1 Kl_Komy et al.

Supplementary Table 1. Sequence reads information per each sample Reads information each quality control step Filtered and Denoised Sum of predicted Sample name Reads of raw Merged reads Chimera re- trimmed reads by OTUs counts by file with reverse moved reads filtered reads reads DADA2 PiCRISt2 Sap1 225,040 209,289 207,710 . 173,343 169,995 166,272.9 Sap2 205,481 194,495 194,002 . 174,793 173,781 173,180.6 Sap3 245,906 227,120 225,748 . 188,061 107,386 99,581.74 Rhizosphere1 219,787 203,543 191,859 . 157,845 157,842 147,842 Rhizosphere2 241,335 222,647 214,298 . 191,399 191,388 179,248.9 Rhizosphere3 268,366 247,275 238,479 . 208,358 208,343 193,907.3 Fungi Sap1 291,214 123,725 122,930 113,624 107,675 97,803 . Sap2 269,069 109,586 109,272 97,483 89,828 67,187 . Sap3 292,766 117,861 117,469 103,169 87,178 51,127 . Rhizosphere1 274,281 113,973 112,994 97,887 97,448 84,514 . Rhizosphere2 266,491 103,916 103,132 89,914 89,540 82,156 . Rhizosphere3 268,473 109,050 108,380 93,813 91,784 84,948 . OTU, operational taxonomic unit.

Supplementary Table 2. Common fungal strains in both rhizosphere and endosphere in kiwifruit Representative OTU OTU Phylum Family Accession Abundant OTUs (%) ID Strain number Rhizo Sap F1 Didymellaceae Didymella bellidis MH719233.1 1.84 ± 0.87 16.02 ± 13.85 F3 Ascomycota Phaeosphaeriaceae Paraphoma sp. KR094449.1 7.04 ± 9.62 4.69 ± 0.71 F5 Ascomycota Cladosporiaceae Cladosporium cladosporioides MK813962.1 0.42 ± 0.19 8.13 ± 1.97 F11 Mortierellomycota Mortierellaceae gamsii MH857052.1 3.93 ±1.52 0.28 ± 0.27 F12 Mortierellomycota Mortierellaceae Mortierella elongata MG052958.1 2.44 ± 0.92 1.62 ± 0.95 F24 Piskurozymaceae Solicoccozyma terricola KX359606.1 0.92 ± 0.24 0.69 ± 0.41 F27 Mortierellomycota Mortierellaceae Mortierella sp. JX975932.1 0.81 ± 0.17 0.66 ± 0.37 F31 Ascomycota Chaetomiaceae Trichocladium opacum KF850373.1 0.74 ± 0.24 0.44 ± 0.25 F39 Ascomycota Pseudeurotiaceae Pseudogymnoascus pannorum MH854615.1 0.53 ± 0.29 0.15 ± 0.09 F44 Basidiomycota Clavariaceae Clavaria gibbsiae KP257129.1 0.32 ± 0.12 0.36 ± 0.27 F47 Basidiomycota Trichosporonaceae Apiotrichum dehoogii MH487610.1 0.31 ± 0.10 0.30 ± 0.21 F67 GS01 Not assigned Uncultured KX195309.1 0.27 ± 0.05 0.19 ± 0.17 F89 Ascomycota Helotiaceae Tetracladium sp. HG935221.1 0.15 ± 0.04 0.13 ± 0.06 OTU, operational taxonomic unit.

Supplementary Table 3. Common bacterial strains in both rhizosphere and endosphere Representative OTU OTU ID Phylum Family Accession Abundant OTUs (%) Strain number Rhizo Sap B23 Microbacteriaceae Curtobacterium sp. MK039023.1 0.04 ± 0.01 1.22 ± 0.94 B41 Firmicutes Bacillaceae Bacillus sp. MK602562.1 0.43 ± 0.11 0.07 ± 0.08 B61 Proteobacteria Sphingomonadaceae Sphingomonas sp. MK843306.1 0.06 ± 0.02 0.32 ± 0.23 B63 Proteobacteria Sphingomonadaceae Sphingomonas sp. LR584273.1 0.11 ± 0.03 0.12 ± 0.04 B64 Proteobacteria Sphingomonadaceae Sphingomonas sp. MH769434.1 0.12 ± 0.04 0.32. ± 0.36 B118 Actinobacteria Angustibacter sp. MH698690.1 0.07 ± 0.02 0.15 ± 0.11 B131 Proteobacteria Sphingomonadaceae Novosphingobium sp. MK863544.1 0.05 ± 0.02 0.06 ± 0.04 OTU, operational taxonomic unit. 2 Kl_Komy et al.

Supplementary Fig. 1. Rarefaction curves of sap and rhizosphere samples. Bacterial (A) and fungal (B) rarefaction. OTU, opera- tional taxonomic unit.

Biological Control of Tomato Fusarium Wilt 3

Supplementary Fig. 2. Diversity of kiwifruit microbes in rhizosphere and endosphere. Simpson and Shannon values of were calculated from bacteria (A) and fungi (B) operational taxonomic unit of rhizosphere and endosphere.

Supplementary Fig. 3. Detailed functional profiling of fungi by FUNGuild. 4 Kl_Komy et al.

Supplementary Fig. 4. Kyoto Encyclopedia of Genes and Genomes (KEGG) BRITE mapping results of predicted differential en- zymes. The differential enzymes were analyzed by DESeq2 after enzyme predicted by PiCRUSt2. Vertical axis which means count of KEGG enzyme ID was calibrated to the logarithmic scale.