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Rnascope® Probes for Stem Cells Research Get Probes for Your Gene of Interest Designed and Manufactured in Two Weeks Probe List Series Stem Cell Research RNAscope® Probes for Stem Cells Research Get probes for your gene of interest designed and manufactured in two weeks. Featured Publications using Detection of Stem Cell Markers gene of interest, as for example long non-coding RNAscope® Technology RNA. The RNAscope® assay allows you to visualize The rapid and expansive field of stem cell biology and quantify virtually any gene from any genome in Wnts produced by Osterix- has demonstrated the remarkable capabilities any tissue. expressing osteolineage cells of these cells to self-renew, differentiate, and regulate their proliferation and reprogram. Yet the field is still burgeoning with differentiation. studies trying to elucidate stem cell populations, Tan SH. et. al. Proc Natl Acad Sci USA. 2014. Dec 9;111(49): characterize stem cell markers, and identify the ® E5262-71.PMID: 25422448 signals secreted from stem cells. RNAscope in situ hybridization (ISH) technology enables cell- Characterization of LGR5 stem specific localization of RNA transcripts quickly cells in colorectal adenomas and and precisely for markers of stem cell populations. carcinomas. The RNAscope® assay can be used to: Baker AM. et. al. Sci Rep. 2015 Mar 2;5:8654.PMID: 25728748 • Identify, characterize, and locate stem PMID: 25728748 cell populations • Reveal markers of stem cell maintenance Pontin functions as an essential coactivator for Oct4-dependent and regeneration lincRNA expression in mouse • Identify long non-coding RNAs in stem cells FIGURE 1. Detection of TP63 mRNA expression in embryonic stem cells. ® • Detect stem cell markers when no reliable human prostate FFPE tissue using RNAscope 2.0 HD Boo K. et. al. Nat Commun. 2015 Detection Kit - RED Apr 10;6:6810. PMID: 25857206 antibodies are available RNAscope® ISH technology provides a unique See more publications at detection method for stem cell markers that is acdbio.com/publications both highly sensitive and specific for detection of any gene, especially low abundance targets. And not only can RNAscope® technology be used to discern if your gene of interest is in a stem cell, lineage progenitor, or differentiated cell, but it can also show its sub cellular localization. The multiplexing capabilities of the RNAscope® assay allows detection of multiple markers at once, enabling exquisite characterization of stem cell populations as well as detection of both stem cell markers and the signals they produce. RNAscope® technology also helps solve the problem when no FIGURE 2. Detection of MMP9 mRNA expression in human lung FFPE tissue using the RNAscope® 2.0 HD Detection Kit - RED reliable antibodies or no antibodies exist for your Visualize your stem cell RNA today www.acdbio.com/stemcells RNAscope® for Human Stem Cells Research Probes are also available in mouse, rat and many other species. Learn more at www.acdbio.com/probesearch Catalog No. Probe Name Catalog No. Probe Name Catalog No. Probe Name Catalog No. Probe Name 401191 Hs-ABCB1 418741 Hs-ERBB2-no-X-Mm 407671 Hs-KITLG 419841 Hs-PTPN11 422721 Hs-ABCG2 310301 Hs-ESR1 419601 Hs-KMT2A 444331 Hs-RARA 419211 Hs-AKT1 491521 Hs-ETV4 311021 Hs-LGR5 419871 Hs-RB1 310151 Hs-ALDH1A1 405491 Hs-EZH2 445721 Hs-LIF 417871 Hs-REST 418321 Hs-APP 312111 Hs-FGF2 588991 Hs-LIN28A 419901 Hs-RUNX1 419271 Hs-ATM 439421 Hs-FGF2-O1 408991 Hs-MAPT 440071 Hs-RUNX2 591721 Hs-BCL2 419491 Hs-FLT3 601731 Hs-MCAM 600951 Hs-SHH 319931 Hs-BCR 418471 Hs-FOXP3 423081 Hs-MET-E11-E20 429781 Hs-SIRT1 591931 Hs-BMI1 310991 Hs-GLI1 423061 Hs-MET-E2 400871 Hs-SOX2 485471 Hs-BRCA1 310081 Hs-HER2 423091 Hs-MET-E21 404221 Hs-SOX9 436981 Hs-BRCA1-E11 310761 Hs-HGF 423071 Hs-MET-E3-E10 431031 Hs-SOX9-O1 436561 Hs-CASP3 441421 Hs-HGF-No-XMm 423101 Hs-MET-FL 425631 Hs-STAT3 591821 Hs-CCND1 435361 Hs-HGF-O1 443451 Hs-MET-no-XMm 414801 Hs-TEK 601501 Hs-CCR5 418701 Hs-HGF-v2d 408011 Hs-MMP14 605511 Hs-TERT 313021 Hs-CD24 605221 Hs-HIF1A 311331 Hs-MMP9 400881 Hs-TGFB1 560821 Hs-CD34 314331 Hs-HIF1A-3UTR 313811 Hs-MMP9-30LE 407121 Hs-TGFB1-long 311271 Hs-CD44 428351 Hs-HLA-DRB1 444751 Hs-MSI1 443481 Hs-TGFB1-no-XMm 419351 Hs-CDK12 426691 Hs-HLA-G 419681 Hs-MTOR 439761 Hs-THPO 311401 Hs-CDKN1A 319851 Hs-HMOX1 310391 Hs-MUC1 311281 Hs-TLR4 310181 Hs-CDKN2A 402951 Hs-ICAM1 311761 Hs-MYC 310421 Hs-TNF-a 447331 Hs-CDKN2A-No-XMm 310501 Hs-IFN-G 447281 Hs-MYC-No-XMm 411841 Hs-TNFSF10 310051 Hs-CMET 559781 Hs-IFNG 604491 Hs-NANOG 431641 Hs-TNFSF10-O1 425301 Hs-CSF3 313031 Hs-IGF1 421461 Hs-NCAM1 605201 Hs-TP53 554341 Hs-CTLA4 314641 Hs-IGF1-tv1 406331 Hs-NGFR 601891 Hs-TP63 311731 Hs-CTNNB1 602051 Hs-IL10 413481 Hs-NOD2 310431 Hs-TWIST 422991 Hs-CXCL12 316061 Hs-IL10-sense 416541 Hs-NODAL 440371 Hs-VCAM1 310511 Hs-CXCR4 310931 Hs-IL17A 311861 Hs-NOTCH1 311351 Hs-VEGF 421411 Hs-DKK1 310361 Hs-IL1B 419751 Hs-NPM1-X-CLEC2D 423161 Hs-VEGFA 310061 Hs-EGFR 402041 Hs-IL2 588881 Hs-PAX6 412011 Hs-VEGFA-noXrodent 319461 Hs-EGFR-delta 542211 Hs-IL3 604481 Hs-PDGFRA 312121 Hs-VEGFR2 418731 Hs-EGFR-no-X-Mm 315191 Hs-IL4 548991 Hs-PDGFRB 429421 Hs-WNT1 438311 Hs-EGFR-tv1 310371 Hs-IL6 592861 Hs-POU5F1 429431 Hs-WNT3A 428381 Hs-EGFR-tv4 559021 Hs-ITGA6 441691 Hs-PPARG 604921 Hs-WNT5A 410591 Hs-EPAS1 546181 Hs-JAG1 311261 Hs-PROM1 415581 Hs-WT1 410601 Hs-EPAS1-sense 403791 Hs-JAK2 417531 Hs-PSCA 310281 Hs-EPCAM 591611 Hs-KDR 310121 Hs-PTEN 312671 Hs-ERBB2-FL 435371 Hs-KDR-O1 408511 Hs-PTEN-3UTR 411411 Hs-ERBB2-LE1 606401 Hs-KIT 406801 Hs-PTGS2 ACD offers an ever-growing selection of RNA ISH probes. Don’t see your gene of interest? RNAscope® Made-to-Order Target Probes designed within 2 weeks for virtually ANY gene from ANY species in ANY tissue. Learn more at www.acdbio.com/target-probes-made-order Visualize your stem cell RNA today www.acdbio.com/stemcells For Research Use Only. Not for diagnostic use. RNAscope is a registered trademark of Advanced Cell Diagnostics, Inc. in the United States or other countries. All rights reserved. California, USA ©2016 Advanced Cell Diagnostics, Inc. Doc #: MK 51-015/Rev A/Effective date 04152016.
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