DOCSLIB.ORG

Sequences Support Recognition of Papilio Chikae Igarashi and Papilio Hermeli Nuyda As Distinct Species

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Sequences Support Recognition of Papilio Chikae Igarashi and Papilio Hermeli Nuyda As Distinct Species Philippine Journal of Science 150 (S1): 111-119, Special Issue on Biodiversity ISSN 0031 - 7683 Date Received: 07 Sep 2020 Cytochrome C Oxidase Subunit I and II (COI and COII) Sequences Support Recognition of Papilio chikae Igarashi and Papilio hermeli Nuyda as Distinct Species John Vincent B. Cabusas1, Aimee-Lynn A. Barrion-Dupo2,3, Romnick A. Latina1, and Barbara L. Caoili1* 1Institute of Weed Science, Entomology, and Plant Pathology College of Agriculture and Food Science 2Environmental Biology Division, Institute of Biological Sciences College of Arts and Sciences 3Entomology Section, Museum of Natural History University of the Philippines Los Baños College, Laguna 4031 Philippines Papilio chikae and P. hermeli are two endemic swallowtail butterflies in the Philippines found primarily in the Cordillera region and Mindoro Island, respectively. Their species status is still subject to debate due to opposing interpretations of their genitalia and morphological traits. This study aims to delineate these taxa through the use of mitochondrial cytochrome C oxidase subunit I (COI) gene marker, with additional information on their cytochrome C oxidase subunit II (COII) gene. The COI nucleotide sequences from our Philippine P. chikae samples were surprisingly identical to P. hermeli EF514465.1 on the GenBank, whereas our P. hermeli samples showed a 100% similarity with P. hermeli EF514464.1. Based on our phylogenetic analysis, P. chikae and P. hermeli exhibit distinct COI barcodes supported by a high bootstrap value (85%). The P. hermeli voucher specimen with GenBank Acc. No. EF514465.1 clustered with our P. chikae samples, separate from the cluster containing our P. hermeli specimens. Nonetheless, the 3.1–3.4% genetic distance observed in the COI region supported by the formation of two separate clusters suggests species status for these taxa. Moreover, species-specific molecular markers obtained from the COII region displayed a 2.4% distance between these two taxa. These molecular markers could therefore be utilized to distinguish P. chikae from P. hermeli. Nevertheless, a thorough examination of their morphology, behavior, and ecology is still imperative to firmly establish their taxonomic status. Keywords: divergence rate, DNA barcodes, Luzon peacock swallowtail butterfly, species delineation INTRODUCTION account of their high species richness and endemism, well-documented taxonomy, and a high degree of host- Unraveling diversification patterns and biogeographical plant specialization (Condamine et al. 2012). Currently, history has been partly made possible by studying ideal subgenus Achillides is comprised of up to 25 species and candidates, such as species of Papilio (Achillides) at least a hundred subspecies distributed across Asia, butterflies, also known as “peacock swallowtails,” on particularly the Indo-Australian archipelago. *Corresponding Author: [email protected] 111 Philippine Journal of Science Cabusas et al.: DNA Barcodes of Distinct Luzon Vol. 150 No. S1, Special Issue on Biodiversity Peacock Swallowtails In the Philippines, endemism has been documented on and Treadaway (2004) as a standard nomenclature two Papilio species, commonly referred to as Luzon reference of Philippine Papilionidae, CITES (Convention peacock swallowtails. P. chikae Igarashi was first on International Trade in Endangered Species of Wild discovered in 1965 in Mount Santo Tomas, and later near Fauna and Flora) also proposed to amend the current the peaks of Benguet, Mountain Province, and Southern listing of P. chikae to A. chikae chikae (CITES 2018). The Cordillera (Page and Treadaway 2004). P. hermeli – absence of papiliochrome wing pigments in Achillides which was first described by Nuyda in 1992 – has been (present in other subgenera of Papilio) was used as a observed to inhabit Mount Halcon and Mount Baco, basis for the amendments, in addition to its distinct larval situated on the northern and southern parts of Mindoro and pupal forms (Igarashi and Fukuda 2000; Page and Island, respectively (Nuyda 1992; Treadaway 1995; Treadaway 2004). Bauer and Frankenbach 1998; Page and Treadaway 2004; Treadaway and Schröder 2012). Both peacock Delineation of species using mitochondrial genes has swallowtails are present at montane forests at high been preferred due to fewer intron interferences, few altitudes where ferns, lichens, orchids, and mosses thrive recombinations, and its haploid transmission (Hebert (Page and Treadaway 2003). They are speculated to feed et al. 2003). The COI gene is nowadays commonly on Euodia spp., which belong to the Family Rutaceae used to identify lepidopteran species known for low (Igarashi 1984; Page and Treadaway 2004). sequence divergence (Hebert et al. 2003; Hajibabaei et al. 2006; Litman et al. 2018). Combined molecular and Papilio chikae and P. hermeli share many similar traits. morphological analyses of lepidopterans have been shown Golden-green scales are evenly scattered throughout to increase the rate of species identification than using their black body and wings on the dorsal side. Ventrally, either approach singularly (Litman et al. 2018). the dark-brown forewing has pronounced white bands that taper towards the inner margins, with dark veins The two Luzon peacock swallowtails have become spreading across the wing. The hindwing holds a series of alarmingly vulnerable to various threats from collectors, red, wavy lunules on the edges, mixed with bluish-green traders, researchers, and environmentally destructive patches along the sub-margin. The tail elongates towards practices by locals (Tsukada and Nishiyama 1982; the end, narrowing at its base. The underside of their Treadaway 1995; CITES 2018). P. chikae has been hindwing is black, where a portion is spotted by whitish included in the International Union for Conservation of scales marked by pale purple spots enclosed on red-orange Nature (IUCN) Red Data Book of threatened species, lunules along the margins (Collins and Morris 1985). The CITES list of endangered species, and Appendix A of female has more red spots on the dorsal hindwing than European Union regulation on trading with species the male, but the male has larger bluish-green patches. of wild fauna and flora. Recent proposals have also The two swallowtails, however, are differentiated based been made to include P. hermeli in the CITES list of on the distinctive whitish-gray bands on the ventral side endangered species (CITES 2018). Hence, this study of the forewing of P. hermeli, which in P. chikae is less was designed to provide baseline information relevant apparent, and tapers to pale blue in the inner margin of to the conservation efforts and strategies of these the forewing (CITES 2018). swallowtails. In the context of these two Luzon peacock swallowtails, their molecular data could be used in Disagreement over the taxonomic ranking of these inferring phylogenies through the use of analytical two taxa has persisted for decades. Some authors methods such as the maximum likelihood (ML), which treated the differences in genitalia across sexes of provides an array of sophisticated evolutionary models these two swallowtails as significant, thus classifying fit for understanding sequence evolution of organisms them as separate species (Treadaway 1995; Bauer (Yang and Rannala 2012). While mitochondrial COII and Frankenbach 1998; Häuser et al. 2005). Others sequences were unavailable for both P. chikae and asserted the conspecificity of these two taxa, noting a P. hermeli prior to this study, COI sequences were close resemblance in their appearance, granting them available on GenBank for P. hermeli, but none for separation at subspecies level (Page and Treadaway P. chikae (Fontanilla et al. 2014). The objectives of 2004; CITES 2018). The subspecies concept was this study were: 1) to compare the Luzon peacock created to characterize geographical variations based swallowtails specimens based on partial mitochondrial on morphology but objective metrics to discriminate COI and COII nucleotide sequences; 2) to compare subspecies are lacking, failing to show unique derived nucleotide and amino acid sequences; and 3) to evolutionary units (Braby et al. 2012). In 2004, Page construct a phylogenetic tree based on these molecular and Treadaway elevated Achillides from subgenus to operational taxonomic units. genus level, changing the nomenclature of Papilio hermeli to Achillides chikae hermeli. Referring to Page 112 Philippine Journal of Science Cabusas et al.: DNA Barcodes of Distinct Luzon Vol. 150 No. S1, Special Issue on Biodiversity Peacock Swallowtails MATERIALS AND METHODS model and illustrated via ML using MEGA7 software version 7.0.26 with 1,000 bootstrap replications (Kumar et al. 2016). Source of Test Insects Voucher specimens of two individuals each of Papilio chikae (UPLBMNH LEP-04639 and UPLBMNH LEP- 04640) and P. hermeli (UPLBMNH LEP-04641 and LEP-04642) were provided by the Museum of Natural RESULTS History, University of the Philippines Los Baños (UPLB), The partial COI and COII gene fragments with molecular College, Laguna. sizes of approximately 700–750 base pairs (bp) and 800 bp, respectively were amplified. Upon trimming, Molecular Identification of the Swallowtail Species a 658-nucleotide long partial COI gene sequence and Molecular analyses were conducted at the Insect 676-nucleotide-long partial COII gene were generated Pathology and Molecular Biology Laboratory, Institute for all the specimens. of Weed Science, Entomology, and Plant Pathology, College of Agriculture and Food
Load more

Recommended publications
  • Inertia in CITES Nomenclature Nomenclature
    Letter Inertia in CITES nomenclature nomenclature. African forest elephant (Loxodonta cy- clotis) has been splitted from African savannah elephant To prevent international commecial trade from (L. africana) for about 20 years (Roca et al. 2001). negatively affecting the survival of wild species, the Con- Early in 2002, the Nomenclature Committee of CITES vention on International Trade in Endangered Species of recognized that the subspecies L. africana cyclotis Wild Fauna and Flora (CITES) lists roughly 35,800 species may be a full species (CITES 2002). However, forest in its appendices, which are updated at each Conference elephant is still merged as a subspecies of L. africana of the Parties (CoP). Frank and Wilcove (2019) used under CITES. Due to its slow growth rate and low pangolins (Manis spp.) to illustrate how delay in population numbers, African forest elephants are more updating CITES appendices may hinder the conservation sensitive to human-induced mortality than savanna of traded species. All 8 pangolin species have been elephants, and their populations have declined rapidly uplisted to Appendix I, and new pangolin species, once in the past decade (Poulsen et al. 2017; Turkalo et al. discovered, are listed in Appendix II. Species listings 2017). The convention and its projects, such as the in CITES appendices are determined by both threat Monitoring the Illegal Killing of Elephants (MIKE), need status and the nomenclatural system CITES uses. The to treat the forest elephant as a full species, reassess its convention adopts nomenclatural standard references populations, and reanalyze the data (Groves 2016), so for different taxa at each CoP meeting.
    [Show full text]
  • Checklist of Fish and Invertebrates Listed in the CITES Appendices
    JOINTS NATURE \=^ CONSERVATION COMMITTEE Checklist of fish and mvertebrates Usted in the CITES appendices JNCC REPORT (SSN0963-«OStl JOINT NATURE CONSERVATION COMMITTEE Report distribution Report Number: No. 238 Contract Number/JNCC project number: F7 1-12-332 Date received: 9 June 1995 Report tide: Checklist of fish and invertebrates listed in the CITES appendices Contract tide: Revised Checklists of CITES species database Contractor: World Conservation Monitoring Centre 219 Huntingdon Road, Cambridge, CB3 ODL Comments: A further fish and invertebrate edition in the Checklist series begun by NCC in 1979, revised and brought up to date with current CITES listings Restrictions: Distribution: JNCC report collection 2 copies Nature Conservancy Council for England, HQ, Library 1 copy Scottish Natural Heritage, HQ, Library 1 copy Countryside Council for Wales, HQ, Library 1 copy A T Smail, Copyright Libraries Agent, 100 Euston Road, London, NWl 2HQ 5 copies British Library, Legal Deposit Office, Boston Spa, Wetherby, West Yorkshire, LS23 7BQ 1 copy Chadwick-Healey Ltd, Cambridge Place, Cambridge, CB2 INR 1 copy BIOSIS UK, Garforth House, 54 Michlegate, York, YOl ILF 1 copy CITES Management and Scientific Authorities of EC Member States total 30 copies CITES Authorities, UK Dependencies total 13 copies CITES Secretariat 5 copies CITES Animals Committee chairman 1 copy European Commission DG Xl/D/2 1 copy World Conservation Monitoring Centre 20 copies TRAFFIC International 5 copies Animal Quarantine Station, Heathrow 1 copy Department of the Environment (GWD) 5 copies Foreign & Commonwealth Office (ESED) 1 copy HM Customs & Excise 3 copies M Bradley Taylor (ACPO) 1 copy ^\(\\ Joint Nature Conservation Committee Report No.
    [Show full text]
  • Read the Comments
    CropLife * AMERICA * ~ March 2,2015 VIA FEDERAL E-RULEMAKING PORTAL Public Comments Processing Attn: FWS-R3-ES-2014-0056 U.S. Fish and Wildlife Service Headquarters MS: BPHC 5275 Leesburg Pike Falls Church, VA 22041-3803 Re: Initial Comments: 90-Day Finding on a Petition to List the Monarch Butterfly (Danaus Plexippus Plexippus) as Threatened Under the Endangered Species Act, 79 Fed. Reg. 78775 (Dec. 31, 2014) Dear Sir or Madam, CropLife America ("CropLife") is the national voice of the agricultural crop protection industry. CropLife represents companies that develop, manufacture, and distribute virtually all ofthe crop protection, pest management, and biotechnology products used by American farmers. Because these products are critical technologies for American agriculture, CropLife's members have a substantial interest in the issues presented in the 90-day finding on a petition to list as threatened the Monarch butterfly (Dana us plexippus p/exippus) made available to the publicI by the U.S. Fish and Wildlife Service ("FWS") on December 31, 2014 pursuant to the Endangered Species Act ("ESA,,). 2 As these initial comments indicate, our organization and its members believe that the proposed listing of the Monarch butterfly under the ESA is not warranted. Estimates for Monarch populations in North America have been available for only about two decades. CropLife and its members understand that overall estimated population levels in North America have declined during that period, although the data indicate that Monarch population numbers fluctuate very widely from year-to-year.3 Indeed, just this past year, the Eastern North I See Endangered and Threatened Wildlife and Plants; 90-Day Findings on Two Petitions, 79 Fed.
    [Show full text]
  • Namadgi National Park Plan of Management 2010
    PLAN OF MANAGEMENT 2010 Namadgi National Park Namadgi National NAMADGI NATIONAL PARK PLAN OF MANAGEMENT 2010 NAMADGI NATIONAL PARK PLAN OF MANAGEMENT 2010 NAMADGI NATIONAL PARK PLAN OF MANAGEMENT 2010 © Australian Capital Territory, Canberra 2010 ISBN 978-0-642-60526-9 Conservation Series: ISSN 1036-0441: 22 This work is copyright. Apart from any use as permitted under the Copyright Act 1968, no part may be reproduced without the written permission of Land Management and Planning Division, Department of Territory and Municipal Services, GPO Box 158, Canberra ACT 2601. Disclaimer: Any representation, statement, opinion, advice, information or data expressed or implied in this publication is made in good faith but on the basis that the ACT Government, its agents and employees are not liable (whether by reason or negligence, lack of care or otherwise) to any person for any damage or loss whatsoever which has occurred or may occur in relation to that person taking or not taking (as the case may be) action in respect of any representation, statement, advice, information or date referred to above. Published by Land Management and Planning Division (10/0386) Department of Territory and Municipal Services Enquiries: Phone Canberra Connect on 13 22 81 Website: www.tams.act.gov.au Design: Big Island Graphics, Canberra Printed on recycled paper CONTENTS NAMADGI NATIONAL PARK PLAN OF MANAGEMENT 2010 Contents Acknowledgments ...............................................................................................................................
    [Show full text]
  • Back Mr. Rudkin: Differentiating Papilio Zelicaon and Papilio Polyxenes in Southern California (Lepidoptera: Papilionidae)
    Zootaxa 4877 (3): 422–428 ISSN 1175-5326 (print edition) https://www.mapress.com/j/zt/ Article ZOOTAXA Copyright © 2020 Magnolia Press ISSN 1175-5334 (online edition) https://doi.org/10.11646/zootaxa.4877.3.3 http://zoobank.org/urn:lsid:zoobank.org:pub:E7D8B2D6-8E1B-4222-8589-EAACB4A65944 Welcome back Mr. Rudkin: differentiating Papilio zelicaon and Papilio polyxenes in Southern California (Lepidoptera: Papilionidae) KOJIRO SHIRAIWA1 & NICK V. GRISHIN2 113634 SW King Lear Way, King City, OR 97224, USA. https://orcid.org/0000-0002-6235-634X 2Howard Hughes Medical Institute and Departments of Biophysics and Biochemistry, University of Texas Southwestern Medical Cen- ter, 5323 Harry Hines Blvd, Dallas, TX 75390-9050, USA. https://orcid.org/0000-0003-4108-1153 Abstract We studied wing pattern characters to distinguish closely related sympatric species Papilio zelicaon Lucas, 1852 and Papilio polyxenes Fabricius, 1775 in Southern California, and developed a morphometric method based on the ventral black postmedian band. Application of this method to the holotype of Papilio [Zolicaon variety] Coloro W. G. Wright, 1905, the name currently applied to the P. polyxenes populations, revealed that it is a P. zelicaon specimen. The name for western US polyxenes subspecies thus becomes Papilio polyxenes rudkini (F. & R. Chermock, 1981), reinstated status, and we place coloro as a junior subjective synonym of P. zelicaon. Furthermore, we sequenced mitochondrial DNA COI barcodes of rudkini and coloro holotypes and compared them with those of polyxenes and zelicaon specimens, confirming rudkini as polyxenes and coloro as zelicaon. Key words: Taxonomy, field marks, swallowtail butterflies, desert, sister species Introduction Charles Nathan Rudkin, born 1892 at Meriden, Connecticut was a passionate scholar of history of the West, espe- cially the Southwestern region.
    [Show full text]
  • Arthropods of Elm Fork Preserve
    Arthropods of Elm Fork Preserve Arthropods are characterized by having jointed limbs and exoskeletons. They include a diverse assortment of creatures: Insects, spiders, crustaceans (crayfish, crabs, pill bugs), centipedes and millipedes among others. Column Headings Scientific Name: The phenomenal diversity of arthropods, creates numerous difficulties in the determination of species. Positive identification is often achieved only by specialists using obscure monographs to ‘key out’ a species by examining microscopic differences in anatomy. For our purposes in this survey of the fauna, classification at a lower level of resolution still yields valuable information. For instance, knowing that ant lions belong to the Family, Myrmeleontidae, allows us to quickly look them up on the Internet and be confident we are not being fooled by a common name that may also apply to some other, unrelated something. With the Family name firmly in hand, we may explore the natural history of ant lions without needing to know exactly which species we are viewing. In some instances identification is only readily available at an even higher ranking such as Class. Millipedes are in the Class Diplopoda. There are many Orders (O) of millipedes and they are not easily differentiated so this entry is best left at the rank of Class. A great deal of taxonomic reorganization has been occurring lately with advances in DNA analysis pointing out underlying connections and differences that were previously unrealized. For this reason, all other rankings aside from Family, Genus and Species have been omitted from the interior of the tables since many of these ranks are in a state of flux.
    [Show full text]
  • Samia Cynthia in New Jersey Book Review, Market- Place, Metamorphosis, Announcements, Membership Updates
    ________________________________________________________________________________________ Volume 61, Number 4 Winter 2019 www.lepsoc.org ________________________________________________________________________________________ Inside: Butterflies of Papua Southern Pearly Eyes in exotic Louisiana venue Philippine butterflies and moths: a new website The Lepidopterists’ Society collecting statement updated Lep Soc, Southern Lep Soc, and Assoc of Trop Lep combined meeting Butterfly vicariance in southeast Asia Samia cynthia in New Jersey Book Review, Market- place, Metamorphosis, Announcements, Membership Updates ... and more! ________________________________________________________________________________________ _________________________________________________________ Contents www.lepsoc.org ________________________________________________________ Digital Collecting -- Butterflies of Papua, Indonesia ____________________________________ Bill Berthet. .......................................................................................... 159 Volume 61, Number 4 Butterfly vicariance in Southeast Asia Winter 2019 John Grehan. ........................................................................................ 168 Metamorphosis. ....................................................................................... 171 The Lepidopterists’ Society is a non-profit ed- Membership Updates. ucational and scientific organization. The ob- Chris Grinter. ....................................................................................... 171
    [Show full text]
  • Assessment of Species Listing Proposals for CITES Cop18
    VKM Report 2019: 11 Assessment of species listing proposals for CITES CoP18 Scientific opinion of the Norwegian Scientific Committee for Food and Environment Utkast_dato Scientific opinion of the Norwegian Scientific Committee for Food and Environment (VKM) 15.03.2019 ISBN: 978-82-8259-327-4 ISSN: 2535-4019 Norwegian Scientific Committee for Food and Environment (VKM) Po 4404 Nydalen N – 0403 Oslo Norway Phone: +47 21 62 28 00 Email: [email protected] vkm.no vkm.no/english Cover photo: Public domain Suggested citation: VKM, Eli. K Rueness, Maria G. Asmyhr, Hugo de Boer, Katrine Eldegard, Anders Endrestøl, Claudia Junge, Paolo Momigliano, Inger E. Måren, Martin Whiting (2019) Assessment of Species listing proposals for CITES CoP18. Opinion of the Norwegian Scientific Committee for Food and Environment, ISBN:978-82-8259-327-4, Norwegian Scientific Committee for Food and Environment (VKM), Oslo, Norway. VKM Report 2019: 11 Utkast_dato Assessment of species listing proposals for CITES CoP18 Note that this report was finalised and submitted to the Norwegian Environment Agency on March 15, 2019. Any new data or information published after this date has not been included in the species assessments. Authors of the opinion VKM has appointed a project group consisting of four members of the VKM Panel on Alien Organisms and Trade in Endangered Species (CITES), five external experts, and one project leader from the VKM secretariat to answer the request from the Norwegian Environment Agengy. Members of the project group that contributed to the drafting of the opinion (in alphabetical order after chair of the project group): Eli K.
    [Show full text]
  • ACT, Australian Capital Territory
    Biodiversity Summary for NRM Regions Species List What is the summary for and where does it come from? This list has been produced by the Department of Sustainability, Environment, Water, Population and Communities (SEWPC) for the Natural Resource Management Spatial Information System. The list was produced using the AustralianAustralian Natural Natural Heritage Heritage Assessment Assessment Tool Tool (ANHAT), which analyses data from a range of plant and animal surveys and collections from across Australia to automatically generate a report for each NRM region. Data sources (Appendix 2) include national and state herbaria, museums, state governments, CSIRO, Birds Australia and a range of surveys conducted by or for DEWHA. For each family of plant and animal covered by ANHAT (Appendix 1), this document gives the number of species in the country and how many of them are found in the region. It also identifies species listed as Vulnerable, Critically Endangered, Endangered or Conservation Dependent under the EPBC Act. A biodiversity summary for this region is also available. For more information please see: www.environment.gov.au/heritage/anhat/index.html Limitations • ANHAT currently contains information on the distribution of over 30,000 Australian taxa. This includes all mammals, birds, reptiles, frogs and fish, 137 families of vascular plants (over 15,000 species) and a range of invertebrate groups. Groups notnot yet yet covered covered in inANHAT ANHAT are notnot included included in in the the list. list. • The data used come from authoritative sources, but they are not perfect. All species names have been confirmed as valid species names, but it is not possible to confirm all species locations.
    [Show full text]
  • Anicdotes • ISSUE 17 October 2020
    1 ISSUE 17 • October 2020 The official newsletter of the Australian National Insect Collection CSIRO NATIONAL FACILITIES AND COLLECTIONS www.csiro.au INSIDE THIS ISSUE The pandemic response issue David Yeates, Director The pandemic response issue ....................................... 1 We compile this issue as the dumpster fire of a year from Award from our CSIRO Business Unit, hell lurches through its final few months. Usually a vibrant Digital National Facilities and Collections. Welcome to new staff ...................................................2 community for entomologists from all over Australia and the These awards are always heavily world, ANIC has been an eerily quiet place during the depths ANIC wins DNFC 2020 award ........................................3 contested, not least because we are of the pandemic. All our Volunteers, Honorary Fellows, always competing against an army of very Visiting Scientists and Postgraduate Students were asked to Marvel flies a media hit .................................................3 compelling entries from the astronomers stay home. Visitors were not permitted. Under CSIRO’s COVID in DNFC. Congratulations to Andreas response planning, many of our staff worked from home. All our Australian Weevils Volume IV published ...................... 4 and the team. The second significant international trips were postponed, including the International achievement is the publication of Congress of Entomology in Helsinki in July. This has caused some Australian Weevils Volume 4, focussing on Donations: Phillip Sawyer Collection ............................5 David Yeates delay to research progress, as primary types held in overseas the broad-nosed weevils of the subfamily The Waite Institute nematodes come to ANIC ............ 6 institutions could not be examined and species identities could Entiminae. This is a very significant evolutionary radiation of not be confirmed.
    [Show full text]
  • Redalyc.On a New Species of the Genus Princeps Hübner, [1807
    SHILAP Revista de Lepidopterología ISSN: 0300-5267 [email protected] Sociedad Hispano-Luso-Americana de Lepidopterología España Bivar de Sousa, A.; Mendes, L.F. On a new species of the genus Princeps Hübner, [1807] from Cabinda (Angola) (Lepidoptera: Papilionidae) SHILAP Revista de Lepidopterología, vol. 37, núm. 147, septiembre, 2009, pp. 327-334 Sociedad Hispano-Luso-Americana de Lepidopterología Madrid, España Available in: http://www.redalyc.org/articulo.oa?id=45515238010 How to cite Complete issue Scientific Information System More information about this article Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Journal's homepage in redalyc.org Non-profit academic project, developed under the open access initiative 327-332 On a new species of the 7/9/09 15:12 Página 327 SHILAP Revta. lepid., 37 (147), septiembre 2009: 327-334 CODEN: SRLPEF ISSN:0300-5267 On a new species of the genus Princeps Hübner, [1807] from Cabinda (Angola) (Lepidoptera: Papilionidae) A. Bivar de Sousa & L.F. Mendes Abstract A new species of the genus Princeps Hübner, [1807] of the “zenobia group” is described upon two males collected in the primary forest of Cabinda (Angola) and originally assigned to “Papilio cypraeofila”; it is compared with the remaining species of the group though it seems particularly close to Princeps (Druryia) cyproeofila (Butler, 1868) and to P. (D.) filaprae (Suffert, 1904). KEY WORDS: Lepidoptera, Papilionidae, Princeps, Druryia, zenobia-group, new species, Angola. Sobre una nueva especie del género Princeps Hübner, [1807] de Cabinda (Angola) (Lepidoptera: Papilionidae) Resumen Se describe una nueva especie del género Princeps Hübner, [1807] del “grupo zenobia” sobre dos machos cogidos en la floresta primaria de Cabinda (Angola) y originalmente determinados como “Papilio cypraeofila”; se la compara con todas las especies del grupo, aunque probablemente sea más próxima a Princeps (Druryia) cyproeofila (Butler, 1868) y a P.
    [Show full text]
  • The Landcare Perspective the Quarterly Newsletter of the Upper Shoalhaven and Upper Deua Catchments
    Biodiversity Special Issue SUMMER 2016 The Landcare Perspective The Quarterly Newsletter of the Upper Shoalhaven and Upper Deua Catchments are dull coloured, with browns and DATE Butterflies of the Upper yellows predominating. They have a Summer 2016 rapid wingbeat and a Shoalhaven quick, moth-like flight, Inside this issue: Kim Pullen often near ground level. Caterpillars of Butterflies of the Upper 1, The insect fauna of the Upper Shoalhaven is the two species of Shoalhaven 3,11 that which is characteristic of the cold, Trapezites phigalioides open grassy valleys and wooded ridges of ‘Flats’ feed on a range Updates 2 Source: OEH: Shane the Southern Tablelands of NSW, together of broad-leaved Ruming Green Army 3 with some coastal elements penetrating plants, but those of Australian Native Bees 4, 5 along the eastern escarpment. This short the remainder, 7, 9 treatment will cover the southern part of comprising Ochres Braidwood Bird Surveys the catchment centered on Braidwood, (Trapezites), Grass- 2015 - Canberra 6 home to about 70 butterfly species. It will skippers (Toxidia), Ornithological Group describe some of the more commonly seen Shield-skippers Emerging Weeds: 8 species, as well as (Signeta), Sedge- Ox eye daisy some other notable skippers (Hesperilla, Imperial Jezebel Delias harpalyce Photographer: Jembaicumbene 1859 - 9 Oreisplanus) and but less common Russel Best William Stanley Jevons ones. Grass-darts (Ocybadistes, Suniana, Taractrocera), Wombats - Vombatus 10, In the Upper ursinus 11 among others, have monocotyledonous Shoalhaven there are host plants, mainly grasses and sedges. Contact Biodiversity five families of Manager Felicity Sturgiss butterflies: Skippers Most of the 17 Australian Swallowtail Ph: 0427 111 101 (Hesperiidae) with species are tropical, and only the [email protected] about 27 species; Macleay’s (see image left) and Orchard Biodiversity Website: Swallowtails Swallowtails reach our region.
    [Show full text]