In Vitro Callus Culture of Dianthus Chinensis L. for Assessment of Flavonoid Related Gene Expression Prole R. Sreelekshmi University of Kerala Elenjikkal A Siril (
[email protected] ) University of Kerala https://orcid.org/0000-0002-4956-8428 Research Article Keywords: China pink, In vitro avonoid production, Friable callus, 2,4- D, Chalcone synthase Posted Date: March 17th, 2021 DOI: https://doi.org/10.21203/rs.3.rs-320486/v1 License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License Page 1/27 Abstract Dianthus chinensis L. is an edible, ornamental herb used to prepare the Dianthi Herba, a Chinese traditional rejuvenating medicine. Owing to the rapid proliferation of callus tissues, in vitro production of avonoids has their own specic importance. Callus cultures raised followed by auxin directed biosynthesis of avonoid through related transcript prole were carried out. Murashige and Skoog (MS) medium fortied with 2,4- Dichlorophenoxy acetic acid (2,4- D) or picloram induced formation of friable callus from internode derived cultures of D. chinensis. Culture medium containing 2,4- D (10 µM) produced the highest avonoid content, 4.44 mg quercetin equivalent per gram (QE g− 1) under incubation in continuous dark condition, while maximum dry weight yield (0.38 g/ culture) was obtained from 10 µM 2,4- D under 16 h light / 8 h dark condition (50 µmol m− 2 s− 1 irradiance) at 60 days of incubation. The callus raised in light condition in 10 µM 2,4- D selected to analyze avonoid related gene expression prole viz., chalcone synthase (CHS), chalcone isomerase (CHI), avanone-3-hydroxylase (F3H), and avonol synthase (FLS) at specic time intervals.