The impact of Pleistocene climate change on an ancient arctic–alpine plant: multiple lineages of disparate history in Oxyria digyna Geraldine A. Allen1, Kendrick L. Marr2, Laurie J. McCormick1 & Richard J. Hebda1,2 1Department of Biology, University of Victoria, Victoria, B.C., Canada V8W 3N5 2Royal British Columbia Museum, 675 Belleville St., Victoria, B.C. Canada V8W 9W2 Keywords Abstract Arctic-alpine plants, cpDNA, phylogeography, Pleistocene glaciations, refugia. The ranges of arctic–alpine species have shifted extensively with Pleistocene cli- mate changes and glaciations. Using sequence data from the trnH-psbA and trnT- Correspondence trnL chloroplast DNA spacer regions, we investigated the phylogeography of the Geraldine Allen, Department of Biology, widespread, ancient (>3 million years) arctic–alpine plant Oxyria digyna (Polyg- University of Victoria, Victoria, B.C., Canada onaceae). We identified 45 haplotypes and six highly divergent major lineages; V8W 3N5. Tel: 1-250-721-7110; Fax: estimated ages of these lineages (time to most recent common ancestor, T ) 1-250-721-7120; E-mail:
[email protected] MRCA ranged from ∼0.5 to 2.5 million years. One lineage is widespread in the arctic, a Funded by the John and Joan Walton secondisrestrictedtothesouthernRockyMountainsofthewesternUnitedStates, Innovation Fund, the October Hill Foundation, and a third was found only in the Himalayan and Altai regions of Asia. Three other the Royal British Columbia Museum and by lineages are widespread in western North America, where they overlap extensively. Discovery Grants from the Natural Sciences and Engineering Research Council of Canada. The high genetic diversity and the presence of divergent major cpDNA lineages within Oxyria digyna reflect its age and suggest that it was widespread during much Received: 7 October 2011; Revised: 20 of its history.