Original Paper Czech Journal of Genetics and Plant Breeding, 57, 2021 (2): 58–66 https://doi.org/10.17221/88/2020-CJGPB Phylogenetics of native conifer species in Vietnam based on two chloroplast gene regions rbcL and matK Mai Phuong Pham1, Viet Ha Tran2, Dinh Duy Vu1,3*, Quoc Khanh Nguyen1, Syed Noor Muhammad Shah4* 1Vietnam-Russia Tropical Centre, Hanoi, Vietnam 2College of Forestry, Vietnam National University of Forestry, Hanoi, Vietnam 3Graduate University of Science and Technology (GUST), Vietnam Academy of Science and Technology (VAST), Hanoi, Vietnam 4Department of Horticulture, Faculty of Agriculture, Gomal University, Dera Ismail Khan, Pakistan *Corresponding authors:
[email protected];
[email protected] Mai Phuong Pham and Viet Ha Tran contributed equally to this work and should be regarded as co-first authors. Citation: Pham M.P., Tran V.H., Vu D.D., Nguyen Q.K., Shah S.N.M. (2021): Phylogenetics of native conifer species in Vietnam based on two chloroplast gene regions rbcL and matK. Czech J. Genet. Plant Breed., 57: 58−66. Abstract: We used two chloroplast gene regions (matK and rbcL) as a tool for the identification of 33 local conifer spe- cies. All 136 sequences, 101 newly generated (14 species for gene matK; 16 species for gene rbcL) and 35 retrieved from the GenBank, were used in the analysis. The highest genetic distance (matK region) was recorded between the species in Cupressaceae with an average of 5% (0.1–8.5), Podocarpaceae with an average of 6% (0–8.5), Taxaceae with an average of 5% (0.2–0.5) and Pinaceae with an average of 20.4% (0.8–54.1).