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BIOLOGICAL PROPERTIES and CHEMICAL DIVERSITY of Sinularia Flexibilis, an ALCYONACEAN SOFT CORAL

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BIOLOGICAL PROPERTIES and CHEMICAL DIVERSITY of Sinularia Flexibilis, an ALCYONACEAN SOFT CORAL Journal of Sustainability Science and Management ISSN: 1823-8556 Volume 13 Number 2, December 2018: 15-33 © Penerbit UMT BIOLOGICAL PROPERTIES AND CHEMICAL DIVERSITY OF Sinularia flexibilis, AN ALCYONACEAN SOFT CORAL THILAHGAVANI NAGAPPAN1.2* AND KISHNETH PALANIVELOO3 1 School of Marine and Environmental Sciences, Unversiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia 2Institute of Marine Biotechnology, Unversiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia 3Institute of Ocean and Earth Science, Universiti Malaya, Jalan Universiti, 50603 Kuala Lumpur, Malaysia *Corresponding author: [email protected] Abstract: Soft corals of the genus Sinularia (order Alcyonacea) are one of the most widespread sessile organisms in the Indo-Pacific waters. Unlike the hard corals, the major portion of soft coral is made up of inorganic skeletons consisting of calcareous spicules surrounded by a thin layer of tissue. This layer of tissue, made up of fleshy colonies with organic matter, is responsible in diversity of secondary metabolites. In order to survive the outburst of algal bloom, dynamic of microbial growth and predation of marine organisms, these soft corals synthesize unique chemicals as part of defense mechanism that makes them unpalatable to most marine life. As such, members of the genus Sinularia exhibits a diversity of secondary metabolites ranging from sesquiterpenes, diterpenes, polyhydroxylated steroids, and polyamine compounds. These metabolites had been shown to possess various biological activities such as antimicrobial, anti-inflammatory and cytotoxicity. This present paper reviews the chemistry and highlights the potential biological activities of metabolites from Sinularia flexibilis and provides a perspective for future research. Keywords: Sinularia flexibilis, soft coral, secondary metabolites, biological activities. Introduction 2012). Researchers from Australia, India and Japan have isolated and elucidated an extensive The status of marine natural products has been a range of secondary metabolites constituting popular subject for reviews (Kelecom, 2002; sesquiterpenes, diterpenes, polyhydroxylated Proksch et al., 2002; Haefner, 2003; Capon, 2010; steroids and polyamine skeletons. Metabolites Blunt et al., 2015) as this field has resulted in the isolated from the genus Sinularia is reported to development of a substantial number of bioactive display potential bioactivities such as metabolites for the betterment of mankind. antimicrobial, anti-inflammatory and cytotoxic Scheuer (1983) and Faulkner (2002) contributed activities closely related to the high interaction tremendously in creating a platform in the field of in the marine environment (Palaniveloo marine natural product chemistry through & Vairappan, 2014). To date, numerous comprehensive reviews pertaining to chemical publications are currently available pertaining to and biological perspectives of secondary the chemistry and pharmacology of metabolites metabolites. The coral reef represents an isolated from Sinularia flexibilis found in the extraordinary diverse ecosystem in tropical waters of Australia, Japan and India (Kamel et al., environments with soft corals often constituting a 2005; Chen et al., 2010; Anjeneyulu et al., 1997) dominant part of the reef. Sinularia is a genus in as well as extensive research on S. flexibilis from phylum Cnidaria classified in class Alcyonaria, in the Formosan waters (Hu et al., 2013; Duh et al., the family Alcyoniidae and is widely distributed 1998; Lin et al., 2009; 2013; Lo et al., 2009; from the waters of east Africa to the western 2010) is documented. Over the past 30 years, Pacific, inhabiting the coral reefs or rocks up to more than 15 000 novel secondary metabolites depths of 30 meters (Piccinetti et al., 2017). This have been discovered from this organism (Li et genus is known to consist approximately 90 al., 2006). Since 1995, various research papers species, of which more than 50 have been have been published on investigations of the chemically evaluated (Chen et al., chemical constituents 16 BIOLOGICAL PROPERTIES AND CHEMICAL DIVERSITY OF Sinularia flexibilis, AN ALCYONACEAN SOFT CORAL of the soft coral genus Sinularia reporting new, activities of the chemical constituents from S. novel terpenoids and their pharmacological flexibilis (Fig. 1) which has been a subject of potentials, globally. Hence, this comprehensive interest in the area of marine drug discovery. review focuses on the chemistry and biological Figure 1: Underwater photographs of S. flexibilis found in the waters of Mantanani Island, Sabah, Malaysia. Terpenoids of Alcyonacean and only sesquiterpenes were isolated from Parerythropodium spp. In the family Investigations involving soft corals started as Nephtheiidae, the genus Nephthea spp comprised early as in 1972 with the isolation of of sesquiterpenes or diterpenes of which 70% prostaglandin [(15R)-PGA2] from Plexaura are cembrane-type skeleton producers. homomalla Esper, a gorgonian whereas the Interestingly, Litophyton spp have only been attention on isolation of secondary metabolites reported to contain cembranoid-type diterpenes from Alcyonacean started in 1970s. Coll (1992) while the genera Lemnalia, Paralemnalia, and reported patterns in chemical distribution across Capnella spp produces sesquiterpenes. Across the various soft coral genera where terpenoids the family Xeniidae, the genera Efflatounaria, have been confirmed as the dominating cluster of Cespitularia and Xenia, Anthelia spp have been secondary metabolites in Alcyonacean. studied with the earlier two genera producing diterpenes, sesquiterpenes and cembrane-type According to Coll (1992), the presence of diterpene. In the latter two genera, 90% of the diterpenes in Sinularia spp and Sarcophyton compounds isolated from Xenia spp and spp is made up of cembrane-type skeleton Anthelia spp belong to the xenicane-type while Lobophytum spp was reported to produce skeleton group. Shown below in Figure 2 (1- a mixture of 70 % cembrane and 30% 19) is the highlight of diverse skeletal pattern of germacrene-type skeleton, respectively. across soft coral genera. The Cladiella spp was found to produce cladiellane-type, cyclized cembrane compounds Journal of Sustainability Science and Management Volume 13 Number 2, December 2018 : 15-33 Thilahgavani Nagappan & Kishneth Palaniveloo 17 Figure 2: Diversity of isolated chemical skeletal across soft corals genera. Chemical diversity of Sinularia flexibilis al., 2008; Lakshmi et al., 2009; Chen et al., 2010; Hu et al., 2013; Lin et al., 2013; 2009; Genus Sinularia is placed under the soft coral Chen et al., 2012) making them valuable for family Alcyoniidae. In total, 90 species of biomedical research. The secondary Sinularia have been identified worldwide and metabolites isolated from soft corals, apart close to 50 species have been chemically studied from having biomedical potentials, it is also (Chen et al., 2012). Numerous publications serves as chemotaxonomical markers for currently exists pertaining to this genus from Sinularia species identification (Veseveldt, Australian, Japanese and Indian waters (Coll et 1980; Palaniveloo & Vairappan, 2014). al., 1985; Anjeneyulu et al., 1997; Kamel et al., 2005). The secondary metabolites isolated Sesquiterpenes commonly includes sesquiterpenes, diterpenes, polyhydroxylated steroids and polyamine which Among earliest sesquiterpenes discovered from displays a wide array of biological properties such Sinularia was furanosesquiterpenoid acid (20) by as antimicrobial, anti-inflammatory and cytotoxic Coll et al. and co-workers (1977) from Sinularia activities (Anjeneyulu et al., 1997; Duh et al., gonatodes followed by isolation of furanoquinol 1998; Kamel et al., 2005; Khalesi et (21) from Sinularia lochomodes in 1978. Journal of Sustainability Science and Management Volume 13 Number 2, December 2018 : 15-33 18 BIOLOGICAL PROPERTIES AND CHEMICAL DIVERSITY OF Sinularia flexibilis, AN ALCYONACEAN SOFT CORAL Furanosesquiterpenoids are commonly known to waters of Okinawa, Japan (Kusumi et al., exhibit a wide range of biological potentials such 1992). This same sesquiterpene skeleton, was as anti-microbial, anti-inflammatory and cytotoxic initially isolated from the soft coral genus activities (Kamel et al.,2005; Chen et al., 2010; Lobophytum by Dunlop and Well (1979) and Yang et al., 2013). However, only one furano- Sinularia dissecta (Reddy et al., 1993; Ramesh type sesquiterpene have been reported from S. et al., 1999; Reddy et al., 2002). Figure 3 flexibilis to date. The compound displays the chemical structures of (+)-β-elemene (22) was isolated along with the furanosesquiterpenoid acid (20), furanoquinol diterpene lobatriene from S. flexibilis from (21) and (+)-β-elemene (22). Figure 3: The structures of furanosesquiterpenoid acid (20), furanoquinol (21) and (+)-β- elemene (22) from the genus Sinularia. Diterpenes an interesting oxygen moiety bearing hydroxyl group (-OOH) represented by a low field Cembranes are the most common secondary chemical shifts of δ 7.60 – 7.80 ppm. Lo and metabolites isolated from soft corals. This class of co-workers also isolated more cembranoid-type diterpenes possesses a 14-membered ring diterpenes, sinuladiterpenes G (35) - I (37) skeleton. As such, cembranes can be considered a (Fig. 4) as a continuation of their previous work chemotaxonomical marker for soft corals. The documented in 2009 (Lo et al., 2010). earliest of cembrane-type skeleton isolated is pukalide (23), consisting of a
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