DOCSLIB.ORG

Establishment of Axenic Explants and Callus Culture of Clinacanthus Nutans (Rumput Belalai Gajah)

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Establishment of Axenic Explants and Callus Culture of Clinacanthus Nutans (Rumput Belalai Gajah) ESTABLISHMENT OF AXENIC EXPLANTS AND CALLUS CULTURE OF CLINACANTHUS NUTANS (RUMPUT BELALAI GAJAH) Ng Li Ying QK 769 NS76 Bachelor of Science with Honours 2013 (Plant Resource Science and Management) 2013 I ----~-~ Pus at Khidmat Maklumat Akademik UNlVERSm MALAYSIA SARAWAK Establishment of Axenic explants and Callus Culture of Clinacanthus nutans (rumput belalai gajah) F'.KHIDMAT MAKLUMAT AKADEMIK 11111 1IIIIfiiiilili III III 1000246800 NG LI YING (27241) This report is submitted in partial fulfilment of the requirement for the degree of Bachelor of Science with Honours in Plant Resource Science and Management Plant Resource Science and Management FACULTY OF RESOURCE SCIENCE & TECHNOLOGY UNIVERSITY MALAYSIA SARA WAK 94300 KOTA SAMARAHAN DECLARATION I declare that this thesis entitle "Establishment of Axenic explants and Callus Culture of Clinacanthus nutans (rumput belalai gajah)" is the result of my own research except as cited in the references. The thesis has not been accepted for any degree and is not concurrently submitted in candidature of any other degree. Signature: ~ Name: lin 11 Y/Nh Date: / . 1-, dO/~ ACKNOWLEDGEMENT Firstly, I would like to dedicate my sincere thanks to my supervisor, Prof. Dr. Hamsawi Sani for giving me the opportunity to work on this project and his intellectual support throughout this study. His generous, sincere guidance and advice enable me to complete this project on time. Nevertheless, my appreciation is extended to my senior and master students from Tissue Culture Laboratory, FRST, UNIMAS: Fong Yin Mei, Chen Mei Yin and Cherrie Lim for their invaluable time and advice to support me in completing my laboratory work in these two semester. Our sweet memories always on my mind forever. Last but not least, my warmest gratitude to my beloved family and my soulmate Chia Choon Hiung. Their continuous support, care, love and understanding have built me to a better person in my campus life. Thanks for being with me always. Pusat Khidmat Maklumat Akademik UNIVERSm MALAYSIA SARAWAK TABLE OF CONTENTS Page ACKNOWLEDGEMENTS TABLE OF CONTENTS ii APPENDICES iv LIST OF ABBREVIATION v LIST OF FIGURES vi LIST OF TABLES viii ABSTRACT/ABSTRAK ix CHAPTER 1 INTRODUCTION 1 CHAPTER 2 LITERATURE REVIEW 4 2.1 The plants 4 2.2 Botanical description 4 2.3 The economic importance of Clinacanthus nutans 3.3.1 Medicinal uses of Clinacanthus spp. .. 8 2.4 Micropropagation ofmedicinal plants 2.4.1 Selection ofexplants ........................................... 11 2.4.2 Establishment ofaxenic explants .......................... ... 12 2.4.3 Plant growth regulator and media............................ 14 2.5 Callus culture............................................................... 16 2.6 Acclimatization.. ............................................ .... ........... 17 ii CHAPTER 3 MATERIALS AND METHODS 3.1 Development of an effective surface sterilization regime for the explants ................................................................. .... 18 3.2 Establishment of axenic explant .... .................................... 20 3.3 Induction of callus formation through indirect organogenesis. ..... 21 3.4 Shoot regeneration from callus tissue ............. .. ................... 23 CHAPTER 4 RESULTS AND DISCUSSION 4.1 Establishment ofaxenic explant 4.1 .1 Effect of different sodium hypochlorite (Clorox®) concentration ................................................... 25 4.1.2 Effect of Sodium hyochlorite (Clorox®) concentrations with benomyl ............................ :.... 32 4.2 Induction of callus fonnation through indirect organogenesis 4.2.1 Induction of callus fonnation using 2,4-D..... .............. 34 4.2.2 Induction of callus fonnation using Picloram ............... 35 4.2.3 Induction of callus fonnation using BAP ................. ... 36 4.3 Shoot regeneration from callus tissue 4.3.1 Shoot regeneration by BAP and in combination with IBA .......... ............... ........ 41 CHAPTERS CONCLUSION AND RECOMMENDATION 43 REFERENCES iii APPENDICES Page Appendix A Murashige and Skoog (1962) medium fonnulation 51 Appendix B Number of axenic, contaminated and damaged leaf 52 explants in half MS soft agar after surface sterilization in 10% of Clorox® concentration at day 14 after initiation of culture Appendix C Number of axenic, contaminated and damaged internode 53 explants in half MS soft agar after surface sterilization in 10% of Clorox® concentration at day 14 after initiation of culture Appendix D Number of axenic, contaminated and damaged shoot tip 54 explants in half MS soft agar after surface sterilization in 10% of Clorox® concentration at day 14 after initiation of culture Appendix E Number of axenic, contaminated and damaged leaf explants 55 in half MS soft 'agar after surface sterilization in 15% of Clorox® concentration at day 14 after initiation of culture Appendix F Number of axenic, contaminated and damaged internode 56 explants in half MS soft agar after surface sterilization in 15% of Clorox® concentration at day 14 after initiation of culture Appendix G Number of axenic, contaminated and damaged shoot tip 57 explants in half MS soft agar after surface sterilization in 15% of Clorox® concentration at day 14 after initiation of culture iv LIST OF ABBREVATIONS BAP 6-benzyl amino purine Benomyl Methyl [l-[(butylamino )carbonyl]-l H-benzimidazol-2-yl]carbamate B5 Gamborg B5 medium rnA Indole-3-butyric acid MS Murashige and Skoog medium NAA Naphthalene acetic acid Picloram 4 Amino-2,5,6-trichloropicolinic acid PPMTM Plant preservative mixture WPM Wood Plant Medium 2,4- D 2,4- dichlorophenoxyacetic acids v LIST OF FIGURES Figure No. Page 2.1 The Snake Grass (Clinacanthus nutans) leaves 6 2.2 The Snake Grass (Clinacanthus nutans) seedling 7 4.1 Percentage of axenic explants with response to 10% Clorox® 26 concentration in 10 minutes after 14 days 4.2 (a) Axenic leaf explants after culturing for 14 days in halfMS 27 medium (b) Fluffy and whitish callus formed at the cut edge of internode explants after culturing for 14 days in half MS medium (c) Axenic shoot tip explants after culturing for 14 days in half MS medium (d) Damaged leafexplants after culturing for 14 days in halfMS medium 4.3 Percentage ofaxenic explants with response to 15% Clorox® 29 concentration in 10 minutes after 14 days 4.4 (a) Leaf explants contaminated with pinkish bacteria 31 (b) Internode explants contaminated with whitish fungi (c) Shoot tip explants contaminated with whitish fungi and bacteria 4.5 Comparison between percentage of axenic explants with response 32 to 10% Clorox® concentration with and without the addition of 0.1 % of Benomyl fungicide in 10 minutes after 14 days 4.6 (a) Internode without any sign of regeneration in MS medium 35 supplemented with different concentration of2,4-D (b) Shrunk and yellowish brown callus formed at the cut edges of internode explant 4.7 Percentage of internode explants producing callus with response 37 to different BAP concentration after 2 months of cultures vi 4.8 (a) A light callusing fonned at the cut edges of internode explant 39 after one week of culturing in MS medium supplemented with 0.5 mg/l BAP (b) A light callusing fonned at the cut edges of internode explant after one week of culturing in MS medium supplemented with 1.0 mg/l BAP (c) Creamy white and fluffy callus fonned at the cut edges of internode explant after four weeks ofculturing in MS medium supplemented with 1.5 mg/l BAP (d) Creamy white and fluffy callus fonned at the cut edges of internode explant after four week of culturing in MS medium supplemented with 2.0 mg/l BAP 4.9 (a) Yellowish brown callus fonned at the cut edges of internode explant 40 after 2 months of culturing in MS medium supplemented with 0.5 mgll BAP (b ) Yellowish brown callus formed at the cut edges of internode explant after 2 months of culturing in MS medium supplemented with 1.0 mg/l BAP (c) Creamy white and fluffy callus fonned at the cut edges of internode explant after 2 months of culturing in MS medium supplemented with 2.0 mg/l BAP (d) Creamy white and fluffy callus fonned at the cut edges of internode explant after 2 months ofculturing in MS medium supplemented with 2.0 mg/l BAP 4.10 (a) No any sign of shoot regenerated from the callus derived from explant 42 after two weeks of culturing in MS medium supplemented with 2.0 mg/l BAP and 1.0mg/l IBA (b) Died callus without any shoot induced after four weeks of culturing in MS medium supplemented with 2.0 mg/l BAP and 1.Omg/l IBA vii LIST OF TABLES Tables No. Page 4.1 Chi-squared comparison between different types ofexplants 28 with response to 10% Clorox® concentration 4.2 Chi-squared comparison between different types ofexplants 30 with response to 15% Clorox® concentration 4.3 Chi-squared comparison between different BAP on 38 explants producing callus viii Establishment of Axenic explants and Callus Culture of Clinacanthus nutans (rumput belalai gajah) Ng Li Ying Plant Resource and Management Programme Faculty of Science and Technology Universiti Malaysia Sarawak ABSTRACT Clinacanthus nulans (Burm.f.) Lindau (snake grass) of family Acanthaceae is known as a herb, shrub or ornamental grass used for medicinal purposes. Clinacanthus nlltans is a famous tropical herb and have been used widely in Southeast Asia especially in Sabah, Malaysia as an anti- inflammatory drug for the treatment of wound, disinfection and cancer. The purpose of this study is to establish an effective surface sterilization protocol and to develop an in vitro propagation of Clinacanthlls nutans using plant hormone. The study started with the establishment of axenic explants culture. Shoot tips, internode and leaves from grown seedling of snake grass were as the ex plants. Resulted showed most of the explants were found damaged by treatment of 15% 'Clorox®' . The protocol below would result in 93% contamination-free internodes explant in culture. The protocol involves dipping explants first in 70% ethanol for 30 seconds followed by agitating the explants in 10% 'Clorox®' for 10 minutes, rinsed at least three times with distilled water and then dipped in 0.1% of benomyl fungicide for one hour, rinsed for three times before culture in half MS medium.
Load more

Recommended publications
  • Acanthaceae), a New Chinese Endemic Genus Segregated from Justicia (Acanthaceae)
    Plant Diversity xxx (2016) 1e10 Contents lists available at ScienceDirect Plant Diversity journal homepage: http://www.keaipublishing.com/en/journals/plant-diversity/ http://journal.kib.ac.cn Wuacanthus (Acanthaceae), a new Chinese endemic genus segregated from Justicia (Acanthaceae) * Yunfei Deng a, , Chunming Gao b, Nianhe Xia a, Hua Peng c a Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, People's Republic of China b Shandong Provincial Engineering and Technology Research Center for Wild Plant Resources Development and Application of Yellow River Delta, Facultyof Life Science, Binzhou University, Binzhou, 256603, Shandong, People's Republic of China c Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, People's Republic of China article info abstract Article history: A new genus, Wuacanthus Y.F. Deng, N.H. Xia & H. Peng (Acanthaceae), is described from the Hengduan Received 30 September 2016 Mountains, China. Wuacanthus is based on Wuacanthus microdontus (W.W.Sm.) Y.F. Deng, N.H. Xia & H. Received in revised form Peng, originally published in Justicia and then moved to Mananthes. The new genus is characterized by its 25 November 2016 shrub habit, strongly 2-lipped corolla, the 2-lobed upper lip, 3-lobed lower lip, 2 stamens, bithecous Accepted 25 November 2016 anthers, parallel thecae with two spurs at the base, 2 ovules in each locule, and the 4-seeded capsule. Available online xxx Phylogenetic analyses show that the new genus belongs to the Pseuderanthemum lineage in tribe Justi- cieae.
    [Show full text]
  • Lamiales – Synoptical Classification Vers
    Lamiales – Synoptical classification vers. 2.6.2 (in prog.) Updated: 12 April, 2016 A Synoptical Classification of the Lamiales Version 2.6.2 (This is a working document) Compiled by Richard Olmstead With the help of: D. Albach, P. Beardsley, D. Bedigian, B. Bremer, P. Cantino, J. Chau, J. L. Clark, B. Drew, P. Garnock- Jones, S. Grose (Heydler), R. Harley, H.-D. Ihlenfeldt, B. Li, L. Lohmann, S. Mathews, L. McDade, K. Müller, E. Norman, N. O’Leary, B. Oxelman, J. Reveal, R. Scotland, J. Smith, D. Tank, E. Tripp, S. Wagstaff, E. Wallander, A. Weber, A. Wolfe, A. Wortley, N. Young, M. Zjhra, and many others [estimated 25 families, 1041 genera, and ca. 21,878 species in Lamiales] The goal of this project is to produce a working infraordinal classification of the Lamiales to genus with information on distribution and species richness. All recognized taxa will be clades; adherence to Linnaean ranks is optional. Synonymy is very incomplete (comprehensive synonymy is not a goal of the project, but could be incorporated). Although I anticipate producing a publishable version of this classification at a future date, my near- term goal is to produce a web-accessible version, which will be available to the public and which will be updated regularly through input from systematists familiar with taxa within the Lamiales. For further information on the project and to provide information for future versions, please contact R. Olmstead via email at [email protected], or by regular mail at: Department of Biology, Box 355325, University of Washington, Seattle WA 98195, USA.
    [Show full text]
  • Should DNA Sequence Be Incorporated with Other Taxonomical Data for Routine Identifying of Plant Species?
    Suesatpanit et al. BMC Complementary and Alternative Medicine (2017) 17:437 DOI 10.1186/s12906-017-1937-3 RESEARCHARTICLE Open Access Should DNA sequence be incorporated with other taxonomical data for routine identifying of plant species? Tanakorn Suesatpanit1, Kitisak Osathanunkul2, Panagiotis Madesis3 and Maslin Osathanunkul1,4* Abstract Background: A variety of plants in Acanthaceae have long been used in traditional Thai ailment and commercialised with significant economic value. Nowadays medicinal plants are sold in processed forms and thus morphological authentication is almost impossible. Full identification requires comparison of the specimen with some authoritative sources, such as a full and accurate description and verification of the species deposited in herbarium. Intake of wrong herbals can cause adverse effects. Identification of both raw materials and end products is therefore needed. Methods: Here, the potential of a DNA-based identification method, called Bar-HRM (DNA barcoding coupled with High Resolution Melting analysis), in raw material species identification is investigated. DNA barcode sequences from five regions (matK, rbcL, trnH-psbA spacer region, trnL and ITS2) of Acanthaceae species were retrieved for in silico analysis. Then the specific primer pairs were used in HRM assay to generate unique melting profiles for each plants species. Results: The method allows identification of samples lacking necessary morphological parts. In silico analyses of all five selected regions suggested that ITS2 is the most suitable marker for Bar-HRM in this study. The HRM analysis on dried samples of 16 Acanthaceae medicinal species was then performed using primer pair derived from ITS2 region. 100% discrimination of the tested samples at both genus and species level was observed.
    [Show full text]
  • Antimicrobial Effect of Medicinal Plants Used in Traditional Indonesian Medicine
    CZECH UNIVERSITY OF LIFE SCIENCES PRAGUE Faculty of Tropical AgriSciences Department of Crop Sciences and Agroforestry Antimicrobial effect of medicinal plants used in traditional Indonesian medicine DOCTORAL THESIS Author: Andreas Romulo Supervisor: prof. Ing. Ladislav Kokoška, Ph.D. 2018 ACKNOWLEDGMENT I would like to express my gratitude towards my exceptional supervisor prof. Ladislav Kokoška, Ph.D. for his sincere guidance, encouragement, and patience over the last three years. Thank you for giving me the opportunity to do research in the Laboratory of Ethnobotany and Ethnopharmacology. Your continuous support, insightful comments, suggestion, and critical analysis of the research and writing of this dissertation were essential for completion of my study and the success of my future career. I would like to thank Ing. Johana Rondevaldová, Ph.D. for the support and helpful assistance in laboratory research and academic background, as well as to my fellow labmates, Ing. Markéta Houdková and Ing. Marie Netopilová who helped and contributed in the experiment. My special thanks to Ing. Klára Urbanová, Ph.D. and Ing. Pavel Nový, Ph.D. for their sincere help, guidance, and suggestion during the analysis of the essential oil. Finally, I would like to thank to my beloved parents and friends for their continues support, encouragement, and pray. I am so grateful to have you all. This research was financially supported by Erasmus Mundus project ALFABET (Asia: Life, Food, Agriculture, Biology, Economics, Technology) [55207], Czech University of Life Sciences Prague Grant Agency project CIGA [20175001] and Internal Grant Agency project IGA [20175020]. II CERTIFICATION I, Andreas Romulo, submitted this dissertation for Ph.D.
    [Show full text]
  • Clinacanthus Nutans (Burm
    Available online on www.ijppr.com International Journal of Pharmacognosy and Phytochemical Research 2015; 7(6); 1244-1250 ISSN: 0975-4873 Research Article Clinacanthus nutans (burm. F.) Lindau: An Useful Medicinal Plant of South-East Asia Ruhaiyem Yahaya*, Gouri Kumar Dash, Mohd. Syafiq Abdullah and Allan Mathews Faculty of Pharmacy and Health Sciences, Universiti Kuala Lumpur Royal College of Medicine Perak, 30450 Ipoh, Perak, Malaysia. Available Online:30th November, 2015 ABSTRACT The present paper outlines the traditional use, pharmacological and phytochemical aspects of a very popular medicinal plant Clinacanthus nutans (Burm. f.) Lindau (Family-Acanthaceae), primarily used in South East Asian countries. Owing to its antiviral properties, the extracts of this plant is used in many hospitals in Thailand for treating herpes simplex virus and herpes zoster virus. The plant has been included in ‘Thai Herbal National Essential Drug List’ and promoted for the treatment of herpes simplex, herpes zoster, and skin pruritis in primary health care programmes. A complete review involving the traditional uses, phytochemical constituents and pharmacological properties of C. nutans has been made using published papers, databases and books. The plant contains terpenoids, flavonoids, C-glycosyl flavones, sulfur- containing glucosides and glycoglycerolipids which contribute to the pharmacological activities. Pharmacological studies revealed that C. nutans possesses antiviral, anticancer, antioxidant, antidiabetic, immunomodulatory, wound healing, anti-inflammatory and analgesic activities. Clinical trials of the creams containing extracts of C. nutans in the treatment of Herpes genitalis and Herpes zoster infection showed successful results. Few isolated compounds are reported to possess anti-herpes simplex activity. Acute and subacute toxicity studies revealed C.
    [Show full text]
  • Medicinal Plant Research Volume 8 Number 25, 3 July, 2014 ISSN 2009-9723
    Journal of Medicinal Plant Research Volume 8 Number 25, 3 July, 2014 ISSN 2009-9723 ABOUT JMPR The Journal of Medicinal Plant Research is published weekly (one volume per year) by Academic Journals. The Journal of Medicinal Plants Research (JMPR) is an open access journal that provides rapid publication (weekly) of articles in all areas of Medicinal Plants research, Ethnopharmacology, Fitoterapia, Phytomedicine etc. The Journal welcomes the submission of manuscripts that meet the general criteria of significance and scientific excellence. Papers will be published shortly after acceptance. All articles published in JMPR are peerreviewed. Electronic submission of manuscripts is strongly encouraged, provided that the text, tables, and figures are included in a single Microsoft Word file (preferably in Arial font). Submission of Manuscript Submit manuscripts as e-mail attachment to the Editorial Office at: [email protected]. A manuscript number will be mailed to the corresponding author shortly after submission. The Journal of Medicinal Plant Research will only accept manuscripts submitted as e-mail attachments. Please read the Instructions for Authors before submitting your manuscript. The manuscript files should be given the last name of the first author. Editors Prof. Akah Peter Achunike Prof. Parveen Bansal Editor-in-chief Department of Biochemistry Department of Pharmacology & Toxicology Postgraduate Institute of Medical Education and University of Nigeria, Nsukka Research Nigeria Chandigarh India. Associate Editors Dr. Ravichandran Veerasamy AIMST University Dr. Ugur Cakilcioglu Faculty of Pharmacy, AIMST University, Semeling - Elazıg Directorate of National Education 08100, Turkey. Kedah, Malaysia. Dr. Jianxin Chen Dr. Sayeed Ahmad Information Center, Herbal Medicine Laboratory, Department of Beijing University of Chinese Medicine, Pharmacognosy and Phytochemistry, Beijing, China Faculty of Pharmacy, Jamia Hamdard (Hamdard 100029, University), Hamdard Nagar, New Delhi, 110062, China.
    [Show full text]
  • The Potencial Effect of Dandang Gendis Leave (Clinacanthus Nutans
    2nd International Conference of Health, Science and Technology 2021 ISBN: 978-623-92207-1-6 Literature Review: The Potencial Effect of Dandang Gendis Leave (Clinacanthus nutans Lindau) 1st Kusumaningtyas Siwi Artini 2nd Weri Veranita 3rd Tatiana Siska Wardani Pharmacy Department, Faculty of Pharmacy Department, Faculty of Pharmacy Department, Faculty of Health Science Health Science Health Science Duta Bangsa University Duta Bangsa University Duta Bangsa University Surakarta, Indonesia Surakarta, Indonesia Surakarta, Indonesia [email protected] [email protected] [email protected] Abstract—Indonesian are used to use herb to medicate a following is the classification and nomenclature of disease. One of the medicinal plants used is Clinacnathus nutans Clinacanthus nutans Lindau: (Burm.f.) Lindau. Clinacnathus nutans Lindau is a traditional Kingdom: Plantae Phylum: Magnoliophyta plant, commonly known as dandang gendhis (Java – Class: Magnoliopsida Indonesia), ki tajam (Sunda – Indonesia), Belalai gajah Order: Lamiales (Malaysia), SaBah Snake Grass (Singapore), and phaya Family: Acanthaceae yo (Thailand). It belonging to the Acanthaceae family. This Genus: Clinacanthus study aimed to find out the the effect of giving the extract of Species: nutans- Lindau Scientific Clinacnathus nutans Lindau to subject. This research method Name: Clinacanthus nutans (Burm. f.) Lindau was using literature review. The information about The stem is smooth, the leaves are simple, narrow Clinacanthus nutans Lindau collected from an online database oval or lanceolate with a length of 2.5 - 13 cm and a width of such as Pub Med, Scient direct and Google Scholar. The number 0.5 - 1.5 cm with a sharp apex, the base of the leaves is round of chemical compound were identified from leaves.
    [Show full text]
  • Medicinal Plants Research in Asia, Volume 1
    i MEDICINAL PLANTS RESEARCH IN ASIA, VOLUME 1 The International Plant Genetic Resources Institute (IPGRI) is an independent international scientific organization that seeks to advance the conservation and use of plant genetic diversity for the well-being of present and future generations. It is one of 15 Future Harvest Centres supported by the Consultative Group on International Agricultural Research (CGIAR), an association of public and private members who support efforts to mobilize cutting-edge science to reduce hunger and poverty, improve human nutrition and health, and protect the environment. IPGRI has its headquarters in Maccarese, near Rome, Italy, with offices in more than 20 other countries worldwide. The Institute operates through three programmes: (1) the Plant Genetic Resources Programme, (2) the CGIAR Genetic Resources Support Programme, and (3) the International Network for the Improvement of Banana and Plantain (INIBAP). The international status of IPGRI is conferred under an Establishment Agreement which, by January 2003, had been signed by the Governments of Algeria, Australia, Belgium, Benin, Bolivia, Brazil, Burkina Faso, Cameroon, Chile, China, Congo, Costa Rica, Côte d’Ivoire, Cyprus, Czech Republic, Denmark, Ecuador, Egypt, Greece, Guinea, Hungary, India, Indonesia, Iran, Israel, Italy, Jordan, Kenya, Malaysia, Mauritania, Morocco, Norway, Pakistan, Panama, Peru, Poland, Portugal, Romania, Russia, Senegal, Slovakia, Sudan, Switzerland, Syria, Tunisia, Turkey, Uganda and Ukraine. Financial support for IPGRI’s research is provided by more than 150 donors, including governments, private foundations and international organizations. For details of donors and research activities please see IPGRI’s Annual Reports, which are available in printed form on request from [email protected] or from IPGRI’s web site (www.ipgri.cgiar.org).
    [Show full text]
  • A Review of Therapeutic Potentials of Clinacanthus Nutans As Source For
    Sains Malaysiana 48(12)(2019): 2683–2691 http://dx.doi.org/10.17576/jsm-2019-4812-09 A Review of Therapeutic Potentials of Clinacanthus nutans as Source for Alternative Medicines (Ulasan Potensi Terapeutik Clinacanthus nutans sebagai Punca Perubatan Alternatif) ZAINOL HAIDA & MANSOR HAKIMAN* ABSTRACT Clinacanthus nutans, also known as Sabah Snake Grass, is an ethno-medicinal plant species belonging to the family Acanthaceae and has been widely used as a traditional remedy in Malaysia and Thailand to treat various diseases. The species contains massive amount of phytochemical compounds useful to human health. Various studies have reported that among the compounds found in the species are those with anti-venom properties reported to be rarely present in other medicinal plants. A number of pharmacological research studies have also documented that extracts of C. nutans contain high antioxidants, as well as compounds with anti-cancer, anti-inflammatory and anti-viral properties. Isolation and identification of these phytochemical compounds have gained global attention due to their well-known beneficial values. The present review article is a compilation of previous pharmacological studies on C. nutans with a view of gaining further interest in exploring alternative sources of medicine. Keywords: Clinacanthus nutans; medicinal plant; pharmacological constituents ABSTRAK Clinacanthus nutans atau dikenali sebagai Belalai gajah, ialah tumbuhan ubatan daripada famili Acanthaceae. C. nutans digunakan secara meluas di Malaysia dan Thailand dalam perubatan tradisi untuk merawat pelbagai jenis penyakit. C. nutans mempunyai kandungan fitokimia yang tinggi. Justeru, dapat memberi manfaat kepada manusia. Antara semua potensi terapeutik yang terdapat dalam C. nutans, anti bisa merupakan kelebihan pokok ini.
    [Show full text]
  • BOTANICAL ILLUSTRATION WORKSHOP 1-2 September 2013
    CONTENT CONTENT WELCOME MESSAGE ............................................................................................... 1 ORGANIZING COMMITTEE ...................................................................................... 2 GENERAL INFORMATION ....................................................................................... 5 CONFERENCE PROGRAM OVERVIEW ............................................................ 12 KEYNOTE LECTURE ................................................................................................. 16 ORAL PRESENTATION DAY 1: SESSION 1 – 2 ........................................................................................ 19 DAY 2: SESSION 3 – 6 ........................................................................................ 54 DAY 4: SESSION 7 – 9 ........................................................................................ 126 DAY 5: SESSION 10 – 12 .................................................................................... 178 POSTER PRESENTATION ......................................................................................... 217 MISCELLANEOUS ....................................................................................................... 295 LIST OF PARTICIPANTS .......................................................................................... 304 AUTHOR INDEX ........................................................................................................ 329 9th International Flora Malesiana Symposium i WELCOME MESSAGE
    [Show full text]
  • Studies on Clinacanthus Nutans
    Genetic, phytochemical and bioactivity studies of Clinacanthus nutans (Burm. f.) Lindau (Acanthaceae) A thesis submitted in fulfilment of the requirements for the degree of Doctor of Philosophy (Applied Biology and Biotechnology) Siat Yee Fong BSc (Biotechnology) (Monash), MAppSc (Molecular Biotechnology) (USYD) School of Applied Sciences College of Science, Engineering and Health RMIT University August 2015 Declaration I certify that except where due acknowledgement has been made, the work is that of the author alone; the work has not been submitted previously, in whole or in part, to qualify for any other academic award; the content of the thesis is the result of work which has been carried out since the official commencement date of the approved research program; any editorial work, paid or unpaid, carried out by a third party is acknowledged; and, ethics procedures and guidelines have been followed. Siat Yee Fong 31 August 2015 i Acknowledgements I thank my supervisors, Dr Tien Huynh, Associate Professor Terrence Piva and Dr Sylvia Urban for their supervision, advice, encouragement, help and support during my entire PhD tenure. Thank you for your insight and wisdom in manuscripts and thesis. I am also grateful to Professor Ann Lawrie, whose advice has been generously given on a number of occasions. I acknowledge TKC farm, Kui Min Liau, Vun Jhit Lim and Rattanapong Charntawong for the provision of plant materials. My sincere thanks to Robert Brkljaca and Dr Chaitali Dekiwadia for their help in HPLC and TEM work, respectively. Thanks are also extended to Layla Mehdi Alhasan, Griff D’Costa, Xian Yang Chan, Alaa Banjar and Sushil Talda for providing the cells and reagents used in this study.
    [Show full text]
  • Download Download
    Innovare International Journal of Pharmacy and Pharmaceutical Sciences Academic Sciences ISSN- 0975-1491 Vol 7, Issue 2, 2015 Review Article A REVIEW ON PHYTOCHEMICAL CONSTITUENTS AND PHARMACOLOGICAL ACTIVITIES OF CLINACANTHUS NUTANS MUHAMMAD SHAHZAD ASLAM*, MUHAMMAD SYARHABIL AHMAD, AWANG SOH MAMAT School of Bioprocess Engineering, University Malaysia Perlis, Kompleks Pusat Pengajian, Jejawi 3, 02600 Arau, Perlis, Malaysia. Email: [email protected] Received: 14 Aug 2014 Revised and Accepted: 15 Sep 2014 ABSTRACT Clinacanthus nutans commonly known as Belalai gajah (Malay), Phaya yo (Thai) is traditionally used medicinal plant. The plant is used in skin rashes, snake bites, lesions caused by herpes simplex virus, diabetic myelitis, fever, diuretics and green tea and served as fresh drink. The phytochemical constituents existing in the plant comprise lupeol, b –sitosterol, stigmasterol, Botulin, myricyl alcohol C-glycosyl flavones (vitexin, isovitexin, shaftoside, isomollupentin 7-O-b -glucopyranoside, orientin and isoorientin),sulfur-containing glucosides, cerebrosides mixer, a monoacylmonogalactosylglycerol,13-hydroxy-(13-S)-phaeophytin b, Pupurin-18-phytyl ester, phaeophorbide and chlorophyll derivatives. Pharmacological studies reported anti-Papillomavirus Infectivity, anti-viral activity on varicella-zoster virus, anti-inflammatory activity, anti-herpes Simplex virus type 1 and type 2 activity, anti-oxidant and protective effect against oxidative induced hemolysis. Keywords: Clinacanthus nutans , Anti-viral, Anti-oxidant, Glycosides, Herpes Simplex Virus. INTRODUCTION Traditional uses Clinacanthus nutans (CN) have been used in traditional medicine in In Malaysia, the fresh leaves are boiled with water and consumed as Thailand and Malaysia. Its therapeutic potential has not been herbal tea. It is used for treating skin rashes and snake bites, lesions explored completely.
    [Show full text]