Analysis of Mono Nucleotides in Presence of Inosine and Hypoxanthine

Analysis of mono nucleotides in presence of inosine and hypoxanthine

Method: Matrix Application-No.:

HPLC 111270

Substances: hypoxanthine; ump; uridine monophosphate; imp; inosine monophosphate; inosine; amp; adenosine monophosphate; adp; adenosine diphosphate; atp; adenosine triphosphate

Column: CC 250/4 NUCLEOSIL 100-5 C18

Phase: NUCLEOSIL 100-5 C18

MN catalog number:

Sample Analysing meat the problem of separating inosine and hypoxanthine pretreatment: in the presence of different mono nucleotides occurs. With the column material NUCLEOSIL 100-5 C18 these compounds can easily be quantified. The retention time of hypoxanthine and inosine monophosphate depends on how long the column is regenerated. A regeneration time of 13-15 min. yields in good separations whereas regeneration times longer than 25 min. leads to overlapping of the peaks of hypoxanthine and UMP.

Conditions: Eluents: A) 0.1 M KH2PO4, 4 mM tetrabutylammonium dihydrogen phosphate, pH 6.0 B) 50% A plus 50% methanol Gradient: 0-6 min. 15% B, 6-16 min. from 15% to 90% B, 16-17 min. 90% B, 17-17.5 min. to 15% B, 17.5-31.5 min. 15% B, 32 min.: start of next run Flow rate: 0.5 mL/min

地址：上海市闵行区沁春路1366弄38号501室电话：021-54424306 021-54424445 021-58742608 传真：021-58742609 网址：www.truelab.cn 邮编：201108 - 1 -

Create PDF with GO2PDF for free, if you wish to remove this line, click here to buy Virtual PDF Printer Detection Subsequent analysis:

Author(-s): HPLC department

Source: MACHEREY-NAGEL

Keywords: nucleotides; inosine; hypoxanthine

Chromatogram:

地址：上海市闵行区沁春路1366弄38号501室电话：021-54424306 021-54424445 021-58742608 传真：021-58742609 网址：www.truelab.cn 邮编：201108 - 2 -

Create PDF with GO2PDF for free, if you wish to remove this line, click here to buy Virtual PDF Printer Legend: 1) Hypoxanthine 2) UMP (Uridine monophosphate) 3) IMP (Inosine monophosphate) 4) Inosine 5) AMP (Adenosine monophosphate) 6) ADP (Adenosine diphosphate) 7) ATP (Adenosine triphosphate)

地址：上海市闵行区沁春路1366弄38号501室电话：021-54424306 021-54424445 021-58742608 传真：021-58742609 网址：www.truelab.cn 邮编：201108 - 3 -

Create PDF with GO2PDF for free, if you wish to remove this line, click here to buy Virtual PDF Printer