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Pepsin Homologues in Bacteria Neil D Rawlings* and Alex Bateman

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Pepsin Homologues in Bacteria Neil D Rawlings* and Alex Bateman BMC Genomics BioMed Central Research article Open Access Pepsin homologues in bacteria Neil D Rawlings* and Alex Bateman Address: Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, CB10 1SA, UK Email: Neil D Rawlings* - [email protected]; Alex Bateman - [email protected] * Corresponding author Published: 16 September 2009 Received: 28 November 2008 Accepted: 16 September 2009 BMC Genomics 2009, 10:437 doi:10.1186/1471-2164-10-437 This article is available from: http://www.biomedcentral.com/1471-2164/10/437 © 2009 Rawlings and Bateman; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Peptidase family A1, to which pepsin belongs, had been assumed to be restricted to eukaryotes. The tertiary structure of pepsin shows two lobes with similar folds and it has been suggested that the gene has arisen from an ancient duplication and fusion event. The only sequence similarity between the lobes is restricted to the motif around the active site aspartate and a hydrophobic-hydrophobic-Gly motif. Together, these contribute to an essential structural feature known as a psi-loop. There is one such psi-loop in each lobe, and so each lobe presents an active Asp. The human immunodeficiency virus peptidase, retropepsin, from peptidase family A2 also has a similar fold but consists of one lobe only and has to dimerize to be active. All known members of family A1 show the bilobed structure, but it is unclear if the ancestor of family A1 was similar to an A2 peptidase, or if the ancestral retropepsin was derived from a half-pepsin gene. The presence of a pepsin homologue in a prokaryote might give insights into the evolution of the pepsin family. Results: Homologues of the aspartic peptidase pepsin have been found in the completed genomic sequences from seven species of bacteria. The bacterial homologues, unlike those from eukaryotes, do not possess signal peptides, and would therefore be intracellular acting at neutral pH. The bacterial homologues have Thr218 replaced by Asp, a change which in renin has been shown to confer activity at neutral pH. No pepsin homologues could be detected in any archaean genome. Conclusion: The peptidase family A1 is found in some species of bacteria as well as eukaryotes. The bacterial homologues fall into two groups, one from oceanic bacteria and one from plant symbionts. The bacterial homologues are all predicted to be intracellular proteins, unlike the eukaryotic enzymes. The bacterial homologues are bilobed like pepsin, implying that if no horizontal gene transfer has occurred the duplication and fusion event might be very ancient indeed, preceding the divergence of bacteria and eukaryotes. It is unclear whether all the bacterial homologues are derived from horizontal gene transfer, but those from the plant symbionts probably are. The homologues from oceanic bacteria are most closely related to memapsins (or BACE-1 and BACE-2), but are so divergent that they are close to the root of the phylogenetic tree and to the division of the A1 family into two subfamilies. Background hydrolyse proteins to amino acids and peptides for nutri- Peptidases are widespread enzymes that catalyse the tion and recycling, but they also perform some of the hydrolysis of peptide bonds. Not only do peptidases most important post-translational processing events lead- Page 1 of 10 (page number not for citation purposes) BMC Genomics 2009, 10:437 http://www.biomedcentral.com/1471-2164/10/437 ing to the activation (or inactivation) of many other pro- because, apart from motifs contributing to each psi-loop, teins, including other enzymes and peptide hormones. there is no observable similarity in the protein sequence. Over 2% of the protein coding genes in a genome encode This led to the hypothesis that an ancestral gene duplica- peptidases, and there are over 500 peptidase genes in the tion had taken place, followed by gene fusion [9,9]. This human genome. Peptidases exist in at least six catalytic raises the question of how did the original gene product types, depending on the nature of the nucleophile in the function, being only one half of a modern-day pepsin? catalytic reaction (either the hydroxyl of a serine or threo- The discovery of a peptidase, retropepsin (EC 3.4.23.16), nine, the thiol of a cysteine, or activated water bound within the genome of the human immunodeficiency virus either to a metal ion (metallopeptidases) or aspartate or (HIV) that also represents a single lobe of an extremely glutamate residues). Peptidases are grouped into over 250 divergent pepsin (so divergent that it is the representative different evolutionary families [1]. of a different family, A2, in MEROPS, but within the same clan or superfamily AA) and which has to dimerize to be The pepsin family (MEROPS family A1 [2]) contains active, provided evidence to answer this question [10]. aspartic-type endopeptidases. Activated water is bound by Proteins with a single pepsin lobe also exist in eukaryotes. two aspartate residues each of which is located in a motif The structure of the Saccharomyces cerevisiae protein Ddi1 Xaa-Xaa-Asp-Xbb-Gly-Xbb where Xaa is a hydrophobic (DNA-damage inducible protein 1), which is not a pepti- amino acid and Xbb is either Ser or Thr. A third important dase, has been resolved and shown to contain a domain residue is a tyrosine which interacts with the substrate and with a retropepsin-like structure, and the protein also has is located on a beta-hairpin loop known as the "flap" to dimerize to be active [11]. This domain was probably which forms part of the roof to the active site. A second derived from a retroelement and represents an even more motif, part of a structure known as the psi-loop [3] con- ancient divergence than that of the A1 and A2 families, as sists of two hydrophobic residues followed by a glycine. has been shown in a recent phylogeny of clan AA pepti- Two of these motifs are conserved in all sequences known dases [12]. It has been assumed that the HIV peptidase or predicted to be active within family A1. The psi-loops represents the ancestral state and that the ancestral half- are important in stabilizing the structure, especially the pepsin also had to dimerize to be active. However, it is active centre. Many members of family A1 have three con- also possible that the HIV peptidase was derived from a served disulphide bridges. Homologues are known from normal, bilobed pepsin when a virus captured half of its animals, plants, fungi and protozoa, but no homologues host's gene. This "chicken and egg" situation might be have been reported from prokaryotes. Family A1 has resolvable if pepsin homologues could be found in recently been divided into two subfamilies, with the prokaryotes, because this would represent an extremely majority of characterized peptidases in subfamily A1A. distant sequence divergence. If a prokaryote pepsin Peptidases within this subfamily include: pepsin A (EC homologue contained only one lobe, then it would sup- 3.4.23.1), gastricsin (EC 3.4.23.3) and chymosin (EC port the hypothesis of the ancient gene duplication and 3.4.23.4), which are digestive enzymes in the stomach; fusion event. However, if the prokaryote pepsin homo- cathepsin D (EC 3.4.23.5), which is a lysosomal enzyme logue were bilobed, then either a) this hypothesis is incor- that degrades phagocytozed peptides and proteins; renin rect; b) the gene duplication precedes the most recent (EC 3.4.23.15), which is a peptidase that processes angi- common ancestor of prokaryotes and eukaryotes; or c) the otensinogen; and memapsins 1 (or BACE-2, EC bacterial homologue genes were derived by horizontal 3.4.23.45) and 2 (BACE-1, EC 3.4.23.46), widely distrib- gene transfer. uted peptidases of unknown function but which may be involved in Alzheimer's disease (memapsin-2 is currently We report the discovery of the first prokaryote pepsin under investigation as a drug target [4]). Subfamily A1B homologues in the completed genome sequences of sev- contains predominantly peptidase homologues from eral proteobacteria. plants including nucellin [5] and nepenthesin (EC 3.4.23.12; [6]). Besides peptidases, family A1 also con- Results and Discussion tains a number of proteins which cannot be peptidases Over 960 completely sequenced prokaryote genomes because the active site aspartates are not conserved. These were analysed (914 bacterial and 55 archaean), and 953 include pregnancy-associated glycoproteins [7] and a of these contained no detectable homologue of pepsin. xylanase inhibitor [8]. This included all of the archaean genomes. However, pep- sin homologues were detected in seven bacterial genomes, The tertiary structures of several members of this family and these are shown in Table 1. Characteristics of the pre- have been determined, and each shows a bilobed protein. dicted proteins of these homologues are shown in Table 2. Each lobe contains one of the active site aspartates in a All the bacteria containing pepsin homologues are mem- psi-loop and the two lobes are structurally similar. This bers of the class Gammaproteobacteria, and all except similarity can only be observed in the tertiary structure Marinomonas are members of the order Alteromonadales; Page 2 of 10 (page number not for citation purposes) BMC Genomics 2009, 10:437 http://www.biomedcentral.com/1471-2164/10/437 Table 1: Homologues of pepsin from completely sequenced bacterial genomes Species Genome accession ProteinID Gene locus Base pairs Colwellia psychrerythraea CP000083 AAZ24813 CPS_0549 c(546351..547676) Marinomonas sp.
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