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Proceedings of the 7 Wildlife Assisted Reproductive Technology (ART Proceedings of The 7th Wildlife Assisted Reproductive Technology (ART) Workshop “Reproductive Biotechnology: from Basic to Applications” March 7-11, 2016 the 7th Wildlife Assisted Reproductive Technology (ART) Workshop 2016 Proceedings of The 7th Wildlife Assisted Reproductive Technology (ART) Workshop March 7-11, 2016 the 7th Wildlife Assisted Reproductive Technology (ART) Workshop 2016 Welcome Message By Associate Professor Dr. Chuchat Kamollerd Dean of Faculty of Veterinary Medicine, Khon Kaen University -------------------------------------------------------------------------------------------------------------------------------- Distinguished participants Ladies and Gentlemen. On behalf of the Faculty of Veterinary Medicine, Khon Kaen University, it is my great pleasure to you all a very warm welcome to the 7th Wildlife Assisted Reproductive Technology (ART) Workshop 2016, which is the joint conference of the Zoological Park Organization and Khon Kaen University. The conference is arranged during 7th-11st March 2016, the scientific session held at the Faculty of Veterinary Medicine, Khon Kaen University and the workshop held at Khon Kaen Zoo, Khon Kaen, Thailand. It is a very great opportunity to have the collaboration with the Zoological Park Organization and Khon Kaen Zoo. The conference will dedicate to provide scientific forum focus on wildlife reproduction and ART for scientists, veterinarians, graduate and students to learn and exchange more knowledge. I hope that we all will enjoy the conference and best wish for the successful workshop. I wish that you will have a pleasant time in Khon Kaen. I hope that the joint symposium of Zoological Park Organization and Khon Kaen University will continue for the future and have a strengthen relationship in animal reproductive science and technologies. Thank you very much. the 7th Wildlife Assisted Reproductive Technology (ART) Workshop 2016 Opening Remarks By Mr.Benjapon Nakprasert Director of Zoological Park Organization under the Royal Patronage of H.M. the King ------------------------------------------------------------------------------------------------------------------------------ Distinguished participants Ladies and Gentlemen On behalf of the organizing committee of the 7th Wildlife Assisted Reproductive Technology (ART) Workshop 2016, I am very pleased to welcome colleagues, friends, veterinarians, scientists, students and exhibitors who participating in the joint conference of Khon Kaen University and Zoological Park Organization. This conference is a great occasion for researchers and veterinarians whose works focus for animal reproductive biology to share knowledge and experiences, which in turn will be a great contribution to the wildlife conservation. The conference theme “Reproductive Biotechnology: from Basic to Applications” will offer relevant delegates to have opportunities to review fundamental reproductive science, access novel research finding and technologies for wildlife. The conference features special lectures given by experts in the field and sessions for free communication. The conference also provides a hand on workshop for researchers in the field to practice and share the experience for building up network in future. I wish you have productive scientific forum, successful workshop and enjoy the time during the conference. the 7th Wildlife Assisted Reproductive Technology (ART) Workshop 2016 Organizing committee 1. Benjapon Nakprasert Advisor 2. Assoc Prof.Dr. Chuchart Kamollerd Advisor 3. Sumate Kamolnorranath Advisor 4. Tanachon Kensingh Advisor 5. Dr. Ampika Thongphakdee Chairperson 6. Asst Prof. Sarawut Sringam Vice Chairperson 7. Asst Prof. Dr. Vibuntita Chankitisakul Chairperson of Scientific Committee 8. Chavin Chaisongkarm Venue Committee 9. Nudthakamol Kajornklin Secretary 10. Saifon Yapila Assistant secretary 11. Supalak Kaitsomboon Registration Committee 12. Sunthita Karoon Treasurer 13. Dr.Saksiri Sirisathien Moderator 14. Asst Prof.Dr.Wuttigrai Boonkum Moderator the 7th Wildlife Assisted Reproductive Technology (ART) Workshop 2016 “The 7th Wildlife Assisted Reproductive Technology (ART) Workshop” This workshop is aimed to establish one of the stages where reproductive scientist, veterinarian and relevance persons can join together sharing, talking, and giving their experiences about reproductive science and technology to each others. The dates of the workshop are March 6-11, 2016. It will be held at Khon Kaen University (KKU) and Khon Kaen Zoo (KKZ), Khon Kaen, Thailand. Program of the 7th ART Workshop 2016 6-Mar Participants arrive Khon Kaen Scientific forum at Faculty of Veterinary Medicine, 7-Mar Khon Kaen University 9.00-16.30 Workshop at Khon Kaen Zoo Welcome address: Tanachon Kensingh (Director of KKZ) 8-Mar Overview of ART Workshop: Dr. Ampika Thongphakdee Semen collection and cryopreservation 8.00-16.30 2 Thamin Eld's deer and 2 Hog Deer Embryo collection and cryopreservation 9-Mar 4 Rusa deer 8.00-16.30 Embryo collection and cryopreservation 10-Mar 4 Rusa deer 8.00-16.30 11-Mar Conclusion 8.00-12.00 the 7th Wildlife Assisted Reproductive Technology (ART) Workshop 2016 Agenda of the 7th Wildlife ART Workshop: March 7, 2016 at Faculty of Veterinary Medicine, Khon Kaen University Time Topic Speaker 8.00-9.00 Registration 9.00-9.15 Welcome address Assoc. Prof. Dr. Chuchart Kamollerd (Dean of Khon Kaen University, KKU) Opening remarks Benjapon Nakprasert (Director of Zoological Park Organization, ZPO) Special lecture: 9.15-10.00 Prof.Dr.Mongkol Techakumphu (Vice President of Chulalongkorn University, CU) Reproductive biotechnology: basic to application 10.00-10.15 Group photo & coffee break 10.15-10.45 Why conservation and Wildlife ART in Thailand Sumate Kamolnorranart (DVM) (ZPO) 10.45-11.10 Health practice for captive breeding management Visit Arsaitumkul (DVM) (ZPO) 11.10-11.30 Best practice for wildlife anesthesia and reproductive case Chavin Chaisongkram (DVM) (KKZ, ZPO) 11.30-12.00 The role of oxidative stress in reproduction Dr.Yoswaris Semaming (DVM) (Udon Thani Rajabhat University) 12.00-13.00 Lunch 13.00-13.30 Cryopreservation of gametes and embryos: fundamental aspects Asst Prof. Dr. Theerawat Tharasanit (DVM) (CU) 13.30-14.00 Cryopreservation of sambar deer semen Assoc Prof. Dr. Thevin Vongpralub (KKU) 14.00-14.30 Semen evaluation and improvement of semen quality Asst Prof. Sarawut Sringam (DVM) (KKU) 14.30-14.50 Coffee break Preliminary study on superovulation in rusa deer using a split- 14.50-15.20 single intramuscular administration of follicle-stimulating Asst. Prof. Dr. Vibuntita Chankitisakul (DVM) (KKU) hormone Prof. Dr. Yan-Der Hsuuw (DVM) (National Pingtung University of Science and 15.20-15.50 Embryo development and quality assessment Technology) 15.50-16.20 Embryo cryopreservation: slow freezing aspect Dr. Saksiri Sirisathien (DVM) (KKU) 16.30-16.40 Closing scientific session 16.40-17.10 Introduction for workshop (only participants for workshop) 18.00-20.00 Welcome dinner and transport to Khon Kaen Zoo the 7th Wildlife Assisted Reproductive Technology (ART) Workshop 2016 1 Special Lecture: Reproductive Biotechnology: Basic to Application Mongkol Techakumphu Department of Obstetrics Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand, 10300 the 7th Wildlife Assisted Reproductive Technology (ART) Workshop 2016 2 Why Conservation and Wildlife ART in Thailand Sumate Kamolnorranart Bureau of Conservation and Research, the Zoological Park Organization, Thailand Increasingly altered and fragmented habitats directly affected natural wildlife populations. More species are actively managed for their long-term survival. Conservation breeding of endangered species is one of the crucial missions at the ZPO. Today, total of 532 species (10,280 individuals); 3,971 individuals of mammal, 4,961 individuals of birds, 1,329 individuals of reptiles and 19 individuals amphibians are cared in ZPO zoos. Seven member zoos under the Zoological Park Organization (ZPO) are implementing ‘UN Decade on Biodiversity’ campaign by contributing to specific strategies including; 1) maintaining genetic diversity of captive wildlife population of global importance; 2) strengthen health management, research programs on reproductive science and establish genome bank; and 3) reintroduction programs for the ‘extinct-in-the-wild’ species. Prioritized species selected for IMP program are highlighted on Thai native endangered species such as Eld’s deer (Rucervus eldii), Eastern Sarus crane (Grus antigone sharpii), Malayan tapir (Tapirus indicus), goral (Naemorhedus griseus) and wild cats. The presentation will give the information “why conservation and biodiversity are important?” and “update assisted reproductive technology in wildlife in Thailand”. the 7th Wildlife Assisted Reproductive Technology (ART) Workshop 2016 3 Health Practice for Captive Breeding Management Visit Arsaithamkul Bureau of Conservation and Research, the Zoological Park Organization, Thailand Wildlife captive breeding is a part of wildlife conservation, wildlife health is crucial for wildlife conservation. The vital role of play in conservation initiatives have to consider management ex situ wild animal relate to in situ wild animal. Zoo veterinarians and zoo animal managing staffs have been challenged to apply management, welfare and animal health not only for individuals but have more in preventive and therapeutic medicine for populations. Working group on each specific species have to design as Scientific Advisory
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