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CSH5 YES, QUALITY IS YOUR FIRST CHOICE FOR RNase HI!
1 2 3 4 5 6 Purity: Single Band on PAGE
phosphorylase B Easy to Use: 1 - 2 units / !~1 bovine serum albumin
ovalbumin Convenient: 3 Package Size available carbonic anhydrase 50, 100 and 400 units soybean trypsin inhibitor
lysozyme
Lane 1. The protein standards for molecular weight markers 2. The total soluble proteins from E. coli clone 3. The proteins purified by the first ion exchange chromatography 4. The more purified proteins by the second ion exchange chroma, tography 5. The purified RNase H by the final purification of gel filtration No other protein appeared on PAGE 6. Same as Lane I
Ribonuclease H from E. coli is an endonuclease that specifically degrades the RNA strand of DNA-RNA hybrids. RNase H is a key enzyme in several procedures including:
- Amplification by self-sustained sequence replication (3SR) - cDNA synthesis and <:loning where it is used to remove mRNA during second strand cDNA synthesis - Removal of poly (A) tails of mRNAs after hybridization with poly dT
"FOR RESEARCH USE ONLY. NOT FOR DRUG OR OTHER USES"
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