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12Th ANNUAL WV-INBRE SUMMER RESEARCH SYMPOSIUM 12th ANNUAL WV-INBRE SUMMER RESEARCH SYMPOSIUM West Virginia IDeA Network for Biomedical Research Excellence (WV-INBRE) Participants JULY 29, 2013 Marshall University Huntington, WV Program and Abstract Book West Virginia IDeA Network of Biomedical Research Excellence (WV-INBRE) 12th Annual Summer Research Symposium Marshall University Huntington, WV July 29, 2013 Memorial Student Center – Room BE-5 8:00-9:00 AM: Registration and Breakfast 9:00–9:10 AM: Welcoming Remarks, Dr. Joseph Shapiro, Dean, Joan C. Edwards School of Medicine, Marshall University 9:10-9:15 AM: Opening Remarks, Dr. Gary Rankin, Principal Investigator, West Virginia IDeA Network of Biomedical Research Excellence Oral Presentations by WV-INBRE Participants 9:15–9:35 AM: Cara Halldin, Ph.D., Epidemiologist, CDC, NIOSH, Division of Respiratory Disease Studies, Morgantown, WV. "Genetic Sequencing of Malaria Vectors in the South Pacific: Phylogenetic Analysis and Xenomonitoring." 9:35-9:50 AM: Jessica Allen, Concord University, WV-INBRE Intern, “Investigating the Role of Vascular Endothelial Growth Factor in Adipocyte Specific VEGF Deficient Mice.” 9:50-10:05 AM: Rebecca Martin, Davis & Elkins College, WV-INBRE Intern, “The effects of TRPV6 in human small cell lung cancer cells” 10:05–10:20 AM: Kathy Loughman, John Marshall High School, Glen Dale, WV, WV-INBRE/HSTA Fellow, “The role of multidrug efflux pumps in the temperature-dependent gentamicin resistance of pathogenic bacteria.” 10:20–10:30 AM: Break 10:30–10:45 AM: Sricharan Mahavadi, Shepherd University, WV-INBRE Intern, “Metastatic Activity of Breast Cancer Cells in the Presence of NEDD9.” 10:45-11:00 AM: Jennifer Franko, Ph.D., Biology Department, Bethany College, Bethany, WV, WV-INBRE Summer Fellow, "The role of XX vs. XY sex chromosome complement in the regulation of innate immune responses" Keynote Speaker 11:00 AM–Noon: Brad Goodner, Ph.D., Professor of Biology, Edward J. Smerek Chair of Mathematics, the Sciences, & Technology; Director of the Center for Deciphering Life's Languages, Hiram College. "Beyond Your Textbook: Using Genomics and Bioinformatics to Test the Role of Model Organisms" Lunch Noon–1:00 PM Don Morris Room 1:00-2:00 PM Room BE-5 Don Primerano, Ph.D., Section Chief Microbiology, Department of Biochemistry and Microbiology, Genomics Core Director, JCE School of Medicine, Marshall University, and Jim Denvir, Ph.D., Assistant Professor, Department of Biochemistry and Microbiology, Bioinformatic and Statistical Analyst, JCE School of Medicine, Marshall University “Applications of Next Gen Sequencing in Genomics, Transcriptomics and Microbiome Studies” 1:00-2:00 pm Shawkey Dining Room (2E28) Diana Maue, Graduate Recruitment, and Communication Coordinator Biomedical Sciences and S.T.E.M. Disciplines, Marshall University “Applying to Graduate School” Poster Session 2:00–3:30 PM Don Morris Room "Beyond Your Textbook: Using Genomics and Bioinformatics to Test the Role of Model Organisms". Brad Goodner, Ph.D. Professor of Biology, Edward J. Smerek Chair of Mathematics, the Sciences, & Technology, and Director of the Center for Deciphering Life's Languages, Hiram College. I was born and raised in north-central Texas (apologies in advance for the accent), but I don't wear boots, a ten-gallon hat, or a belt with a big buckle. My academic history has included stints as an Aggie, a Boilermaker, a Tar Heel, a Spider, and now a Terrier (extra points if you can name all the schools). As a biologist, I have worked on a variety of topics over the years, but curiously all connected to the letter "b" - bacteria, birds, botany, bumblebees, and butterflies. At Hiram, I teach a variety of courses with most of them focused on very small things - single cells, biochemical pathways, proteins, and genes. As you know, most genes are segments of DNA that tell the cell "make a particular protein at a particular time and place." Over most of the last 50 years, scientists have studied genes and the proteins they encode one at a time. While this approach has been highly successful, there are questions that are best addressed by looking at many or all of the genes of an organism at the same time. All the genes of an organism are known collectively as its genome, and the study of genomes is called genomics. Genomics not only can answer questions about known genes, but the determination of the entire DNA sequence of an organism also exposes hundreds to thousands of "new" genes and just as many new questions for future research. My students and I use a variety of genetic and genomic tools to understand how "simple" bacteria do their thing. Specifically, we are interested in how bacteria organize their genes into a genome, and how they interact with their environment and with other organisms. We do this through incorporation of research into courses at Hiram College, independent research projects, and outreach to high schools both near and far away. Over the last several years, I have worked with others on some emerging national science education efforts. While I love to share what has worked and what has not worked for me, I freely admit that I am constantly on the lookout for great questions, ideas, and strategies that will move us to another level. Outside of academia, my life revolves around the 3 women who rule my house - my wife and 2 young daughters. We love to read, cook, garden, travel, and explore together. POSTER PRESENTATION ASSIGNMENTS Please note your poster board assignment number. You should be available at your poster during the time indicated below. Abstracts on the following pages appear in the order of these poster board assignments. Time to be at your poster Poster Board # Assignment 1:00 – 1:45 1:45 – 2:30 1. Abrahamian. A X 2. Adams, J. X 3. Ale, J. X 4. Allen, J. X 5. Birch, J. X 6. Blaylock, J. X 7. Boskovic, O. X 8. Campbell, A. X 9. Carter, A. X 10. Clay, H. X 11. Daniels, A. X 12. Daniels, A. X 13. DelGiorno, A. X 14. Easterling, J. X 15. Findling, S. X 16. Finnegan, R. X 17. Franko, J. X Poster Board # Assignment 1:00 – 1:45 1:45 – 2:30 18. Gipson, D. X 19. Gajtka, T. X 20. Gorugantula, S. X 21. Gravely, S. X 22. Greene, J. X 23. Hall, J. X 24. Haynes, D. X 25. Hendrickson, A. X 26. Isme, M. X 27. Johnson, D. X 28. Johnson, M. X 29. Judy, C. X 30. Justus, R. X 31. Kafley, B. X 32. Katrib, A. X 33. King, A. X 34. Kirby, B. X 35. Li, B. X 36. Loughman, K. X 37. Lyda, L. X 38. Mahavadi, S. X 39. Malipur, B. X Poster Board # Assignment 1:00 – 1:45 1:45 – 2:30 40. Martin, R. X 41. Mazagri, H. X . 42. Mazzella, L. X 43. Mitchell, N. X 44. Phair, B. X 45. Price, R. X 46. Reddy, R. X 47. Richmond, S. X 48. Roberts, A. X 49. Rosas, E. X 50. Schmitt, D. X 51. Schnelle, A. X 52. Shrestha, S. X 53. Simmons, K. X 54. Sisson, K. X 55. Stankus, D. X 56. Vu, L. X 57. Wilson, A. X 58. Winkie, C. X POSTER ABSTRACT TITLES and AUTHORS 1. Which Type of Aggregate of Alpha Synuclein Increases the Permeability of the Rough Endoplasmic Reticulum to Small Molecules? Andy Abrahamian, William Wonderlin. Department of Biochemistry, Robert C. Byrd Health Science Center, West Virginia University, Morgantown, WV. 2. Development of a Bacteria Based Tumor Diagnostic Agent for Colon Cancer. Jeffrey P. Adams, Rachel L. Justus, Joan C. Olson PhD. The Robert C. Byrd Health Sciences Center of West Virginia University. 3. Investigating the potential use of Cerium Oxide nanoparticles for the treatment of sepsis polymicrobial sepsis. Jaya K. Ale, Dr.Eric Blough, Erin Fankhanel. 1Department of Biology 2Department of Pharmacology, Physiology and Toxicology, Joan C. Edwards School of Medicine, 3Center for Diagnostic Nanosystems; 4Department of Pharmaceutical Science and Research, School of Pharmacy Services, Marshall University, Huntington, WV, USA- 25755. 4. Investigating the Role of Vascular Endothelial Growth Factor in Adipocyte Specific VEGF Deficient Mice. Jessica Allen1,3, Nicole Zachwieja2, Janelle Stricker2, Linda Vona-Davis1, and Mark Olfert2. 1Departments of Surgery and 2Exercise Physiology, Mary Babb Randolph Cancer Center, West Virginia University, Morgantown and 3Concord University, Athens, WV. 5. The role of the erythrocyte cytoskeleton during invasion by Francisella tularensis. James Birch, Deanna Schmitt, and Joseph Horzempa; Department of Natural Sciences and Mathematics, West Liberty University. 6. Response of EMP2, ID2 and TM4SF1 Expression to Female Steroid Hormones and Epigenetic Agents in Meningioma Cells. Julie A. Blaylock†*, Stephen L. Heywood*, Bradley Schell*, Anojinie Karunathilake*, Gerald R. Hankins *. † South Charleston High School, 1 Eagle Way, South Charleston, WV 25309; * Department of Biology, West Virginia State University, Institute, WV 25112. 7. Evaluating the role of mitochondria in HIB brown adipose cells Olivia Boskovic*, Tamara Trout*, Hasan Koc+ and Emine C. Koc* *Department of Biochemistry and Microbiology, Marshall University School of Medicine, Huntington, WV; +Department of Pharmaceutical Research and Science, School of Pharmacy, Huntington, WV. 8. Adult Neural Stem Cell Differentiation after Phthalate Exposure. Amber N. Campbell, Nadja Spitzer, Ph.D.; Department of Biological Sciences, Marshall University, Huntington, WV. 9. Biological Engineering of a Neural Migratory Stream. Arrin B. Carter and Elmer M. Price. Department of Biology, College of Science, Marshall University, Huntington, WV. 10. Extraction and isolation of phytoalexins from Centaurea nigrescens exhibit inhibitory action against Staphylococcus aureus. Haven Clay, Notashia Baughman, Patrick C. Milkowski, Jared Rock, Katharine E. Lambson, Luke G. Huggins. West Virginia Wesleyan College, Buckhannon, WV 26201. 11. Inhibitory Effect of Baicalin and Baicalein on Ovarian cancer cells. Adrienne Daniels*, Tasha Howard*, Jianchu Chen, Gary O.
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