insects Article A Transcriptome Survey Spanning Life Stages and Sexes of the Harlequin Bug, Murgantia histrionica Michael E. Sparks 1, Joshua H. Rhoades 1, David R. Nelson 2, Daniel Kuhar 1, Jason Lancaster 3, Bryan Lehner 3, Dorothea Tholl 3, Donald C. Weber 1 and Dawn E. Gundersen-Rindal 1,* 1 Invasive Insect Biocontrol and Behavior Laboratory, USDA-ARS, Beltsville, MD 20705, USA;
[email protected] (M.E.S.);
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[email protected] (D.C.W.) 2 Department of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science Center, Memphis, TN 38163, USA;
[email protected] 3 Department of Biological Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA;
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[email protected] (D.T.) * Correspondence:
[email protected]; Tel.: +1-301-504-6692 Academic Editor: Brian T. Forschler Received: 27 March 2017; Accepted: 18 May 2017; Published: 25 May 2017 Abstract: The harlequin bug, Murgantia histrionica (Hahn), is an agricultural pest in the continental United States, particularly in southern states. Reliable gene sequence data are especially useful to the development of species-specific, environmentally friendly molecular biopesticides and effective biolures for this insect. Here, mRNAs were sampled from whole insects at the 2nd and 4th nymphal instars, as well as sexed adults, and sequenced using Illumina RNA-Seq technology. A global assembly of these data identified 72,540 putative unique transcripts bearing high levels of similarity to transcripts identified in other taxa, with over 99% of conserved single-copy orthologs among insects being detected.