Title: Preprint supplement to: “Phylogenetic inference identifies two eumetazoan TRPM clades and an 8th family of TRP channel, TRP soromelastatin (TRPS)”
Authors and Affiliations: Nathaniel J. Himmel1,*, Thomas R. Gray1, and Daniel N. Cox1,*
1 – Neuroscience Institute, Georgia State University, Atlanta, GA, 30303 * - Corresponding authors
Authors for Correspondence:
Nathaniel J. Himmel Daniel N. Cox Neuroscience Institute Neuroscience Institute Georgia State University Georgia State University P.O. Box 5030 P.O. Box 5030 Atlanta, GA 30302-5030 Atlanta, GA 30302-5030 [email protected] [email protected] 1 Supplementary Materials: 2 Phylum Species Source Cnidaria Aurelia (moon jelly) (Gold, et al. 2019) Acropora digitifera (coral) (Shinzato, et al. 2011) Acropora tenuis (coral) (Voolstra, et al. 2015) Aiptasia (sea anemone) (Baumgarten, et al. 2015) Amplexidiscus fenestrafer (elephant ear anemone) (Wang, et al. 2017) Fungia spp. (coral) (Voolstra, et al. 2015) Galaxea fascicularis (galaxy coral) (Voolstra, et al. 2015) Goniastrea aspera (stony coral) (Voolstra, et al. 2015) Pocillopora damicornis (lace coral) (Cunning, et al. 2018) Porites lutea (small polyp stony coral) (Voolstra, et al. 2015) Stylophora pistillata (hood coral) (Voolstra, et al. 2017) Xenacoelomorpha Hofstenia miamia (three-banded panther worm) (Gehrke, et al. 2019) Praesagittifera naikaiensis (acoel flatworm) (Arimoto, et al. 2019) Hemichordata Ptychodera flava (Simakov, et al. 2015) Chordata Petromyzon marinus (sea lamprey) (Smith, et al. 2013) Eptatretus burger (inshore hagfish) PRJEB21290 Chiloscyllium punctatum (brownband bamboo (Hara, et al. 2018) shark) Rhincodon typus (whale shark) (Hara, et al. 2018) Scyliorhinus torazame (cloudy catshark) (Hara, et al. 2018) Carcharodon carcharias (white shark) (Marra, et al. 2019) Nemertea Notospermus geniculatus (ribbon worm) (Luo, et al. 2018) Phoronida Phoronis australis (horseshoe worm) (Luo, et al. 2018) 3 Table S1. Genomes added to the initial NCBI-based sequence database. 4 1 Supplemental Figures
2 Fig. S1. TRPS constitutes a distinct family of TRP channel. Maximum likelihood tree for 3 TRPM, TRPS (ced-11-like), TRPN, and TRPC sequences, for all those species in initial database 4 that had a ced-11-like sequence. UFboot confidence is indicated by red-green color scale, with 5 major branch values listed.
6 Fig. S2. Maximum likelihood tree for TRPM, TRPS (ced-11-like), TRPN, and TRPC sequences 7 for all those species in initial database that had a ced-11-like sequence, but excluding 8 Xenacoelomorpha and Cnidaria in order to test for effects of long-branch attraction. UFboot 9 confidence is indicated by red-green color scale, with major branch values listed.
10 Fig. S3. Graph Splitting tree for TRPM, TRPS (ced-11-like), TRPN, and TRPC sequences for all 11 those species in initial database that had a ced-11-like sequence, in order to test for effects of 12 long-branch attraction. Edge perturbation (EP) confidence is indicated by red-green color scale, 13 with major branch values listed.
14 Fig. S4. Reconciled and rearranged maximum likelihood TRPS phylogram with duplication sites 15 (red) and UFboot branch support values listed. Branches without support values were rearranged 16 (<95 UFboot) by NOTUNG. Individual expansion events occurred in molluscs, nematodes, 17 tardigrades, and chelicerates. While S. maritima has 2 TRPS genes, this was not assumed to 18 represent a taxon-wide duplication event due to it being the sole representative of Myriapoda.
19 Fig. S5. Two hypotheses concerning the loss of TRPS in Ambulacraria and Olfactores. While a 20 monophyletic Deuterostomia has been well supported for some time (left), recent work has 21 suggested that Ambulacraria may be a sister clade to Xenacoelomorpha (Philippe, et al. 2019) 22 (right).
23 Fig. S6. Arthropod TRPS diversification, extracted from Fig. S3. The duplication in arthropod 24 TRPS appears restricted to Chelicerata, and the simplest hypothesis concerning TRPS loss is that 25 it was lost early in the evolution of Pancrustacea.
26 Fig. S7. Reconciled and rearranged maximum likelihood tree of TRPM sequences from 27 Ambulacraria (bold), with TRPM sequences from Cnidaria, Xenacoelomorpha, human, and 28 Drosophila for context, and TRPS sequences for rooting.
29 Fig. S8. Reconciled and rearranged maximum likelihood tree of TRPM sequences from Chordata 30 (bold, excluding ray-finned fish), with TRPM sequences from Cnidaria, Xenacoelomorpha, 31 human, and Drosophila for context, and TRPS sequences for rooting.
32 Fig. S9. Reconciled and rearranged maximum likelihood tree of TRPM sequences from 33 Lophotrochozoa (bold), with TRPM sequences from Cnidaria, Xenacoelomorpha, human, and 34 Drosophila for context, and TRPS sequences for rooting.
35 Fig. S10. Reconciled and rearranged maximum likelihood tree of TRPM sequences from 36 Priapulida and Nematoda (bold), with TRPM sequences from Cnidaria, Xenacoelomorpha, 37 human, and Drosophila for context, and TRPS sequences for rooting. 1 Fig. S11. Reconciled and rearranged maximum likelihood tree of TRPM sequences from 2 Priapulida and Arthropoda (bold), with TRPM sequences from Cnidaria, Xenacoelomorpha, 3 human, and Drosophila for context, and TRPS sequences for rooting.
4 Fig. S12. Reconciled and rearranged maximum likelihood tree of TRPM sequences from 5 Ambulacraria (bold) as in Fig. S7, with Xenacoelomorpha removed. Ambulacrarians have both 6 α- and βTRPMs, and saw independent expansion of βTRPM.
7 Fig. S13. Reconciled and rearranged maximum likelihood tree of TRPM sequences from 8 Chordata (bold, excluding ray-finned fish) as in Fig. S8, with Xenacoelomorpha. Chordates 9 have α- and βTRPMs, and both α- and βTRPMs expanded in vertebrates.
10 Fig. S14. Reconciled and rearranged maximum likelihood tree of TRPM sequences from 11 Lophotrochozoa (bold) as in Fig. S9, with Xenacoelomorpha removed. Lophotrochozoans have 12 both α- and βTRPMs, and saw independent expansion of βTRPM.
13 Fig. S15. Reconciled and rearranged maximum likelihood tree of TRPM sequences from 14 Priapulida and Nematoda (bold) as in Fig. S10, with Xenacoelomorpha removed. Nematodes 15 likely have both α- and βTRPMs, and likely saw expansion in both. However, given that 16 Toxocara canis TRPMs are the only members of the nematode αTRPM clade, it is unclear if the 17 α expansion was species-specific.
18 Fig. S16. Reconciled and rearranged maximum likelihood tree of TRPM sequences from 19 Priapulida and Arthropoda (bold) as in Fig. S11, with Xenacoelomorpha removed. The majority 20 of Arthropods only have αTRPM (many having only a single copy), but several chelicerates and 21 crustaceans may have channels more distantly related to human, priapulid, and cnidarian 22 βTRPMs.
23 Fig. S17. Vertebrate TRPM8 was independently lost in most vertebrate lineages, surviving only 24 in the lobe-finned fish lineage (including tetrapods). Maximum likelihood tree of TRPM 25 sequences from Chordata (including lancelets, tunicates, agnathans, sharks, coelecanth, 26 tetrapods, and ray-finned fish), rooted in TRPS, with the 8 vertebrate TRPM clades labeled. 27 Unlabeled clades are from invertebrate species. UFboot confidence is indicated by red-green 28 color scale. Black indicates rearranged branches (<95 UFBoot). 29 Tree scale: 1
TRP Family TRPS (soromelastatin)
TRPM (melastatin)
TRPN (no mechanoreceptor potential C) TRPC (canonical)
Hofstenia miamia|Three-banded panther worm|scaffold387 211324 251000 F Hofstenia miamia|Three-banded panther worm|scaffold387 254022 257456 F Hofstenia miamia|Three-banded panther worm|scaffold387 219858 223314 F
Hofstenia miamia|Three-banded panther worm|scaffold387 247539 251000 F
Hofstenia miamia|Three-banded panther worm|scaffold387 196851 200279 F
Hofstenia miamia|Three-banded panther worm|scaffold18 807971 810976 F
Hofstenia miamia|Three-banded panther worm|scaffold488 5372 8923 F Hofstenia miamia|Three-banded panther worm|scaffold112 437721 469571 R Aurelia|Moon jelly|Seg1803.3 transcript id Seg1803.3|GoldUCD|mRNA.D3Y31Hofstenia miamia|Three-banded panther worm|scaffold18 866412 869501 F
Praesagittifera naikaiensis|Acoel flatworm|g16021.t1
Praesagittifera naikaiensis|Acoel flatworm|g11490.t1
Praesagittifera naikaiensis|Acoel flatworm|g36760.t1 Parasteatoda tepidariorum|American house spider|XP 015907052 Praesagittifera naikaiensis|Acoel flatworm|g4935.t1
Mizuhopecten yessoensis|Yesso scallop|XP 021378397.1
Crassostrea virginica|Eastern oyster|XP 022340640.1
Limulus polyphemus|American horseshoe crab|XP 022255565 Pomacea canaliculata|Golden apple snail|PVD30001.1
Aplysia californica|California sea hare|XP 005094347.1 Crassostrea gigas|Pacific Oyster|XP 019919132.1
Stylophora pistillata|Hood coral|Spis14035.t1 SpisGene14035 Araneus ventricosus|Orb-weaver spider|GBM10676
Pocillopora damicornis|Lace coral| pdam 00007420-RA
Limulus polyphemus|American horseshoe crab|XP 022254350
Limulus polyphemus|American horseshoe crab|XP 022247894 Goniastrea aspera|Stony coral|gasp1.m3.6245.m1 Mizuhopecten yessoensisXP 021352500.1 Parasteatoda tepidariorum|AmericanLimulus polyphemus|American house spider|XP horseshoe 015912591 crab|XPGalaxea 022250999 fascicularis|Galaxy coral|gfas1.m1.11287.m1
Centruroides sculpturatus|Arizona bark scorpion|XP 023241337
Galendromus occidentalis|Western predatory mite|XP 018495285.2 Fungia spp.|Coral|ffun1.m4.21870.m1
Araneus ventricosus|Orb-weaver spider|GBN06084
Tropilaelaps mercedesae|Honey bee mite|OQR67438
Hofstenia miamia|Three-banded panther worm|scaffold391 331241 359300 R
Praesagittifera naikaiensis|Acoel flatworm|g40739.t1 Hydra vulgaris|Fresh-water polyp|XP 012554388
Hydra vulgaris|Fresh-water polyp|XP 012561335 Hydra vulgaris|Fresh-water polyp|XP 012562662
Pocillopora damicornis|Lace coral| pdam 00020220-RA
Fungia spp.|Coral|ffun1.m4.18290.m1 Goniastrea aspera|Stony coral|gasp1.m3.6037.m1
Porites lutea|Small polyp stony coral|plut2.m8.21682.m1 Nematostella vectensis|Starlet sea anemone|Nemve1|102167
Aiptasia|Sea anemone|AIPGENE19094 Nematostella vectensis|Starlet sea anemone|Nemve1|85952
Trichonephila clavipes|Golden silk orb-weaver spider|PRD37692 Aiptasia|Sea anemone|AIPGENE12924 Ixodes scapularis|Black-legged tick|XP 029822890 Goniastrea aspera|Stony coral|gasp1.m3.26670.m1 Pocillopora damicornis|Lace coral|pdam 00014050-RA
Fungia spp.|Coral|ffun1.m4.18855.m1 Ixodes scapularis|Black-legged tick|XP 029845457 Praesagittifera naikaiensis|Acoel flatworm|g25700.t1
Parasteatoda tepidariorum|American house spider|XP 021001386 Branchiostoma belcheri|Lancelet|XP 019640450 Ixodes scapularis|Black-legged tick|XP 029843579 Homo sapiens|Human|TRPM1 CCDS10024.2 Homo sapiens|Human|TRPM3 CCDS6634.1 Centruroides sculpturatus|Arizona bark scorpion|XP 023240584 Homo sapiens|Human|TRPM6 CCDS6647.1 Homo sapiens|Human|TRPM7 CCDS42035.1
Araneus ventricosus|Orb-weaver spider|GBM00974 Limulus polyphemus|American horseshoe crab|XP 022248148
Ixodes scapularis|Black-legged tick|XP 029824440
Centruroides sculpturatus|Arizona bark scorpion|XP 023237252
Drosophila melanogaster|Common fruit fly|Trpm
Caenorhabditis elegans|Nematode|Cegtl-2 CE40563
Caenorhabditis elegans|Nematode|Cegon-2 CE30390 Leptotrombidium deliense|Chigger mite|RWS26931 Dinothrombium tinctorium|Giant velvet mite|RWS12646 Caenorhabditis elegans|Nematode|Cegtl-1 CE33754
Parasteatoda tepidariorum|American house spider|XP 015924394 Priapulus caudatus|Penis worm|XP 014671784 Aplysia californica|California sea hare|XP 012936450.1 Araneus ventricosus|Orb-weaver spider|GBM32255 Octopus vulgarisXP 029641654.1
Araneus ventricosus|Orb-weaver spider|GBM23289 Mizuhopecten yessoensis|Yesso scallop|OWF43174.1 Mizuhopecten yessoensis|Yesso scallop|XP 021368426.1
Dinothrombium tinctorium|Giant velvet mite|RWS01510 Mizuhopecten yessoensis|Yesso scallop|XP 021372912.1 Crassostrea gigas|Pacific Oyster|XP 011414379.1
Caenorhabditis elegans|Nematode|CED-11 CE00409 Crassostrea virginica|Eastern oyster|XP 022295841.1 Crassostrea gigas|Pacific Oyster|XP 011435194.1
Crassostrea gigas|Pacific Oyster|XP 011435204.1
Hydra vulgaris|Fresh-water polyp|XP 012565257
Ramazzottius varieornatus|Tardigrade|GAU92779 Aurelia|Moon jelly|Seg1831.8 transcript id Seg1831.8|GoldUCD|mRNA.D3Y31 Hypsibius dujardini|Tardigrade|OQV16369 Nematostella vectensis|Starlet sea anemone|Nemve1|248535 Ramazzottius varieornatus|Tardigrade|GAV05896 Crassostrea virginica|Eastern oyster|XP 022313978.1 Stylophora pistillata|Hood coral|Spis4675 SpisGene4675
Crassostrea virginica|Eastern oyster|XP 022305861.1 Pocillopora damicornis|Lace coral| pdam 00009856-RA Goniastrea aspera|Stony coral|gasp1.m3.30302.m1
Fungia spp.|Coral|ffun1.m4.19256.m1 Crassostrea gigas|Pacific Oyster|XP 019921765.1 Crassostrea virginica|Eastern oyster|XP 022342149.1 Porites lutea|Small polyp stony coral|plut2.m8.2561.m1 Mizuhopecten yessoensis|Yesso scallop|XP 021360214.1 Galaxea fascicularis|Galaxy coral|gfas1.m1.6599.m1 Pomacea canaliculata|Golden apple snail|XP 025088552.1 Acropora tenuis|Coral|aten 0.1.m1.1851.m1
Pomacea canaliculata|Golden apple snail|XP 025086702.1 Acropora digitifera|Coral|adi v1.10270
Branchiostoma belcheri|Lancelet|XP 019640471
Aplysia californica|California sea hare|XP 012937083.1 Branchiostoma belcheri|Lancelet|XP 019638014
Aplysia californica|California sea hare|XP 005091977.2 Aplysia californica|California sea hare|XP 012937912.1 Pomacea canaliculata|Golden apple snail|PVD39019.1
Crassostrea virginica|Eastern oyster|XP 022323164.1 Pomacea canaliculata|Golden apple snail|XP 025089091.1
Mizuhopecten yessoensis|Yesso scallop|OWF37798.1 Crassostrea gigas|Pacific Oyster|XP 019925619.1 Mizuhopecten yessoensis|Yesso scallop|OWF47104.1 Crassostrea virginica|Eastern oyster|XP 022319512.1 Crassostrea gigas|Pacific Oyster| XP 011414360.1 Octopus vulgaris|Common octopus|XP 029638531.1 Crassostrea gigas|Pacific Oyster|XP 011456090.1
Praesagittifera naikaiensis|Acoel flatworm|g14495.t1 Priapulus caudatus|Penis worm|XP 014670278 Praesagittifera naikaiensis|Acoel flatworm|g10675.t1 Branchiostoma belcheri|Lancelet|XP 019643532 Praesagittifera naikaiensis|Acoel flatworm|g14113.t1
Priapulus caudatus|Penis worm|XP 014669422
Homo sapiens|Human|TRPM2 CCDS13710.1
Homo sapiens|Human|TRPM8 CCDS33407.1
Homo sapiens|Human|TRPM4 CCDS33073.1
Homo sapiens|Human|TRPM5 CCDS31340.1
Octopus vulgaris|Common octopus|XP 029647149.1
Octopus vulgaris|Common octopus|XP 029646813.1
Octopus vulgaris|Common octopus|XP 029646968.1
Pomacea canaliculata|Golden apple snail|PVD35972.1
Pomacea canaliculata|Golden apple snail|XP 025081224.1
Aplysia californica|California sea hare|XP 012942949.1
Aplysia californica|California sea hare|XP 005100068.2
Aplysia californica|California sea hare|XP 012940727.1
Varroa jacobsoni|Asian honey bee mite|XP 022708485 Mizuhopecten yessoensis|Yesso scallop|XP 021375666.1
Mizuhopecten yessoensis|Yesso scallop|XP 021375672.1 Varroa destructor|Honey bee mite|XP 022649945 Crassostrea virginica|Eastern oyster|XP 022338390.1 Tropilaelaps mercedesae|Honey bee mite|OQR79469 Galendromus occidentalis|Western predatory mite|XP 028968955 Crassostrea virginica|Eastern oyster|XP 022339354.1
Crassostrea gigas|Pacific Oyster|XP 011446980.2
Ixodes scapularis|Black-legged tick|EEC04095 Crassostrea virginica|Eastern oyster|XP 022342892.1
Ixodes scapularis|Black-legged tick|XP 029828138 Mizuhopecten yessoensis|Yesso scallop|XP 021362498.1
Ixodes scapularis|Black-legged tick|XP 029831199 Crassostrea virginica|Eastern oyster|XP 022336960.1 Centruroides sculpturatus|Arizona bark scorpion|XP 023238125 Crassostrea gigas|Pacific Oyster|XP 019924531.1
Crassostrea gigas|Pacific Oyster|XP 011433270.1
Tetranychus urticae XP 015791381 Crassostrea gigas|Pacific Oyster|XP 019924535.1 Dinothrombium tinctorium|Giant velvet mite|RWS07648 Hydra vulgaris|Fresh-water polyp|XP 004208115.1 Parasteatoda tepidariorum|American house spider|XP 021001449 99 Aiptasia|Sea anemone|AIPGENE2913 Centruroides sculpturatus|Arizona bark scorpion|XP 023217962 Pocillopora damicornis|Lace coral|pdam 00008954-RA Centruroides sculpturatus|Arizona bark scorpion|XP 023217963 Stylophora pistillata|Hood coral|Spis7368
Limulus polyphemus|American horseshoe crab|XP 022246931 Fungia spp.|Coral|ffun1.m4.6390.m1 Limulus polyphemus|American horseshoe crab|XP 013776860 100 Porites lutea|Small polyp stony coral|plut2.m8.7778.m1 100 Galaxea fascicularis|Galaxy coral|gfas1.m1.15786.m1 Limulus polyphemus|American horseshoe crab|NP 001301012 Acropora tenuis|Coral|aten 0.1.m1.14307.m1 Parasteatoda tepidariorum|American house spider|XP 015916803 Acropora digitifera|Coral|adi v1.01512 Limulus polyphemus|American horseshoe crab|XP 013791151.2 Aiptasia|Sea anemone|AIPGENE5043
Drosophila melanogaster|Common fruit fly|Trpgamma Aiptasia|Sea anemone|AIPGENE2977 sp|P16157|ANK1 HUMAN Ankyrin-1 Dinothrombium tinctorium|Giant velvet mite|RWS15299 100 Fungia spp.|Coral|ffun1.m4.32623.m1 Porites lutea|Small polyp stony coral|plut2.m8.7792.m1 Leptotrombidium deliense|Chigger mite|RWS29242 Acropora digitifera|Coral|adi v1.17375 Tetranychus urticae XP 015788743 Acropora tenuis|Coral|aten 0.1.m1.14330.m1 Parasteatoda tepidariorum|American house spider|XP 015930751 Amplexidiscus fenestrafer|Elephant ear coral|evm.model.scaffold 24.48
Limulus polyphemus|American horseshoe crab|AIA22826 Pocillopora damicornis|Lace coral|pdam 00012740-RA
Centruroides sculpturatus|Arizona bark scorpion|XP 023236287 Stylophora pistillata|Hood coral|Spis7395
Drosophila melanogaster|Common fruit fly|Trp Caenorhabditis elegans|Nematode|trp-4 Y71A12B.4.1
Hypsibius dujardini|Tardigrade|OWA53387 Centruroides sculpturatus|Arizona bark scorpion|XP 023230521 Ramazzottius varieornatus|Tardigrade|GAU98766 Limulus polyphemus|American horseshoe crab|XP 013773346 Ramazzottius varieornatus|Tardigrade|GAU98765 Ixodes scapularis|Black-legged tick|XP 029844206 Hypsibius dujardini|Tardigrade|OWA53386 Drosophila melanogaster|Common fruit fly|Trpl Hypsibius dujardini|Tardigrade|OQV12783
Ramazzottius varieornatus|Tardigrade|GAU98498 Hypsibius dujardini|Tardigrade|OWA53384
Hypsibius dujardini|Tardigrade|OWA53372 Ramazzottius varieornatus|Tardigrade|GAU98762
Priapulus caudatus|Penis worm|XP 014667454 Ramazzottius varieornatus|Tardigrade|GAU98761 Ramazzottius varieornatus|Tardigrade|GAU98760 Caenorhabditis elegans|Nematode|trp-2 R06B10.4b.1 Priapulus caudatus|Penis worm|XP 014664542 Aplysia californica|California sea hare|XP 012934963 100 Branchiostoma belcheri|Lancelet|XP 019632148
Pomacea canaliculata|Golden apple snail|PVD34632 Drosophila melanogaster|Common fruit fly|nompC
Mizuhopecten yessoensis|Yesso scallop|XP 021346254 Octopus vulgaris|Common octopus|XP 029639474
Crassostrea virginica|Eastern oyster|XP 022324156 Pomacea canaliculata|Golden apple snail|XP 025111061 Pomacea canaliculata|Golden apple snail|PVD23826 Octopus vulgaris|Common octopus|XP 029644410 Homo sapiens|Human|TRPC5 CCDS14561.1 Mizuhopecten yessoensis|Yesso scallop|XP 021357228
Homo sapiens|Human|TRPC4 CCDS9365.1 Mizuhopecten yessoensis|Yesso scallop|XP 021357229 Mizuhopecten yessoensis|Yesso scallop|XP 021357230 Branchiostoma belcheri|Lancelet|XP 019647381 Crassostrea gigas|Pacific Oyster|XP 011437355 Branchiostoma belcheri|Lancelet|XP 019643927 Crassostrea gigas|Pacific Oyster|XP 011437356
Homo sapiens|Human|TRPC1 CCDS58856.1 Crassostrea gigas|Pacific Oyster|XP 019925747
Crassostrea gigas|Pacific Oyster|XP 011437357
Dinothrombium tinctorium|Giant velvet mite|RWS14118
Leptotrombidium deliense|Chigger mite|RWS27838 Hofstenia miamia|Three-banded panther worm|scaffold248 658024 661024 R Praesagittifera naikaiensis|Acoel flatworm|g15886.t1 Limulus polyphemus|American horseshoe crab|XP 022241744 Hofstenia miamia|Three-banded panther worm|scaffold266 245803 249552 R Praesagittifera naikaiensis|Acoel flatworm|g4329.t1 Araneus ventricosus|Orb-weaver spider|GBL94897
Praesagittifera naikaiensis|Acoel flatworm|g26659.t1 Parasteatoda tepidariorum|American house spider|XP 015918920 Ixodes scapularis|Black-legged tick|EEC03061 Tropilaelaps mercedesae|Honey bee mite|OQR78380
Galendromus occidentalis|Western predatory mite|XP 028968326 Ixodes scapularis|Black-legged tick|XP 029844767 Ixodes scapularis|Black-legged tick|EEC20553
Ixodes scapularis|Black-legged tick|XP 029828299
Parasteatoda tepidariorum|American house spider|XP 021002173 Ixodes scapularis|Black-legged tick|XP 029826751 Centruroides sculpturatus|Arizona bark scorpion|XP 023213474 Centruroides sculpturatus|Arizona bark scorpion|XP 023225280 Centruroides sculpturatus|Arizona bark scorpion|XP 023222540 Limulus polyphemus|American horseshoe crab|XP 013775532.2 Parasteatoda tepidariorum|American house spider|XP 015929354 Limulus polyphemus|American horseshoe crab|XP 022242800 Araneus ventricosus|Orb-weaver spider|GBM22717
Limulus polyphemus|American horseshoe crab|XP 013791075.2 Trichonephila clavipes|Golden silk orb-weaver spider|PRD32515 Branchiostoma belcheri|Lancelet|XP 019619618 Limulus polyphemus|American horseshoe crab|NP 001301020 Limulus polyphemus|American horseshoe crab|AAN38979 Praesagittifera naikaiensis|Acoel flatworm|g3329.t1
Praesagittifera naikaiensis|Acoel flatworm|g28454.t1 Araneus ventricosus|Orb-weaver spider|GBM30498 Praesagittifera naikaiensis|Acoel flatworm|g33793.t1 Parasteatoda tepidariorum|American house spider|XP 015904863 Branchiostoma belcheri|Lancelet|XP 019633316
Caenorhabditis elegans|Nematode|trp-1 ZC21.2b.1 Branchiostoma belcheri|Lancelet|XP 019622144 Centruroides sculpturatus|Arizona bark scorpion|XP 023238515 Branchiostoma belcheri|Lancelet|XP 019633932 Crassostrea virginica|Eastern oyster|XP 022321713 Crassostrea gigas|Pacific Oyster|XP 019924985 Branchiostoma belcheri|Lancelet|XP 019633683 Branchiostoma belcheri|Lancelet|XP 019621348
Branchiostoma belcheri|Lancelet|XP 019627222 Mizuhopecten yessoensis|Yesso scallop|XP 021376838 Octopus vulgaris|Common octopus|XP 029648709 Hypsibius dujardini|Tardigrade|OQV25244 Ramazzottius varieornatus|Tardigrade|GAV03645 Octopus vulgaris|Common octopus|XP 029648710 Dinothrombium tinctorium|Giant velvet mite|RWS01426
Limulus polyphemus|American horseshoe crab|XP 013790468 Branchiostoma belcheri|Lancelet|XP 019647217 Pomacea canaliculata|Golden apple snail|XP 025088295 Araneus ventricosus|Orb-weaver spider|GBM27668 Branchiostoma belcheri|Lancelet|XP 019647218 Parasteatoda tepidariorum|American house spider|XP 020999857
Centruroides sculpturatus|Arizona bark scorpion|XP 023241765 Crassostrea gigas|Pacific Oyster|XP 011428523 Centruroides sculpturatus|Arizona bark scorpion|XP 023209841
Centruroides sculpturatus|Arizona bark scorpion|XP 023235285 Crassostrea virginica|Eastern oyster|XP 022337866 Centruroides sculpturatus|Arizona bark scorpion|XP 023240102
Centruroides sculpturatus|Arizona bark scorpion|XP 023240103
Mizuhopecten yessoensis|Yesso scallop|XP 021357994 Centruroides sculpturatus|Arizona bark scorpion|XP 023218459
Pomacea canaliculata|Golden apple snail|PVD35107 Araneus ventricosus|Orb-weaver spider|GBL75431
Araneus ventricosus|Orb-weaver spider|GBL75433
Parasteatoda tepidariorum|American house spider|XP 015927173 Pomacea canaliculata|Golden apple snail|XP 025087043 Aplysia californica|California sea hare|XP 012936952 Limulus polyphemus|American horseshoe crab|XP 013791171.2
Limulus polyphemus|American horseshoe crab|XP 013778879 Pomacea canaliculata|Golden apple snail|PVD35106 Ixodes scapularis|Black-legged tick|XP 029826248 Aplysia californica|CaliforniaCrassostrea gigas|Pacific sea hare|XP Oyster|XP 012941918 011455889 Galendromus occidentalis|Western predatory mite|XP 018497405
Tropilaelaps mercedesae|Honey bee mite|OQR68673
Varroa jacobsoni|Asian honey bee mite|XP 022699354
Crassostrea virginica|Eastern oyster|XP 022328099 Varroa destructor|Honey bee mite|XP 022656457
Varroa destructor|Honey bee mite|XP 022656459
Varroa jacobsoni|Asian honey bee mite|XP 022699356
Aiptasia|Sea anemone|AIPGENE23973 Crassostrea virginica|Eastern oyster|XP 022319232 Pomacea canaliculata|Golden apple snail|XP 025087244 Nematostella vectensis|Starlet sea anemone|jgi Nemve1 92648 e gw.30.215.1
Acropora tenuis|Coral|aten 0.1.m1.10100.m1 CrassostreaCrassostrea virginica|Eastern gigas|Pacific oyster|XP Oyster|XP 022319233 011456753 Galaxea fascicularis|Galaxy coral|gfas1.m1.2217.m1
Goniastrea aspera|Stony coral|gasp1.m3.11339.m1 Crassostrea gigas|Pacific Oyster|XP 011456754 Fungia spp.|Coral|ffun1.m4.11228.m1
Amplexidiscus fenestrafer|Elephant ear anemone|evm.model.scaffold 331.7
Stylophora pistillata|Hood coral|Spis10260
Pocillopora damicornis|Lace coral|pdam 00003294-RA
Fungia spp.|Coral|ffun1.m4.21365.m1 Aplysia californica|California sea hare|XP 012943366 Galaxea fascicularis|Galaxy coral|gfas1.m1.19792.m1 Galaxea fascicularis|Galaxy coral|gfas1.m1.11320.m1
Goniastrea aspera|Stony coral|gasp1.m3.10244.m1
Stylophora pistillata|Hood coral|Spis9133
Acropora tenuis|Coral|aten 0.1.m1.11169.m1
Acropora tenuis|Coral|aten 0.1.m1.11152.m1 MizuhopectenCrassostrea yessoensis|Yesso virginica|Eastern scallop|XP oyster|XP 021340254 022298027 Galaxea fascicularis|Galaxy coral|gfas1.m1.9778.m1 Galaxea fascicularis|Galaxy coral|gfas1.m1.9779.m1 Acropora digitifera|Coral|adi v1.11450
Galaxea fascicularis|Galaxy coral|gfas1.m1.15325.m1
Stylophora pistillata|Hood coral|Spis9118 Porites lutea|Small polyp stony coral|plut2.m8.30328.m1
Porites lutea|Small polyp stony coral|plut2.m8.31261.m1 Crassostrea gigas|Pacific Oyster|XP 011420089 Nematostella vectensis|Starlet sea anemone|jgi Nemve1 197088 fgenesh1 pg.scaffold 4000168 Crassostrea virginica|Eastern oyster|XP 022302544 Aiptasia|Sea anemone|AIPGENE8755
Octopus vulgaris|Common octopus|XP 029637771 Mizuhopecten yessoensis|Yesso scallop|XP 021364913 Homo sapiens|Human|TRPC7 CCDS47267.2
Homo sapiens|Human|TRPC3 CCDS47130.1 Homo sapiens|Human|TRPC6 CCDS8311.1 Pomacea canaliculata|Golden apple snail|XP 025080613
Pomacea canaliculata|Golden apple snail|XP 025085325
Branchiostoma belcheri|Lancelet|XP 019625365 Acropora digitifera|Coral|adi v1.09061
Branchiostoma belcheri|Lancelet|XP 019629549 Acropora digitifera|Coral|adi v1.03002
Acropora tenuis|Coral|aten 0.1.m1.11120.m1
Praesagittifera naikaiensis|Acoel flatworm|g6354.t1
Praesagittifera naikaiensis|Acoel flatworm|g36825.t4 Fungia spp.|Coral|ffun1.m4.31112.m1
Fungia spp.|Coral|ffun1.m4.29139.m1
Acropora digitifera|Coral|adi v1.23533
Fungia spp.|Coral|ffun1.m4.27540.m1
Fungia spp.|Coral|ffun1.m4.25767.m1
Stylophora pistillata|Hood coral|Spis19227
Porites lutea|Small polyp stony coral|plut2.m8.25751.m1
Goniastrea aspera|Stony coral|gasp1.m3.27950.m1
Goniastrea aspera|Stony coral|gasp1.m3.27949.m1
Galaxea fascicularis|Galaxy coral|gfas1.m1.16337.m1 Goniastrea aspera|Stony coral|gasp1.m3.24839.m1
Galaxea fascicularis|Galaxy coral|gfas1.m1.1188.m1
Porites lutea|Small polyp stony coral|plut2.m8.33025.m1
Amplexidiscus fenestrafer|Elephant ear anemone|evm.model.scaffold 139.18 Amplexidiscus fenestrafer|Elephant ear anemone|evm.model.scaffold 66.47
Amplexidiscus fenestrafer|Elephant ear anemone|evm.model.scaffold 66.48
Nematostella vectensis|Starlet sea anemone|jgi Nemve1 31537 gw.8.57.1 Amplexidiscus fenestrafer|Elephant ear anemone|evm.model.scaffold 139.19
Amplexidiscus fenestrafer|Elephant ear anemone|evm.model.scaffold 66.49 UFboot Support
27 100
Figure S1 Tree scale: 1
TRP Family TRPS (soromelastatin)
TRPM (melastatin) TRPN (no mechanoreceptor potential C)
Parasteatoda tepidariorum|American house spider|XP 015907052
Limulus polyphemus|American horseshoe crab|XP 022255565
Parasteatoda tepidariorum|American house spider|XP 015912591
Pomacea canaliculata|Golden apple snail|PVD30001.1 Aplysia californica|California sea hare|XP 005094347.1
Centruroides sculpturatus|Arizona bark scorpion|XP 023241337 Dinothrombium tinctorium|Giant velvet mite|RWS01510 Araneus ventricosus|Orb-weaver spider|GBM10676 Trichonephila clavipes|Golden silk orb-weaver spider|PRD37692 TRPC (canonical) Araneus ventricosus|Orb-weaver spider|GBM32255 Araneus ventricosus|Orb-weaver spider|GBM23289 Dinothrombium tinctorium|Giant velvet mite|RWS12646 Parasteatoda tepidariorum|American house spider|XP 021001386 Leptotrombidium deliense|Chigger mite|RWS26931
Mizuhopecten yessoensisXP 021352500.1 Centruroides sculpturatus|Arizona bark scorpion|XP 023240584 Araneus ventricosus|Orb-weaver spider|GBN06084
Limulus polyphemus|American horseshoe crab|XP 022254350
Limulus polyphemus|American horseshoe crab|XP 022247894Araneus ventricosus|Orb-weaver spider|GBM00974
Limulus polyphemus|American horseshoe crab|XP 022250999
Galendromus occidentalis|Western predatory mite|XP 018495285.2 Ixodes scapularis|Black-legged tick|XP 029845457
Parasteatoda tepidariorum|American house spider|XPIxodes 015924394 scapularis|Black-legged tick|XP 029843579
TropilaelapsIxodes mercedesae|Honey scapularis|Black-legged bee mite|OQR67438 tick|XP 029822890
Mizuhopecten yessoensis|Yesso scallop|XP 021378397.1Mizuhopecten yessoensis|Yesso Crassostrea gigas|Pacific Oyster|XP 019919132.1 Crassostrea virginica|Eastern oyster|XP 022340640.1
Homo sapiens|Human|TRPM1 CCDS10024.2 Homo sapiens|Human|TRPM3 CCDS6634.1
Homo sapiens|Human|TRPM6 CCDS6647.1
Homo sapiens|Human|TRPM7 CCDS42035.1 Branchiostoma belcheri|Lancelet|XP 019640450 Ramazzottius varieornatus|Tardigrade|GAU92779 Caenorhabditis elegans|Nematode|Cegtl-2 CE40563 Caenorhabditis elegans|Nematode|Cegon-2 CE30390
Caenorhabditis elegans|Nematode|Cegtl-1 CE33754
Hypsibius dujardini|Tardigrade|OQV16369 Drosophila melanogaster|Common fruit fly|Trpm Ramazzottius varieornatus|Tardigrade|GAV05896 Limulus polyphemus|American horseshoe crab|XP 022248148 Caenorhabditis elegans|Nematode|CED-11 CE00409 Ixodes scapularis|Black-legged tick|XP 029824440 Centruroides sculpturatus|Arizona bark scorpion|XP 023237252
Priapulus caudatus|Penis worm|XP 014671784
Octopus vulgarisXP 029641654.1 Crassostrea virginica|Eastern oyster|XP 022313978.1 Aplysia californica|California sea hare|XP 012936450.1 Priapulus caudatus|Penis worm|XP 014670278 Mizuhopecten yessoensis|Yesso scallop|OWF43174.1 Crassostrea virginica|Eastern oyster|XP 022305861.1 Mizuhopecten yessoensis|Yesso scallop|XP 021368426.1 Mizuhopecten yessoensis|Yesso scallop|XP 021372912.1
Crassostrea gigas|Pacific Oyster|XP 011414379.1
Crassostrea virginica|Eastern oyster|XP 022295841.1 Crassostrea gigas|Pacific Oyster|XP 019921765.1 Crassostrea virginica|Eastern oyster|XP 022342149.1 Crassostrea gigas|Pacific Oyster|XP 011435194.1 Mizuhopecten yessoensis|Yesso scallop|XP 021360214.1 Crassostrea gigas|Pacific Oyster|XP 011435204.1
Pomacea canaliculata|Golden apple snail|XP 025088552.1 Branchiostoma belcheri|Lancelet|XP 019640471 Branchiostoma belcheri|Lancelet|XP 019638014 Pomacea canaliculata|Golden apple snail|XP 025086702.1 Aplysia californica|California sea hare|XP 005091977.2 Pomacea canaliculata|Golden apple snail|PVD39019.1
Pomacea canaliculata|Golden apple snail|XP 025089091.1 Aplysia californica|California sea hare|XP 012937083.1 Mizuhopecten yessoensis|Yesso scallop|OWF37798.1 Aplysia californica|California sea hare|XP 012937912.1 Crassostrea virginica|Eastern oyster|XP 022319512.1 Crassostrea gigas|Pacific Oyster| XP 011414360.1
Crassostrea virginica|Eastern oyster|XP 022323164.1 Crassostrea gigas|Pacific Oyster|XP 011456090.1
Homo sapiens|Human|TRPM2 CCDS13710.1
Crassostrea gigas|Pacific Oyster|XP 019925619.1 Homo sapiens|Human|TRPM8 CCDS33407.1 Mizuhopecten yessoensis|Yesso scallop|OWF47104.1 Homo sapiens|Human|TRPM4 CCDS33073.1
Homo sapiens|Human|TRPM5 CCDS31340.1 Octopus vulgaris|Common octopus|XP 029638531.1 Priapulus caudatus|Penis worm|XP 014669422
Octopus vulgaris|Common octopus|XP 029647149.1 Branchiostoma belcheri|Lancelet|XP 019643532 Octopus vulgaris|Common octopus|XP 029646813.1
Octopus vulgaris|Common octopus|XP 029646968.1
Pomacea canaliculata|Golden apple snail|PVD35972.1
Pomacea canaliculata|Golden apple snail|XP 025081224.1
Aplysia californica|California sea hare|XP 012942949.1
Aplysia californica|California sea hare|XP 005100068.2
Aplysia californica|California sea hare|XP 012940727.1
Mizuhopecten yessoensis|Yesso scallop|XP 021362498.1
Crassostrea virginica|Eastern oyster|XP 022336960.1
Crassostrea gigas|Pacific Oyster|XP 019924531.1
Varroa jacobsoni|Asian honey bee mite|XP 022708485 Crassostrea gigas|Pacific Oyster|XP 011433270.1 Crassostrea gigas|Pacific Oyster|XP 019924535.1
Galendromus occidentalis|WesternVarroa destructor|Honey predatory bee mite|XP mite|XP 028968955 022649945 Mizuhopecten yessoensis|Yesso scallop|XP 021375666.1 Mizuhopecten yessoensis|Yesso scallop|XP 021375672.1
Tropilaelaps mercedesae|Honey bee mite|OQR79469 Crassostrea gigas|Pacific Oyster|XP 011446980.2 Crassostrea virginica|Eastern oyster|XP 022342892.1
Ixodes scapularis|Black-legged tick|EEC04095 Crassostrea virginica|Eastern oyster|XP 022338390.1 Ixodes scapularis|Black-legged tick|XP 029828138 93 Crassostrea virginica|Eastern oyster|XP 022339354.1
Ixodes scapularis|Black-legged tick|XP 029831199 Ramazzottius varieornatus|Tardigrade|GAU98765 Centruroides sculpturatus|Arizona bark scorpion|XP 023238125 Hypsibius dujardini|Tardigrade|OWA53386 Limulus polyphemus|American horseshoe crab|XP 022246931 100 Hypsibius dujardini|Tardigrade|OWA53387 Ramazzottius varieornatus|Tardigrade|GAU98766 Limulus polyphemus|American horseshoe crab|XP 013776860 100 Hypsibius dujardini|Tardigrade|OQV12783
Limulus polyphemus|American horseshoe crab|NP 001301012 Hypsibius dujardini|Tardigrade|OWA53384
Parasteatoda tepidariorum|American house spider|XP 015916803 Ramazzottius varieornatus|Tardigrade|GAU98761 Ramazzottius varieornatus|Tardigrade|GAU98762 Limulus polyphemus|American horseshoe crab|XP 013791151.2 100 Ramazzottius varieornatus|Tardigrade|GAU98760 Centruroides sculpturatus|Arizona bark scorpion|XP 023217962 Caenorhabditis elegans|Nematode|trp-4 Y71A12B.4.1
Centruroides sculpturatus|Arizona bark scorpion|XP 023217963 Priapulus caudatus|Penis worm|XP 014664542 Branchiostoma belcheri|Lancelet|XP 019632148 Parasteatoda tepidariorum|American house spider|XP 021001449 Octopus vulgaris|Common octopus|XP 029639474 Tetranychus urticae XP 015791381 Pomacea canaliculata|Golden apple snail|XP 025111061
Dinothrombium tinctorium|Giant velvet mite|RWS07648 Pomacea canaliculata|Golden apple snail|PVD23826
Drosophila melanogaster|Common fruit fly|Trpgamma Mizuhopecten yessoensis|Yesso scallop|XP 021357229 Mizuhopecten yessoensis|Yesso scallop|XP 021357228 Dinothrombium tinctorium|Giant velvet mite|RWS15299 Mizuhopecten yessoensis|Yesso scallop|XP 021357230 Leptotrombidium deliense|Chigger mite|RWS29242 Crassostrea gigas|Pacific Oyster|XP 011437357 Tetranychus urticae XP 015788743 Crassostrea gigas|Pacific Oyster|XP 011437355
Crassostrea gigas|Pacific Oyster|XP 019925747 Parasteatoda tepidariorum|American house spider|XP 015930751 100 Crassostrea gigas|Pacific Oyster|XP 011437356 Limulus polyphemus|American horseshoe crab|AIA22826 Drosophila melanogaster|Common fruit fly|nompC
Dinothrombium tinctorium|Giant velvet mite|RWS14118 Centruroides sculpturatus|Arizona bark scorpion|XP 023236287 Drosophila melanogaster|Common fruit fly|Trp Leptotrombidium deliense|Chigger mite|RWS27838
Limulus polyphemus|American horseshoe crab|XP 022241744
Centruroides sculpturatus|Arizona bark scorpion|XP 023230521 Centruroides sculpturatus|Arizona bark scorpion|XP 023213474
Limulus polyphemus|American horseshoe crab|XP 013773346 Centruroides sculpturatus|Arizona bark scorpion|XP 023222540 Ixodes scapularis|Black-legged tick|XP 029844206 Parasteatoda tepidariorum|American house spider|XP 015929354 Drosophila melanogaster|Common fruit fly|Trpl Araneus ventricosus|Orb-weaver spider|GBM22717
Ramazzottius varieornatus|Tardigrade|GAU98498 Trichonephila clavipes|Golden silk orb-weaver spider|PRD32515 Hypsibius dujardini|Tardigrade|OWA53372 Araneus ventricosus|Orb-weaver spider|GBL94897
Parasteatoda tepidariorum|American house spider|XP 015918920
Caenorhabditis elegans|Nematode|trp-2 R06B10.4b.1 Tropilaelaps mercedesae|Honey bee mite|OQR78380 Galendromus occidentalis|Western predatory mite|XP 028968326 Aplysia californica|California sea hare|XP 012934963 Ixodes scapularis|Black-legged tick|EEC20553
Pomacea canaliculata|Golden apple snail|PVD34632 Ixodes scapularis|Black-legged tick|XP 029828299
Ixodes scapularis|Black-legged tick|XP 029826751 Mizuhopecten yessoensis|Yesso scallop|XP 021346254 Branchiostoma belcheri|Lancelet|XP 019619618 Crassostrea virginica|Eastern oyster|XP 022324156 Branchiostoma belcheri|Lancelet|XP 019627222 Octopus vulgaris|Common octopus|XP 029644410 Branchiostoma belcheri|Lancelet|XP 019633316 Priapulus caudatus|Penis worm|XP 014667454 Branchiostoma belcheri|Lancelet|XP 019622144 Homo sapiens|Human|TRPC5 CCDS14561.1 Branchiostoma belcheri|Lancelet|XP 019633932
Homo sapiens|Human|TRPC4 CCDS9365.1 Branchiostoma belcheri|Lancelet|XP 019633683
Branchiostoma belcheri|Lancelet|XP 019621348
Branchiostoma belcheri|Lancelet|XP 019647381 Hypsibius dujardini|Tardigrade|OQV25244
Ramazzottius varieornatus|Tardigrade|GAV03645 Branchiostoma belcheri|Lancelet|XP 019643927 Homo sapiens|Human|TRPC1 CCDS58856.1 Dinothrombium tinctorium|Giant velvet mite|RWS01426 Limulus polyphemus|American horseshoe crab|XP 013790468
Araneus ventricosus|Orb-weaver spider|GBM27668
Parasteatoda tepidariorum|American house spider|XP 020999857
Centruroides sculpturatus|Arizona bark scorpion|XP 023241765
Centruroides sculpturatus|Arizona bark scorpion|XP 023209841
Limulus polyphemus|American horseshoe crab|XP 013775532.2 Centruroides sculpturatus|Arizona bark scorpion|XP 023235285 Limulus polyphemus|American horseshoe crab|XP 022242800 Centruroides sculpturatus|Arizona bark scorpion|XP 023240102 Centruroides sculpturatus|Arizona bark scorpion|XP 023240103
Limulus polyphemus|American horseshoe crab|XP 013791171.2
Limulus polyphemus|American horseshoe crab|XP 013791075.2 Limulus polyphemus|American horseshoe crab|XP 013778879 Limulus polyphemus|American horseshoe crab|NP 001301020 Centruroides sculpturatus|Arizona bark scorpion|XP 023218459 Limulus polyphemus|AmericanIxodes scapularis|Black-legged horseshoe crab|AAN38979 tick|EEC03061 Araneus ventricosus|Orb-weaver spider|GBL75431 Araneus ventricosus|Orb-weaver spider|GBL75433
Parasteatoda tepidariorum|American house spider|XP 015927173
Ixodes scapularis|Black-legged tick|XP 029826248 Ixodes scapularis|Black-legged tick|XP 029844767 Centruroides sculpturatus|Arizona bark scorpion|XP 023225280 Galendromus occidentalis|Western predatory mite|XP 018497405 Tropilaelaps mercedesae|Honey bee mite|OQR68673
Varroa destructor|Honey bee mite|XP 022656457
Varroa jacobsoni|Asian honey bee mite|XP 022699354
Varroa destructor|Honey bee mite|XP 022656459
Varroa jacobsoni|Asian honey bee mite|XP 022699356
Pomacea canaliculata|Golden apple snail|XP 025085325
Octopus vulgaris|Common octopus|XP 029637771
Pomacea canaliculata|Golden apple snail|XP 025080613 Araneus ventricosus|Orb-weaver spider|GBM30498 Mizuhopecten yessoensis|Yesso scallop|XP 021364913 Crassostrea gigas|Pacific Oyster|XP 011420089
Crassostrea virginica|Eastern oyster|XP 022302544
Crassostrea virginica|Eastern oyster|XP 022298027
Branchiostoma belcheri|Lancelet|XP 019629549
Branchiostoma belcheri|Lancelet|XP 019625365
Homo sapiens|Human|TRPC3 CCDS47130.1 Homo sapiens|Human|TRPC6 CCDS8311.1 Parasteatoda tepidariorum|American house spider|XP 021002173
Parasteatoda tepidariorum|AmericanCrassostrea house spider|XPCrassostrea virginica|Eastern 015904863 gigas|Pacific oyster|XP Oyster|XP 022321713 019924985
Centruroides sculpturatus|Arizona bark scorpion|XP 023238515
Mizuhopecten yessoensis|Yesso scallop|XP 021376838 Octopus vulgaris|Common octopus|XP 029648709
Pomacea canaliculata|GoldenOctopus apple vulgaris|Common snail|XP 025088295 octopus|XP 029648710 Branchiostoma belcheri|Lancelet|XP 019647217
Branchiostoma belcheri|Lancelet|XP 019647218
Caenorhabditis elegans|Nematode|trp-1Crassostrea gigas|Pacific ZC21.2b.1Oyster|XP 011428523
Crassostrea virginica|Eastern oyster|XP 022337866
Pomacea canaliculata|Golden apple snail|PVD35107 Mizuhopecten yessoensis|Yesso scallop|XP 021357994
Aplysia californica|California sea hare|XP 012936952 Crassostrea gigas|Pacific Oyster|XP 011455889
Pomacea canaliculata|Golden apple snail|XPPomacea 025087043 canaliculata|Golden apple snail|PVD35106
Aplysia californica|California sea hare|XP 012941918 Homo sapiens|Human|TRPC7 CCDS47267.2
Crassostrea virginica|Eastern oyster|XP 022328099 Crassostrea gigas|Pacific Oyster|XP 011456753
Crassostrea gigas|Pacific Oyster|XP 011456754
Crassostrea virginica|Eastern oyster|XP 022319232 Crassostrea virginica|Eastern oyster|XP 022319233 UFboot Support
Pomacea canaliculata|Golden apple snail|XP 025087244 Aplysia californica|California sea hare|XP 012943366
Mizuhopecten yessoensis|Yesso scallop|XP 021340254 17 100
Figure S2 TRP Family TRPS (soromelastatin)
TRPM (melastatin) TRPN (no mechanoreceptor potential C)
Hofstenia miamia|Three-banded panther worm|scaffold387 254022 257456 F
Hofstenia miamia|Three-banded panther worm|scaffold387 247539 251000 F
Hofstenia miamia|Three-banded panther worm|scaffold387 196851 200279 F
Hofstenia miamia|Three-banded panther worm|scaffold387 211324 251000 F TRPC (canonical) Hofstenia miamia|Three-banded panther worm|scaffold391 331241 359300 R Hofstenia miamia|Three-banded panther worm|scaffold387 219858 223314 F
Caenorhabditis elegans|Nematode|Cegon-2 CE30390 Mizuhopecten yessoensis|Yesso scallop|XP 021368426.1
Mizuhopecten yessoensis|Yesso scallop|XP 021372912.1
Hofstenia miamia|Three-banded panther worm|scaffold488 5372 8923 F Praesagittifera naikaiensis|Acoel flatworm|g25700.t1 Caenorhabditis elegans|Nematode|Cegtl-1 CE33754
Caenorhabditis elegans|Nematode|Cegtl-2 CE40563
Mizuhopecten yessoensis|Yesso scallop|OWF43174.1
Crassostrea gigas|Pacific Oyster|XP 011435194.1
Crassostrea gigas|Pacific Oyster|XP 011435204.1
Aplysia californica|California sea hare|XP 012936450.1 Crassostrea virginica|Eastern oyster|XP 022295841.1 Hofstenia miamia|Three-banded panther worm|scaffold18 866412 869501 F Homo sapiens|Human|TRPM7 CCDS42035.1
Crassostrea gigas|Pacific Oyster|XP 011414379.1 Hofstenia miamia|Three-banded panther worm|scaffold18 807971 810976 F Hofstenia miamia|Three-banded panther worm|scaffold112 437721 469571 R
Praesagittifera naikaiensis|Acoel flatworm|g16021.t1 Octopus vulgarisXP 029641654.1
Praesagittifera naikaiensis|Acoel flatworm|g36760.t1
Centruroides sculpturatus|Arizona bark scorpion|XP 023240584 Praesagittifera naikaiensis|Acoel flatworm|g4935.t1 Praesagittifera naikaiensis|Acoel flatworm|g11490.t1 Centruroides sculpturatus|Arizona bark scorpion|XP 023241337
Limulus polyphemus|American horseshoe crab|XP 022250999
Limulus polyphemus|American horseshoe crab|XP 022254350
Limulus polyphemus|American horseshoe crab|XP 022247894
Parasteatoda tepidariorum|American house spider|XP 021001386
Trichonephila clavipes|Golden silk orb-weaver spider|PRD37692 Parasteatoda tepidariorum|American house spider|XP 015912591 Ixodes scapularis|Black-legged tick|XP 029822890
Homo sapiens|Human|TRPM6 CCDS6647.1
Branchiostoma belcheri|Lancelet|XP 019640450 Homo sapiens|Human|TRPM3 CCDS6634.1
Homo sapiens|Human|TRPM1 CCDS10024.2 Araneus ventricosus|Orb-weaver spider|GBM00974 Drosophila melanogaster|Common fruit fly|Trpm Priapulus caudatus|Penis worm|XP 014671784
Centruroides sculpturatus|Arizona bark scorpion|XP 023237252
Limulus polyphemus|American horseshoe crab|XP 022248148
Ixodes scapularis|Black-legged tick|XP 029824440
Fungia spp.|Coral|ffun1.m4.19256.m1
Acropora digitifera|Coral|adi v1.10270
Acropora tenuis|Coral|aten 0.1.m1.1851.m1 Porites lutea|Small polyp stony coral|plut2.m8.2561.m1
Galaxea fascicularis|Galaxy coral|gfas1.m1.6599.m1
Goniastrea aspera|Stony coral|gasp1.m3.30302.m1
Pocillopora damicornis|Lace coral| pdam 00009856-RA
Stylophora pistillata|Hood coral|Spis4675 SpisGene4675
Parasteatoda tepidariorum|American house spider|XP 015907052
DinothrombiumAraneus tinctorium|Giant ventricosus|Orb-weaver velvet mite|RWS12646 spider|GBN06084 Fungia spp.|Coral|ffun1.m4.21870.m1
Pocillopora damicornis|Lace coral| pdam 00007420-RA
Stylophora pistillata|Hood coral|Spis14035.t1 SpisGene14035
Goniastrea aspera|Stony coral|gasp1.m3.6245.m1 Parasteatoda tepidariorum|American house spider|XP 015924394 Leptotrombidium deliense|Chigger mite|RWS26931 Galaxea fascicularis|Galaxy coral|gfas1.m1.11287.m1
Galendromus occidentalis|Western predatory mite|XP 018495285.2 Mizuhopecten yessoensisXP 021352500.1 Ixodes scapularis|Black-legged tick|XP 029843579 Pomacea canaliculata|Golden apple snail|PVD30001.1 Aplysia californica|California sea hare|XP 005094347.1
Ixodes scapularis|Black-legged tick|XP 029845457 Mizuhopecten yessoensis|Yesso scallop|XP 021378397.1
Crassostrea virginica|Eastern oyster|XP 022340640.1
Crassostrea gigas|Pacific Oyster|XP 019919132.1
Hydra vulgaris|Fresh-water polyp|XP 012562662
Tropilaelaps mercedesae|Honey bee mite|OQR67438 Aurelia|Moon jelly|Seg1803.3 transcript id=Seg1803.3|GoldUCD|mRNA.D3Y31
Aiptasia|Sea anemone|AIPGENE12924 Araneus ventricosus|Orb-weaver spider|GBM23289 Aiptasia|Sea anemone|AIPGENE19094 Pocillopora damicornis|Lace coral|pdam 00014050-RA Araneus ventricosus|Orb-weaver spider|GBM32255 Dinothrombium tinctorium|Giant velvet mite|RWS01510 Goniastrea aspera|Stony coral|gasp1.m3.26670.m1 Fungia spp.|Coral|ffun1.m4.18855.m1
Crassostrea virginica|Eastern oyster|XP 022323164.1 Nematostella vectensis|Starlet sea anemone|Nemve1|85952 Nematostella vectensis|Starlet sea anemone|Nemve1|102167 Mizuhopecten yessoensis|Yesso scallop|OWF47104.1 Porites lutea|Small polyp stony coral|plut2.m8.21682.m1
Pocillopora damicornis|Lace coral| pdam 00020220-RA Octopus vulgaris|Common octopus|XP 029638531.1 Goniastrea aspera|Stony coral|gasp1.m3.6037.m1
Pomacea canaliculata|Golden apple snail|XP 025086702.1 Fungia spp.|Coral|ffun1.m4.18290.m1 Priapulus caudatus|Penis worm|XP 014670278 Pomacea canaliculata|Golden apple snail|XP 025088552.1 Crassostrea virginica|Eastern oyster|XP 022336960.1 Crassostrea gigas|Pacific Oyster|XP 019924531.1
Crassostrea gigas|Pacific Oyster|XP 019924535.1 Aplysia californica|California sea hare|XP 012937912.1 Crassostrea gigas|Pacific Oyster|XP 011433270.1 Aplysia californica|California sea hare|XP 012937083.1 Crassostrea virginica|Eastern oyster|XP 022339354.1
Crassostrea virginica|Eastern oyster|XP 022338390.1 Crassostrea virginica|Eastern oyster|XP 022305861.1 Branchiostoma belcheri|Lancelet|XP 019638014 Crassostrea virginica|Eastern oyster|XP 022313978.1 Crassostrea gigas|Pacific Oyster| XP 011414360.1
Pomacea canaliculata|Golden apple snail|XP 025089091.1 Crassostrea gigas|Pacific Oyster|XP 019921765.1 Crassostrea virginica|Eastern oyster|XP 022342149.1 Pomacea canaliculata|Golden apple snail|PVD39019.1 Mizuhopecten yessoensis|Yesso scallop|XP 021360214.1 Aplysia californica|California sea hare|XP 005091977.2
Mizuhopecten yessoensis|Yesso scallop|OWF37798.1
Crassostrea virginica|Eastern oyster|XP 022319512.1 Branchiostoma belcheri|Lancelet|XP 019643532 Crassostrea gigas|Pacific Oyster|XP 011456090.1 Ramazzottius varieornatus|Tardigrade|GAV05896 Praesagittifera naikaiensis|Acoel flatworm|g40739.t1
Crassostrea gigas|Pacific Oyster|XP 019925619.1 Priapulus caudatus|Penis worm|XP 014669422
Ramazzottius varieornatus|Tardigrade|GAU92779 Hydra vulgaris|Fresh-water polyp|XP 012561335 Hydra vulgaris|Fresh-water polyp|XP 012554388
Praesagittifera naikaiensis|Acoel flatworm|g14113.t1 CaenorhabditisHypsibius elegans|Nematode|CED-11 dujardini|Tardigrade|OQV16369 CE00409 Praesagittifera naikaiensis|Acoel flatworm|g10675.t1
Limulus polyphemus|American horseshoe crab|XP 022255565
Araneus ventricosus|Orb-weaver spider|GBM10676
Homo sapiens|Human|TRPM8 CCDS33407.1
Homo sapiens|Human|TRPM5 CCDS31340.1
Homo sapiens|Human|TRPM4 CCDS33073.1
Mizuhopecten yessoensis|Yesso scallop|XP 021375672.1
Praesagittifera naikaiensis|Acoel flatworm|g14495.t1
Aurelia|Moon jelly|Seg1831.8 transcript id=Seg1831.8|GoldUCD|mRNA.D3Y31
Hydra vulgaris|Fresh-water polyp|XP 012565257
Crassostrea virginica|Eastern oyster|XP 022342892.1
Crassostrea gigas|Pacific Oyster|XP 011446980.2
Branchiostoma belcheri|Lancelet|XP 019640471
Octopus vulgaris|Common octopus|XP 029647149.1
Crassostrea virginica|Eastern oyster|XP 022324156 Octopus vulgaris|Common octopus|XP 029646968.1
Mizuhopecten yessoensis|Yesso scallop|XP 021346254 Octopus vulgaris|Common octopus|XP 029646813.1
Aplysia californica|California sea hare|XP 012940727.1 Pomacea canaliculata|Golden apple snail|PVD34632 Aplysia californica|California sea hare|XP 005100068.2 Aplysia californica|California sea hare|XP 012934963 Homo sapiens|Human|TRPM2 CCDS13710.1
Octopus vulgaris|Common octopus|XP 029644410 Mizuhopecten yessoensis|Yesso scallop|XP 021375666.1
Caenorhabditis elegans|Nematode|trp-2 R06B10.4b.1 Nematostella vectensis|Starlet sea anemone|Nemve1|248535
Pomacea canaliculata|Golden apple snail|XP 025081224.1 Priapulus caudatus|Penis worm|XP 014667454 Pomacea canaliculata|Golden apple snail|PVD35972.1 Hypsibius dujardini|Tardigrade|OWA53372 Aplysia californica|California sea hare|XP 012942949.1 Ramazzottius varieornatus|Tardigrade|GAU98498 Mizuhopecten yessoensis|Yesso scallop|XP 021362498.1
Homo sapiens|Human|TRPC4 CCDS9365.1 Aiptasia|Sea anemone|AIPGENE5043
Homo sapiens|Human|TRPC5 CCDS14561.1 Stylophora pistillata|Hood coral|Spis7395
Branchiostoma belcheri|Lancelet|XP 019647381 Acropora digitifera|Coral|adi v1.17375
Fungia spp.|Coral|ffun1.m4.32623.m1 Homo sapiens|Human|TRPC1 CCDS58856.1 Pocillopora damicornis|Lace coral|pdam 00012740-RA Branchiostoma belcheri|Lancelet|XP 019643927 100 Acropora digitifera|Coral|adi v1.01512 Praesagittifera naikaiensis|Acoel flatworm|g4329.t1 Stylophora pistillata|Hood coral|Spis7368 Praesagittifera naikaiensis|Acoel flatworm|g15886.t1 100 Pocillopora damicornis|Lace coral|pdam 00008954-RA Hofstenia miamia|Three-banded panther worm|scaffold266 245803 249552 R Fungia spp.|Coral|ffun1.m4.6390.m1 Hofstenia miamia|Three-banded panther worm|scaffold248 658024 661024 R Porites lutea|Small polyp stony coral|plut2.m8.7778.m1
Praesagittifera naikaiensis|Acoel flatworm|g26659.t1 Acropora tenuis|Coral|aten 0.1.m1.14307.m1
Galaxea fascicularis|Galaxy coral|gfas1.m1.15786.m1 Homo sapiens|Human|TRPC3 CCDS47130.1 100 Ramazzottius varieornatus|Tardigrade|GAU98766 Homo sapiens|Human|TRPC7 CCDS47267.2 Ramazzottius varieornatus|Tardigrade|GAU98761 Homo sapiens|Human|TRPC6 CCDS8311.1 Ramazzottius varieornatus|Tardigrade|GAU98762 Branchiostoma belcheri|Lancelet|XP 019625365 Araneus ventricosus|Orb-weaver spider|GBM22717
Branchiostoma belcheri|Lancelet|XP 019629549 Centruroides sculpturatus|Arizona bark scorpion|XP 023213474
Pomacea canaliculata|Golden apple snail|XP 025085325 Tropilaelaps mercedesae|Honey bee mite|OQR78380
Branchiostoma belcheri|Lancelet|XP 019621348 Galendromus occidentalis|Western predatory mite|XP 028968326 Ixodes scapularis|Black-legged tick|XP 029826751 Pomacea canaliculata|Golden apple snail|XP 025080613 100 Ixodes scapularis|Black-legged tick|XP 029828299 Octopus vulgaris|Common octopus|XP 029637771 Ixodes scapularis|Black-legged tick|EEC20553 Branchiostoma belcheri|Lancelet|XP 019633932 Leptotrombidium deliense|Chigger mite|RWS27838 Branchiostoma belcheri|Lancelet|XP 019633683 Dinothrombium tinctorium|Giant velvet mite|RWS14118
Branchiostoma belcheri|Lancelet|XP 019622144 Parasteatoda tepidariorum|American house spider|XP 015929354 Branchiostoma belcheri|Lancelet|XP 019619618 100 Trichonephila clavipes|Golden silk orb-weaver spider|PRD32515 Branchiostoma belcheri|Lancelet|XP 019633316 Limulus polyphemus|American horseshoe crab|XP 022241744 Centruroides sculpturatus|Arizona bark scorpion|XP 023222540 Branchiostoma belcheri|Lancelet|XP 019627222 Parasteatoda tepidariorum|American house spider|XP 015918920 Praesagittifera naikaiensis|Acoel flatworm|g36825.t4 Araneus ventricosus|Orb-weaver spider|GBL94897 Praesagittifera naikaiensis|Acoel flatworm|g6354.t1 Ramazzottius varieornatus|Tardigrade|GAU98765
Praesagittifera naikaiensis|Acoel flatworm|g33793.t1 Hypsibius dujardini|Tardigrade|OWA53386
Praesagittifera naikaiensis|Acoel flatworm|g28454.t1 Hydra vulgaris|Fresh-water polyp|XP 004208115.1 Aiptasia|Sea anemone|AIPGENE2913
Centruroides sculpturatus|Arizona bark scorpion|XP 023240102 Aiptasia|Sea anemone|AIPGENE2977 sp|P16157|ANK1 HUMAN Ankyrin-1
Centruroides sculpturatus|Arizona bark scorpion|XP 023240103 Acropora tenuis|Coral|aten 0.1.m1.14330.m1 Amplexidiscus fenestrafer|Elephant ear coral|evm.model.scaffold 24.48 Centruroides sculpturatus|Arizona bark scorpion|XP 023235285 Porites lutea|Small polyp stony coral|plut2.m8.7792.m1 Centruroides sculpturatus|Arizona bark scorpion|XP 023241765 Hypsibius dujardini|Tardigrade|OWA53387 Centruroides sculpturatus|Arizona bark scorpion|XP 023209841 Araneus ventricosus|Orb-weaver spider|GBM27668 Hypsibius dujardini|Tardigrade|OQV12783 Ramazzottius varieornatus|Tardigrade|GAU98760
Hypsibius dujardini|Tardigrade|OWA53384 Parasteatoda tepidariorum|American house spider|XP 020999857 Dinothrombium tinctorium|Giant velvet mite|RWS01426 Caenorhabditis elegans|Nematode|trp-4 Y71A12B.4.1
Hypsibius dujardini|Tardigrade|OQV25244 Drosophila melanogaster|Common fruit fly|nompC Limulus polyphemus|American horseshoe crab|XP 013790468 Crassostrea gigas|Pacific Oyster|XP 011437357
Ramazzottius varieornatus|Tardigrade|GAV03645 Crassostrea gigas|Pacific Oyster|XP 019925747
Crassostrea gigas|Pacific Oyster|XP 011437355 Araneus ventricosus|Orb-weaver spider|GBL75431 Crassostrea gigas|Pacific Oyster|XP 011437356
Pomacea canaliculata|Golden apple snail|PVD23826 Ixodes scapularis|Black-legged tick|XP 029826248 Centruroides sculpturatus|Arizona bark scorpion|XP 023218459 Pomacea canaliculata|Golden apple snail|XP 025111061
Mizuhopecten yessoensis|Yesso scallop|XP 021357230
Mizuhopecten yessoensis|Yesso scallop|XP 021357228 Limulus polyphemus|American horseshoe crab|XP 013791171.2 Mizuhopecten yessoensis|Yesso scallop|XP 021357229 Limulus polyphemus|AmericanAraneus ventricosus|Orb-weaver horseshoe crab|XP spider|GBL75433 013778879 Octopus vulgaris|Common octopus|XP 029639474
Priapulus caudatus|Penis worm|XP 014664542
Praesagittifera naikaiensis|Acoel flatworm|g3329.t1 Branchiostoma belcheri|Lancelet|XP 019632148 Parasteatoda tepidariorum|American house spider|XP 015927173 Fungia spp.|Coral|ffun1.m4.21365.m1
Pomacea canaliculata|Golden apple snail|XP 025087043 Galaxea fascicularis|Galaxy coral|gfas1.m1.19792.m1 Pomacea canaliculata|Golden apple snail|PVD35107 Galaxea fascicularis|Galaxy coral|gfas1.m1.11320.m1
Aplysia californica|California sea hare|XP 012936952 Goniastrea aspera|Stony coral|gasp1.m3.10244.m1
Crassostrea virginica|Eastern oyster|XP 022337866 Stylophora pistillata|Hood coral|Spis9133 Crassostrea gigas|Pacific Oyster|XP 011428523 Acropora tenuis|Coral|aten 0.1.m1.11169.m1
Acropora tenuis|Coral|aten 0.1.m1.11152.m1
Mizuhopecten yessoensis|Yesso scallop|XP 021357994 Galaxea fascicularis|Galaxy coral|gfas1.m1.9778.m1 Pomacea canaliculata|Golden apple snail|PVD35106 Galaxea fascicularis|Galaxy coral|gfas1.m1.9779.m1
Porites lutea|Small polyp stony coral|plut2.m8.31261.m1 Aplysia californica|California sea hare|XP 012941918 Porites lutea|Small polyp stony coral|plut2.m8.30328.m1 Crassostrea virginica|Eastern oyster|XP 022328099 Crassostrea gigas|Pacific Oyster|XP 011455889 Stylophora pistillata|Hood coral|Spis9118
Acropora digitifera|Coral|adi v1.11450
Galaxea fascicularis|Galaxy coral|gfas1.m1.15325.m1
Pomacea canaliculata|Golden apple snail|XP 025087244 Fungia spp.|Coral|ffun1.m4.31112.m1 Crassostrea virginica|Eastern oyster|XP 022319233 Acropora digitifera|Coral|adi v1.23533
Crassostrea virginica|Eastern oyster|XP 022319232 Galaxea fascicularis|Galaxy coral|gfas1.m1.16337.m1 Crassostrea gigas|Pacific Oyster|XP 011456754 Porites lutea|Small polyp stony coral|plut2.m8.33025.m1 Crassostrea gigas|Pacific Oyster|XP 011456753 Amplexidiscus fenestrafer|Elephant ear anemone|evm.model.scaffold 66.49
Goniastrea aspera|Stony coral|gasp1.m3.27949.m1
Goniastrea aspera|Stony coral|gasp1.m3.27950.m1
Mizuhopecten yessoensis|Yesso scallop|XP 021340254 Fungia spp.|Coral|ffun1.m4.27540.m1
Aplysia californica|California sea hare|XP 012943366 Fungia spp.|Coral|ffun1.m4.29139.m1
Araneus ventricosus|Orb-weaver spider|GBM30498 Nematostella vectensis|Starlet sea anemone|jgi Nemve1 197088 fgenesh1 pg.scaffold 4000168
Aiptasia|Sea anemone|AIPGENE8755
Goniastrea aspera|Stony coral|gasp1.m3.24839.m1
Galaxea fascicularis|Galaxy coral|gfas1.m1.1188.m1
Stylophora pistillata|Hood coral|Spis19227
Fungia spp.|Coral|ffun1.m4.25767.m1 Parasteatoda tepidariorum|American house spider|XP 015904863 Amplexidiscus fenestrafer|Elephant ear anemone|evm.model.scaffold 66.48
Centruroides sculpturatus|Arizona bark scorpion|XP 023238515 Amplexidiscus fenestrafer|Elephant ear anemone|evm.model.scaffold 139.19
Nematostella vectensis|Starlet sea anemone|jgi Nemve1 31537 gw.8.57.1
Centruroides sculpturatus|Arizona bark scorpion|XP 023225280 Amplexidiscus fenestrafer|Elephant ear anemone|evm.model.scaffold 139.18
Amplexidiscus fenestrafer|Elephant ear anemone|evm.model.scaffold 66.47
Limulus polyphemus|American horseshoe crab|XP 013791075.2 Porites lutea|Small polyp stony coral|plut2.m8.25751.m1
Acropora digitifera|Coral|adi v1.03002 Limulus polyphemus|American horseshoe crab|AAN38979 Acropora digitifera|Coral|adi v1.09061
Parasteatoda tepidariorum|American house spider|XP 021002173 Acropora tenuis|Coral|aten 0.1.m1.11120.m1
Tropilaelaps mercedesae|Honey bee mite|OQR68673
Varroa jacobsoni|Asian honey bee mite|XP 022699356 Limulus polyphemus|American horseshoe crab|NP 001301020 Ixodes scapularis|Black-leggedIxodes scapularis|Black-legged tick|XP 029844767 tick|EEC03061 Varroa destructor|Honey bee mite|XP 022656459 Galendromus occidentalis|Western predatory mite|XP 018497405 Limulus polyphemus|American horseshoe crab|XP 022242800 Varroa destructor|Honey bee mite|XP 022656457 Varroa jacobsoni|Asian honey bee mite|XP 022699354
Aiptasia|Sea anemone|AIPGENE23973 Octopus vulgaris|Common octopus|XP 029648710 Nematostella vectensis|Starlet sea anemone|jgi Nemve1 92648 e gw.30.215.1 Acropora tenuis|Coral|aten 0.1.m1.10100.m1
Limulus polyphemus|American horseshoe crab|XP 013775532.2 Amplexidiscus fenestrafer|Elephant ear anemone|evm.model.scaffold 331.7
Pocillopora damicornis|Lace coral|pdam 00003294-RA Octopus vulgaris|Common octopus|XP 029648709 Stylophora pistillata|Hood coral|Spis10260
Galaxea fascicularis|Galaxy coral|gfas1.m1.2217.m1
Goniastrea aspera|Stony coral|gasp1.m3.11339.m1
Fungia spp.|Coral|ffun1.m4.11228.m1 Drosophila melanogaster|Common fruit fly|Trpl
Ixodes scapularis|Black-legged tick|XP 029844206
Centruroides sculpturatus|Arizona bark scorpion|XP 023230521 Limulus polyphemus|American horseshoe crab|XP 013773346
Parasteatoda tepidariorum|American house spider|XP 015930751 Drosophila melanogaster|Common fruit fly|Trp
Crassostrea gigas|Pacific Oyster|XP 019924985 Pomacea canaliculata|Golden apple snail|XP 025088295 Mizuhopecten yessoensis|Yesso scallop|XP 021376838
Crassostrea virginica|EasternBranchiostoma belcheri|Lancelet|XPoyster|XP 022321713 019647217
Branchiostoma belcheri|Lancelet|XP 019647218
Caenorhabditis elegans|Nematode|trp-1 ZC21.2b.1
Crassostrea virginica|Eastern oyster|XP 022302544 Crassostrea gigas|Pacific Oyster|XP 011420089 Crassostrea virginica|Eastern oyster|XP 022298027
Mizuhopecten yessoensis|Yesso scallop|XP 021364913 Tetranychus urticae XP 015791381
Tetranychus urticae XP 015788743
Limulus polyphemus|American horseshoe crab|XP 013791151.2
Drosophila melanogaster|Common fruit fly|Trpgamma Parasteatoda tepidariorum|American house spider|XP 015916803 Varroa destructor|Honey bee mite|XP 022649945 Centruroides sculpturatus|Arizona bark scorpion|XP 023238125 Parasteatoda tepidariorum|American house spider|XP 021001449 Ixodes scapularis|Black-legged tick|EEC04095 Limulus polyphemus|American horseshoe crab|XP 013776860
Limulus polyphemus|American horseshoe crab|NP 001301012 Dinothrombium tinctorium|Giant velvet mite|RWS07648
Ixodes scapularis|Black-legged tick|XP 029831199 Limulus polyphemus|American horseshoe crab|XP 022246931
Ixodes scapularis|Black-legged tick|XP 029828138 Varroa jacobsoni|Asian honey bee mite|XP 022708485 Tropilaelaps mercedesae|Honey bee mite|OQR79469
Leptotrombidium deliense|Chigger mite|RWS29242
Centruroides sculpturatus|Arizona bark scorpion|XP 023217963
Centruroides sculpturatus|Arizona bark scorpion|XP 023217962
Dinothrombium tinctorium|Giant velvet mite|RWS15299
Limulus polyphemus|American horseshoe crab|AIA22826
Galendromus occidentalis|Western predatory mite|XP 028968955 Centruroidessculpturatus|Arizona bark 023236287 scorpion|XP EP Branch Support
10 100
Figure S3 TRPS 100 Gene duplication Chordate TRPS 100 Priapulid TRPS 100 100 100 100 expansion Molluscan Myriapod TRPS 100 100 Tardigrade expansion expansion Nematode 100 100 100 96 99 expansion Chelicerate 100 100 100 100 96 100 100 100 100 95 95 99 98 99 100 97 100 100 100 100 100 99 99 96 100 98 100 100 100 100 100 100 100 100 100 100 100 100 100 99 98 99 100 100 100 100 99 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 99 98 Cnidarian TRPN Cnidarian TRPC Cnidarian TRPM Angiostrongylus costaricensis|Rat lungworm|VDM59274 Pristionchus pacificus|Nematode|PDM65852 Brugia malayi|Zoonotic lymphatic filarial roundworm|VIO90055 Brugia malayi|Zoonotic lymphatic filarial roundworm|VIO90056 Brugia pahangi|Zoonotic lymphatic filarial roundworm|VDN82751 Wuchereria bancrofti|Elephantiasis roundworm|VDM22829 Onchocerca ochengi|Bovine roundworm|VDK75875 Loa loa|Filarial eye roundworm|XP003136526 Acanthocheilonema viteae|Rodent filarial roundworm|VBB30520 Litomosoides sigmodontis|Filarial roundworm|VDM92201 Toxocara canis|Dog roundworm|KHN72908 Toxocara canis|Dog Roundworm|VDM38260 Anisakis simplex|Marine roundworm|VDK46643 Dracunculus medinensis|Guinea Worm|VDN59371 Priapulus caudatus|Penis worm|XP014670278 Crassostrea virginica|Eastern oyster|XP 022305861.1 Crassostrea virginica|Eastern oyster|XP 022313978.1 Crassostrea gigas|Pacific oyster|XP 019921765.1 Crassostrea gigas|Pacific oyster|XP 011443832.2 Crassostrea virginica|Eastern oyster|XP 022342149.1 Mizuhopecten yessoensis|Yesso scallop|XP 021360214.1 Biomphalaria glabrata|Freshwater snail|XP 013090849 Aplysia californica|California sea hare|XP012937912.1 Aplysia californica|California sea hare|XP012937083.1 Pomacea canaliculata|Golden apple snail|XP 025086702.1 Pomacea canaliculata|Golden apple snail|XP 025088552.1 Lottia gigantea|Owl limpet|XP009058724 Crassostrea gigas|Pacific oyster|XP 019925619.1 Crassostrea virginica|Eastern oyster|XP 022323164.1 Mizuhopecten yessoensis|Yesso scallop|OWF47104.1 Octopus bimaculoides|California two-spotoctopus|KOF70547 Octopus vulgaris|Common octopus|XP029638531.1 Branchiostoma floridae|Florida lacelet|XP 002600280 Branchiostoma belcheri|Lancelet|XP 019643532 Varroa destructor|Honey beemite|XP 022665230 Varroa jacobsoni|Asian honeybeemite|XP 022706112 Tropilaelaps mercedesae|Honey beemite|OQR67438 Galendromus occidentalis|Western predatory mite|XP018495285.2 Ixodes scapularis|Black-legged tick|XP029843579 Ixodes scapularis|Black-legged tick|XP029845457 Ixodes scapularis|Black-legged tick|XP029822890 Dermatophagoides pteronyssinus|House dustmite|XP 027194835 Euroglyphus maynei|House dustmite|OTF77807 Dermatophagoides pteronyssinus|House dustmite|XP 027204088 Dinothrombium tinctorium|Giant velvet mite|RWS12646 Leptotrombidium deliense|Chiggermite|RWS26931 Tetranychus urticae|Red spider mite|XP025016127 Araneus ventricosus|Orb-weaver spider|GBM00974 Trichonephila clavipes|Golden silkorb-weaver spider|PRD37692 Parasteatoda tepidariorum|American housespider|XP021001386 Centruroides sculpturatus|Arizona bark scorpion|XP023240584 Araneus ventricosus|Orb-weaver spider|GBN06084 Parasteatoda tepidariorum|American housespider|XP015912591 Centruroides sculpturatus|Arizona bark scorpion|XP023241337 Limulus polyphemus|American horseshoecrab|XP 022247894 Limulus polyphemus|American horseshoecrab|XP 022254350 Limulus polyphemus|American horseshoecrab|XP 022257243 Limulus polyphemus|American horseshoecrab|XP 022250999 Araneus ventricosus|Orb-weaver spider|GBM32255 Araneus ventricosus|Orb-weaver spider|GBM23289 Parasteatoda tepidariorum|American housespider|XP015907226 Araneus ventricosus|Orb-weaver spider|GBN13831 Parasteatoda tepidariorum|American housespider|XP015924394 Leptotrombidium deliense|Chiggermite|RWS28184 Dinothrombium tinctorium|Giant velvet mite|RWS01510 Leptotrombidium deliense|Chiggermite|RWS30779 Strigamia maritima|Centipede|SMAR010526-RA Strigamia maritima|Centipede|SMAR006477-RA Hypsibius dujardini|Tardigrade|OQV20964 Ramazzottius varieornatus|Tardigrade|GAU91171 Hypsibius dujardini|Tardigrade|OQV20961 Ramazzottius varieornatus|Tardigrade|GAU91175 Hypsibius dujardini|Tardigrade|OQV11764 Hypsibius dujardini|Tardigrade|OQV18852 Ramazzottius varieornatus|Tardigrade|GAV05896 Ramazzottius varieornatus|Tardigrade|GAU92779 Hypsibius dujardini|Tardigrade|OQV16369 Ramazzottius varieornatus|Tardigrade|GAU92603 Caenorhabditis remanei|Nematode|POM40678 Caenorhabditis remanei|Nematode|OZF75272 Caenorhabditis remanei|Nematode|XP 003112945 Caenorhabditis latens|Nematode|OZG18142 Caenorhabditis brenneri|Nematode|EGT33533 Caenorhabditis elegans|Nematode|ced-11 CE00409 Caenorhabditis briggsae|Nematode|XP 002642514 Caenorhabditis briggsae|Nematode|CAP27157.2 Caenorhabditis nigoni|Nematode|PIC39172 Haemonchus contortus|Barberspoleworm|CDJ89801 Haemonchus placei|Large stomach worm|VDO05215 Heligmosomoides polygyrus|Rodent intestinalroundworm|VDO61095 Ancylostoma ceylanicum|Hookworm|EYC40953 Oesophagostomum dentatum|Nodular worm|KHJ91906 Figure S4 Monophyletic Deuterostomia Xenambulacraria Hypothesis Hypothesis
Xenacoelomorpha Xenacoelomorpha
Protostomia Echinodermata
Echinodermata Hemichordata
Hemichordata Protostomia
Cephalochordata Cephalochordata
Craniata/Vertebrata Craniata/Vertebrata
Urochordata Urochordata
Figure S5 Arthropoda Chelicerata 100 Myriapoda 100
100 Crustacea
Hexapoda
Figure S6 100 TRPS Aurelia|Moon jelly|Seg1803.3 transcript id Seg1803.3|GoldUCD|mRNA.D3Y31 Hydra vulgaris|Fresh-water polyp|XP 012554388 100 Hydra vulgaris|Fresh-water polyp|XP 012562662 100 Hydra vulgaris|Fresh-water polyp|XP 012561335 Pocillopora damicornis|Lace coral|pdam 00007420-RA 99 Stylophora pistillata|Hood coral|Spis14035.t1 SpisGene14035 ? 100 Fungia spp.|Coral|ffun1.m4.21870.m1 Galaxea fascicularis|Galaxy coral|gfas1.m1.11287.m1 Goniastrea aspera|Stony coral|gasp1.m3.6245.m1 Saccoglossus kowalevskii|Acorn worm|XP 006816420 98 Acanthaster planci|Crown-of-thorns starfish|XP 022096576.1 99 Strongylocentrotus purpuratus|Purple sea urchin|XP 011661004.1 Acanthaster planci|Crown-of-thorns starfish|XP 022086910.1 Ptychodera flava|Acorn worm|pfl 40v0 9 20150316 1g5514. Ptychodera flava|Acorn worm|pfl 40v0 9 20150316 1g24145. Praesagittifera naikaiensis|Acoel flatworm|g40739.t1 Hofstenia miamia|Three-banded panther worm|scaffold112 437721 469571 R 100 Hofstenia miamia|Three-banded panther worm|scaffold18 807971 810976 F 100 Hofstenia miamia|Three-banded panther worm|scaffold18 866412 869501 F 100 Praesagittifera naikaiensis|Acoel flatworm|g36760.t1 Praesagittifera naikaiensis|Acoel flatworm|g4935.t1 100 Praesagittifera naikaiensis|Acoel flatworm|g11490.t1 98 Praesagittifera naikaiensis|Acoel flatworm|g16021.t1 98 100 Hofstenia miamia|Three-banded panther worm|scaffold488 5372 8923 F Hofstenia miamia|Three-banded panther worm|scaffold387 196851 200279 F 96 Hofstenia miamia|Three-banded panther worm|scaffold387 254022 257456 F 100 99 Hofstenia miamia|Three-banded panther worm|scaffold387 247539 251000 F 99 Hofstenia miamia|Three-banded panther worm|scaffold387 219858 223314 F 100 Hofstenia miamia|Three-banded panther worm|scaffold391 331241 359300 R 100 100 Hofstenia miamia|Three-banded panther worm|scaffold387 211324 251000 F Praesagittifera naikaiensis|Acoel flatworm|g25700.t1 Saccoglossus kowalevskii|Acorn worm|XP 006814003 100 100 Strongylocentrotus purpuratus|Purple sea urchin|XP 794425.3 100 Acanthaster planci|Crown-of-thorns starfish|XP 022099673.1 98 Drosophila melanogaster|Common fruit fly|Trpm α Homo sapiens|Human|TRPM3 CCDS6634.1 99 100 Homo sapiens|Human|TRPM1 CCDS10024.2 100 Homo sapiens|Human|TRPM7 CCDS42035.1 100 Homo sapiens|Human|TRPM6 CCDS6647.1 Aiptasia|Sea anemone|AIPGENE12924 100 Nematostella vectensis|Starlet sea anemone|Nemve1|85952 99 Pocillopora damicornis|Lace coral|pdam 00014050-RA 100 Goniastrea aspera|Stony coral|gasp1.m3.26670.m1 99 Fungia spp.|Coral|ffun1.m4.18855.m1 100 Aiptasia|Sea anemone|AIPGENE19094 100 Nematostella vectensis|Starlet sea anemone|Nemve1|102167 Porites lutea|Small polyp stony coral|plut2.m8.21682.m1 Pocillopora damicornis|Lace coral|pdam 00020220-RA 100 99 Goniastrea aspera|Stony coral|gasp1.m3.6037.m1 98 Fungia spp.|Coral|ffun1.m4.18290.m1 Hydra vulgaris|Fresh-water polyp|XP 012565257 99 Aurelia|Moon jelly|Seg1831.8 transcript id Seg1831.8|GoldUCD|mRNA.D3Y31 Nematostella vectensis|Starlet sea anemone|Nemve1|248535 100 Pocillopora damicornis|Lace coral|pdam 00009856-RA 100 100 Stylophora pistillata|Hood coral|Spis4675 SpisGene4675 β Fungia spp.|Coral|ffun1.m4.19256.m1 100 98 Goniastrea aspera|Stony coral|gasp1.m3.30302.m1 96 Porites lutea|Small polyp stony coral|plut2.m8.2561.m1 Galaxea fascicularis|Galaxy coral|gfas1.m1.6599.m1 100 99 100 Acropora digitifera|Coral|adi v1.10270 100 Acropora tenuis|Coral|aten 0.1.m1.1851.m1 Praesagittifera naikaiensis|Acoel flatworm|g14495.t1 100 Praesagittifera naikaiensis|Acoel flatworm|g14113.t1 100 Praesagittifera naikaiensis|Acoel flatworm|g10675.t1 Homo sapiens|Human|TRPM8 CCDS33407.1 100 Homo sapiens|Human|TRPM2 CCDS13710.1 100 Homo sapiens|Human|TRPM5 CCDS31340.1 100 Homo sapiens|Human|TRPM4 CCDS33073.1 Ptychodera flava|Acorn worm|pfl 40v0 9 20150316 1g7524. Ptychodera flava|Acorn worm|pfl 40v0 9 20150316 1g8370. 100 100 Ptychodera flava|Acorn worm|pfl 40v0 9 20150316 1g8628. 100 Ptychodera flava|Acorn worm|pfl 40v0 9 20150316 1g26243. Strongylocentrotus purpuratus|Purple sea urchin|XP 011664673.1 Apostichopus japonicus|Japanese sea cucumber|PIK58237.1 100 Acanthaster planci|Crown-of-thorns starfish|XP 022110675.1 100 Acanthaster planci|Crown-of-thorns starfish|XP 022110674.1 100 Strongylocentrotus purpuratus|Purple sea urchin|XP 011674872.1 99 Apostichopus japonicus|Japanese sea cucumber|PIK46475.1 100 Acanthaster planci|Crown-of-thorns starfish|XP 022096929.1 100 Acanthaster planci|Crown-of-thorns starfish|XP 022084590.1 100 Acanthaster planci|Crown-of-thorns starfish|XP 022084599.1
Duplications before Cniadaria-Bilateria split Figure S7 100 TRPS Hydra vulgaris|Fresh-water polyp|XP 012554388 Hydra vulgaris|Fresh-water polyp|XP 012562662 100 100 Hydra vulgaris|Fresh-water polyp|XP 012561335 Pocillopora damicornis|Lace coral|pdam 00007420-RA 99 Stylophora pistillata|Hood coral|Spis14035.t1 SpisGene14035 100 Fungia spp.|Coral|ffun1.m4.21870.m1 Galaxea fascicularis|Galaxy coral|gfas1.m1.11287.m1 β Goniastrea aspera|Stony coral|gasp1.m3.6245.m1 Aiptasia|Sea anemone|AIPGENE12924 Nematostella vectensis|Starlet sea anemone|Nemve1|85952 Pocillopora damicornis|Lace coral|pdam 00014050-RA Goniastrea aspera|Stony coral|gasp1.m3.26670.m1 100 100 Fungia spp.|Coral|ffun1.m4.18855.m1 100 Aiptasia|Sea anemone|AIPGENE19094 100 Nematostella vectensis|Starlet sea anemone|Nemve1|102167 99 Porites lutea|Small polyp stony coral|plut2.m8.21682.m1 Pocillopora damicornis|Lace coral|pdam 00020220-RA 100 Fungia spp.|Coral|ffun1.m4.18290.m1 99 Goniastrea aspera|Stony coral|gasp1.m3.6037.m1 Praesagittifera naikaiensis|Acoel flatworm|g40739.t1 Praesagittifera naikaiensis|Acoel flatworm|g14495.t1 Praesagittifera naikaiensis|Acoel flatworm|g14113.t1 98 100 Praesagittifera naikaiensis|Acoel flatworm|g10675.t1 Ciona intestinalis|Vase tunicate|XP 026692588 Oikopleura dioica|Tunicate|CBY19337 Oikopleura dioica|Tunicate|CBY13016 100 Oikopleura dioica|Tunicate|CBY10098 Branchiostoma floridae|Florida lancelet|XP 002592165 Branchiostoma belcheri|Lancelet|XP 019638014 Branchiostoma floridae|Florida lancelet|XP 002605080 100 Branchiostoma floridae|Florida lancelet|XP 002592248 99 100 Branchiostoma belcheri|Lancelet|XP 019640471 TRPM8 Latimeria chalumnae|Coelacanth|XP 014345846.1 Gallus gallus|Chicken|TRPM8 E1BWL7 CHICK 100 Mus musculus|Mouse|Trpm8 CCDS48316.1 100 100 Homo sapiens|Human|TRPM8 CCDS33407.1 Latimeria chalumnae|Coelacanth|XP 014345762.1 100 Gallus gallus|Chicken|TRPM2 F1NGK6 CHICK 100 Mus musculus|Mouse|Trpm2 CCDS48611.1 100 100 Homo sapiens|Human|TRPM2 CCDS13710.1 100 Callorhinchus milii|Ghostshark|XP 007888802.1 TRPM2 100 Callorhinchus milii|Ghostshark|XP 007888801.1 100 Scyliorhinus torazame|Cloudy catshark|Scyto0014884.p1 Chiloscyllium punctatum|Brownbanded bamboo shark|Chipu0018262.p1 100 100 100 100 Rhincodon typus|Whale shark|XP 020374811.1 TRPM4 Scyliorhinus torazame|Cloudy catshark|Scyto0007372.p1 Latimeria chalumnae|Coelacanth|XP 005990988.1 100 Mus musculus|Mouse|Trpm4 CCDS52245.1 100 Homo sapiens|Human|TRPM4 CCDS33073.1 Latimeria chalumnae|Coelacanth|XP 014344856.1 100 Gallus gallus|Chicken|TRPM5 E1BXA4 CHICK 100 Mus musculus|Mouse|Trpm5 CCDS52462.1 100 100 Homo sapiens|Human|TRPM5 CCDS31340.1 100 Callorhinchus milii|Ghostshark|XP 007902997.1 Rhincodon typus|Whale shark|XP 020389255.1 TRPM5 98 Chiloscyllium punctatum|Brownbanded bamboo shark|Chipu0004516.p1 100 Carcharodon carcharias|Great white shark|ScRDnwc 40374 6376172-snap-gene-36.2-mRNA-1 100 100 Scyliorhinus torazame|Cloudy catshark|Scyto0000828.p1 Aurelia|Moon jelly|Seg1803.3 transcript id Seg1803.3|GoldUCD|mRNA.D3Y31 Hofstenia miamia|Three-banded panther worm|scaffold112 437721 469571 R Hofstenia miamia|Three-banded panther worm|scaffold18 807971 810976 F 97 100 100 Hofstenia miamia|Three-banded panther worm|scaffold18 866412 869501 F Praesagittifera naikaiensis|Acoel flatworm|g16021.t1 100 100 Hofstenia miamia|Three-banded panther worm|scaffold488 5372 8923 F Praesagittifera naikaiensis|Acoel flatworm|g11490.t1 Praesagittifera naikaiensis|Acoel flatworm|g36760.t1 ? 100 Praesagittifera naikaiensis|Acoel flatworm|g4935.t1 Hofstenia miamia|Three-banded panther worm|scaffold387 196851 200279 F Hofstenia miamia|Three-banded panther worm|scaffold387 254022 257456 F 100 Hofstenia miamia|Three-banded panther worm|scaffold387 247539 251000 F 99 Hofstenia miamia|Three-banded panther worm|scaffold387 219858 223314 F Hofstenia miamia|Three-banded panther worm|scaffold391 331241 359300 R 100 100 Hofstenia miamia|Three-banded panther worm|scaffold387 211324 251000 F Hydra vulgaris|Fresh-water polyp|XP 012565257 97 Aurelia|Moon jelly|Seg1831.8 transcript id Seg1831.8|GoldUCD|mRNA.D3Y31 Nematostella vectensis|Starlet sea anemone|Nemve1|248535 100 Porites lutea|Small polyp stony coral|plut2.m8.2561.m1 Galaxea fascicularis|Galaxy coral|gfas1.m1.6599.m1 99 100 Acropora digitifera|Coral|adi v1.10270 100 α 100 Acropora tenuis|Coral|aten 0.1.m1.1851.m1 100 Fungia spp.|Coral|ffun1.m4.19256.m1 100 Goniastrea aspera|Stony coral|gasp1.m3.30302.m1 Pocillopora damicornis|Lace coral|pdam 00009856-RA 100 Stylophora pistillata|Hood coral|Spis4675 SpisGene4675 Drosophila melanogaster|Common fruit fly|Trpm Praesagittifera naikaiensis|Acoel flatworm|g25700.t1 Ciona intestinalis|Vase tunicate|XP 018667443 100 Branchiostoma lanceolatum|European lacelet|BL24111 evm0 96 100 Branchiostoma belcheri|Lancelet|XP 019640450 Chiloscyllium punctatum|Brownbanded bamboo shark|Chipu0016510.p1 TRPM3 100 Callorhinchus milii|Ghostshark|XP 007904772.1 100 Latimeria chalumnae|Coelacanth|XP 014347481.1 Gallus gallus|Chicken|TRPM3 F1NJZ7 CHICK 100 Mus musculus|Mouse|Trpm3 CCDS29702.1 100 Homo sapiens|Human|TRPM3 CCDS6634.1 100 100 95 Eptatretus burgeri|Inshore hagfish|trpm1b-201|ENSEBUP00000012388 Latimeria chalumnae|Coelacanth|XP 014348116.1 Gallus gallus|Chicken|TRPM1 F1NCK1 CHICK 100 100 Mus musculus|Mouse|TRPM1 CCDS21332.2 TRPM1 100 100 Homo sapiens|Human|TRPM1 CCDS10024.2 100 Callorhinchus milii|Ghostshark|XP 007907039.1 Rhincodon typus|Whale shark|XP 020377793.1 100 Carcharodon carcharias|Great white shark|ScRDnwc 41824 761978-snap-gene-2.12-mRNA-1 100 100 100 Chiloscyllium punctatum|Brownbanded bamboo shark|Chipu0014745.pl Callorhinchus milii|Ghostshark|XP 007897564.1 Scyliorhinus torazame|Cloudy catshark|Scyto0003223.p1 100 TRPM6 100 Chiloscyllium punctatum|Brownbanded bamboo shark|Chipu0003513.p1 100 Latimeria chalumnae|Coelacanth|XP 014349563.1 Gallus gallus|Chicken|TRPM6 E1C5L2 CHICK 100 Mus musculus|Mouse|Trpm6 CCDS29691.1 100 100 100 Homo sapiens|Human|TRPM6 CCDS6647.1 Petromyzon marinus|Sea lamprey|PMZ 0026066-RA Latimeria chalumnae|Coelacanth|XP 005989708.1 Gallus gallus|Chicken|TRPM7 E1BYV3 CHICK 99 100 Mus musculus|Mouse|Trpm7 CCDS16689.1 100 TRPM7 100 Homo sapiens|Human|TRPM7 CCDS42035.1 100 Callorhinchus milii|Ghostshark|XP 007903830.1 Chiloscyllium punctatum|Brownbanded bamboo shark|Chipu0017399.p1 100 100 Rhincodon typus|Whale shark|XP 020370945.1 100 Carcharodon carcharias|Great white shark|ScRDnwc 11009 1186063-snap-gene-4.2-mRNA-1 100 Scyliorhinus torazame|Cloudy catshark|Scyto0018498.p1
Duplications before Cniadaria-Bilateria split Figure S8 100 TRPS Hydra vulgaris|Fresh-water polyp|XP 012565257 98 Aurelia|Moon jelly|Seg1831.8 transcript id Seg1831.8|GoldUCD|mRNA.D3Y31 Nematostella vectensis|Starlet sea anemone|Nemve1|248535 100 Fungia spp.|Coral|ffun1.m4.19256.m1 Goniastrea aspera|Stony coral|gasp1.m3.30302.m1 100 99 Pocillopora damicornis|Lace coral|pdam 00009856-RA 100 Stylophora pistillata|Hood coral|Spis4675 SpisGene4675 β 100 Porites lutea|Small polyp stony coral|plut2.m8.2561.m1 Galaxea fascicularis|Galaxy coral|gfas1.m1.6599.m1 98 Acropora digitifera|Coral|adi v1.10270 98 100 Acropora tenuis|Coral|aten 0.1.m1.1851.m1 95 Praesagittifera naikaiensis|Acoel flatworm|g14495.t1 Praesagittifera naikaiensis|Acoel flatworm|g14113.t1 100 100 Praesagittifera naikaiensis|Acoel flatworm|g10675.t1 Homo sapiens|Human|TRPM8 CCDS33407.1 100 Homo sapiens|Human|TRPM2 CCDS13710.1 100 Homo sapiens|Human|TRPM5 CCDS31340.1 100 Homo sapiens|Human|TRPM4 CCDS33073.1 Capitella teleta|Annelid worm|ELT91987.1 Capitella teleta|Annelid worm|ELT97859.1 100 100 Capitella teleta|Annelid worm|ELT96530.1 Helobdella robusta|Leech|XP 009025643.1 100 Capitella teleta|Annelid worm|ELT97299.1 Notospermus geniculatus|Ribbon worm|g1465.t1 Notospermus geniculatus|Ribbon worm|g14995.t1 100 100 100 Notospermus geniculatus|Ribbon worm|g29200.t1 Lingula anatina|Brachiopod|XP 013384361.1 Phoronis australis|Horseshoe worm|g10339.t1 Mizuhopecten yessoensis|Yesso scallop|OWF37798.1 Crassostrea virginica|Eastern oyster|XP 022319512.1 100 Crassostrea gigas|Pacific oyster|XP 011414360.1 100 100 Crassostrea gigas|Pacific oyster|XP 011456090.1 100 Pomacea canaliculata|Golden apple snail|PVD39019.1 100 Pomacea canaliculata|Golden apple snail|XP 025089091.1 100 Biomphalaria glabrata|Freshwater snail|XP 013061400.1 Aplysia californica|California sea hare|XP 005091977.2 100 100 Biomphalaria glabrata|Freshwater snail|XP 013061392.1 Lingula anatina|Brachiopod|XP 013398789.1 Phoronis australis|Horseshoe worm|g2633.t1 100 Helobdella robusta|Leech|XP 009013475.1 100 100 Capitella teleta|Annelid worm|ELU00429.1 Octopus vulgaris|Common octopus|XP 029647149.1 Octopus vulgaris|Common octopus|XP 029646813.1 100 100 Octopus bimaculoides|California two-spot octopus|XP 014781149.1 100 100 Octopus vulgaris|Common octopus|XP 029646968.1 Lottia gigantea|Owl limpet|XP 009055754.1 Pomacea canaliculata|Golden apple snail|PVD35972.1 100 Pomacea canaliculata|Golden apple snail|XP 025081224.1 100 99 Aplysia californica|California sea hare|XP 012942949.1 99 Biomphalaria glabrata|Freshwater snail|XP 013063620.1 100 Aplysia californica|California sea hare|XP 005100068.2 100 Aplysia californica|California sea hare|XP 012940727.1 Mizuhopecten yessoensis|Yesso scallop|XP 021362498.1 99 Crassostrea virginica|Eastern oyster|XP 022336960.1 98 Crassostrea gigas|Pacific oyster|XP 019924531.1 100 Crassostrea gigas|Pacific oyster|XP 011433270.1 Crassostrea gigas|Pacific oyster|XP 019924535.1 Mizuhopecten yessoensis|Yesso scallop|XP 021375672.1 100 Mizuhopecten yessoensis|Yesso scallop|XP 021375666.1 Crassostrea gigas|Pacific oyster|XP 011446980.2 100 Crassostrea virginica|Eastern oyster|XP 022342892.1 97 Crassostrea virginica|Eastern oyster|XP 022338390.1 100 Crassostrea virginica|Eastern oyster|XP 022339354.1 Fungia spp.|Coral|ffun1.m4.21870.m1 Galaxea fascicularis|Galaxy coral|gfas1.m1.11287.m1 100 Goniastrea aspera|Stony coral|gasp1.m3.6245.m1 Pocillopora damicornis|Lace coral|pdam 00007420-RA 100 100 Stylophora pistillata|Hood coral|Spis14035.t1 SpisGene14035 Lingula anatina|Brachiopod|XP 023931219.1 100 Capitella teleta|Annelid worm|ELT87598.1 ? Pomacea canaliculata|Golden apple snail|PVD30001.1 Aplysia californica|California sea hare|XP 005094347.1 98 100 100 Biomphalaria glabrata|Freshwater snail|XP 013090385.1 98 Crassostrea gigas|Pacific oyster|XP 019919132.1 100 100 Crassostrea virginica|Eastern oyster|XP 022340640.1 97 Mizuhopecten yessoensis|Yesso scallop|XP 021378397.1 Mizuhopecten yessoensis|Yesso scallop|XP 021352500.1 Aurelia|Moon jelly|Seg1803.3 transcript id Seg1803.3|GoldUCD|mRNA.D3Y31 Hofstenia miamia|Three-banded panther worm|scaffold112 437721 469571 R Hofstenia miamia|Three-banded panther worm|scaffold18 807971 810976 F 100 99 100 Hofstenia miamia|Three-banded panther worm|scaffold18 866412 869501 F Praesagittifera naikaiensis|Acoel flatworm|g16021.t1 100 Hofstenia miamia|Three-banded panther worm|scaffold488 5372 8923 F 100 99 Praesagittifera naikaiensis|Acoel flatworm|g11490.t1 Praesagittifera naikaiensis|Acoel flatworm|g36760.t1 98 Praesagittifera naikaiensis|Acoel flatworm|g4935.t1 100 Hofstenia miamia|Three-banded panther worm|scaffold387 196851 200279 F Hofstenia miamia|Three-banded panther worm|scaffold387 254022 257456 F 99 Hofstenia miamia|Three-banded panther worm|scaffold387 247539 251000 F 100 Hofstenia miamia|Three-banded panther worm|scaffold387 219858 223314 F Hofstenia miamia|Three-banded panther worm|scaffold391 331241 359300 R 100 100 Hofstenia miamia|Three-banded panther worm|scaffold387 211324 251000 F Hydra vulgaris|Fresh-water polyp|XP 012554388 Hydra vulgaris|Fresh-water polyp|XP 012562662 100 100 Hydra vulgaris|Fresh-water polyp|XP 012561335 Aiptasia|Sea anemone|AIPGENE12924 100 Nematostella vectensis|Starlet sea anemone|Nemve1|85952 99 Pocillopora damicornis|Lace coral|pdam 00014050-RA α Goniastrea aspera|Stony coral|gasp1.m3.26670.m1 100 98 Fungia spp.|Coral|ffun1.m4.18855.m1 97 Aiptasia|Sea anemone|AIPGENE19094 100 Nematostella vectensis|Starlet sea anemone|Nemve1|102167 Porites lutea|Small polyp stony coral|plut2.m8.21682.m1 100 97 Pocillopora damicornis|Lace coral|pdam 00020220-RA 100 Goniastrea aspera|Stony coral|gasp1.m3.6037.m1 97 100 Fungia spp.|Coral|ffun1.m4.18290.m1 Praesagittifera naikaiensis|Acoel flatworm|g40739.t1 Praesagittifera naikaiensis|Acoel flatworm|g25700.t1 Homo sapiens|Human|TRPM3 CCDS6634.1 100 Homo sapiens|Human|TRPM1 CCDS10024.2 100 100 Homo sapiens|Human|TRPM7 CCDS42035.1 100 Homo sapiens|Human|TRPM6 CCDS6647.1 Drosophila melanogaster|Common fruit fly|Trpm Helobdella robusta|Leech|XP 009015939.1 Helobdella robusta|Leech|XP 009013900.1 100 Helobdella robusta|Leech|XP 009009645.1 Notospermus geniculatus|Ribbon worm|g23156.t1 Lingula anatina|Brachiopod|XP 013397522.1 Phoronis australis|Horseshoe worm|g14558.t1 Octopus vulgaris|Common octopus|XP 029641654.1 100 Octopus bimaculoides|California two-spot octopus|XP 014787598.1 98 Aplysia californica|California sea hare|XP 012936450.1 Biomphalaria glabrata|Freshwater snail|XP 013083453.1 100 100 Biomphalaria glabrata|Freshwater snail|XP 013083410.1 100 Mizuhopecten yessoensis|Yesso scallop|OWF43174.1 100 Mizuhopecten yessoensis|Yesso scallop|XP 021368426.1 Mizuhopecten yessoensis|Yesso scallop|XP 021372912.1 100 Crassostrea gigas|Pacific oyster|XP 011435194.1 97 100 Crassostrea gigas|Pacific oyster|XP 011435204.1 99 Crassostrea gigas|Pacific oyster|XP 011414379.1 100 Crassostrea virginica|Eastern oyster|XP 022295841.1 Duplications before Cniadaria-Bilateria split Figure S9 100 TRPS Toxocara canis|Dog roundworm|VDM40999 100 Toxocara canis|Dog roundworm|KHN83770 Praesagittifera naikaiensis|Acoel flatworm|g14495.t1 Praesagittifera naikaiensis|Acoel flatworm|g14113.t1 99 100 Praesagittifera naikaiensis|Acoel flatworm|g10675.t1 Hydra vulgaris|Fresh-water polyp|XP 012554388 Hydra vulgaris|Fresh-water polyp|XP 012562662 100 100 Hydra vulgaris|Fresh-water polyp|XP 012561335 Galaxea fascicularis|Galaxy coral|gfas1.m1.11287.m1 Fungia spp.|Coral|ffun1.m4.21870.m1 100 ? Goniastrea aspera|Stony coral|gasp1.m3.6245.m1 96 Pocillopora damicornis|Lace coral|pdam 00007420-RA 97 97 Stylophora pistillata|Hood coral|Spis14035.t1 SpisGene14035 Praesagittifera naikaiensis|Acoel flatworm|g40739.t1 Priapulus caudatus|Penis worm|XP 014669422 Homo sapiens|Human|TRPM8 CCDS33407.1 95 100 Homo sapiens|Human|TRPM2 CCDS13710.1 100 Homo sapiens|Human|TRPM5 CCDS31340.1 100 100 Homo sapiens|Human|TRPM4 CCDS33073.1 Hydra vulgaris|Fresh-water polyp|XP 012565257 98 Aurelia|Moon jelly|Seg1831.8 transcript id Seg1831.8|GoldUCD|mRNA.D3Y31 Nematostella vectensis|Starlet sea anemone|Nemve1|248535 100 Fungia spp.|Coral|ffun1.m4.19256.m1 Goniastrea aspera|Stony coral|gasp1.m3.30302.m1 β 100 100 Pocillopora damicornis|Lace coral|pdam 00009856-RA 100 Stylophora pistillata|Hood coral|Spis4675 SpisGene4675 100 Porites lutea|Small polyp stony coral|plut2.m8.2561.m1 100 Galaxea fascicularis|Galaxy coral|gfas1.m1.6599.m1 Acropora digitifera|Coral|adi v1.10270 100 Acropora tenuis|Coral|aten 0.1.m1.1851.m1 Aurelia|Moon jelly|Seg1803.3 transcript id Seg1803.3|GoldUCD|mRNA.D3Y31 Hofstenia miamia|Three-banded panther worm|scaffold112 437721 469571 R Hofstenia miamia|Three-banded panther worm|scaffold18 807971 810976 F 100 99 100 Hofstenia miamia|Three-banded panther worm|scaffold18 866412 869501 F Praesagittifera naikaiensis|Acoel flatworm|g16021.t1 100 100 Hofstenia miamia|Three-banded panther worm|scaffold488 5372 8923 F Praesagittifera naikaiensis|Acoel flatworm|g36760.t1
100 Praesagittifera naikaiensis|Acoel flatworm|g11490.t1 Praesagittifera naikaiensis|Acoel flatworm|g4935.t1 Hofstenia miamia|Three-banded panther worm|scaffold387 254022 257456 F 97 Hofstenia miamia|Three-banded panther worm|scaffold387 247539 251000 F ?
100 Hofstenia miamia|Three-banded panther worm|scaffold387 196851 200279 F Hofstenia miamia|Three-banded panther worm|scaffold387 219858 223314 F 100 Hofstenia miamia|Three-banded panther worm|scaffold391 331241 359300 R 100 100 Hofstenia miamia|Three-banded panther worm|scaffold387 211324 251000 F Aiptasia|Sea anemone|AIPGENE12924 100 Nematostella vectensis|Starlet sea anemone|Nemve1|85952 Pocillopora damicornis|Lace coral|pdam 00014050-RA Fungia spp.|Coral|ffun1.m4.18855.m1 100 Goniastrea aspera|Stony coral|gasp1.m3.26670.m1 100 Aiptasia|Sea anemone|AIPGENE19094 100 Nematostella vectensis|Starlet sea anemone|Nemve1|102167
98 Pocillopora damicornis|Lace coral|pdam 00020220-RA Porites lutea|Small polyp stony coral|plut2.m8.21682.m1 α 100 Goniastrea aspera|Stony coral|gasp1.m3.6037.m1 95 100 Fungia spp.|Coral|ffun1.m4.18290.m1 Praesagittifera naikaiensis|Acoel flatworm|g25700.t1 Homo sapiens|Human|TRPM3 CCDS6634.1 100 Homo sapiens|Human|TRPM1 CCDS10024.2 99 Homo sapiens|Human|TRPM7 CCDS42035.1 100 100 Homo sapiens|Human|TRPM6 CCDS6647.1 Priapulus caudatus|Penis worm|XP 014671784 Drosophila melanogaster|Common fruit fly|Trpm Trichuris trichiura|Human whipworm|CDW57881 100 Trichuris suis|Whipworm|KFD68196 Trichuris trichiura|Human whipworm|CDW55439 Trichuris suis|Whipworm|KHJ40964 100 100 Trichuris suis|Whipworm|KFD50001 Trichinella zimbabwensis|Trichinella roundworm|KRZ13952 Trichinella zimbabwensis|Trichinella roundworm|KRZ13953 Trichinella papuae|Swine roundworm|KRZ71139 Trichinella papuae|Swine roundworm|KRZ71140 Trichinella papuae|Swine roundworm|KRZ71142 Trichinella papuae|Swine roundworm|KRZ71141
100 Trichinella pseudospiralis|Trichinella roundworm|KRX87365 Trichinella pseudospiralis|Trichinella roundworm|KRX87370 Trichinella pseudospiralis|Trichinella roundworm|KRX87369 Trichinella pseudospiralis|Trichinella roundworm|KRY69106 Trichinella pseudospiralis|Trichinella roundworm|KRX87366 Trichinella pseudospiralis|Trichinella roundworm|KRY69105 Trichinella pseudospiralis|Trichinella roundworm|KRY69104 Trichinella pseudospiralis|Trichinella roundworm|KRY89154
98 Trichinella pseudospiralis|Trichinella roundworm|KRZ15873 Trichinella pseudospiralis|Trichinella roundworm|KRX87367
99 Trichinella patagoniensis|Trichinella roundworm|KRY10753 Trichinella patagoniensis|Trichinella roundworm|KRY10755 Trichinella patagoniensis|Trichinella roundworm|KRY10754 Trichinella spiralis|Trichinella roundworm|KRY38903 Trichinella nelsoni|Trichinella roundworm|KRX17502 Trichinella nelsoni|Trichinella roundworm|KRX17501 Trichinella spiralis|Trichinella roundworm|XP 003374666 Trichinella spiralis|Trichinella roundworm|KRY38904 Trichinella sp. T8|Trichinella roundworm|KRZ87599 100 Trichinella sp. T9|Trichinella roundworm|KRX59745 Trichinella sp. T6|Trichinella roundworm|KRX79063 Trichinella murrelli|Trichinella roundworm|KRX41628 Trichinella sp. T6|Trichinella roundworm|KRX79064 Trichinella murrelli|Trichinella roundworm|KRX41627 Trichinella murrelli|Trichinella roundworm|KRX41631 98 Trichinella murrelli|Trichinella roundworm|KRX41626 Trichinella britovi|Trichinella roundworm|KRY53687 Trichinella nativa|Trichinella roundworm|KRZ52384 Trichinella britovi|Trichinella roundworm|KRY53688 Trichinella nativa|Trichinella roundworm|KRZ52381 Trichinella britovi|Trichinella roundworm|KRY53684 100 Trichinella nativa|Trichinella roundworm|KRZ52382 Trichinella britovi|Trichinella roundworm|KRY53685 Trichinella nativa|Trichinella roundworm|KRZ52383 Trichinella nativa|Trichinella roundworm|KRZ52380 Strongyloides ratti|Rat roundworm|XP 024504814 100 Steinernema carpocapsae|Entomopathogenic roundworm|TKR95141 Pristionchus pacificus|Nematode|PDM69563 Teladorsagia circumcincta|Brown stomach worm|PIO69036 Dictyocaulus viviparus|Lungworm|KJH43833 100 gtl-2 100 Ancylostoma caninum|Dog hookworm|RCN33658 100 100 100 Ancylostoma ceylanicum|Hookworm|EYC46005 100 Diploscapter pachys|Nematode|PAV61018 Caenorhabditis latens|Nematode|OZF91865 Caenorhabditis remanei|Nematode|OZF99260 98 100 100 Caenorhabditis remanei|Nematode|XP 003107995 95 100 Caenorhabditis elegans|Nematode|gtl-2 CE40563 Caenorhabditis nigoni|Nematode|PIC34278 99 Caenorhabditis briggsae|Nematode|CAP26317.2 100 100 Caenorhabditis briggsae|Nematode|XP 002633316 Dracunculus medinensis|Guinea worm|VDN58044 Anisakis simplex|Marine roundworm|VDK42691 Toxocara canis|Dog roundworm|KHN79092 100 100 Toxocara canis|Dog roundworm|VDM44510 Thelazia callipaeda|Eye roundworm|VDN01629 Acanthocheilonema viteae|Rodent filarial roundworm|VBB25615 100 Loa loa|Filarial eye roundworm|XP 020302521 Onchocerca ochengi|Bovine roundworm|VDK63290 Onchocerca flexuosa|River blindness eye roundworm| VDP13005 100 Onchocerca flexuosa|River blindness eye roundworm|OZC12680 Wuchereria bancrofti|Elephantiasis roundworm|VDM18860 100 Brugia pahangi|Zoonotic lymphatic filarial roundworm|VDN83289 Brugia timori|Timor roundworm|VDO30096 Brugia malayi|Elephantiasis roundworm|CDQ02415 Brugia malayi|Elephantiasis roundworm|XP 001901231 Ancylostoma ceylanicum|Hookworm|EPB67786 100 Ancylostoma ceylanicum|Hookworm|EYB91093 100 Nippostrongylus brasiliensis|Rat hookworm|VDL83792 gtl-1 100 Haemonchus placei|Large stomach worm|VDO51628 Pristionchus pacificus|Nematode|PDM75278 100 100 Pristionchus pacificus|Nematode|PDM64775 Diploscapter pachys|Nematode|PAV79744 100 Diploscapter pachys|Nematode|PAV84897 Caenorhabditis elegans|Nematode|gtl-1 CE33754 100 Caenorhabditis brenneri|Nematode|EGT50227 Caenorhabditis remanei|Nematode|POM45300 100 Caenorhabditis remanei|Nematode|XP 003107819 Caenorhabditis briggsae|Nematode|CAP26324.2 Caenorhabditis briggsae|Nematode|XP 002633309 96 Caenorhabditis nigoni|Nematode|PIC34271 96 Caenorhabditis nigoni|Nematode|PIC34272 Ancylostoma caninum|Dog hookworm|RCN42281 100 Ancylostoma ceylanicum|Hookworm|EYC21527 Dictyocaulus viviparus|Lungworm|KJH53513 100 99 Angiostrongylus costaricensis|Rat lungworm|VDM52884 Nippostrongylus brasiliensis|Rat hookworm|VDL62851 95 98 Heligmosomoides polygyrus|Rodent intestinal roundworm|VDO88774 95 Haemonchus placei|Large stomach worm|VDO45564 100 Haemonchus contortus|Barber s pole worm|CDJ90594 Enterobius vermicularis|Human pinworm|VDD91528 Dracunculus medinensis|Guinea worm|VDN56541 gon-2 100 Anisakis simplex|Marine roundworm|VDK52174 100 Toxocara canis|Dog roundworm|KHN71877 Thelazia callipaeda|Thelizia eye worm|VDN07735 Acanthocheilonema viteae|Rodent filarial roundworm|VBB32995 100 Loa loa|Filarial eye roundworm|XP 020305655 96 Wuchereria bancrofti|Elephantiasis roundworm|VDM09111 99 Brugia pahangi|Zoonotic lymphatic filarial roundworm|VDN94084 97 Brugia malayi|Elephantiasis roundworm|VIO96916 Brugia malayi|Elephantiasis roundworm|CDQ04279 Steinernema carpocapsae|Entomopathogenic roundworm|TKR89621 100 Steinernema carpocapsae|Entomopathogenic roundworm|TKR93888 Strongyloides ratti|Rat roundworm|XP 024507084 Strongyloides ratti|Rat roundworm|XP 024509240 100 Strongyloides ratti|Rat roundworm|XP 024509050 Pristionchus pacificus|Nematode|PDM75839 Diploscapter pachys|Nematode|PAV63198 Caenorhabditis elegans|Nematode|gon-2 CE30390 Caenorhabditis remanei|Nematode|XP 003112078 100 100 Caenorhabditis remanei|Nematode|OZF79912
99 Caenorhabditis brenneri|Nematode|EGT32766 Caenorhabditis nigoni|Nematode|PIC52068 95 100 Caenorhabditis briggsae|Nematode|CAP31489.2
Duplications before Cniadaria-Bilateria split Figure S10 100 TRPS Praesagittifera naikaiensis|Acoel flatworm|g40739.t1 Aurelia|Moon jelly|Seg1803.3 transcript id Seg1803.3|GoldUCD|mRNA.D3Y31 Hofstenia miamia|Three-banded panther worm|scaffold112 437721 469571 R
100 Hofstenia miamia|Three-banded panther worm|scaffold18 807971 810976 F 100 100 Hofstenia miamia|Three-banded panther worm|scaffold18 866412 869501 F Praesagittifera naikaiensis|Acoel flatworm|g16021.t1 100 100 Hofstenia miamia|Three-banded panther worm|scaffold488 5372 8923 F Praesagittifera naikaiensis|Acoel flatworm|g4935.t1 100 Praesagittifera naikaiensis|Acoel flatworm|g36760.t1 95 Praesagittifera naikaiensis|Acoel flatworm|g11490.t1 Hofstenia miamia|Three-banded panther worm|scaffold387 196851 200279 F Hofstenia miamia|Three-banded panther worm|scaffold387 254022 257456 F ? 100 100 Hofstenia miamia|Three-banded panther worm|scaffold387 247539 251000 F 100 99 Hofstenia miamia|Three-banded panther worm|scaffold387 219858 223314 F
100 Hofstenia miamia|Three-banded panther worm|scaffold391 331241 359300 R 100 Hofstenia miamia|Three-banded panther worm|scaffold387 211324 251000 F 100 Pocillopora damicornis|Lace coral|pdam 00007420-RA 100 Stylophora pistillata|Hood coral|Spis14035.t1 SpisGene14035 100 Fungia spp.|Coral|ffun1.m4.21870.m1 Galaxea fascicularis|Galaxy coral|gfas1.m1.11287.m1 Goniastrea aspera|Stony coral|gasp1.m3.6245.m1 Limulus polyphemus|American horseshoe crab|XP 022255565 100
100 Parasteatoda tepidariorum|American house spider|XP 015907052
100 Stegodyphus mimosarum|African social velvet spider|KFM65832 100 Araneus ventricosus|Orb-weaver spider|GBM10676 100 Tigriopus californicus|Copepod|TRY76337
100 Eurytemora affinis|Copepod|XP 023322531 100 Tigriopus californicus|Copepod|TRY77421 100 Amphibalanus improvisus|Bay barnacle|QCO69752
100 Hyalella azteca|Freshwater amphipod|XP 018027820
100 Penaeus vannamei|Whiteleg shrimp|ROT84591
100 Penaeus vannamei|Whiteleg shrimp|XP 027222949 100 Penaeus vannamei|Whiteleg shrimp|ROT85499 Praesagittifera naikaiensis|Acoel flatworm|g14495.t1
98 Praesagittifera naikaiensis|Acoel flatworm|g14113.t1 100 Praesagittifera naikaiensis|Acoel flatworm|g10675.t1 Priapulus caudatus|Penis worm|XP 014669422
98 Homo sapiens|Human|TRPM8 CCDS33407.1 98 100 Homo sapiens|Human|TRPM2 CCDS13710.1 100 Homo sapiens|Human|TRPM5 CCDS31340.1 100 Homo sapiens|Human|TRPM4 CCDS33073.1 99 Hydra vulgaris|Fresh-water polyp|XP 012565257 95 Aurelia|Moon jelly|Seg1831.8 transcript id Seg1831.8|GoldUCD|mRNA.D3Y31 Nematostella vectensis|Starlet sea anemone|Nemve1|248535 100 Pocillopora damicornis|Lace coral|pdam 00009856-RA β 100 100 Stylophora pistillata|Hood coral|Spis4675 SpisGene4675 Fungia spp.|Coral|ffun1.m4.19256.m1 100 100 Goniastrea aspera|Stony coral|gasp1.m3.30302.m1
99 Porites lutea|Small polyp stony coral|plut2.m8.2561.m1
100 Galaxea fascicularis|Galaxy coral|gfas1.m1.6599.m1
99 Acropora digitifera|Coral|adi v1.10270 100 Acropora tenuis|Coral|aten 0.1.m1.1851.m1 Hydra vulgaris|Fresh-water polyp|XP 012554388
100 Hydra vulgaris|Fresh-water polyp|XP 012562662 100 Hydra vulgaris|Fresh-water polyp|XP 012561335 Aiptasia|Sea anemone|AIPGENE12924
98 Nematostella vectensis|Starlet sea anemone|Nemve1|85952 Pocillopora damicornis|Lace coral|pdam 00014050-RA
100 Goniastrea aspera|Stony coral|gasp1.m3.26670.m1 100 Fungia spp.|Coral|ffun1.m4.18855.m1 100 Aiptasia|Sea anemone|AIPGENE19094 100 Nematostella vectensis|Starlet sea anemone|Nemve1|102167 99 Porites lutea|Small polyp stony coral|plut2.m8.21682.m1 α 99 100 Pocillopora damicornis|Lace coral|pdam 00020220-RA
98 Goniastrea aspera|Stony coral|gasp1.m3.6037.m1 100 Fungia spp.|Coral|ffun1.m4.18290.m1 Praesagittifera naikaiensis|Acoel flatworm|g25700.t1 Homo sapiens|Human|TRPM3 CCDS6634.1 100 Homo sapiens|Human|TRPM1 CCDS10024.2 100 Homo sapiens|Human|TRPM7 CCDS42035.1 100 100 Homo sapiens|Human|TRPM6 CCDS6647.1 Priapulus caudatus|Penis worm|XP 014671784 Limulus polyphemus|American horseshoe crab|XP 022248148 100 100 Centruroides sculpturatus|Arizona bark scorpion|XP 023237252 Ixodes scapularis|Deer tick|XP 029824440 Daphnia magna|Water flea|KZS13223
100 Daphnia pulex|Water flea|EFX80451 100 100 Daphnia magna|Water flea|KZS13280 Armadillidium vulgare|Roly-poly|RXG60922 Penaeus vannamei|Whiteleg shrimp|XP 027220225 100 100 Penaeus vannamei|Whiteleg shrimp|XP 027220241 100 Cancer borealis|Jonah crab|APG53781
100 100 Homarus americanus|American lobster|APG53788 Orchesella cincta|Belted springtail|ODM97521 Neodiprion lecontei|Redhead pine sawfly|XP 015512476 100 Neodiprion lecontei|Redhead pine sawfly|XP 015512479 Orussus abietinus|Parasitic wood wasp|XP 012280557 100 Orussus abietinus|Parasitic wood wasp|XP 012280565 Cephus cinctus|Wheat stem sawfly|XP 015594698
100 Cephus cinctus|What stem sawfly|XP 015594691
100 Cephus cinctus|Wheat stem sawfly|XP 015594697 100 Diachasma alloeum|Parisitoid wasp|XP 015112330 100 Fopius arisanus|Braconid wasp|XP 011307041 100 Microplitis demolitor|Parasitoid wasp|XP 014296021 100 Microplitis demolitor|Parasitoid wasp|XP 008545401 Trichogramma pretiosum|Polyphagous wasp|XP 014228063 100 100 Trichogramma pretiosum|Polyphagous wasp|XP 014228070
100 Copidosoma floridanum|Parasitoid wasp|XP 023245943
99 Ceratosolen solmsi marchali|Fig wasp|XP 011494034
100 Nasonia vitripennis|Parasitoid wasp|XP 016845126 100 Trichomalopsis sarcophagae|Wasp|OXU23397 Ceratina calcarata|Spurred ceratina bee|XP 026670292 100 Ceratina calcarata|Spurred ceratina bee|XP 026670488 100 Megachile rotundata|Alfalfa leafcutting bee|XP 012140911 100 100 Osmia bicornis bicornis|Red mason bee|XP 029053463
99 Habropoda laboriosa|Blueberry digger bee|KOC68432 Eufriesea mexicana|Mexican orchid bee|XP 017763457 100 Melipona quadrifasciata|Mandaaia bee|KOX68915
100 100 Bombus impatiens|Common eastern bumblebee|XP 012238938 100 Bombus terrestris|Buff-tailed bumblebee|XP 012166409
100 Apis cerana cerana|Eastern honey bee|XP 028521981 100 100 Apis cerana cerana|Eastern honey bee|PBC31881 100 Apis florea|Red dwarf honey bee|XP 003695376
99 Apis mellifera|European honey bee|XP 016769201 99 Apis dorsata|Giant honey bee|XP 006617666 Polistes dominula|European paper wasp|XP 015190771 100 Polistes canadensis|Red paper wasp|XP 014600553 Harpegnathos saltator|Jerdon s jumping ant|XP 019700199.2 100 Dinoponera quadriceps|South American ant|XP 014480910 Linepithema humile|Argentine ant|XP 012234779
100 99 Pseudomyrmex gracilis|Slender twig ant|XP 020300482 100 Pseudomyrmex gracilis|Slender twig ant|XP 020300486 Ooceraea biroi|Clonal raider ant|EZA58689 99 100 Ooceraea biroi|Clonal raider ant|XP 011331801 Nylanderia fulva|Tawny crazy ant|XP 029159541 100 97 100 Formica exsecta|Narrow-headed ant|XP 029678003 100 Camponotus floridanus|Florida carpenter ant|XP 025271119
97 Pogonomyrmex barbatus|Red harvester ant|XP 025073334 Temnothorax curvispinosus|Ant|XP 024882761 100 100 Temnothorax longispinosus|Ant|TGZ50551 Monomorium pharaonis|Pharaoh ant|XP 012538403 98 Vollenhovia emeryi|Ant|XP 011879164
97 Wasmannia auropunctata|Little fire ant|XP 011703736 Cyphomyrmex costatus|Fungus growing ant|KYN00283 98 Trachymyrmex zeteki|Fungus-growing ant|KYQ46528 100 100 Trachymyrmex zeteki|Fungus growing ant|XP 018316097
100 Trachymyrmex septentrionalis|Leaf-cutter ant|XP 018348579
100 Trachymyrmex cornetzi|Fungus-growing ant|XP 018375041
100 Acromyrmex echinatior|Leaf-cutting ant|XP 011055821
100 Atta colombica|Leaf-cutter ant|XP 018049373 100 Atta cephalotes|Leaf-cutter ant|XP 012064138 Blattella germanica|German cockroach|PSN39268
100 Zootermopsis nevadensis|Termite|XP 021919466
99 Cryptotermes secundus|West Indian drywood termite|XP 023715316 100 Cryptotermes secundus|West Indian drywood termite|XP 023710445 100 Frankliniella occidentalis|Western flower thrips|XP 026274853 Pediculus humanus corporis|Body louse|XP 002423257 Laodelphax striatellus|Small brown planthopper|RZF33234 100 Nilaparvata lugens|Brown planthopper|XP 022186364
98 Bemisia tabaci|Silverleaf whitefly|XP 018904967 Sipha flava|Yellow sugarcane aphid|XP 025425746 97 Rhopalosiphum maidis|Corn leaf aphid|XP 026809557 100 Aphis gossypii|Melon and cotton aphid|XP 027836600 Melanaphis sacchari|Sugarcane aphid|XP 025203880 100 Diuraphis noxia|Russian wheat aphid|XP 015367699
95 Myzus persicae|Green peach aphid|XP 022180245 Acyrthosiphon pisum|Pea aphid|XP 003245193 Agrilus planipennis|Emerald ash borer|XP 025833357 Nicrophorus vespilloides|Buring beetle|XP 017773716
96 100 Nicrophorus vespilloides|Boreal carrion beetle|XP 017773713 Nicrophorus vespilloides|Boreal carrion beetle|XP 017773706 Nicrophorus vespilloides|Boreal carrion beetle|XP 017773711 100 Onthophagus taurus|Taurus scarab|XP 022904186 100 Onthophagus taurus|Taurus scarab|XP 022904266 Asbolus verrucosus|Desert ironclad beetle|RZC40720
100 Tribolium castaneum|Red flour beetle|XP 015837221
100 Tribolium castaneum|Red flour beetle|XP 015837222 Tribolium castaneum|Red flour beetle|XP 015837215 100 Aethina tumida|Small hive beetle|XP 019874384 Dendroctonus ponderosae|Mountain pine beetle|ENN80022 100 100 Dendroctonus ponderosae|Mountain pine beetle|XP 019755236 100 Anoplophora glabripennis|Asian longhorned beetle|XP 023311041
100 Diabrotica virgifera virgifera|Western corn rootworm|XP 028136863 100 Leptinotarsa decemlineata|Colorado potato beetle|XP 023017091
100 Ctenocephalides felis|Cat flea|XP 026479982 Clunio marinus|Midge|CRL00240 Anopheles darlingi|Mosquito|ETN57805
100 100 Anopheles gambiae senso stricto|Mosquito|XP 308919.3
100 100 Anopheles sinensis|Mosquito|KFB51525 100 Aedes aegypti|Yellow fever mosquito|XP 021698933
100 Aedes albopictus|Asian tiger mosquito|XP 029735731
99 Aedes albopictus|Asian tiger mosquito|XP 029734607 100 98 Aedes albopictus|Asian tiger mosquito|KXJ83914 Drosophila melanogaster|Common fruit fly|Trpm
100 Lucilia cuprina|Australian sheep blowflow|XP 023291853
100 Stomoxys calcitrans|Stable fly|XP 013109884
100 100 Musca domestica|Housefly|XP 011296059 100 Bactrocera latifrons|Solanum fruit fly|XP 018803596
100 Zeugodacus cucurbitae|Melon fly|XP 011178713
100 Bactrocera dorsalis|Oriental fruit fly|XP 019844435
100 Ceratitis capitata|Mediterranean fruit fly|XP 012154700 100 Bactrocera oleae|Olive fruit fly|XP 014099935 Hyposmocoma kahamanoa|Moth|XP 026320800 Manduca sexta|Tobacco hornworm Carolina sphinx moth|QDR51034 100 Manduca sexta|Tobacco hornworm Carolina sphinx moth|XP 030030234
100 100 Bombyx mandarina|Wild silkmoth|XP 028025209 100 Bombyx mori|Domestic silkmoth|XP 012551960 Trichoplusia ni|Cabbage looper moth|XP 026740408
99 Spodoptera litura|Tobacco cutworm moth|XP 022827232
99 Helicoverpa armigera|Cotton bollworm moth|PZC87367 100 Helicoverpa armigera|Cottom bollworm moth|XP 021181239 Chilo suppressalis|Asiatic rice borer moth|RVE51394 Galleria mellonella|Great wax moth|XP 026762983 100 Amyelois transitella|Navel orangeworm snout moth|XP 013187551 Danaus plexippus|Monarch butterfly|QDQ16920 Bicyclus anynana|Squinting bush brown butterfly|XP 023939913 Vanessa tameamea|Kamehameha butterfly|XP 026491684 Heliconius melpomene|Postman butterfly|QDR50959 Pieris rapae|Small cabbage white butterfly|XP 022128471 Papilio polytes|Common Mormon butterfly|XP 013137248
100 Papilio xuthus|Asian swallowtail butterfly|KPJ04070
100 Papilio xuthus|Asian swallowtail butterfly|XP 013162535 Duplications before 95 Papilio machaon|Old World swallowtail butterfly|KPJ09541 99 Papilio machaon|Old World swallowtail butterfly|XP 014365775 Cniadaria-Bilateria split Figure S11 100 TRPS
Aurelia|Moon jelly|Seg1803.3 transcript id Seg1803.3|GoldUCD|mRNA.D3Y31 Pocillopora damicornis|Lace coral|pdam 00007420-RA 100 Stylophora pistillata|Hood coral|Spis14035.t1 SpisGene14035 100 Fungia spp.|Coral|ffun1.m4.21870.m1 β 95 Goniastrea aspera|Stony coral|gasp1.m3.6245.m1 96 Galaxea fascicularis|Galaxy coral|gfas1.m1.11287.m1 Homo sapiens|Human|TRPM8 CCDS33407.1 100 Homo sapiens|Human|TRPM2 CCDS13710.1 100 Homo sapiens|Human|TRPM5 CCDS31340.1 100 Homo sapiens|Human|TRPM4 CCDS33073.1 Acanthaster planci|Crown-of-thorns starfish|XP 022086910.1 Ptychodera flava|Acorn worm|pfl 40v0 9 20150316 1g24145. Saccoglossus kowalevskii|Acorn worm|XP 006816420 98 Acanthaster planci|Crown-of-thorns starfish|XP 022096576.1 100 Strongylocentrotus purpuratus|Purple sea urchin|XP 011661004.1 Ptychodera flava|Acorn worm|pfl 40v0 9 20150316 1g5514. Ptychodera flava|Acorn worm|pfl 40v0 9 20150316 1g7524. 97 Ptychodera flava|Acorn worm|pfl 40v0 9 20150316 1g8370. 100 Ptychodera flava|Acorn worm|pfl 40v0 9 20150316 1g8628. 98 Ptychodera flava|Acorn worm|pfl 40v0 9 20150316 1g26243. Strongylocentrotus purpuratus|Purple sea urchin|XP 011664673.1 Apostichopus japonicus|Japanese sea cucumber|PIK58237.1 100 100 Acanthaster planci|Crown-of-thorns starfish|XP 022110675.1 100 100 Acanthaster planci|Crown-of-thorns starfish|XP 022110674.1 100 Strongylocentrotus purpuratus|Purple sea urchin|XP 011674872.1 Apostichopus japonicus|Japanese sea cucumber|PIK46475.1 100 Acanthaster planci|Crown-of-thorns starfish|XP 022096929.1 100 Acanthaster planci|Crown-of-thorns starfish|XP 022084590.1 100 Acanthaster planci|Crown-of-thorns starfish|XP 022084599.1 Saccoglossus kowalevskii|Acorn worm|XP 006814003 100 Strongylocentrotus purpuratus|Purple sea urchin|XP 794425.3 100 Acanthaster planci|Crown-of-thorns starfish|XP 022099673.1 100 Drosophila melanogaster|Common fruit fly|Trpm α Homo sapiens|Human|TRPM3 CCDS6634.1 99 100 Homo sapiens|Human|TRPM1 CCDS10024.2 100 Homo sapiens|Human|TRPM7 CCDS42035.1 100 Homo sapiens|Human|TRPM6 CCDS6647.1 Hydra vulgaris|Fresh-water polyp|XP 012565257 Aurelia|Moon jelly|Seg1831.8 transcript id Seg1831.8|GoldUCD|mRNA.D3Y31 Nematostella vectensis|Starlet sea anemone|Nemve1|248535 100 Porites lutea|Small polyp stony coral|plut2.m8.2561.m1 Galaxea fascicularis|Galaxy coral|gfas1.m1.6599.m1 100 99 100 Acropora digitifera|Coral|adi v1.10270 100 Acropora tenuis|Coral|aten 0.1.m1.1851.m1 100 Fungia spp.|Coral|ffun1.m4.19256.m1 Goniastrea aspera|Stony coral|gasp1.m3.30302.m1 Pocillopora damicornis|Lace coral|pdam 00009856-RA 100 Stylophora pistillata|Hood coral|Spis4675 SpisGene4675 Hydra vulgaris|Fresh-water polyp|XP 012554388 100 Hydra vulgaris|Fresh-water polyp|XP 012562662 100 Hydra vulgaris|Fresh-water polyp|XP 012561335 Aiptasia|Sea anemone|AIPGENE12924 99 Nematostella vectensis|Starlet sea anemone|Nemve1|85952 98 97 Pocillopora damicornis|Lace coral|pdam 00014050-RA 100 Goniastrea aspera|Stony coral|gasp1.m3.26670.m1 99 Fungia spp.|Coral|ffun1.m4.18855.m1 100 Aiptasia|Sea anemone|AIPGENE19094 100 Nematostella vectensis|Starlet sea anemone|Nemve1|102167 Porites lutea|Small polyp stony coral|plut2.m8.21682.m1 Pocillopora damicornis|Lace coral|pdam 00020220-RA 100 100 Goniastrea aspera|Stony coral|gasp1.m3.6037.m1 100 Fungia spp.|Coral|ffun1.m4.18290.m1
Duplications before Cniadaria-Bilateria split
Figure S12 100 TRPS Hydra vulgaris|Fresh-water polyp|XP 012565257 Aurelia|Moon jelly|Seg1831.8 transcript id Seg1831.8|GoldUCD|mRNA.D3Y31 Nematostella vectensis|Starlet sea anemone|Nemve1|248535 100 Pocillopora damicornis|Lace coral|pdam 00009856-RA 100 100 Stylophora pistillata|Hood coral|Spis4675 SpisGene4675 α Fungia spp.|Coral|ffun1.m4.19256.m1 100 99 Goniastrea aspera|Stony coral|gasp1.m3.30302.m1 98 Porites lutea|Small polyp stony coral|plut2.m8.2561.m1 Galaxea fascicularis|Galaxy coral|gfas1.m1.6599.m1 100 100 Acropora digitifera|Coral|adi v1.10270 100 Acropora tenuis|Coral|aten 0.1.m1.1851.m1 Drosophila melanogaster|Common fruit fly|Trpm Ciona intestinalis|Vase tunicate|XP 018667443 Branchiostoma lanceolatum|European lacelet|BL24111 evm0 100 100 Branchiostoma belcheri|Lancelet|XP 019640450 Chiloscyllium punctatum|Brownbanded bamboo shark|Chipu0016510.p1 TRPM3 100 Callorhinchus milii|Ghostshark|XP 007904772.1 100 Latimeria chalumnae|Coelacanth|XP 014347481.1 Gallus gallus|Chicken|TRPM3 F1NJZ7 CHICK 100 100 Mus musculus|Mouse|Trpm3 CCDS29702.1 Homo sapiens|Human|TRPM3 CCDS6634.1 100 100 100 Eptatretus burgeri|Inshore hagfish|trpm1b-201|ENSEBUP00000012388 Latimeria chalumnae|Coelacanth|XP 014348116.1 Gallus gallus|Chicken|TRPM1 F1NCK1 CHICK 100 100 Mus musculus|Mouse|TRPM1 CCDS21332.2 TRPM1 100 100 Homo sapiens|Human|TRPM1 CCDS10024.2 100 Callorhinchus milii|Ghostshark|XP 007907039.1 Rhincodon typus|Whale shark|XP 020377793.1 100 100 100 Carcharodon carcharias|Great white shark|ScRDnwc 41824 761978-snap-gene-2.12-mRNA-1 100 Chiloscyllium punctatum|Brownbanded bamboo shark|Chipu0014745.pl Callorhinchus milii|Ghostshark|XP 007897564.1 100 Scyliorhinus torazame|Cloudy catshark|Scyto0003223.p1 TRPM6 100 Chiloscyllium punctatum|Brownbanded bamboo shark|Chipu0003513.p1 100 Latimeria chalumnae|Coelacanth|XP 014349563.1 Gallus gallus|Chicken|TRPM6 E1C5L2 CHICK 99 99 Mus musculus|Mouse|Trpm6 CCDS29691.1 100 100 100 Homo sapiens|Human|TRPM6 CCDS6647.1 Petromyzon marinus|Sea lamprey|PMZ 0026066-RA Latimeria chalumnae|Coelacanth|XP 005989708.1 Gallus gallus|Chicken|TRPM7 E1BYV3 CHICK 99 100 Mus musculus|Mouse|Trpm7 CCDS16689.1 TRPM7 100 100 Homo sapiens|Human|TRPM7 CCDS42035.1 100 Callorhinchus milii|Ghostshark|XP 007903830.1 Chiloscyllium punctatum|Brownbanded bamboo shark|Chipu0017399.p1 100 100 Rhincodon typus|Whale shark|XP 020370945.1 100 Carcharodon carcharias|Great white shark|ScRDnwc 11009 1186063-snap-gene-4.2-mRNA-1 100 Scyliorhinus torazame|Cloudy catshark|Scyto0018498.p1 Aurelia|Moon jelly|Seg1803.3 transcript id Seg1803.3|GoldUCD|mRNA.D3Y31 Hydra vulgaris|Fresh-water polyp|XP 012554388 100 Hydra vulgaris|Fresh-water polyp|XP 012562662 100 Hydra vulgaris|Fresh-water polyp|XP 012561335 Pocillopora damicornis|Lace coral|pdam 00007420-RA 98 Stylophora pistillata|Hood coral|Spis14035.t1 SpisGene14035 β 100 Fungia spp.|Coral|ffun1.m4.21870.m1 Galaxea fascicularis|Galaxy coral|gfas1.m1.11287.m1 Goniastrea aspera|Stony coral|gasp1.m3.6245.m1 Aiptasia|Sea anemone|AIPGENE12924 Nematostella vectensis|Starlet sea anemone|Nemve1|85952 Pocillopora damicornis|Lace coral|pdam 00014050-RA 100 Goniastrea aspera|Stony coral|gasp1.m3.26670.m1 99 Fungia spp.|Coral|ffun1.m4.18855.m1 100 Aiptasia|Sea anemone|AIPGENE19094 100 Nematostella vectensis|Starlet sea anemone|Nemve1|102167 100 Porites lutea|Small polyp stony coral|plut2.m8.21682.m1 Pocillopora damicornis|Lace coral|pdam 00020220-RA 100 100 Goniastrea aspera|Stony coral|gasp1.m3.6037.m1 100 Fungia spp.|Coral|ffun1.m4.18290.m1 Ciona intestinalis|Vase tunicate|XP 026692588 Oikopleura dioica|Tunicate|CBY19337 Oikopleura dioica|Tunicate|CBY13016 100 Oikopleura dioica|Tunicate|CBY10098 Branchiostoma floridae|Florida lancelet|XP 002592165 Branchiostoma belcheri|Lancelet|XP 019638014 Branchiostoma floridae|Florida lancelet|XP 002605080 99 100 Branchiostoma floridae|Florida lancelet|XP 002592248 100 Branchiostoma belcheri|Lancelet|XP 019640471 TRPM8 Latimeria chalumnae|Coelacanth|XP 014345846.1 Gallus gallus|Chicken|TRPM8 E1BWL7 CHICK 100 100 Mus musculus|Mouse|Trpm8 CCDS48316.1 100 Homo sapiens|Human|TRPM8 CCDS33407.1 Latimeria chalumnae|Coelacanth|XP 014345762.1 100 Gallus gallus|Chicken|TRPM2 F1NGK6 CHICK 100 100 Mus musculus|Mouse|Trpm2 CCDS48611.1 100 Homo sapiens|Human|TRPM2 CCDS13710.1 100 Callorhinchus milii|Ghostshark|XP 007888802.1 TRPM2 100 Callorhinchus milii|Ghostshark|XP 007888801.1 100 Scyliorhinus torazame|Cloudy catshark|Scyto0014884.p1 100 100 Chiloscyllium punctatum|Brownbanded bamboo shark|Chipu0018262.p1 100 Rhincodon typus|Whale shark|XP 020374811.1 Mus musculus|Mouse|Trpm4 CCDS52245.1 TRPM4 100 Homo sapiens|Human|TRPM4 CCDS33073.1 99 Scyliorhinus torazame|Cloudy catshark|Scyto0007372.p1 100 Latimeria chalumnae|Coelacanth|XP 005990988.1 Latimeria chalumnae|Coelacanth|XP 014344856.1 100 Gallus gallus|Chicken|TRPM5 E1BXA4 CHICK 100 100 Mus musculus|Mouse|Trpm5 CCDS52462.1 100 Homo sapiens|Human|TRPM5 CCDS31340.1 100 Callorhinchus milii|Ghostshark|XP 007902997.1 TRPM5 Scyliorhinus torazame|Cloudy catshark|Scyto0000828.p1 99 Carcharodon carcharias|Great white shark|ScRDnwc 40374 6376172-snap-gene-36.2-mRNA-1 100 Chiloscyllium punctatum|Brownbanded bamboo shark|Chipu0004516.p1 Rhincodon typus|Whale shark|XP 020389255.1 Duplications before Cniadaria-Bilateria split Figure S13 100 TRPS Hydra vulgaris|Fresh-water polyp|XP 012554388 100 Hydra vulgaris|Fresh-water polyp|XP 012562662 100 Hydra vulgaris|Fresh-water polyp|XP 012561335 Aiptasia|Sea anemone|AIPGENE12924 Nematostella vectensis|Starlet sea anemone|Nemve1|85952 Pocillopora damicornis|Lace coral|pdam 00014050-RA α 100 Goniastrea aspera|Stony coral|gasp1.m3.26670.m1 99 Fungia spp.|Coral|ffun1.m4.18855.m1 99 Aiptasia|Sea anemone|AIPGENE19094 100 Nematostella vectensis|Starlet sea anemone|Nemve1|102167 99 Pocillopora damicornis|Lace coral|pdam 00020220-RA Porites lutea|Small polyp stony coral|plut2.m8.21682.m1 95 100 Goniastrea aspera|Stony coral|gasp1.m3.6037.m1 100 Fungia spp.|Coral|ffun1.m4.18290.m1 Homo sapiens|Human|TRPM3 CCDS6634.1 100 Homo sapiens|Human|TRPM1 CCDS10024.2 100 Homo sapiens|Human|TRPM7 CCDS42035.1 100 Homo sapiens|Human|TRPM6 CCDS6647.1 100 Drosophila melanogaster|Common fruit fly|Trpm Helobdella robusta|Leech|XP 009013900.1 100 Helobdella robusta|Leech|XP 009015939.1 100 Helobdella robusta|Leech|XP 009009645.1 Notospermus geniculatus|Ribbon worm|g23156.t1 Lingula anatina|Brachiopod|XP 013397522.1 Phoronis australis|Horseshoe worm|g14558.t1 Octopus vulgaris|Common octopus|XP 029641654.1 100 Octopus bimaculoides|California two-spot octopus|XP 014787598.1 98 Aplysia californica|California sea hare|XP 012936450.1 100 Biomphalaria glabrata|Freshwater snail|XP 013083453.1 100 Biomphalaria glabrata|Freshwater snail|XP 013083410.1 100 Mizuhopecten yessoensis|Yesso scallop|XP 021372912.1 98 Mizuhopecten yessoensis|Yesso scallop|OWF43174.1 100 Mizuhopecten yessoensis|Yesso scallop|XP 021368426.1 100 100 Crassostrea gigas|Pacific oyster|XP 011435194.1 100 Crassostrea gigas|Pacific oyster|XP 011435204.1 100 Crassostrea gigas|Pacific oyster|XP 011414379.1 100 Crassostrea virginica|Eastern oyster|XP 022295841.1 Aurelia|Moon jelly|Seg1803.3 transcript id Seg1803.3|GoldUCD|mRNA.D3Y31 Pocillopora damicornis|Lace coral|pdam 00007420-RA 100 Stylophora pistillata|Hood coral|Spis14035.t1 SpisGene14035 100 Fungia spp.|Coral|ffun1.m4.21870.m1 Galaxea fascicularis|Galaxy coral|gfas1.m1.11287.m1 Goniastrea aspera|Stony coral|gasp1.m3.6245.m1 Hydra vulgaris|Fresh-water polyp|XP 012565257 β 99 Aurelia|Moon jelly|Seg1831.8 transcript id Seg1831.8|GoldUCD|mRNA.D3Y31 Nematostella vectensis|Starlet sea anemone|Nemve1|248535 100 Pocillopora damicornis|Lace coral|pdam 00009856-RA 100 Stylophora pistillata|Hood coral|Spis4675 SpisGene4675 100 100 Fungia spp.|Coral|ffun1.m4.19256.m1 99 Goniastrea aspera|Stony coral|gasp1.m3.30302.m1 100 Porites lutea|Small polyp stony coral|plut2.m8.2561.m1 Galaxea fascicularis|Galaxy coral|gfas1.m1.6599.m1 100 100 Acropora digitifera|Coral|adi v1.10270 100 Acropora tenuis|Coral|aten 0.1.m1.1851.m1 Homo sapiens|Human|TRPM8 CCDS33407.1 100 Homo sapiens|Human|TRPM2 CCDS13710.1 100 Homo sapiens|Human|TRPM5 CCDS31340.1 100 Homo sapiens|Human|TRPM4 CCDS33073.1 Lingula anatina|Brachiopod|XP 023931219.1 100 Capitella teleta|Annelid worm|ELT87598.1 Lingula anatina|Brachiopod|XP 013398789.1 Phoronis australis|Horseshoe worm|g2633.t1 99 99 Helobdella robusta|Leech|XP 009013475.1 98 Capitella teleta|Annelid worm|ELU00429.1 Capitella teleta|Annelid worm|ELT91987.1 100 Capitella teleta|Annelid worm|ELT97859.1 100 Capitella teleta|Annelid worm|ELT96530.1 Helobdella robusta|Leech|XP 009025643.1 100 Capitella teleta|Annelid worm|ELT97299.1 Notospermus geniculatus|Ribbon worm|g1465.t1 Notospermus geniculatus|Ribbon worm|g14995.t1 100 100 100 Notospermus geniculatus|Ribbon worm|g29200.t1 Lingula anatina|Brachiopod|XP 013384361.1 Phoronis australis|Horseshoe worm|g10339.t1 Mizuhopecten yessoensis|Yesso scallop|OWF37798.1 Crassostrea virginica|Eastern oyster|XP 022319512.1 100 100 Crassostrea gigas|Pacific oyster|XP 011414360.1 100 Crassostrea gigas|Pacific oyster|XP 011456090.1 100 Pomacea canaliculata|Golden apple snail|PVD39019.1 100 Pomacea canaliculata|Golden apple snail|XP 025089091.1 100 Biomphalaria glabrata|Freshwater snail|XP 013061400.1 100 Aplysia californica|California sea hare|XP 005091977.2 100 Biomphalaria glabrata|Freshwater snail|XP 013061392.1 Octopus vulgaris|Common octopus|XP 029647149.1 Octopus vulgaris|Common octopus|XP 029646813.1 100 100 Octopus bimaculoides|California two-spot octopus|XP 014781149.1 100 Octopus vulgaris|Common octopus|XP 029646968.1 Mizuhopecten yessoensis|Yesso scallop|XP 021352500.1 Crassostrea virginica|Eastern oyster|XP 022338390.1 100 Crassostrea virginica|Eastern oyster|XP 022339354.1 Pomacea canaliculata|Golden apple snail|PVD30001.1 100 Aplysia californica|California sea hare|XP 005094347.1 100 Biomphalaria glabrata|Freshwater snail|XP 013090385.1 Lottia gigantea|Owl limpet|XP 009055754.1 Pomacea canaliculata|Golden apple snail|PVD35972.1 100 100 Pomacea canaliculata|Golden apple snail|XP 025081224.1 Aplysia californica|California sea hare|XP 012942949.1 98 100 Biomphalaria glabrata|Freshwater snail|XP 013063620.1 99 Aplysia californica|California sea hare|XP 005100068.2 100 Aplysia californica|California sea hare|XP 012940727.1 Crassostrea gigas|Pacific oyster|XP 011446980.2 100 Crassostrea virginica|Eastern oyster|XP 022342892.1 Mizuhopecten yessoensis|Yesso scallop|XP 021375672.1 100 Mizuhopecten yessoensis|Yesso scallop|XP 021375666.1 Mizuhopecten yessoensis|Yesso scallop|XP 021378397.1 100 Crassostrea gigas|Pacific oyster|XP 019919132.1 100 Crassostrea virginica|Eastern oyster|XP 022340640.1 Mizuhopecten yessoensis|Yesso scallop|XP 021362498.1 Crassostrea virginica|Eastern oyster|XP 022336960.1 96 Crassostrea gigas|Pacific oyster|XP 019924531.1 100 Crassostrea gigas|Pacific oyster|XP 011433270.1 Duplications before Crassostrea gigas|Pacific oyster|XP 019924535.1 Cniadaria-Bilateria split Figure S14 100 TRPS Aurelia|Moon jelly|Seg1803.3 transcript id Seg1803.3|GoldUCD|mRNA.D3Y31 Fungia spp.|Coral|ffun1.m4.21870.m1 Galaxea fascicularis|Galaxy coral|gfas1.m1.11287.m1 100 Goniastrea aspera|Stony coral|gasp1.m3.6245.m1 100 Pocillopora damicornis|Lace coral|pdam 00007420-RA 100 Stylophora pistillata|Hood coral|Spis14035.t1 SpisGene14035 Priapulus caudatus|Penis worm|XP 014669422 Toxocara canis|Dog roundworm|VDM40999 β 100 Toxocara canis|Dog roundworm|KHN83770 Homo sapiens|Human|TRPM8 CCDS33407.1 100 Homo sapiens|Human|TRPM2 CCDS13710.1 100 Homo sapiens|Human|TRPM5 CCDS31340.1 100 Homo sapiens|Human|TRPM4 CCDS33073.1 Hydra vulgaris|Fresh-water polyp|XP 012565257 Aurelia|Moon jelly|Seg1831.8 transcript id Seg1831.8|GoldUCD|mRNA.D3Y31 Nematostella vectensis|Starlet sea anemone|Nemve1|248535 100 Porites lutea|Small polyp stony coral|plut2.m8.2561.m1 100 100 Galaxea fascicularis|Galaxy coral|gfas1.m1.6599.m1 Acropora digitifera|Coral|adi v1.10270 100 100 99 Acropora tenuis|Coral|aten 0.1.m1.1851.m1 Fungia spp.|Coral|ffun1.m4.19256.m1 98 α Goniastrea aspera|Stony coral|gasp1.m3.30302.m1 100 Pocillopora damicornis|Lace coral|pdam 00009856-RA 100 Stylophora pistillata|Hood coral|Spis4675 SpisGene4675 Hydra vulgaris|Fresh-water polyp|XP 012554388 100 Hydra vulgaris|Fresh-water polyp|XP 012562662 100 Hydra vulgaris|Fresh-water polyp|XP 012561335 Aiptasia|Sea anemone|AIPGENE12924 Nematostella vectensis|Starlet sea anemone|Nemve1|85952 96 Pocillopora damicornis|Lace coral|pdam 00014050-RA 100 Fungia spp.|Coral|ffun1.m4.18855.m1 Goniastrea aspera|Stony coral|gasp1.m3.26670.m1 97 Aiptasia|Sea anemone|AIPGENE19094 100 Nematostella vectensis|Starlet sea anemone|Nemve1|102167 Porites lutea|Small polyp stony coral|plut2.m8.21682.m1 Pocillopora damicornis|Lace coral|pdam 00020220-RA 100 100 Goniastrea aspera|Stony coral|gasp1.m3.6037.m1 100 Fungia spp.|Coral|ffun1.m4.18290.m1 Homo sapiens|Human|TRPM3 CCDS6634.1 100 Homo sapiens|Human|TRPM1 CCDS10024.2 100 Homo sapiens|Human|TRPM7 CCDS42035.1 100 Homo sapiens|Human|TRPM6 CCDS6647.1 Priapulus caudatus|Penis worm|XP 014671784 Drosophila melanogaster|Common fruit fly|Trpm 95 Trichuris trichiura|Human whipworm|CDW57881 100 Trichuris suis|Whipworm|KFD68196 Trichuris trichiura|Human whipworm|CDW55439 100 Trichuris suis|Whipworm|KHJ40964 100 Trichuris suis|Whipworm|KFD50001 Trichinella pseudospiralis|Trichinella roundworm|KRX87365 Trichinella pseudospiralis|Trichinella roundworm|KRX87370 Trichinella pseudospiralis|Trichinella roundworm|KRX87366 Trichinella pseudospiralis|Trichinella roundworm|KRY89154 100 Trichinella pseudospiralis|Trichinella roundworm|KRX87369 Trichinella pseudospiralis|Trichinella roundworm|KRY69105 Trichinella pseudospiralis|Trichinella roundworm|KRY69104 Trichinella pseudospiralis|Trichinella roundworm|KRY69106 Trichinella pseudospiralis|Trichinella roundworm|KRZ15873 Trichinella pseudospiralis|Trichinella roundworm|KRX87367 97 Trichinella zimbabwensis|Trichinella roundworm|KRZ13953 Trichinella zimbabwensis|Trichinella roundworm|KRZ13952 97 Trichinella papuae|Swine roundworm|KRZ71139 Trichinella papuae|Swine roundworm|KRZ71142 Trichinella papuae|Swine roundworm|KRZ71140 Trichinella papuae|Swine roundworm|KRZ71141 99 Trichinella patagoniensis|Trichinella roundworm|KRY10753 Trichinella patagoniensis|Trichinella roundworm|KRY10755 Trichinella patagoniensis|Trichinella roundworm|KRY10754 Trichinella spiralis|Trichinella roundworm|XP 003374666 Trichinella nelsoni|Trichinella roundworm|KRX17501 Trichinella nelsoni|Trichinella roundworm|KRX17502 Trichinella spiralis|Trichinella roundworm|KRY38903 Trichinella spiralis|Trichinella roundworm|KRY38904 Trichinella sp. T8|Trichinella roundworm|KRZ87599 99 Trichinella sp. T9|Trichinella roundworm|KRX59745 Trichinella sp. T6|Trichinella roundworm|KRX79063 Trichinella murrelli|Trichinella roundworm|KRX41628 Trichinella sp. T6|Trichinella roundworm|KRX79064 Trichinella murrelli|Trichinella roundworm|KRX41626 Trichinella murrelli|Trichinella roundworm|KRX41627 98 Trichinella murrelli|Trichinella roundworm|KRX41631 Trichinella britovi|Trichinella roundworm|KRY53687 Trichinella nativa|Trichinella roundworm|KRZ52384 Trichinella britovi|Trichinella roundworm|KRY53685 Trichinella nativa|Trichinella roundworm|KRZ52381 100 Trichinella britovi|Trichinella roundworm|KRY53688 Trichinella nativa|Trichinella roundworm|KRZ52380 Trichinella britovi|Trichinella roundworm|KRY53684 Trichinella nativa|Trichinella roundworm|KRZ52383 Trichinella nativa|Trichinella roundworm|KRZ52382 Pristionchus pacificus|Nematode|PDM69563 Teladorsagia circumcincta|Brown stomach worm|PIO69036 Dictyocaulus viviparus|Lungworm|KJH43833 100 100 Ancylostoma caninum|Dog hookworm|RCN33658 100 Ancylostoma ceylanicum|Hookworm|EYC46005 100 Diploscapter pachys|Nematode|PAV61018 Caenorhabditis latens|Nematode|OZF91865 98 100 Caenorhabditis remanei|Nematode|OZF99260 gtl-2 100 Caenorhabditis remanei|Nematode|XP 003107995 100 98 Caenorhabditis elegans|Nematode|gtl-2 CE40563 Caenorhabditis nigoni|Nematode|PIC34278 99 100 Caenorhabditis briggsae|Nematode|CAP26317.2 100 Caenorhabditis briggsae|Nematode|XP 002633316 Strongyloides ratti|Rat roundworm|XP 024504814 100 Steinernema carpocapsae|Entomopathogenic roundworm|TKR95141 Dracunculus medinensis|Guinea worm|VDN58044 Anisakis simplex|Marine roundworm|VDK42691 100 Toxocara canis|Dog roundworm|KHN79092 100 Toxocara canis|Dog roundworm|VDM44510 Thelazia callipaeda|Eye roundworm|VDN01629 Loa loa|Filarial eye roundworm|XP 020302521 100 Acanthocheilonema viteae|Rodent filarial roundworm|VBB25615 Onchocerca ochengi|Bovine roundworm|VDK63290 98 100 Onchocerca flexuosa|River blindness eye roundworm| VDP13005 Onchocerca flexuosa|River blindness eye roundworm|OZC12680 95 Wuchereria bancrofti|Elephantiasis roundworm|VDM18860 Brugia pahangi|Zoonotic lymphatic filarial roundworm|VDN83289 100 Brugia timori|Timor roundworm|VDO30096 Brugia malayi|Elephantiasis roundworm|CDQ02415 Brugia malayi|Elephantiasis roundworm|XP 001901231 Ancylostoma ceylanicum|Hookworm|EPB67786 100 Ancylostoma ceylanicum|Hookworm|EYB91093 100 Nippostrongylus brasiliensis|Rat hookworm|VDL83792 gtl-1 100 Haemonchus placei|Large stomach worm|VDO51628 Pristionchus pacificus|Nematode|PDM75278 100 100 Pristionchus pacificus|Nematode|PDM64775 Diploscapter pachys|Nematode|PAV79744 97 100 Diploscapter pachys|Nematode|PAV84897 Caenorhabditis elegans|Nematode|gtl-1 CE33754 100 Caenorhabditis brenneri|Nematode|EGT50227 100 Caenorhabditis briggsae|Nematode|XP 002633309 Caenorhabditis briggsae|Nematode|CAP26324.2 Caenorhabditis nigoni|Nematode|PIC34271 Caenorhabditis remanei|Nematode|POM45300 Caenorhabditis nigoni|Nematode|PIC34272 96 Caenorhabditis remanei|Nematode|XP 003107819 Dictyocaulus viviparus|Lungworm|KJH53513 99 Angiostrongylus costaricensis|Rat lungworm|VDM52884 Ancylostoma caninum|Dog hookworm|RCN42281 100 100 Ancylostoma ceylanicum|Hookworm|EYC21527 Nippostrongylus brasiliensis|Rat hookworm|VDL62851 Heligmosomoides polygyrus|Rodent intestinal roundworm|VDO88774 Haemonchus placei|Large stomach worm|VDO45564 100 Haemonchus contortus|Barber s pole worm|CDJ90594 Enterobius vermicularis|Human pinworm|VDD91528 Dracunculus medinensis|Guinea worm|VDN56541 99 gon-2 Anisakis simplex|Marine roundworm|VDK52174 100 Toxocara canis|Dog roundworm|KHN71877 Thelazia callipaeda|Thelizia eye worm|VDN07735 Acanthocheilonema viteae|Rodent filarial roundworm|VBB32995 100 Loa loa|Filarial eye roundworm|XP 020305655 97 Wuchereria bancrofti|Elephantiasis roundworm|VDM09111 98 Brugia pahangi|Zoonotic lymphatic filarial roundworm|VDN94084 Brugia malayi|Elephantiasis roundworm|CDQ04279 Brugia malayi|Elephantiasis roundworm|VIO96916 Steinernema carpocapsae|Entomopathogenic roundworm|TKR89621 100 Steinernema carpocapsae|Entomopathogenic roundworm|TKR93888 Strongyloides ratti|Rat roundworm|XP 024507084 Strongyloides ratti|Rat roundworm|XP 024509240 100 Strongyloides ratti|Rat roundworm|XP 024509050 Pristionchus pacificus|Nematode|PDM75839 Diploscapter pachys|Nematode|PAV63198 Caenorhabditis elegans|Nematode|gon-2 CE30390 Caenorhabditis nigoni|Nematode|PIC52068 Duplications before 100 100 Caenorhabditis briggsae|Nematode|CAP31489.2 100 Caenorhabditis brenneri|Nematode|EGT32766 Cniadaria-Bilateria split Caenorhabditis remanei|Nematode|XP 003112078 100 Caenorhabditis remanei|Nematode|OZF79912 Figure S15 100 TRPS Aurelia|Moon jelly|Seg1803.3 transcript id Seg1803.3|GoldUCD|mRNA.D3Y31 Priapulus caudatus|Penis worm|XP 014669422 Homo sapiens|Human|TRPM8 CCDS33407.1 100 Homo sapiens|Human|TRPM2 CCDS13710.1 100 Homo sapiens|Human|TRPM5 CCDS31340.1 100 Homo sapiens|Human|TRPM4 CCDS33073.1 100 Hydra vulgaris|Fresh-water polyp|XP 012565257 99 Aurelia|Moon jelly|Seg1831.8 transcript id Seg1831.8|GoldUCD|mRNA.D3Y31 Nematostella vectensis|Starlet sea anemone|Nemve1|248535 100 Pocillopora damicornis|Lace coral|pdam 00009856-RA β 100 100 Stylophora pistillata|Hood coral|Spis4675 SpisGene4675 Fungia spp.|Coral|ffun1.m4.19256.m1 100 100 Goniastrea aspera|Stony coral|gasp1.m3.30302.m1
99 Porites lutea|Small polyp stony coral|plut2.m8.2561.m1 Galaxea fascicularis|Galaxy coral|gfas1.m1.6599.m1 100 99 Acropora digitifera|Coral|adi v1.10270 100 Acropora tenuis|Coral|aten 0.1.m1.1851.m1 Pocillopora damicornis|Lace coral|pdam 00007420-RA 100 Stylophora pistillata|Hood coral|Spis14035.t1 SpisGene14035
100 Fungia spp.|Coral|ffun1.m4.21870.m1 Galaxea fascicularis|Galaxy coral|gfas1.m1.11287.m1 Goniastrea aspera|Stony coral|gasp1.m3.6245.m1 Limulus polyphemus|American horseshoe crab|XP 022255565 99 Stegodyphus mimosarum|African social velvet spider|KFM65832 100 100 Araneus ventricosus|Orb-weaver spider|GBM10676 Parasteatoda tepidariorum|American house spider|XP 015907052
100 Tigriopus californicus|Copepod|TRY76337 100 99 Eurytemora affinis|Copepod|XP 023322531 100 Tigriopus californicus|Copepod|TRY77421 99 Amphibalanus improvisus|Bay barnacle|QCO69752 Hyalella azteca|Freshwater amphipod|XP 018027820 100 Penaeus vannamei|Whiteleg shrimp|ROT84591 100 100 Penaeus vannamei|Whiteleg shrimp|XP 027222949 100 Penaeus vannamei|Whiteleg shrimp|ROT85499 Hydra vulgaris|Fresh-water polyp|XP 012554388
100 Hydra vulgaris|Fresh-water polyp|XP 012562662 100 Hydra vulgaris|Fresh-water polyp|XP 012561335 Aiptasia|Sea anemone|AIPGENE12924 Nematostella vectensis|Starlet sea anemone|Nemve1|85952 99 Pocillopora damicornis|Lace coral|pdam 00014050-RA
100 Goniastrea aspera|Stony coral|gasp1.m3.26670.m1 100 Fungia spp.|Coral|ffun1.m4.18855.m1 100 Aiptasia|Sea anemone|AIPGENE19094 α 100 Nematostella vectensis|Starlet sea anemone|Nemve1|102167
99 Porites lutea|Small polyp stony coral|plut2.m8.21682.m1 Pocillopora damicornis|Lace coral|pdam 00020220-RA 100 99 100 Goniastrea aspera|Stony coral|gasp1.m3.6037.m1 100 Fungia spp.|Coral|ffun1.m4.18290.m1 Homo sapiens|Human|TRPM3 CCDS6634.1 100 Homo sapiens|Human|TRPM1 CCDS10024.2 100 Homo sapiens|Human|TRPM7 CCDS42035.1 100 Homo sapiens|Human|TRPM6 CCDS6647.1 Priapulus caudatus|Penis worm|XP 014671784 Limulus polyphemus|American horseshoe crab|XP 022248148 100 100 Centruroides sculpturatus|Arizona bark scorpion|XP 023237252 100 Ixodes scapularis|Deer tick|XP 029824440 Daphnia magna|Water flea|KZS13223
100 100 Daphnia pulex|Water flea|EFX80451 100 Daphnia magna|Water flea|KZS13280 Armadillidium vulgare|Roly-poly|RXG60922 Penaeus vannamei|Whiteleg shrimp|XP 027220225 100 100 Penaeus vannamei|Whiteleg shrimp|XP 027220241 100 100 Cancer borealis|Jonah crab|APG53781 100 Homarus americanus|American lobster|APG53788 Orchesella cincta|Belted springtail|ODM97521 Blattella germanica|German cockroach|PSN39268 Zootermopsis nevadensis|Termite|XP 021919466 100 100 Cryptotermes secundus|West Indian drywood termite|XP 023715316 100 Cryptotermes secundus|West Indian drywood termite|XP 023710445 99 Frankliniella occidentalis|Western flower thrips|XP 026274853 100 Pediculus humanus corporis|Body louse|XP 002423257 Laodelphax striatellus|Small brown planthopper|RZF33234 100 Nilaparvata lugens|Brown planthopper|XP 022186364 Bemisia tabaci|Silverleaf whitefly|XP 018904967 Sipha flava|Yellow sugarcane aphid|XP 025425746 Rhopalosiphum maidis|Corn leaf aphid|XP 026809557
100 Aphis gossypii|Melon and cotton aphid|XP 027836600 Melanaphis sacchari|Sugarcane aphid|XP 025203880 100 Acyrthosiphon pisum|Pea aphid|XP 003245193
99 Diuraphis noxia|Russian wheat aphid|XP 015367699 99 Myzus persicae|Green peach aphid|XP 022180245 Agrilus planipennis|Emerald ash borer|XP 025833357 Nicrophorus vespilloides|Buring beetle|XP 017773716 Nicrophorus vespilloides|Boreal carrion beetle|XP 017773713 100 Nicrophorus vespilloides|Boreal carrion beetle|XP 017773706 Nicrophorus vespilloides|Boreal carrion beetle|XP 017773711 100 Onthophagus taurus|Taurus scarab|XP 022904186 100 Onthophagus taurus|Taurus scarab|XP 022904266 Asbolus verrucosus|Desert ironclad beetle|RZC40720 Tribolium castaneum|Red flour beetle|XP 015837221 100 100 Tribolium castaneum|Red flour beetle|XP 015837222 Tribolium castaneum|Red flour beetle|XP 015837215 100 Aethina tumida|Small hive beetle|XP 019874384 Dendroctonus ponderosae|Mountain pine beetle|ENN80022 100 100 Dendroctonus ponderosae|Mountain pine beetle|XP 019755236
100 100 Anoplophora glabripennis|Asian longhorned beetle|XP 023311041
100 Diabrotica virgifera virgifera|Western corn rootworm|XP 028136863 100 Leptinotarsa decemlineata|Colorado potato beetle|XP 023017091 Ctenocephalides felis|Cat flea|XP 026479982 98 Clunio marinus|Midge|CRL00240 Anopheles darlingi|Mosquito|ETN57805
100 100 Anopheles gambiae senso stricto|Mosquito|XP 308919.3 100 100 Anopheles sinensis|Mosquito|KFB51525 100 Aedes aegypti|Yellow fever mosquito|XP 021698933 Aedes albopictus|Asian tiger mosquito|XP 029735731 100 100 Aedes albopictus|Asian tiger mosquito|XP 029734607 100 99 Aedes albopictus|Asian tiger mosquito|KXJ83914 Drosophila melanogaster|Common fruit fly|Trpm Lucilia cuprina|Australian sheep blowflow|XP 023291853 100 100 Stomoxys calcitrans|Stable fly|XP 013109884 100 Musca domestica|Housefly|XP 011296059 100 100 Bactrocera latifrons|Solanum fruit fly|XP 018803596 Zeugodacus cucurbitae|Melon fly|XP 011178713 100 Bactrocera dorsalis|Oriental fruit fly|XP 019844435 100 100 Ceratitis capitata|Mediterranean fruit fly|XP 012154700 100 Bactrocera oleae|Olive fruit fly|XP 014099935 Hyposmocoma kahamanoa|Moth|XP 026320800 Manduca sexta|Tobacco hornworm Carolina sphinx moth|QDR51034 100 Manduca sexta|Tobacco hornworm Carolina sphinx moth|XP 030030234 100 Bombyx mandarina|Wild silkmoth|XP 028025209 100 100 Bombyx mori|Domestic silkmoth|XP 012551960 Trichoplusia ni|Cabbage looper moth|XP 026740408 Spodoptera litura|Tobacco cutworm moth|XP 022827232 100 98 Helicoverpa armigera|Cotton bollworm moth|PZC87367 100 Helicoverpa armigera|Cottom bollworm moth|XP 021181239 Chilo suppressalis|Asiatic rice borer moth|RVE51394 Galleria mellonella|Great wax moth|XP 026762983 100 Amyelois transitella|Navel orangeworm snout moth|XP 013187551 Danaus plexippus|Monarch butterfly|QDQ16920 Bicyclus anynana|Squinting bush brown butterfly|XP 023939913 Vanessa tameamea|Kamehameha butterfly|XP 026491684 Heliconius melpomene|Postman butterfly|QDR50959 Pieris rapae|Small cabbage white butterfly|XP 022128471 Papilio polytes|Common Mormon butterfly|XP 013137248 Papilio machaon|Old World swallowtail butterfly|KPJ09541 100 99 Papilio machaon|Old World swallowtail butterfly|XP 014365775 100 Papilio xuthus|Asian swallowtail butterfly|XP 013162535 Papilio xuthus|Asian swallowtail butterfly|KPJ04070 Neodiprion lecontei|Redhead pine sawfly|XP 015512476 100 Neodiprion lecontei|Redhead pine sawfly|XP 015512479 Orussus abietinus|Parasitic wood wasp|XP 012280557 100 Orussus abietinus|Parasitic wood wasp|XP 012280565 Cephus cinctus|Wheat stem sawfly|XP 015594698
100 Cephus cinctus|What stem sawfly|XP 015594691 Cephus cinctus|Wheat stem sawfly|XP 015594697 100 Diachasma alloeum|Parisitoid wasp|XP 015112330 100 Fopius arisanus|Braconid wasp|XP 011307041 100 Microplitis demolitor|Parasitoid wasp|XP 014296021 100 Microplitis demolitor|Parasitoid wasp|XP 008545401 Trichogramma pretiosum|Polyphagous wasp|XP 014228063 100 100 Trichogramma pretiosum|Polyphagous wasp|XP 014228070
100 Copidosoma floridanum|Parasitoid wasp|XP 023245943 Ceratosolen solmsi marchali|Fig wasp|XP 011494034 100 100 Nasonia vitripennis|Parasitoid wasp|XP 016845126 100 Trichomalopsis sarcophagae|Wasp|OXU23397 Ceratina calcarata|Spurred ceratina bee|XP 026670292 100 Ceratina calcarata|Spurred ceratina bee|XP 026670488 Megachile rotundata|Alfalfa leafcutting bee|XP 012140911 100 100 100 Osmia bicornis bicornis|Red mason bee|XP 029053463 Habropoda laboriosa|Blueberry digger bee|KOC68432 96 Eufriesea mexicana|Mexican orchid bee|XP 017763457 96 Melipona quadrifasciata|Mandaaia bee|KOX68915 Bombus impatiens|Common eastern bumblebee|XP 012238938 97 100 100 Bombus terrestris|Buff-tailed bumblebee|XP 012166409
100 Apis cerana cerana|Eastern honey bee|XP 028521981 100 Apis cerana cerana|Eastern honey bee|PBC31881
100 100 Apis florea|Red dwarf honey bee|XP 003695376
100 Apis mellifera|European honey bee|XP 016769201 100 Apis dorsata|Giant honey bee|XP 006617666 Polistes dominula|European paper wasp|XP 015190771 100 Polistes canadensis|Red paper wasp|XP 014600553 Linepithema humile|Argentine ant|XP 012234779
99 Pseudomyrmex gracilis|Slender twig ant|XP 020300482 100 Pseudomyrmex gracilis|Slender twig ant|XP 020300486 Harpegnathos saltator|Jerdon s jumping ant|XP 019700199.2 100 Dinoponera quadriceps|South American ant|XP 014480910 Nylanderia fulva|Tawny crazy ant|XP 029159541
100 Formica exsecta|Narrow-headed ant|XP 029678003 100 100 Camponotus floridanus|Florida carpenter ant|XP 025271119 Ooceraea biroi|Clonal raider ant|EZA58689 100 Ooceraea biroi|Clonal raider ant|XP 011331801 Pogonomyrmex barbatus|Red harvester ant|XP 025073334 Monomorium pharaonis|Pharaoh ant|XP 012538403 Vollenhovia emeryi|Ant|XP 011879164 100 Temnothorax curvispinosus|Ant|XP 024882761 100 Temnothorax longispinosus|Ant|TGZ50551 100 Wasmannia auropunctata|Little fire ant|XP 011703736 Cyphomyrmex costatus|Fungus growing ant|KYN00283 99 Trachymyrmex zeteki|Fungus-growing ant|KYQ46528 100 100 Trachymyrmex zeteki|Fungus growing ant|XP 018316097 Trachymyrmex cornetzi|Fungus-growing ant|XP 018375041 100 Trachymyrmex septentrionalis|Leaf-cutter ant|XP 018348579
100 Acromyrmex echinatior|Leaf-cutting ant|XP 011055821 Duplications before Atta colombica|Leaf-cutter ant|XP 018049373 Cniadaria-Bilateria split 100 Atta cephalotes|Leaf-cutter ant|XP 012064138 Figure S16 5
Carcharodon carcharias|Great white shark|ScRDnwc 40374 6376172-snap-gene-36.2-mRNA-1
Chiloscyllium punctatum|Brownbanded bamboo shark|Chipu0004516.p1
Erpetoichthys calabaricus|Freshwater snakefish|XP 028646809.1
Xiphophorus couchianus|Monterrey flatyfish|XP 027879946.1
Xiphophorus maculatus|Southern platyfish|XP 023192248.1
Scyliorhinus torazame|Cloudy catshark|Scyto0000828.p1
Gambusia affinis|Western mosquitofish|PWA31201.1 2 Latimeria chalumnae|Coelacanth|XP 014344856.1
Xiphophorus maculatus|Southern platyfish|XP 023193351.1
Callorhinchus milii|Ghostshark|XP 007902997.1 Rhincodon typus|Whale shark|XP 020389255.1 Poecilia mexicana|Shortfin molly|XP 014850901.1
Poecilia mexicana|Shortfin molly|XP 014869423.1 Gallus gallus|Chicken|TRPM5 E1BXA4 CHICK Cyprinodon variegatus|Sheepshead minnow|XPFundulus 015241567.1 heteroclitus|Mummichog killifish|XP 021180116.1 Homo sapiens|Human|TRPM5 CCDS31340.1
Poecilia formosa|Amazon molly|XP 016522860.1 Poecilia latipinna|Sailfin molly|XP 014888921.1 Fundulus heteroclitus|Mummichog killifish|XP 021180117.1
Mus musculus|Mouse|Trpm5 CCDS52462.1 Poecilia mexicana|Shortfin molly|XP 014869424.1 Poecilia formosa|Amazon molly|XP 016519296.1 Poecilia latipinna|Sailfin molly|XP 014879157.1 Austrofundulus limnaeus|Aplocheilidan fish|XP 013872184.1 Kryptolebias marmoratus|Mangrove killifish|XP 017279883.1 Poecilia formosa|Amazon molly|XP 007577662.1
Poecilia mexicana|Shortfin molly|XP 014832581.1 Poecilia formosa|Amazon molly|XP 016519295.1 Haplochromis burtoni|Burton s mouthbrooder fish|XP 005918368.1 Poecilia formosa|Amazon molly|XP 007577647.1 Poecilia reticulata|Gup Gambusia affinis|Western mosquitofish|PWA31202.1 Oryzias melastigma|Marine medaka ricefish|XP 024141945.1 Nothobranchius furzeri|Turquoise killifish|XP 015822163.1
Poecilia latipinna|Sailfin molly|XP 014888953.1
Poecilia reticulata|Guppy|XP 008401657.1
Astatotilapia calliptera|Eastern river bream|XP 026001155.1
Poecilia reticulata|Guppy|XP 008402158.1 Parambassis ranga|Indian glassy fish|XP 028249051.1
Pundamilia nyererei|Victorian cichlid|XP 005723388.1 Neolamprologus brichardi|Princess cichlid|XP 006788736.1 Oryzias javanicus|Javanese ricefish|RVE58090.1 Oryzias latipes|Japanese ricefish|XP 023806259.1 1 .
Gouania willdenowi|Blunt-snouted clingfish|XP 028292479.1
Oreochromis niloticus|Nile talapia|XP 005453113.1 Amphiprion ocellaris|Ocellaris clownfish|XP 023136566.1 Maylandia zebra|Zebra mbuna|XP 014263733.2 Salarias fasciatus|Jewelled blenny|XP 029968357.1 py|XP 008402155.1
Salarias fasciatus|Jewelled blenny|XP 029968389.1
Tetraodon nigroviridis|Green spotted pufferfish|CAG09283.1Stegastes partitus|Bicolor damselfish|XP 008285893.1
Takifugu rubripes|JapaneseTakifugu flavidus|Yellowbelly pufferfish|XP 029707103.1 pufferfish|TWW80709.1
Larimichthys crocea|Large yellow croaker|XP 027147042.1
ese pufferfish|XP 011608202.2
Acipenser ruthenus|Sterlet sturgeon|RXM31127.1 Lepisosteus oculatus|Spotted gar|XP 015193971.1 Scleropages formosus|Asian arowana|XP 018608238.1
Paramormyrops kingsleyae|Elephantfish|XP 023660171.1 Pygocentrus nattereri|Red-bellied piranha|XP 017571910.1
Electrophorus electricus|Electric eel|XP 026869621.1
Danionella translucida|Glassfish|TRY93569.1
Labeo rohita|Rohu|RXN26475.1 Perca flavescens|Yellow perch|XP 028446832.1 Labeo rohita|Rohu|RXN17181.1 Larimichthys crocea|Large yellow croaker|TMS06678.1 Carassius auratus|Goldfish|XP 026070741.1 Sinocyclocheilus grahami|Golden-line barbel|XP 016102616.1 Sinocyclocheilus rhinocerous|Barbel|XP 016367451.1
Sinocyclocheilus anshuiensis|Barbel|XP 016349168.1
Cyprinus carpio|Common carp|KTF88424.1 Liparis tanakae|Tanaka s snailfish|TNN53990.1Perca flavescens|Yellow Carassius auratus|Goldfish|XP 026123718.1 Sinocyclocheilus anshuiensis|Barbel|XP 016352581.1
Sinocyclocheilus grahami|Golden-line barbel|XP 016142717.1
Sinocyclocheilus rhinocerous|Barbel|XP 016396503.1 Esox lucius|Northern pike|XP 010894223.1 Oncorhynchus mykiss|Rainbow trout|XP 021442542.1
Salmo salar|Atlantic salmon|XP 013983149.1
Anabas testudineus|Climbing perch|XP 026232352.1Cottoperca gobio|Thornfish|XP 029314727.1 Salmo trutta|Brown trout|XP 029576425.1 Salmo salar|Atlantic salmon|XP 014041822.1 49956.1
Collichthys lucidus|Big head croaker|TKS67317.1 Gadus morhua|Atlantic cod|XP 030233186.1
Myripristis murdjan|Pinecone soldierfish|XP 029904281.1 Mastacembelus armatus|Zig-zag eel|XP 026150464.1 Sphaeramia orbicularis|Orbiculate cardinalfish|XP 030015679.1 Lates calcarifer|Barramundi|XP 018538869.1 Echeneis naucrates|Sharksucker remora|XP 029354367.1 us|Javanese ricefish|RVE74198.1 Seriola lalandi|Yellowtail amberjack|dorsalis XP 023248927.1
Monopterus albus|Asian swamp eel|XP 020450341.1 Seriola dumerili|Greater amberjack|XP 022603862.1
Paralichthys olivaceus|Olive flounder|XP 019945245.1 Cynoglossus semilaevis|Tongue sole|XP 008308372.1
Scophthalmus maximus|Turbot|AWP01207.1 Seriola lalandi|Yellowtail amberjack|dorsalis XP 023284369.1 Paralichthys olivaceus|Olive flounder|XP 019955010.1 Cynoglossus semilaevis|Tongue sole|XP 024919767.1 Boleophthalmus pectinirostris|Great blue spotted mudskipper|XP 020794683.1 Betta splendens|Betta fish|XP 029000167.1 Betta splendens|Betta fish|XP 028993466.1 fish|XP 029318391.1 y|XP 0299 Anabas testudineus|Climbing perch|XP 026233556.1 Monopterus albus|Asian swamp eel|XP 020464475.1
Mastacembelus armatus|Zig-zag eel|XP 026175956.1 Echeneis naucrates|SharksuckerSeriola dumerili|Greater remora|XP 029385890.1 amberjack|XP 022597076.1 Hippocampus comes|Tiger tail seahourse|XP 019742094.1 007372.p1 Takifugu rubripes|Japan
Oryzias latipes|Japanese ricefish|XP 023808229.1 to0 Oryzias melastigma|Marine medaka ricefish|XP 024151974.1 o|Thorn rofundulus limnaeus|Aplocheilidan fish|XP 013872441.1 perch|TDH06962.1 Oryzias javanic Scophthalmus maximus|Turbot|AWP12995.1 Sp Nothobranchius furzeri|Turquoise killifish|XP 015811826.1rinodon variegatus|Sheepshead minnow|XP 015253051 Kryptolebias marmoratus|Mangrove killifish|XP 017269766.1 33073.1 haeramia orbicularis|Orbiculate cardinalfish|XP 029981390.1 Aust Cyp buna|XP 023008338.1 s lucidus|Big head croaker|TKS69525.1 Poecilia reticulata|Guppy|XP 008403907.1
Poecilia mexicana|Shortfin molly|XP 014828169.1 Labrus bergylta|BallanLates calcarifer|Barramundi|XP wrasse|XP 020506803.1 018527531.1 CCDS Poecilia latipinna|Sailfin molly|XP 014916486.1 ra m Hippocampus comes|Tiger tail seahourse|XPLabrus 019737856.1 bergylta|Ballan wrasse|XP 020506815.1 Poecilia formosa|Amazon molly|XP 007574618.1 Notothenia coriiceps|Black rockcod|XP 010773286.1 Labrus bergylta|Ballan wrasse|XP 029136475.1 Cottoperca gobi |Zeb Myripristis murdjan|Pinecone soldierfish|XP 029936492.1 fasciatus|Jewelled blenn Collichthy TRPM4
Larimichthys crocea|Large yellow croaker|TMS13148.1
Oncorhynchus tshawytscha|Chinook salmon|XP 024262434.1 Larimichthys crocea|Large yellow croaker|XP 019128921.2
Parambassis ranga|Indian glassy fish|XP 028257103.1 1
Salarias 2918.1 Oncorhynchus kisutch|Coho salmon|XP 020327172.1 Gouania willdenowi|Blunt-snouted clingfish|XP 028328730.1
Oreochromis niloticus|Nile talapia|XP 005455786.1
Oncorhync Neolamprologus brichardi|Princess cichlid|XP 006794016.1 Gadus morhua|Atlantic cod|XP 030198678.1 Maylandia zebra Oncorhynchus mykiss|Rainbow trout|XP 021435737.1 Pundamilia nyererei|Victorian cichlid|XP 005749679.1
Haplochromis burtoni|Burton s mouthbrooder fish|XP 014191818.1 1447.1 orhinus torazame|Cloudy catshark|Scy Mus musculus|Mouse|Trpm4 CCDS52245.1 |XP 012681004. hus nerka|Sockeye salmon|XP 029507347.1 g Homo sapiens|Human| Sinocyclocheilus grahami|Golden-line barbel|XP 016108589.1 cyli S
Salmo salar|Atlantic salmon|XP 014021288.1 Latimeria chalumnae|Coelacanth|XP 005990988.1 Sinocyclocheilus anshuiensis|Barbel|XP 016324834.1 Erpetoichthys calabaricus|Freshwater snakefish|XP 028669413.1
Lepisosteus oculatus|Spotted gar|XP 015208857.1 bellied piranha|XP 01755 Salmo trutta|Brown trout|XP 029557805.1 3.1 Paramormyrops kingsleyae|Elephantfish|XP 023681832.1 Sinocyclocheilus rhinocerous|Barbel|XPEso 016411350.1 ed- Scleropages formosus|Asian arowana|XP 029102689.1 515
x lucius|Northern pike|XP 028971788.1 Scleropages formosus|Asian arowana|KPP62917.1 Paramormyrops kingsleyae|Elephantfish|XP 023654213.1
Scleropages formosus|Asian arowana|XP 029109716.1 2.1
Pangasianodo Cyprinus carpio|Common carp|XP 018952431.1 Scleropages formosus|Asian arowana|KPP64274.1 Carassius auratus|Goldfish|XP 026074779.1 197 1.1 Denticeps clupeoides|Denticle herring|XP 028841108.1 ectricus|Electric eel|XP 02687 xicanus|Blind cave fish|XP 022532779.1 97 el Clupea harengus|Atlantic herring|XP 012681005.1 me Cyprinus carpio|Common carp|KTG36711.1 Clupea harengus|Atlantic herrin 16091 Tachysurus fulvidraco|Yellowhead catfish|XPCarassius 027006707.1 auratus|Goldfish|XP 026126908.1 Tachysurus fulvidraco|Yellowhead catfish|XP 027011568.1 n hypophthalmus|Irridescent shark catfish|X anax Pygocentrus nattereri|R 18931398.1 Anabarilius grahami ty bel|XP 01609 0 |XP 0 As horus electricus|Electric eel|XP 026852630.1 Bagarius yarrelli|Giant devil catfish|TSK92937.1 ectrophorus El op Ictalurus p Electrophorus electricus|Electric eel|XP 026870496.1
Electr Astyanax mexicanus|Blind cave fish|XP 022519978.1 Labeo rohita|Rohu|RXN16987.1 Py Danionella translucida|Glassfish|TRY59936.1 Astyanax gocentrus unctatus|Freshwater catfish|XP 017324684.1 Anabarilius grahami|Kanglang fish|ROI1
| Carassius auratus|Goldfish|XP 026064209.1 Golden-line barbel Kangla nocyclocheilus rhinocerous|Barbel|XP 016395393.1 Si Electrophorus electricus|Electric eel|XP 026866648.1 arpio|Common carp|XP mexicanus|Blind cave fish|XP 022536377.1 nat Sinocyclocheilus anshuiensis|Barbel|XP c 016302443.1 ng fish|ROL46396.1 inocyclocheilus grahami|Golden-line barbel|XP 016124555.1 S Clupea harengus|Atlantictereri|Red-bellied herring|XP piranha|XP 012682771.1 017547069.1 Carassius auratus|Goldfish|XP 026062021.1lus grahami| Denticeps Clupeaclupeoides|Denticle harengus|Atlantic herring|XP herring|XP 028847051.1 012689405.1 Cyprinus hei yprinus carpio|Commonloc carp|KTF96250.1 C
Sc Scleropages formosus|Asian arowana|KPP65667.1 P 026 Sinocyclocheilus grahami|Golden-line bar 024289571.1 Paramormyrops kingsleyae|Elephantfish|XP 023695736.1 leropages formosus|Asian arowana|XP 018580884.1 XP Sinocyc t|XP 021481715.1 u 802301.1 on| Erpetoichthys calaba Sinocyclocheilus anshuiensis|Barbel|XP 016343260.1 tro lm Sinocyclocheilus rhinocerous|Barbel|XP 016397156.1 Erpet Sinocyclocheilus rhinocerous|Barbel|XP 016397154.1 ok sa o Lepisosteus oculatus|Spott oichthys calabaricus|Freshwater sn Salmo trutta|Brown trout|XP 029582905.1 in Esox lucius|Northern pike|XP 028971216.1 iss|Rainbow Salmo salar|Atlantic salmon|XP 014047737.1 yk Oncorhynchus tshawytscha|Chinook salmon|XP 024278283.1 ricus|Freshwa Oncorhynchus kisutch|Coho salmon|XP 020341710.1 hynchus mykiss|Rainbow trout|CDQ77664.1 Chiloscyllium Oncorhynchusor nerka|Sockeye salmon|XP 029491142.1
Homo sapiens|Human Onc on|XP 014047663.1 77.1 Oncorhynchus m t m M er snakefish|XP 028662026.1 al XP 010904481.1 999 1 us musculu ed 8 punct Lati Gallus gallus|Chicken|TRP Oncorhynchus mykiss|Rainbow trout|XP 021481298.1 1 ga tic s 145. meria chalumn n pike| r|XP Oncorhynchus tshawytscha|Ch a Scyliorhinustum|Brownbanded tor bamboo shark|Chipu0018262.p1 a XP 0198 Rhincodon typus|Whale shark|XP 02037481 kefish|XP 028663964.1 Oncorhynchus kisutch|Coho salmon|XP 020328543.1 s 01521 P 024278305.1 |Mouse|Trp Salvelinus alpinus|Arctic char|XP 023994379.1 |X |TRPM2 CCDS13710.1 on 4648. Salmo trutta|Brown trout|XP 029584352.1 on|XP 029491 ae rthern pike| m Callorhinchus |Coelacanth|XP 014345762.1 Salmo salar|Atla lucius|Northern sal azam 1 x 719.1 m so 2 CCDS48611.1 E us|No Callorhinchus milii|Ghostshark|XP 007888802.1 M2 F1NGK6 CHICK ci e|Cloudy catshark|Scyto0014884.p1 sox lucius|Northern pike|XP 019899961.2 E lu 21481 x lucius|Northern pike|XP 010863093.1cha|Chinook salm Eso Homo sapiens|Human|TRPM8mi CCDS33407.1 lii |Ghostshark|XP 0 Esox Mus musculus|Mouse Salmo trutta|Brown trout|XP 029582906. Lati Gallus gallu kisutch|Cohoiss|Rainbow salmon|XP trout|CDQ82256.1 020341779.1 m Salmo salar|Atlantic salmon|XPs 014047736.1k eria chalumnae|Coelacanth|XP 014345846.1 te cardinalfish|XP 029997367.1 hu a 1.1 Oncorhynchus nerka|Sockeye s mykiss|Rainbow trout|XP 0 1 |Chicken|TRP 0 Oncorhynchus tshawyts 7888801.1 nchus my |Orbicul 0. hy is | Oncorhync Oikopleura dioica|Tunicate|CBY10098Trpm8 CCDS48316.1 cor nchus icular On Oikopleura dioica|Tunic 02260943 Ciona intestinalis|Vase tunicate|XP M8 Oncorhy BranchiostomaBranchiostoma floridae|Florida belcheri|Lancelet|XPOikopleura lancelet|XP dioica|Tunicate|CBY19337 002592248 019640471 E1BWL7 CHICK ck|XP a Myripristis murdjan|Pinecone soldierfish|XP 029914144.1 Branchiostoma floridae|Florida lancelet|XP 002605080 Sphaeramia orb
Lates calcarifer|Barramundi|XP 018553920.1 oglossus semilaevis|Tongue atersole|XP amberj 024920264.1 di|Yellowtail amberjack|dorsalis XP 023262875.1 Bra a n Praesagittifera naikaiensis|Acoel flatworm|g10675.t1 te|CB Cyn 9740378.1 nchiostoma belcheri|Lancelet|XP 019638014 lala perch|XP 026206742.1 1 Echeneis naucrates|Sharksuckera remora|XP 029363555.1 Pra Y13016 ng esagittifera naikaiensis|Acoel 1 Seriol Pr 0 a 26692588 hourse|XP 0 esag Seriola dumerili|Gre 1 ittife cembelus armatus|Zig-zag eel|XP 026181144.1l sea Monopterusa albus|Asian swamp eel|XP 020463822.1 st ra naik er tai A Ma s testudineus|Climbi ig 27137391. cropo aba 37388.1 Gal a XP 0 1 ie An splendens|Betta fish|XPmes|T 029014782.1 27 1208.1 Porites lutea|Smallaxe polyp stonyra tencoral|plut2.m8nsis|Acoel flatworm|g14495.t1 a 0 a fa Bett 2831 Acropora digitifera|Coral|adiuis|Co flv1.10270atworm| pus co 0 scicularis|Galaxy coral|gfas1.m1.6599.m1 ad croaker|TKS88131.1 P Betta splendens|Betta fish|XP 029014779.1 G r ocam rgylta|Ballan wrasse|XPg he 020505240. oniastrea al|aten 0.1.m1.1851.m1 yellow croaker| g1 Bi Hipp us be ge 4113.t1 dus| yellow croaker|XP 5.1 22065782.1 Aurelia|Moon jelly|Seg1831.8 transcriptStylophora id pistillataSeg1831.8|GoldUCD|mRNA.D3Y31 br 0 aspera La ge P ichthys lucidus|Big head croaker|TKS92483.1 h|X |Lar y|XP 029946140.1 t-snouted clingfish|Xn XP 008288443.1 Nematostella vectensis|Starlet sea anemone|Nemve1|248535| Coll | 02312699 Pocillopora damicornis|Lace coral|pdamStony coral|gasp1.m3.3030 00009856-RA ocea 4 Fungia spp.|Coral|ffun1.m4.19256.m1 ollichthys luci cr Blun |XP C chthys crocea|Lar | h i ys lled blen is we |H Larim denowi lownf oo ll 7.1 d 72 coral Larimichth laris c 8 .25 |Bicolor damselfishel |S 61.m1 tus pis4675 SpisGene4675 Gouania wi rti Hyd pa laris|Oc Bran el ra 2.m Salarias fasciatus|Jetes chiostoma vf ulgaris|Fresh-water polyp| 1 .1 Stegas Goniastrea aspera|Stony coral|gasp1.m3.6245.m1 mphiprion oc Gala lori A hochromis polyacanthus|Spiny chromis sh|RVE69012.1damselfis 017295191 ine medakafi ricefish|XP 02412 xe dae nt ifish|XP 015824724.1 assis ranga|Indian glassyar fish|XP 028267277.1ill h|XP 1 S a fascicul |Fl Aca rice is . tylo o ese p rida hora pistillat Paramb avan 716460 1 aris|Gala lancelet|XP 002592165 J ove killif XP 0 us| |Turquoise k Oryzias latipes|Japanese ricefish|XP 023813623.1angr P 012 16447. Fungia sp 1256 eri 27 Pocillopora damicornis|Lace coral|pdamxy c 00007420-RA sh|X a|Hood coral|Spis14035.t javanic furz ifi o 5257 Oryzias melastigma|M ratus|M ral|gfas1.m1.11287.m1 9.1 p nchius 0 .|Co Oryzias illifish|XP 01299 s marmo ral|ffun1.m4.2 ia WA b chog k TRPS Nothobraole Krypt 1 Sp 1870.m1 us|Mummi isGe rn mosquitofish|P tyfish|XP 027879774.1 Austrofunduluslus heteroclitus|Mummichog limnaeus|Aplocheilidan kill fish|XP 013876440.1la n e14035 |Weste Fundu us heteroclitnis terrey f dul on Fun ia affi |M s nus Gambu uchia 4.1 Xiphophorus maculatus|Southern platyfish|XP 023205278.1
Xiphophorus co 7.1 Poecilia reticulata|Guppy|XPa|Amazon 008413217.1 molly|XP 016520142.169390 mos 012 Poecilia latipinna|Sailfinfor molly|XP 014877391.1 |XP 69723.1 9.1 upeoides|Denticle herring|XPing 028823396.126 Poecilia herr 2688695 ring|XP 01 clupeoides|Denticletlantic herring|XPr 02882339 Denticeps cl c he nti 975.1 Denticeps |Atla ectric eel|XP 0 us a harengus|A us|El lupe C areng |XP 022524 70690.1 ish 67 e f Clupea h P 02 phorus electrictereri|Red-bellied piranha|XP 017576002.1 X ro at ind cav fish| Elect us n entr Pygoc mexicanus|Bl ax devil catfish|TSK14512.1 s fulvidraco|Yellowhead catfish|XP 027019043.1 Astyan ru Tachysu garius yarrelli|Giant .1 Ba 4 8.1 us|Freshwaterlang catfish|XP fish|ROL48626.1 017339465.173 368 269.1 nctat 16 6 Pangasianodon hypophthalmus|Irridescent|Kang fish|TRY9846 shark 0 cat |XP talurus pu grahami arbel Ic us cida|Glass barili anslu erous|B Ana a tr
Danionell
Sinocyclocheilus rhinoc 33085.1 Sinocyclocheilus grahami|Golden-line barbel|XP 01609
Sinocyclocheilus anshuiensis|Barbel|XP 016310694.1
Labeo rohita|Rohu|RXN12068.1
Carassius auratus|Goldfish|XP 0261 rbel|XP 016145511.1 Carassius auratus|Goldfish|XP 026133081.1 ba
Poecilia formosa|Amazon molly|XP 016529234.1 Cyprinus carpio|Common carp|XP 018973259.1
Poecilia latipinna|Sailfin molly|XP 014902955.1 Sinocyclocheilusilus anshuiensis|Barbel|XP grahami|Golden-line 016296374.1 1 Poecilia mexicana|Shortfin molly|XP 014857406.1 Sinocycloche Gambusia affinis|Western mosquitofish|PWA30489.1 Xiphophorus couchianus|Monterrey flatyfish|XP 027900358.1 Sinocyclocheilus rhinocerous|Barbel|XP 016398918.1 Esox lucius|Northern pike|XP 019902374.1 Xiphophorus maculatus|Southern platyfish|XP 023181091.1 Esox lucius|Northern pike|XP 010902871.
Salvelinus alpinus|Arctic char|XP 023864507.1 Cyprinodon variegatus|Sheepshead minnow|XP 015256800.1 Poecilia reticulata|Guppy|XP 017160958.1 Salmo salar|Atlantic salmon|XP 014014782.1 Fundulus heteroclitus|Mummichog killifish|XP 012726913.1 Salmo trutta|Brown trout|XP 029570035.1 Kryptolebias marmoratus|Mangrove killifish|XP 024859480.1 Oncorhynchus kisutch|Coho salmon|XP 020308885.1
Austrofundulus limnaeus|Aplocheilidan fish|XP 013859069.1 Oncorhynchus nerka|Sockeye salmon|XP 029485639.1
Nothobranchius furzeri|Turquoise killifish|XP 015819926.1 Oncorhynchus mykiss|Rainbow trout|XP 021421236.1
Oryzias melastigma|Marine medaka ricefish|XP 024137991.1 Gadus morhua|Atlantic cod|XP 030196318.1
Myripristis murdjan|Pinecone soldierfish|XP 029933692.1 Oryzias latipes|Japanese ricefish|XP 023811560.1 Hippocampus comes|Tiger tail seahourse|XP 019739004.1
Oryzias javanicus|Javanese ricefish|RVE71825.1 Boleophthalmus pectinirostris|Great blue spotted mudskipper|XP 020779831.1 Parambassis ranga|Indian glassy fish|XP 028286263.1 Haplochromis burtoni|Burton s mouthbrooder fish|XP 005928876.2 Sphaeramia orbicularis|Orbiculate cardinalfish|XP 030019179.1
Lates calcarifer|Barramundi|XP 018533854.1
Pundamilia nyererei|Victorian cichlid|XP 005730063.2 Echeneis naucrates|Sharksucker remora|XP 029382885.1 Astatotilapia calliptera|Eastern river bream|XP 026031677.1 Seriola lalandi|Yellowtail amberjack|dorsalis XP 023267130.1
Seriola dumerili|Greater amberjack|XP 022598322.1 Maylandia zebra|Zebra mbuna|XP 012775099.1 |AWP17619.1 Neolamprologus brichardi|Princess cichlid|XP 006789368.1 Cynoglossus semilaevis|Tongue sole|XP 008312904.2
Scophthalmus maximus|Turbot Oreochromis niloticus|Nile talapia|XP 025764565.1 Paralichthys olivaceus|Olive flounder|XP 019962618.1
Perca flavescens|Yellow perch|TDH09587.1 Monopterus albus|Asian swamp eel|XP 020461959.1 Perca flavescens|Yellow perch|XP 028441029.1 Mastacembelus armatus|Zig-zag eel|XP 026171668.1
Cottoperca gobio|Thornfish|XP 029290260.1 Betta splendens|Betta fish|XP 028991038.1 Notothenia coriiceps|Black rockcod|XP 010778714.1 Anabas testudineus|Climbing perch|XP 026207186.1
Collichthys lucidus|Big head croaker|TKS73472.1 Tetraodon nigroviridis|Green spotted pufferfish|CAG07574.1 Larimichthys crocea|Large yellow croaker|XP 019118168.2 Takifugu rubripes|Japanese pufferfish|XP 011601522.1
Labrus bergylta|Ballan wrasse|XP 029132746.1 Takifugu flavidus|Yellowbelly pufferfish|TWW81346.1
Takifugu rubripes|Japanese pufferfish|XP 003967605.1 Takifugu bimaculatus|Futatsuboshi-fugu pufferfish|TNN03919.1
Takifugu rubripes|Japanese pufferfish|XP 029697935.1 Dicentrarchus labrax|European seabass|CBN81358.1
Takifugu flavidus|Yellowbelly pufferfish|TWW75703.1 Collichthys lucidus|Big head croaker|TKS89888.1
Takifugu bimaculatus|Futatsuboshi-fugu pufferfish|TNN00475.1 Larimichthys crocea|Large yellow croaker|XP 010749717.2
Tetraodon nigroviridis|Green spotted pufferfish|CAF98320.1 Perca flavescens|Yellow perch|XP 028424119.1 Acanthochromis polyacanthus|Spiny chromis damselfish|XP 022045252.1 Perca flavescens|Yellow perch|TDG98100.1
Labrus bergylta|Ballan wrasse|XP 020496309.1 Stegastes partitus|Bicolor damselfish|XP 008275071.1 Notothenia coriiceps|Black rockcod|XP 010773399.1 Amphiprion ocellaris|Ocellaris clownfish|XP 023145137.1 Cottoperca gobio|Thornfish|XP 029312630.1 Hippocampus comes|Tiger tail seahourse|XP 019731463.1 Gouania willdenowi|Blunt-snouted clingfish|XP 028331624.1 Monopterus albus|Asian swamp eel|XP 020475059.1 Acanthochromis polyacanthus|Spiny chromis damselfish|XP 022075405.1
Betta splendens|Betta fish|XP 029009541.1 Stegastes partitus|Bicolor damselfish|XP 008281509.1 Sphaeramia orbicularis|Orbiculate cardinalfish|XP 029992941.1 Amphiprion ocellaris|Ocellaris clownfish|XP 023149530.1 Boleophthalmus pectinirostris|Great blue spotted mudskipper|XP 020773191.1 Parambassis ranga|Indian glassy fish|XP 028286858.1
Lates calcarifer|Barramundi|XP 018535540.1 Oryzias latipes|Japanese ricefish|XP 011486369.1
Echeneis naucrates|Sharksucker remora|XP 029378315.1 Oryzias melastigma|Marine medaka ricefish|XP 024140646.1
Paralichthys olivaceus|Olive flounder|XP 019934578.1 Oryzias javanicus|Javanese ricefish|RVE59586.1 Nothobranchius furzeri|Turquoise killifish|XP 015818777.1 Scophthalmus maximus|Turbot|AWP08429.1 Nothobranchius furzeri|Turquoise killifish|XP 015818776.1 Seriola dumerili|Greater amberjack|XP 022610018.1 Kryptolebias marmoratus|Mangrove killifish|XP 017273956.2 Gouania willdenowi|Blunt-snouted clingfish|XP 028304125.1 Austrofundulus limnaeus|Aplocheilidan fish|XP 013863756.1 Gadus morhua|Atlantic cod|XP 030221252.1 Austrofundulus limnaeus|Aplocheilidan fish|XP 013863754.1 Myripristis murdjan|Pinecone soldierfish|XP 029909817.1 Fundulus heteroclitus|Mummichog killifish|XP 021172442.1 Oncorhynchus kisutch|Coho salmon|XP 020311565.1 Cyprinodon variegatus|Sheepshead minnow|XP 015249777.1 Oncorhynchus mykiss|Rainbow trou t|XP 021436569. 1 Gambus ia affinis |We s te rn mos quitofis h|PWA 16728.1
Oncorhynchus mykiss|Rainbow trout|CDQ85740.1 Xiphophorus maculatus|Southern platyfish|XP 014330290.1
Salmo salar|Atlantic salmon|XP 013981607.1 Xiphophorus couchianus|Monterrey flatyfish|XP 027891969.1
Esox lucius|Northern pike|XP 028971064.1 Poecilia reticulata|Guppy|XP 008435047.1
Cyprinus carpio|Common carp|KTG33728.1 Poecilia latipinna|Sailfin molly|XP 014877372.1
Cyprinus carpio|Common carp|XP 018920439.1 Poecilia mexicana|Shortfin molly|XP 014829011.1
Danionella translucida|Glassfish|TRY94604.1 Poecilia formosa|Amazon molly|XP 016520150.1 Oreochromis niloticus|Nile talapia|XP 005448371.1 Sinocyclocheilus grahami|Golden-line barbel|XP 016145822.1 Neolamprologus brichardi|Princess cichlid|XP 006805836.1 Sinocyclocheilus rhinocerous|Barbel|XP 016377373.1 Pundamilia nyererei|Victorian cichlid|XP 005748575.1 Carassius auratus|Goldfish|XP 026058554.1 Astatotilapia calliptera|Eastern river bream|XP 026018622.1 Sinocyclocheilus anshuiensis|Barbel|XP 016354555.1 Maylandia zebra|Zebra mbuna|XP 004571460.2 Sinocyclocheilus grahami|Golden-line barbel|XP 016086936.1 Astatotilapia calliptera|Eastern river bream|XP 026018621.1 Cyprinus carpio|Common carp|KTF85211.1 Oreochromis niloticus|Nile talapia|XP 019218187.1 Carassius auratus|Goldfish|XP 026094973.1 Neolamprologus brichardi|Princess cichlid|XP 006805839.1 Bagarius yarrelli|Giant devil catfish|TSM52293.1 Maylandia zebra|Zebra mbuna|XP 004571458.2
Tachysurus fulvidraco|Yellowhead catfish|XP 027029194.1 Astatotilapia calliptera|Eastern river bream|XP 026018624.1
Ictalurus punctatus|Freshwater catfish|XP 017340266.1 Haplochromis burtoni|Burton s mouthbrooder fish|XP 014193347.1
Astyanax mexicanus|Blind cave fish|XP 015460620.2 Pundamilia nyererei|Victorian cichlid|XP 005748581.1
Oreochromis niloticus|Nile talapia|XP 019218186.1 Pygocentrus nattereri|Red-bellied piranha|XP 017563833.1 Astatotilapia calliptera|Eastern river bream|XP 026018623.1 Electrophorus electricus|Electric eel|XP 026858998.1 Neolamprologus brichardi|Princess cichlid|XP 006805838.1 Denticeps clupeoides|Denticle herring|XP 028814301.1 Maylandia zebra|Zebra mbuna|XP 024659218.1 Clupea harengus|Atlantic herring|XP 012674098.1 Oreochromis niloticus|Nile talapia|XP 019218227.1 Poecilia latipinna|Sailfin molly|XP 014877902.1 Neolamprologus brichardi|Princess cichlid|XP 006805837.1 Poecilia formosa|Amazon molly|XP 007540581.2 Maylandia zebra|Zebra mbuna|XP 004571459.2
Xiphophorus couchianus|Monterrey flatyfish|XP 027869861.1 Astatotilapia calliptera|Eastern river bream|XP 026018696.1 Poecilia mexicana|Shortfin molly|XP 014835088.1 Haplochromis burtoni|Burton s mouthbrooder fish|XP 005952892.2 Poecilia reticulata|Guppy|XP 008400926.1 1 Pundamilia nyererei|Victorian cichlid|XP 005748573.1 Gambusia affinis|Western mosquitofish|PWA19871.1 Pundamilia nyererei|Victorian cichlid|XP 005748574.1
Aurelia|Moon jelly|Seg1803.3 transcript id Seg1803.3|GoldUCD|mRNA.D3Y31 Cyprinodon variegatus|Sheepshead minnow|XP 015248324.1 Hofstenia miamia|Three-banded panther worm|scaffold112 437721 469571 R Fundulus heteroclitus|Mummichog killifish|XP 021167590.1 Hofstenia miamia|Three-banded panther worm|scaffold18 807971 810976 F Fundulus heteroclitus|Mummichog killifish|XP 012715801.2 Hofstenia miamia|Three-banded panther worm|scaffold18 866412 869501 F Nothobranchius furzeri|Turquoise killifish|XP 015808722.1 Praesagittifera naikaiensis|Acoel flatworm|g36760.t1 Austrofundulus limnaeus|Aplocheilidan fish|XP 013883983.1 Oryzias latipes|Japanese ricefish|XP 011485404.1 Praesagittifera naikaiensis|Acoel flatworm|g11490.t1 Praesagittifera naikaiensis|Acoel flatworm|g4935.t1 Parambassis ranga|Indian glassy fish|XP 028257133.1 Praesagittifera naikaiensis|Acoel flatworm|g16021.t1 Pundamilia nyererei|Victorian cichlid|XP 013764590.1 Hofstenia miamia|Three-banded panther worm|scaffold488 5372 8923 F
Hofstenia miamia|Three-banded panther worm|scaffold387 196851 200279 F Haplochromis burtoni|Burton s mouthbrooder fish|XP 014191295.1 Astatotilapia calliptera|Eastern river bream|XP 026031739.1 Hofstenia miamia|Three-banded panther worm|scaffold387 254022 257456 F Maylandia zebra|Zebra mbuna|XP 004539903.1 Hofstenia miamia|Three-banded panther worm|scaffold387 247539 251000 F Maylandia zebra|Zebra mbuna|XP 024658395.1 Hofstenia miamia|Three-banded panther worm|scaffold387 219858 223314 F
Oreochromis niloticus|Nile talapia|XP 019216783.1 Hofstenia miamia|Three-banded panther worm|scaffold391 331241 359300 R Perca flavescens|Yellow perch|TDH17505.1 Hofstenia miamia|Three-banded panther worm|scaffold387 211324 251000 F Perca flavescens|Yellow perch|XP 028437026.1 Hydra vulgaris|Fresh-water polyp|XP 012554388 Cottoperca gobio|Thornfish|XP 029283252.1 Hydra vulgaris|Fresh-water polyp|
Labrus bergylta|Ballan wrasse|XP 020512138.1 Hydra vulgaris|Fresh-water polyp|XP 012561335
Aiptasia|Sea anemone|AIPGENE12924 XP 012562662 Larimichthys crocea|Large yellow croaker|XP 019118536.1 Nematostella vectensis|Starlet sea anemone|Nemve1|85952
Pocillo Takifugu bimaculatus|Futatsuboshi-fugu pufferfish|TNM90537.1 pora Takifugu rubripes|Japanese pufferfish|XP 029701936.1 da Goniastrea aspera|Stonymicornis|Lace coral|gasp1.m3.26670.m1 Takifugu flavidus|Yellowbelly pufferfish|TWW67542.1 Fungia spp.|Coral|ffun1.m4.18855.m1 coral|pdam Aiptasia|Sea anemone|AIPGENE19094 Tetraodon nigroviridis|Green spotted pufferfish|CAG00817.1 00014050-RA Nematostella vectensis|Starlet sea anemone|Nemve1|102167 Gouania willdenowi|Blunt-snouted clingfish|XP 028330305.1 Porites lutea|Small polyp stony coral|plut2.m8.21682.m1 Hippocampus comes|Tiger tail seahourse|XP 019748829.1 Pocillopora damicornis|Lace coral|pdam 00020220-RA Amphiprion ocellaris|Ocellaris clownfish|XP 023125132.1 Anabas testudineus|Climbing perch|XP 026234483.1 Goniastrea aspera|Stony coral|gasp1.m3.6037.m1 Betta splendens|Betta fish|XP 029000564.1 Fungia spp.|Coral|ffun1.m4.18290.m1
Praesagittifera naikaiens Mastacembelus armatus|Zig-zag eel|XP 026157016.1 Praesagittifera naikaiensis|Acoel flatworm|g25700.t1 ParalichthysScophthalmus olivaceus|Olive maximus|Turbot|AWP01083.1 flounder|XP 019965983.1 Drosophila melanogaster|Common fruit fly|Trpm is|Acoel flatworm|g40739. Ciona intestinalis|Vase tunicate|XP 018667443 Cynoglossus semilaevis|Tongue sole|XP 016888202.1 Branchiostoma lance Seriola dumerili|Greater amberjack|XP 022605701.1 Branchiostoma belcheri|Lancelet|XP 019640450 t1
Petromyzon marinus|Seaolatum|European lamprey|PMZ 0026066-RA lacelet|BL Seriola lalandi|Yellowtail amberjack|dorsalis XP 023278617.1 Callorhinchus milii|Ghostshark|XP 007903830.1 Echeneis naucrates|SharksuckerLates calcarifer|Barramundi|XP remora|XP 029353277.1 018548296.1 Chiloscyllium punctatum|Brownbanded bamboo shark|Chipu0017399.p1
Rhincodon typus|Whale shark|XP 020370945.1 24111 ev m0 Sphaeramia orbicularis|Orbiculate cardinalfish|XP 030019129.1 Carcharodon car Gadus morhua|Atlantic cod|XP 030232951.1 Scyliorhinus torazame|Cloudy catshark|Scyto0018498.p1
Latimeria chalumnae|Coelacanth|XPcharias|Great white 005989708.1 shark|ScRDnwc 11009 118 Salmo salar|Atlantic salmon|XP 014042399.1 Boleophthalmus pectinirostris|GreatMyripristis bluemurdjan|Pinecone spotted mudskipper|XP soldierfish|XP 020790203.1 029937440.1 Gallus gallus|Chicken|TRPM7 E1BYV3 CHICK
Salmo salar|Atlantic salmon|XP 014042415.1 Mus musculus|Mouse|Trpm7 CC Salmo trutta|Brown trout|XP 029624208.1 Homo sapiens|Human|TRPM7 CCDS42035.1 Salmo trutta|Brown trout|XP 029600751.1 Erpetoichthys calabaricus|Freshwater snakefish|XP 028679241.1 Esox lucius|Northern pike|XP 019912150.1 Lepisosteus oculatus|Spotted gar|XP 006628750.2 6063 Scleropages formosus|Asian arowana|XP 018620561.2DS16689.1 -snap-ge n Paramormyrops kingsleyae|Elephantfish|XP 02367287 e-4.2-mRNA-1
SinocyclocheilusSinocyclocheilus grahami|Golden-line anshuiensis|Barbel|XP barbel|XP 016128645.1 016323877.1 Paramormyrops kingsleyae|Elephantfish|XP 023669829.1
Scleropages SinocyclocheilusCarassius rhinocerous|Barbel|XP auratus|Goldfish|XP 016421415.1 026067692.1 Scleropages formosus|Asian arowana|KPP79945.1 Cyprinus carpio|Common carp|KTG31988.1 for Denticeps clupeoides|Denticlemosus| herring|XP 028811577.1 Carassius auratus|Goldfish|XP 026072524.1 Clupea harengus|Atlantic herring|XPAsian arowana|XP012670305.1 018619235.1
Cyprinus carpio|Common carp|XP 018961964.1 Astyanax mexicanus|Blind cave fish|XP 022542764.1
Electrophorus electricus|Electric eel|XP 026878579.1 7 .1 Danionella translucida|Glassfish|TRY90810.1 Pygocentrus nattereri|Red-bellied piranha|XP 017541300.1
Sinocyclocheilus grahami|Golden-line barbel|XP 016138202.1 Pangasianodon hypophthalmus|Irridescent shark catfish|XP 026769698.1
Astyanax mexicanus|Blind cave fish|XP 022523105.1 Ictalurus punctatus|Fres
Anabarilius grahami|Kanglang fish|ROL42174.1
Pygocentrus nattereri|Red-bellied piranha|XP 017577051.1 Cyprinus carpio|Common carp|KTF80090.1
ParamormyropsScleropages kingsleyae|Elephantfish|XP formosus|Asian arowana|KPP69568.1 023672886.1 Carassius auratus|Goldfish|XP 026086140.1 hwa Lepisosteus oculatus|Spotted gar|XP 006629107.1 Carassius auratus|Goldfi ter catfish|XP 017321526.1 Danionella translucida|Glassfis Homo sapiens|Human|TRPM1 CCDS10024.2 Cyprinus carpio|Common carp|XP 018918379.1 Mus musculus|Mouse|TRPM1 CCDS21332.2 Sinocyclocheilus grahami|Golden-l Erpetoichthys calabaricus|Freshwater snakefish|XP 028679601.1 Gallus gallus|Chicken|TRPM1 F1NCK1 CHICK Sinocyclocheilus anshuiensis|Barbel|XPsh 016362233.1 |XP 026142699.1 Sinocyclocheilus anshuiensis|Barbel|XP 016343860.1 Latimeria chalumnae|Coelacanth|XP 014348116.1 h|TR Rhincodon typus|Whale shark|XP 020377793.1 Sinocyclocheilus rhinocerous|Barbel|XP 016388268.1 Z01995.1 Esox lucius|Northern pike|XP 028970005.1
Esox lucius|Northern pike|XP 028980331.1
Salmo trutta|Brown trout|XP 029623819.1 ine barbel Callorhinchus milii|Ghostshark|XP 007907039.1 Salmo trutta|Brown trout|XP 029576820.1 |XP 016111632.1 Salmo salar|Atlantic salmon|XP 014031727.1 Chiloscyllium punctatum|Brownbanded bamboo shark|Chipu0014745.pl Oncorhynchus nerka|Sockeye salmon|XP 029510198.1
Oncorhynchus mykiss|Rainbow trout|XP 021463470.1
Kryptolebias marmoratus|Mangrove killifish|XP 024861897.1 Oncorhynchus tshawytscha|Chinook salmon|XP 024275007.1 Eptatretus burgeri|Inshore hagfish|trpm1b-201|ENSEBUP00000012388 Oncorhynchus mykiss|Rainbow trout|CDQ95902.1 Kryptolebias marmoratus|Mangrove killifish|XP 017272693.1 Gadus morhua|Atlantic cod|XP 030232224.1 Carcharodon carcharias|Great white shark|ScRDnwcAustrofundulus 41824 761978-snap-gene-2.12-mRNA-1 limnaeus|Aplocheilidan fish|XP 013867998.1 Nothobranchius furzeri|Turquoise killifish|XP 015827870.1 Myripristis murdjan|Pinecone soldierfish|XP 029935152.1 Oryzias latipes|Japanese ricefish|XP 011477606.1 Sphaeramia orbicularis|Orbiculate cardinalfish|XP 030019380.1
Cyprinodon variegatus|SheepsheadOryzias latipes|Japanese minnow|XP ricefish|XP 015254410.1 020561681.1 Hippocampus comes|Tiger tail seahourse|XP 019722157.1
Oryzias javanicus|Javanese ricefish|RVE68416.1 Cottoperca gobio|Thornfish|XP 029311396.1
Perca flavescens|Yellow perch|XP 028441368.1
Tetraodon nigroviridis|Green spotted pufferfish|CAG00828.1
Takifugu rubripes|Japanese pufferfish|XP 029702159.1 Oryzias melastigma|Marine medaka ricefish|XP 024130907.1 Amphiprion ocellaris|Ocellaris clownfish|XP 023129489.1 Takifugu flavidus|Yellowbelly pufferfish|TWW67509.1
Labrus bergylta|Ballan wrasse|XP 029132737.1 Stegastes partitus|Bicolor damselfish|XP 008286577.1 Collichthys lucidus|Big head croaker|TKS69858.1
Parambassis ranga|Indian glassy fish|XP 028269151.1 Larimichthys crocea|Large yellow croaker|XP 019134437.1
Pundamilia nyererei|Victorian cichlid|XP 013765385.1 Larimichthys crocea|Large yellow croaker|TMS13153.1 Acanthochromis polyacanthus|Spiny chromis damselfish|XP 022056549.1 Boleophthalmus pectinirostris|Great blue spotted mudskipper|XP 020797363.1
Maylandia zebra|Zebra mbuna|XP 004544399.1 Cynoglossus semilaevis|Tongue sole|XP 024911175.1 Lates calcarifer|Barramundi|XP 018522231.1 Astatotilapia calliptera|Eastern river bream|XP 026042875.1 Haplochromis burtoni|Burton s mouthbrooder fish|XP 014189162.1 Paralichthys olivaceus|Olive flounder|XP 019935318.1
Oreochromis niloticus|Nile talapia|XP 025752804.1 Scophthalmus maximus|Turbot|AWP01851.1 Neolamprologus brichardi|Princess cichlid|XP 006784836.1 Echeneis naucrates|Sharksucker remora|XP 029353347.1 Salarias fasciatus|Jewelled blenny|XP 029961657.1 Seriola lalandi|Yellowtail amberjack|dorsalis XP 023267720.1
Takifugu flavidus|Yellowbelly pufferfish|TWW59976.1 Seriola dumerili|Greater amberjack|XP 022596044.1
Betta splendens|Betta fish|XP 029000985.1
Labrus bergylta|Ballan wrasse|XP 020512953.1 Anabas testudineus|Climbing perch|XP 026234732.1 TetraodonTakifugu nigroviridis|Green rubripes|Japanese spotted pufferfish|CAF91479.1pufferfish|XP 029685688.1 Mastacembelus armatus|Zig-zag eel|XP 026149008.1 Poecilia formosa|Amazon molly|XP 016532616.1 Mastacembelus armatus|Zig-zag eel|XP 026149005.1 Poecilia reticulata|Guppy|XP 017162228.1 Poecilia formosa|Amazon molly|XP 007563084.1 Monopterus albus|Asian swamp eel|XP 020475587.1
Monopterus albus|Asian swamp eel|XP 020448136.1
Gouania willdenowi|Blunt-snouted clingfish|XP 028298890.1
Salarias fasciatus|Jewelled blenny|XP 029956408.1 Poecilia mexicana|Shortfin molly|XP 014859528.1 Poecilia latipinna|Sailfin molly|XP 014880463.1 Stegastes partitus|Bicolor damselfish|XP 008304235.1
Xiphophorus maculatus|Southern platyfish|XP 023199739.1 Amphiprion ocellaris|Ocellaris clownfish|XP 023125898.1 Acanthochromis polyacanthus|Spiny chromis damselfish|XP 022055239.1
Gambusia affinis|Western mosquitofish|PWA19557.1 Parambassis ranga|Indian glassy fish|XP 028258647.1
Oryzias latipes|Japanese ricefish|XP 020555660.1
Oryzias melastigma|Marine medaka ricefish|XP 024151070.1
Perca flavescens|Yellow perch|TDH13153.1 Oryzias javanicus|Javanese ricefish|RVE74735.1 Xiphophorus couchianus|Monterrey flatyfish|XP 027888550.1 Fundulus heteroclitus|Mummichog killifish|XP 012718782.1 Oreochromis niloticus|Nile talapia|XP 025763103.1 Neolamprologus brichardi|Princess cichlid|XP 006779896.1 Fundulus heteroclitus|MummichogPerca killifish|XPflavescens|Yellow 021172603.1 perch|XP 028434844.1 Cottoperca gobio|Thornfish|XP 029296038.1 Haplochromis burtoni|Burton s mouthbrooder fish|XP 014188582.1
Gouania willdenowi|Blunt-snouted clingfish|XP 028314174.1 Pundamilia nyererei|Victorian cichlid|XP 013770320.1
Astatotilapia calliptera|Eastern river bream|XP 026028237.1
Maylandia zebra|Zebra mbuna|XP 012775834.1 Notothenia coriiceps|Black rockcod|XP 010765830.1 Maylandia zebra|Zebra mbuna|XP 004539660.1
Collichthys lucidus|Big head croaker|TKS78914.1 Nothobranchius furzeri|Turquoise killifish|XP 015797213.1
Kryptolebias marmoratus|Mangrove killifish|XP 017274221.1
Larimichthys crocea|Large yellow croaker|XP 019131520.1 Austrofundulus limnaeus|Aplocheilidan fish|XP 013865887.1
Betta splendens|Betta fish|XP 029016484.1 Fundulus heteroclitus|Mummichog killifish|XP 021165359.1 Mastacembelus armatus|Zig-zag eel|XP 026177102.1 Cyprinodon variegatus|Sheepshead minnow|XP 015253027.1 Hippocampus comes|Tiger tail seahourse|XP 019741177.1 Monopterus albus|Asian swamp eel|XP 020449951.1 Gambusia affinis|Western mosquitofish|PWA18942.1
Xiphophorus maculatus|Southern platyfish|XP 023188329.1
Xiphophorus couchianus|Monterrey flatyfish|XP 027871107.1 Anabas testudineus|Climbing perch|XP 026198504.1 Poecilia reticulata|Guppy|XP 017159533.1
Poecilia latipinna|Sailfin molly|XP 014898269.1 Scophthalmus maximus|Turbot|AWP06873.1 Poecilia mexicana|Shortfin molly|XP 014840617.1 Seriola dumerili|Greater amberjack|XP 022607229.1 Poecilia formosa|Amazon molly|XP 016525472.1
Callorhinchus milii|Ghostshark|XP 007897564.1 Seriola lalandi|Yellowtail amberjack|dorsalis XP 023274054.1Lates calcarifer|Barramundi|XP 018524646.1 Scyliorhinus torazame|Cloudy catshark|Scyto0003223.p1 Echeneis naucrates|Sharksucker remora|XP 029367516.1 Chiloscyllium punctatum|Brownbanded bamboo shark|Chipu0003513.p1 Paralichthys olivaceus|Olive flounder|XP 019949432.1 Latimeria chalumnae|Coelacanth|XP 014349563.1 7 Gallus gallus|Chicken|TRPM6 E1C5L2 CHICK
Mus musculus|Mouse|Trpm6 CCDS29691.1
Homo sapiens|Human|TRPM6 CCDS6647.1
Acipenser ruthenus|Sterlet sturgeon|RXM98791.1 Salmo trutta|Brown trout|XP 029618228.1 Erpetoichthys calabaricus|Freshwater snakefish|XP 028658188.1
Lepisosteus oculatus|Spotted gar|XP 015222636.1 Oncorhynchus kisutch|Coho salmon|XP 020344126.1 Sphaeramia orbicularis|OrbiculateMyripristis murdjan|Pinecone cardinalfish|XP soldierfish|XP029998899.1Salmo 029915962.1 salar|Atlantic salmon|XP 014016106.1 Paramormyrops kingsleyae|Elephantfish|XP 023659018.1 Oncorhynchus mykiss|Rainbow trout|XP 021476775.1 Scleropages formosus|Asian arowana|XP 029115373.1 Scleropages formosus|Asian arowana|KPP76789.1
Denticeps clupeoides|Denticle herring|XP 028852074.1
Clupea harengus|Atlantic herring|XP 012685821.1
Salvelinus alpinus|Arctic char|XP 023834998.1 Electrophorus electricus|Electric eel|XP 026883697.1 Oncorhynchus nerka|Sockeye salmon|XP 029533489.1 Pygocentrus nattereri|Red-bellied piranha|XP 017572185.1 Salmo trutta|Brown trout|XP 029573444.1 Astyanax mexicanus|Blind cave fish|XP 022523984.1
Pangasianodon hypophthalmus|Irridescent shark catfish|XP 026795426.1
Tachysurus fulvidraco|Yellowhead catfish|XP 026996995.1 Boleophthalmus pectinirostris|Great blue spotted mudskipper|XP 020791221.1 Salmo salar|Atlantic salmon|XP 014026928.1 Ictalurus punctatus|Freshwater catfish|XP 017307824.1 Oncorhynchus mykiss|Rainbow trout|XP 021461164.1 Ictalurus punctatus|Freshwater catfish|XP 017307822.1 Oncorhynchus tshawytscha|Chinook salmon|XP 024296470.1 Danionella translucida|Glassfish|TRY81916.1 Oncorhynchus nerka|Sockeye salmon|XP 029493231.1 Esox lucius|Northern pike|XP 019908473.1 Anabarilius grahami|Kanglang fish|ROI27737.1
Salvelinus alpinus|Arctic char|XP 023859010.1 Cyprinus carpio|Common carp|XP 018954100.1
Carassius auratus|Goldfish|XP 026068374.1 Gadus morhua|Atlantic cod|XP 030214469.1 Sinocyclocheilus grahami|Golden-line barbel|XP 016147135.1 Oncorhynchus kisutch|Coho salmon|XP 020318034.1 Sinocyclocheilus rhinocerous|Barbel|XP 016413626.1 Labeo rohita|Rohu|RXN32075.1 Sinocyclocheilus anshuiensis|Barbel|XP 016322474.1
Cyprinus carpio|Common carp|XP 018952131.1
Cyprinus carpio|Common carp|KTF84153.1 Sinocyclocheilus anshuiensis|Barbel|XP 016321494.1
Sinocyclocheilus grahami|Golden-line barbel|XP 016137272.1
Sinocyclocheilus rhinocerous|Barbel|XP 016397097.1 Sinocyclocheilus rhinocerous|Barbel|XP 016423984.1 Esox lucius|Northern pike|XP 010875152.3
Salvelinus alpinus|Arctic char|XP 023834790.1
Salmo trutta|Brown trout|XP 029617770.1
Salmo salar|Atlantic salmon|XP 014016160.1 Sinocyclocheilus anshuiensis|Barbel|XP 016321594.1 Oncorhynchus mykiss|Rainbow trout|XP 021475589.1
Oncorhynchus nerka|Sockeye salmon|XP 029534850.1 Sinocyclocheilus rhinocerous|Barbel|XP 016413553.1 Danionella translucida|Glassfish|TRY55609.1 Oncorhynchus tshawytscha|Chinook salmon|XP 024296486.1 Carassius auratus|Goldfish|XP 026068400.1 Oncorhynchus kisutch|Coho salmon|XP 020345185.1 Salmo trutta|Brown trout|XP 029573485.1 Cyprinus carpio|Common carp|KTF94157.1 Salmo salar|Atlantic salmon|XP 014027690.1 Sinocyclocheilus grahami|Golden-line barbel|XP 016137288.1 Sinocyclocheilus anshuiensis|Barbel|XP 016322473.1 Salvelinus alpinus|Arctic char|XP 023859073.1 Oncorhynchus mykiss|Rainbow trout|XP 021461144.1
Oncorhynchus nerka|Sockeye salmon|XP 029493209.1
Oncorhynchus tshawytscha|Chinook salmon|XP 024272767.1 Carassius auratus|Goldfish|XP 026112525.1 Oncorhynchus kisutch|Coho salmon|XP 020324767.1 Myripristis murdjan|Pinecone soldierfish|XP 029915109.1
Boleophthalmus pectinirostris|Great blue spotted mudskipper|XP 020791136.1
Cyprinus carpio|Common carp|XP 018978129.1 Sphaeramia orbicularis|Orbiculate cardinalfish|XP 029999816.1 Lates calcarifer|Barramundi|XP 018524238.1
Sinocyclocheilus grahami|Golden-line barbel|XP 016140787.1 Scophthalmus maximus|Turbot|AWP06776.1 Paralichthys olivaceus|Olive flounder|XP 019949511.1
Cynoglossus semilaevis|Tongue sole|XP 016898711.1 Cynoglossus semilaevis|Tongue sole|XP 016898707.1 Cyprinus carpio|Common carp|XP 018950380.1 Echeneis naucrates|Sharksucker remora|XP 029365578.1 Seriola lalandi|Yellowtail amberjack|dorsalis XP 023249760.1
Seriola dumerili|Greater amberjack|XP 022611056.1 Betta splendens|Betta fish|XP 029019883.1 Anabas testudineus|Climbing perch|XP 026198544.1
Mastacembelus armatus|Zig-zag eel|XP 026176431.1
Mastacembelus armatus|Zig-zag eel|XP 026176428.1
Monopterus albus|Asian swamp eel|XP 020446050.1 Monopterus albus|Asian swamp eel|XP 020446048.1
Tetraodon nigroviridis|Green spotted pufferfish|CAF92603.1
Labrusbergylta|Ballan wrasse|XP 020488652.1 Hippocampus comes|Tiger tail seahourse|XP 019717802.1
Larimichthys crocea|Large yellow croaker|XP 027133495.1 Takifugu rubripes|Japanese pufferfish|XP 029685461.1
Collichthys lucidus|Big head croaker|TKS79008.1
Bagarius yarrelli|Giant devil catfish|TSR04284.1
Ictalurus punctatus|Freshwater catfish|XP 017307289.1
Tachysurus fulvidraco|Yellowhead catfish|XP 026997302.1 Electrophorus electricus|Electric eel|XP 026875675.1
Clupea harengus|Atlantic herring|XP 012685828.1 Astyanax mexicanus|Blind cave fish|XP 022521978.1
Scleropages formosus|Asian arowana|KPP65346.1 Homo sapiens|Human|TRPM3 CCDS6634.1
Mus musculus|Mouse|Trpm3 CCDS29702.1 Pygocentrus nattereri|Red-bellied piranha|XP 017565139.1 Denticeps clupeoides|Denticle herring|XP 028852652.1 Gallus gallus|Chicken|TRPM3 F1NJZ7 CHICK Acipenser ruthenus|Sterlet sturgeon|RXM98925.1
Scleropages formosus|Asian arowana|XP 018615585.1
Callorhinchus milii|Ghostshark|XP 007904772.1 Paramormyrops kingsleyae|Elephantfish|XP 023659048.1 Poecilia reticulata|Guppy|XP 008417358.1 Pangasianodon hypophthalmus|Irridescent shark catfish|XP 026795585.1 Latimeria chalumnae|Coelacanth|XP 014347481.1 Poecilia reticulata|Guppy|XP 017162093.1
Poecilia formosa|Amazon molly|XP 007564533.1
Poecilia latipinna|Sailfin molly|XP 014892804.1
Poecilia mexicana|Shortfin molly|XP 014826160.1
Erpetoichthys calabaricus|Freshwater snakefish|XP 028658171.1
Gambusia affinis|Western mosquitofish|PWA19620.1
Oryzias javanicus|Javanese ricefish|RVE68490.1
Perca flavescens|Yellow perch|TDH13356.1
Oryzias latipes|Japanese ricefish|XP 020561734.1
Cottoperca gobio|Thornfish|XP 029294787.1
Xiphophorus maculatus|Southern platyfish|XP 014327969.1 Xiphophorus couchianus|Monterrey flatyfish|XP 027890609.1 Maylandia zebra|Zebra mbuna|XP 004544277.1
3 Perca flavescens|Yellow perch|XP 028435015.1 Fundulus heteroclitus|Mummichog killifish|XP 021163609.1
Cyprinodon variegatus|Sheepshead minnow|XP 015242881.1 Austrofundulus limnaeus|Aplocheilidan fish|XP 013867494.1 Nothobranchius furzeri|Turquoise killifish|XP 015827713.1 Oreochromis niloticus|Nile talapia|XP 005473206.1
Chiloscyllium punctatum|Brownbanded bamboo shark|Chipu0016510.p1 Austrofundulus limnaeus|Aplocheilidan fish|XP 013867499.1 Stegastes partitus|Bicolor damselfish|XP 008293932.1 Pundamilia nyererei|Victorian cichlid|XP 005729535.1 Parambassis ranga|Indian glassy fish|XP 028268996.1 Salarias fasciatus|Jewelled blenny|XP 029960285.1 Amphiprion ocellaris|Ocellaris clownfish|XP 023146525.1
Kryptolebias marmoratus|Mangrove killifish|XP 024861877.1 Amphiprion ocellaris|Ocellaris clownfish|XP 023146527.1
Oryzias melastigma|Marine medaka ricefish|XP 024131712.1
Neolamprologus brichardi|Princess cichlid|XP 006805945.1
Astatotilapia calliptera|Eastern river bream|XP 026042277.1
Gouania willdenowi|Blunt-snouted clingfish|XP 028312763.1
Gouania willdenowi|Blunt-snouted clingfish|XP 028313884.1
Gouania willdenowi|Blunt-snouted clingfish|XP 028312840.1 Gouania willdenowi|Blunt-snouted clingfish|XP 028312746.1
Haplochromis burtoni|Burton s mouthbrooder fish|XP 005922832.1
Acanthochromis polyacanthus|Spiny chromis damselfish|XP 022058346.1 UFboot Support
95 100 6
Figure S17