Genetically Encoded Calcium Indicators for Multi-Color Neural Activity Imaging and Combination with Optogenetics
ORIGINAL RESEARCH ARTICLE published: 04 March 2013 MOLECULAR NEUROSCIENCE doi: 10.3389/fnmol.2013.00002 Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics Jasper Akerboom 1, Nicole Carreras Calderón 1,2,3, Lin Tian 1†, Sebastian Wabnig 4, Matthias Prigge 5, Johan Tolö 6, Andrew Gordus 7, Michael B. Orger 8,9, Kristen E. Severi 8, John J. Macklin 1, Ronak Patel 1, Stefan R. Pulver 1, Trevor J. Wardill 1†, Elisabeth Fischer 4, Christina Schüler 4,Tsai-WenChen1, Karen S. Sarkisyan 1, Jonathan S. Marvin 1, Cornelia I. Bargmann 7, Douglas S. Kim 1, Sebastian Kügler 6, Leon Lagnado 2, Peter Hegemann 5, Alexander Gottschalk 4, Eric R. Schreiter 1,3 and Loren L. Looger1* 1 Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA 2 Medical Research Council Laboratory of Molecular Biology, Cambridge, UK 3 Department of Chemistry, University of Puerto Rico - Río Piedras, San Juan, PR, USA 4 Institute of Biochemistry and Buchmann Institute for Molecular Life Sciences, Johann Wolfgang Goethe-University Frankfurt, Frankfurt, Germany 5 Experimentelle Biophysik, Humboldt Universität zu Berlin, Berlin, Germany 6 Department of Neurology, University Medicine Göttingen, Göttingen, Germany 7 Laboratory of Neural Circuits and Behavior, Howard Hughes Medical Institute, The Rockefeller University, New York, NY, USA 8 Department of Molecular and Cellular Biology, Center for Brain Science, Harvard University, Cambridge, MA, USA 9 Champalimaud Neuroscience Programme, Champalimaud Centre for the Unknown, Lisboa, Portugal Edited by: Genetically encoded calcium indicators (GECIs) are powerful tools for systems Piotr Bregestovski, Institut national neuroscience. Here we describe red, single-wavelength GECIs, “RCaMPs,” engineered de la santé et de la recherche from circular permutation of the thermostable red fluorescent protein mRuby.
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