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ISSN: 0191-2917 e-ISSN: 1943-7692

SEARCH Editor-in-Chief: Alexander V. Karasev Enter Keywords Published by The American Phytopathological Society MPMI Home > Plant Disease > Table of Contents > Abstract Quick Links Phytobiomes Previous Article | Next Article Phytopathology Add to favorites

Plant Disease June 2012, Volume 96, Number 6 E-mail to a colleague Plant Health Page 917 Progress https://doi.org/10.1094/PDIS-02-12-0147-PDN Alert me when new articles cite this article

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Related articles First Report of Pepper mottle virus Infecting found in APS Journals Resources

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About Plant Disease M. J. Melzer, J. S. Sugano, D. Cabanas, K. K. Dey, B. Kandouh, D. Mauro, I. First Look Rushanaedy, S. Srivastava, S. Watanabe, and W. B. Borth, Plant and Environmental Most Downloaded Protection Sciences, University of Hawaii, Honolulu96822; S. Tripathi, T. Matsumoto, L. Articles Keith, and D. Gonsalves, USDA-ARS Pacific Basin Agricultural Research Center, Hilo, HI 96720; and J. S. Hu, Plant and Environmental Protection Sciences, University of Hawaii, Journals Impact Honolulu, HI 96822 Submit a Manuscript

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About My Password Open Access. Rights and Permissions In August 2011, tomato (Solanum lycopersicum L.) fruit from a University of Hawaii field trial Plagiarism and Ethics displayed mottling symptoms similar to that caused by Tomato spotted wilt virus (TSWV) or Advertise other tospoviruses. The foliage from affected plants, however, appeared symptomless. Fruit and leaf tissue from affected plants were negative for TSWV analyzed by double antibody Open Access sandwich (DAS)-ELISA and/or TSWV ImmunoStrips (Agdia, Elkhart, IN) when performed following the manufacturer's instructions. Total RNA from a symptomatic and an ORCID Registry asymptomatic plant was isolated using an RNeasy Plant Mini Kit (Qiagen, Valencia, CA) and reverse transcribed using Invitrogen SuperScript III reverse transcriptase (Life Technologies,

Grand Island, NY) and primer 900 (5′- CACTCCCTATTATCCAGG(T)16-3′) following the enzyme manufacturer's instructions. The cDNA was then used as template in a universal potyvirus PCR assay using primers 900 and Sprimer, which amplify sequences encoding the partial inclusion body protein (NIb), coat protein, and 3′ untranslated region of potyviruses (1). A ~1,700-bp product was amplified from the cDNA of the symptomatic plant but not the asymptomatic plant. This product was cloned using pGEM-T Easy (Promega, Madison, WI) and three clones were sequenced at the University of Hawaii's Advanced Studies in Genomics, Proteomics, and Bioinformatics laboratory. The 1,747-bp consensus sequence of the three clones was deposited in GenBank (Accession No. JQ429788) and, following primer sequence trimming, found to be 97% identical to positions 7,934 through 9,640 of Pepper mottle virus (PepMoV; family Potyviridae, genus Potyvirus) accessions from Korea (isolate ‘217’ from tomato; EU586126) and California (isolate ‘C’ from pepper; M96425). To determine the incidence of PepMoV in the field trial, all 292 plants representing 14 tomato cultivars were assayed for the virus 17 weeks after planting using a PepMoV-specific DAS- ELISA (Agdia) following the manufacturer's directions. Plants were considered positive if their mean absorbance at 405 nm was greater than the mean absorbance + 3 standard deviations + 10% of the negative control samples. The virus incidence ranged from 4.8 to 47.6% for the different varieties, with an overall incidence of 19.9%. Although plant growth was not noticeably impaired by PepMoV infection, the majority of fruit from infected plants was unsaleable, making PepMoV a considerable threat to tomato production in Hawaii. PepMoV has been reported to naturally infect tomato in Guatemala (3) and South Korea (2). To our knowledge, this is the first report of this virus in Hawaii and the first report of this virus naturally infecting tomato in the United States.

References: (1) J. Chen et al. Arch. Virol. 146:757, 2001. (2) M.-K. Kim et al. Plant Pathol. J. 24:152, 2008. (3) J. Th. J. Verhoeven et al. Plant Dis. 86:186, 2002.

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First Report of Apple of Peru (Nicandra physalodes) Infected with Pepper Mottle Virus in Hawaii D. Wang, I. Hamim, W. B. Borth, M. J. Melzer, J. Y. Suzuki, M. M. Wall, T. Matsumoto, G. F. Sun, and J. S. Hu Plant Disease Jan 2019, Volume 103, Number 1, 169-169 Citation | Full Text HTML |

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