements . 2-31 2.7 References . 2-32 i 3 Novel group I LSU and SSU introns identified in lichen photobionts of the genera Trebouxia and Asterochloris . 3-1 3.1 Abstract . 3-1 3.1.1 Key words: . 3-2 3.2 Introduction . 3-2 3.3 Materials and Methods: . 3-4 3.3.1 Lichen collection, storage and photobiont isolation and culture . 3-4 3.3.2 DNA extraction, PCR amplification and sequencing. 3-8 3.3.3 Sequence alignment and phylogenetic analysis. 3-9 3.3.4 PCR test to confirm presence of 1512 group I introns in photobiont isolates. 3-9 3.4 Results and Discussion . 3-9 3.4.1 Trebouxia LSU 798 group I intron. 3-9 3.4.2 Trebouxia 1512 group I intron. 3-13 3.4.3 LSU and SSU group I intron distribution in Trebouxia and Asterochloris spp. 3-17 3.4.4 Introns of lichen photobionts and mycobionts. 3-18 3.5 Acknowledgements . 3-20 3.6 References . 3-20 4 Genetic diversity among the sterile cultured Trebouxia photobionts from populations of Xanthoria parietina (lichen-forming ascomycete) visualized with RAPD-PCR fingerprinting techniques . 4-1 4.1 Abstract . 4-1 4.1.1 Key words: . 4-2 4.2 Introduction . 4-2 4.3 Materials and methods . 4-4 4.3.1 Specimen collection, photobiont isolation and culturing . 4-4 4.3.2 Genomic DNA isolation. .4-6 4.3.3 ITS amplification, restriction digestion and sequencing . 4-7 4.3.4 ITS sequencing and phylogenetic analysis. 4-7 4.3.5 ITS-RFLP . 4-8 4.3.6 RAPD amplification and fingerprinting analysis . 4-9 4.4 Results. 4-10 4.4.1 Suitability of methods . ..

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