Usbiological Datasheet

D1IP (Neuron-specific Vesicular Protein Calcyon, Caly, Drd1ip, Dopamine Receptor D1 Interacting Protein- Calcyon) Catalog number D9000-05A Supplier United States Biological D1 dopamine receptor-interacting protein calcyon (D1IP) is a type II single transmembrane protein. D1IP belongs to neron-specific gene (NSG) family of proteins. It is required for maximal stimulated calcium release after stimulation of purinergic or muscarinic but not beta-adrenergic receptors. The encoded protein interacts with D1 dopamine receptor drd1) and may interact with other dopamine receptor subtypes and/or GPCRs. Dopamine is an endogenous catecholamine that influences many cellular activities, including behavior, hormone synthesis and release, blood pressure and intracellular ion transport. D1IP (rat 226aa; human 217aa, mouse: 226aa; 24.6kD, mouse chromosome 7F4) D1IP2 is expressed in the pyramidal cells of prefrontal cortex, in hypothalamus and in caudate nucleus. Applications Suitable for use in ELISA, Western Blot. Other applications not tested. Recommended Dilution Western Blot: 1-10ug/ml ELISA: 1:100,000 Optimal dilutions to be determined by the researcher. Storage and Stability Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Immunogen Synthetic peptide corresponding to 16aa of mouse D1IP (Accession #Q9DCA7) (KLH). Formulation Supplied as a lyophilized powder from PBS, 0.05% sodium azide. Purity Purified by immunoaffinity chromatography. Specificity Recognizes mouse D1IP. Product Type Pab Source mouse Isotype IgG Grade Affinity Purified Applications E WB Crossreactivity Mo Storage -20°C Reference 1. Dai R., Bergson C., (2003) Gene 311: 111-117. Powered by TCPDF (www.tcpdf.org).

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A Computational Approach for Defining a Signature of Β-Cell Golgi Stress in Diabetes Mellitus
Page 1 of 781 Diabetes A Computational Approach for Defining a Signature of β-Cell Golgi Stress in Diabetes Mellitus Robert N. Bone1,6,7, Olufunmilola Oyebamiji2, Sayali Talware2, Sharmila Selvaraj2, Preethi Krishnan3,6, Farooq Syed1,6,7, Huanmei Wu2, Carmella Evans-Molina 1,3,4,5,6,7,8* Departments of 1Pediatrics, 3Medicine, 4Anatomy, Cell Biology & Physiology, 5Biochemistry & Molecular Biology, the 6Center for Diabetes & Metabolic Diseases, and the 7Herman B. Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN 46202; 2Department of BioHealth Informatics, Indiana University-Purdue University Indianapolis, Indianapolis, IN, 46202; 8Roudebush VA Medical Center, Indianapolis, IN 46202. *Corresponding Author(s): Carmella Evans-Molina, MD, PhD ([email protected]) Indiana University School of Medicine, 635 Barnhill Drive, MS 2031A, Indianapolis, IN 46202, Telephone: (317) 274-4145, Fax (317) 274-4107 Running Title: Golgi Stress Response in Diabetes Word Count: 4358 Number of Figures: 6 Keywords: Golgi apparatus stress, Islets, β cell, Type 1 diabetes, Type 2 diabetes 1 Diabetes Publish Ahead of Print, published online August 20, 2020 Diabetes Page 2 of 781 ABSTRACT The Golgi apparatus (GA) is an important site of insulin processing and granule maturation, but whether GA organelle dysfunction and GA stress are present in the diabetic β-cell has not been tested. We utilized an informatics-based approach to develop a transcriptional signature of β-cell GA stress using existing RNA sequencing and microarray datasets generated using human islets from donors with diabetes and islets where type 1(T1D) and type 2 diabetes (T2D) had been modeled ex vivo. To narrow our results to GA-specific genes, we applied a filter set of 1,030 genes accepted as GA associated.
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Supplementary Table 3 Complete List of RNA-Sequencing Analysis of Gene Expression Changed by ≥ Tenfold Between Xenograft and Cells Cultured in 10%O2
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Identification of Key Genes and Pathways for Alzheimer's Disease
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