The Hybrid Motor in Shewanella Oneidensis MR-1
Total Page:16
File Type:pdf, Size:1020Kb
Load more
Recommended publications
-
Shewanella Oneidensis MR-1 Nanowires Are Outer Membrane and Periplasmic Extensions of the Extracellular Electron Transport Components
Shewanella oneidensis MR-1 nanowires are outer membrane and periplasmic extensions of the extracellular electron transport components Sahand Pirbadiana, Sarah E. Barchingerb, Kar Man Leunga, Hye Suk Byuna, Yamini Jangira, Rachida A. Bouhennic, Samantha B. Reedd, Margaret F. Romined, Daad A. Saffarinic, Liang Shid, Yuri A. Gorbye, John H. Golbeckb,f, and Mohamed Y. El-Naggara,g,1 aDepartment of Physics and Astronomy, University of Southern California, Los Angeles, CA 90089; bDepartment of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16802; cDepartment of Biological Sciences, University of Wisconsin, Milwaukee, WI 53211; dPacific Northwest National Laboratory, Richland, WA 99354; eDepartment of Civil and Environmental Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180; fDepartment of Chemistry, Pennsylvania State University, University Park, PA 16802; and gMolecular and Computational Biology Section, Department of Biological Sciences, University of Southern California, Los Angeles, CA 90089 Edited by Harry B. Gray, California Institute of Technology, Pasadena, CA, and approved July 30, 2014 (received for review June 9, 2014) Bacterial nanowires offer an extracellular electron transport (EET) or outer membrane cytochromes, and multicellular bacterial pathway for linking the respiratory chain of bacteria to external cables that couple distant redox processes in marine sediments surfaces, including oxidized metals in the environment and (6–13). Functionally, bacterial nanowires are thought to offer an engineered electrodes in renewable energy devices. Despite the extracellular electron transport (EET) pathway linking metal- global, environmental, and technological consequences of this reducing bacteria, including Shewanella and Geobacter species, to biotic–abiotic interaction, the composition, physiological relevance, the external solid-phase iron and manganese minerals that can and electron transport mechanisms of bacterial nanowires remain serve as terminal electron acceptors for respiration. -
Effects of the Anaerobic Respiration of Shewanella Oneidensis MR-1 on the Stability of Extracellular U(VI) Nanofibers
Microbes Environ. Vol. 28, No. 3, 312–315, 2013 https://www.jstage.jst.go.jp/browse/jsme2 doi:10.1264/jsme2.ME12149 Effects of the Anaerobic Respiration of Shewanella oneidensis MR-1 on the Stability of Extracellular U(VI) Nanofibers SHENGHUA JIANG1,2, and HOR-GIL HUR1* 1School of Environmental Science and Engineering, Gwangju Institute of Science and Technology, Gwangju 500–712, Republic of Korea; and 2International Environmental Analysis and Education Center, Gwangju Institute of Science and Technology, Gwangju 500–712, Republic of Korea (Received July 23, 2012—Accepted April 8, 2013—Published online May 29, 2013) Uranium (VI) is considered to be one of the most widely dispersed and problematic environmental contaminants, due in large part to its high solubility and great mobility in natural aquatic systems. We previously reported that under anaerobic conditions, Shewanella oneidensis MR-1 grown in medium containing uranyl acetate rapidly accumulated 3 long, extracellular, ultrafine U(VI) nanofibers composed of polycrystalline chains of discrete meta-schoepite (UO ·2H2O) nanocrystallites. Wild-type MR-1 finally transformed the uranium (VI) nanofibers to uranium (IV) nanoparticles via further reduction. In order to investigate the influence of the respiratory chain in the uranium transformation process, a series of mutant strains lacking a periplasmic cytochrome MtrA, outer membrane (OM) cytochrome MtrC and OmcA, a tetraheme cytochrome CymA anchored to the cytoplasmic membrane, and a trans-OM protein MtrB, were tested in this study. Although all the mutants produced U(VI) nanofibers like the wild type, the transformation rates from U(VI) nanofibers to U(IV) nanoparticles varied; in particular, the mutant with deletion in tetraheme cytochrome CymA stably maintained the uranium (VI) nanofibers, suggesting that the respiratory chain of S. -
CUED Phd and Mphil Thesis Classes
High-throughput Experimental and Computational Studies of Bacterial Evolution Lars Barquist Queens' College University of Cambridge A thesis submitted for the degree of Doctor of Philosophy 23 August 2013 Arrakis teaches the attitude of the knife { chopping off what's incomplete and saying: \Now it's complete because it's ended here." Collected Sayings of Muad'dib Declaration High-throughput Experimental and Computational Studies of Bacterial Evolution The work presented in this dissertation was carried out at the Wellcome Trust Sanger Institute between October 2009 and August 2013. This dissertation is the result of my own work and includes nothing which is the outcome of work done in collaboration except where specifically indicated in the text. This dissertation does not exceed the limit of 60,000 words as specified by the Faculty of Biology Degree Committee. This dissertation has been typeset in 12pt Computer Modern font using LATEX according to the specifications set by the Board of Graduate Studies and the Faculty of Biology Degree Committee. No part of this dissertation or anything substantially similar has been or is being submitted for any other qualification at any other university. Acknowledgements I have been tremendously fortunate to spend the past four years on the Wellcome Trust Genome Campus at the Sanger Institute and the European Bioinformatics Institute. I would like to thank foremost my main collaborators on the studies described in this thesis: Paul Gardner and Gemma Langridge. Their contributions and support have been invaluable. I would also like to thank my supervisor, Alex Bateman, for giving me the freedom to pursue a wide range of projects during my time in his group and for advice. -
A Biofilm Enhanced Miniature Microbial Fuel Cell Using Shewanella Oneidensis DSP10 and Oxygen Reduction Cathodes
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by UNL | Libraries University of Nebraska - Lincoln DigitalCommons@University of Nebraska - Lincoln U.S. Navy Research U.S. Department of Defense 2007 A biofilm enhanced miniature microbial fuel cell using Shewanella oneidensis DSP10 and oxygen reduction cathodes Justin C. Biffinger US Naval Research Laboratory, [email protected] Jeremy Pietron Naval Research Laboratory Ricky Ray Naval Research Laboratory Brenda J. Little Naval Research Laboratory, [email protected] Bradley R. Ringeisen Naval Research Laboratory, [email protected] Follow this and additional works at: https://digitalcommons.unl.edu/usnavyresearch Part of the Operations Research, Systems Engineering and Industrial Engineering Commons Biffinger, Justin C.; Pietron, Jeremy; Ray, Ricky; Little, Brenda J.; and Ringeisen, Bradley R., "A biofilm enhanced miniature microbial fuel cell using Shewanella oneidensis DSP10 and oxygen reduction cathodes" (2007). U.S. Navy Research. 14. https://digitalcommons.unl.edu/usnavyresearch/14 This Article is brought to you for free and open access by the U.S. Department of Defense at DigitalCommons@University of Nebraska - Lincoln. It has been accepted for inclusion in U.S. Navy Research by an authorized administrator of DigitalCommons@University of Nebraska - Lincoln. Biosensors and Bioelectronics 22 (2007) 1672–1679 A biofilm enhanced miniature microbial fuel cell using Shewanella oneidensis DSP10 and oxygen reduction cathodes Justin C. Biffinger a, Jeremy Pietron a, Ricky Ray b, Brenda Little b, Bradley R. Ringeisen a,∗ a Chemistry Division, Naval Research Laboratory, 4555 Overlook Avenue, SW, Washington, DC 20375, United States b Oceanography Division, Naval Research Laboratory, Building 1009, John C. -
Growth Protein Involved in Aerobic and Anoxic Shewanella Oneidensis
Shewanella oneidensis MR-1 Sensory Box Protein Involved in Aerobic and Anoxic Growth A. Sundararajan, J. Kurowski, T. Yan, D. M. Klingeman, M. Downloaded from P. Joachimiak, J. Zhou, B. Naranjo, J. A. Gralnick and M. W. Fields Appl. Environ. Microbiol. 2011, 77(13):4647. DOI: 10.1128/AEM.03003-10. Published Ahead of Print 20 May 2011. http://aem.asm.org/ Updated information and services can be found at: http://aem.asm.org/content/77/13/4647 These include: REFERENCES This article cites 60 articles, 33 of which can be accessed free at: http://aem.asm.org/content/77/13/4647#ref-list-1 on April 26, 2012 by MONTANA STATE UNIV AT BOZEMAN CONTENT ALERTS Receive: RSS Feeds, eTOCs, free email alerts (when new articles cite this article), more» Information about commercial reprint orders: http://aem.asm.org/site/misc/reprints.xhtml To subscribe to to another ASM Journal go to: http://journals.asm.org/site/subscriptions/ APPLIED AND ENVIRONMENTAL MICROBIOLOGY, July 2011, p. 4647–4656 Vol. 77, No. 13 0099-2240/11/$12.00 doi:10.1128/AEM.03003-10 Copyright © 2011, American Society for Microbiology. All Rights Reserved. Shewanella oneidensis MR-1 Sensory Box Protein Involved in Aerobic and Anoxic Growth A. Sundararajan,1,2,3 J. Kurowski,1 T. Yan,4 D. M. Klingeman,4 M. P. Joachimiak,5,8 J. Zhou,6,8 B. Naranjo,7 J. A. Gralnick,7 and M. W. Fields2,3,8* Downloaded from Department of Microbiology, Miami University, Oxford, Ohio 450561; Department of Microbiology, Montana State University, Bozeman, Montana2; Center for Biofilm Engineering, Montana State University, Bozeman, Montana3; Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee4; Lawrence Berkeley National Laboratory, Berkeley, California5; Institute for Environmental Genomics, University of Oklahoma, Norman, Oklahoma6; Department of Microbiology and BioTechnology Institute, University of Minnesota, St. -
Genomic Signatures of Sex, Selection and Speciation in the Microbial World
Genomic signatures of sex, selection and speciation in the microbial world by B. Jesse Shapiro B.Sc. Biology McGill University, 2003 M.Sc. Integrative Bioscience Oxford University, 2004 SUBMITTED TO THE PROGRAM IN COMPUTATIONAL AND SYSTEMS BIOLOGY IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY IN COMPUTATIONAL AND SYSTEMS BIOLOGY AT THE MASSACHUSETTS INSTITUTE OF TECHNOLOGY SEPTEMBER 2010 © 2010 Massachusetts Institute of Technology All rights reserved Signature of Author:...……………………………………………………………………………………….. Program in Computational and Systems Biology August 6, 2010 Certified by:…………………………………………………………………………………………………. Eric J. Alm Assistant Professor of Civil and Environmental Engineering and Biological Engineering Thesis Supervisor Accepted by:………………………………………………………………………………………………… Christopher B. Burge Professor of Biology and Biological Engineering Chair, Computational and Systems Biology Ph.D. Graduate Committee 1 Genomic signatures of sex, selection and speciation in the microbial world by B. Jesse Shapiro Submitted to the Program in Computational and Systems Biology on August 6, 2010 in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy in Computational and Systems Biology ABSTRACT Understanding the microbial world is key to understanding global biogeochemistry, human health and disease, yet this world is largely inaccessible. Microbial genomes, an increasingly accessible data source, provide an ideal entry point. The genome sequences of different microbes may be compared using the tools of population genetics to infer important genetic changes allowing them to diversify ecologically and adapt to distinct ecological niches. Yet the toolkit of population genetics was developed largely with sexual eukaryotes in mind. In this work, I assess and develop tools for inferring natural selection in microbial genomes. -
Shewanella Oneidensis Arca Mutation Impairs Aerobic Growth Mainly by Compromising Translation
life Article Shewanella oneidensis arcA Mutation Impairs Aerobic Growth Mainly by Compromising Translation Peilu Xie, Jiahao Wang, Huihui Liang and Haichun Gao * Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou 310058, China; [email protected] (P.X.); [email protected] (J.W.); [email protected] (H.L.) * Correspondence: author: [email protected] Abstract: Arc (anoxic redox control), one of the most intensely investigated two-component regu- latory systems in γ-proteobacteria, plays a major role in mediating the metabolic transition from aerobiosis to anaerobiosis. In Shewanella oneidensis, a research model for respiratory versatility, Arc is crucial for aerobic growth. However, how this occurs remains largely unknown. In this study, we demonstrated that the loss of the response regulator ArcA distorts the correlation between tran- scription and translation by inhibiting the ribosome biosynthesis. This effect largely underlies the growth defect because it concurs with the effect of chloramphenicol, which impairs translation. Reduced transcription of ArcA-dependent ribosomal protein S1 appears to have a significant impact on ribosome assembly. We further show that the lowered translation efficiency is not accountable for the envelope defect, another major defect resulting from the ArcA loss. Overall, our results suggest that although the arcA mutation impairs growth through multi-fold complex impacts in physiology, the reduced translation efficacy appears to be a major cause for the phenotype, demonstrating that Arc is a primary system that coordinates proteomic resources with metabolism in S. oneidensis. Citation: Xie, P.; Wang, J.; Liang, H.; Keywords: ArcA; regulation; aerobic growth; translation efficacy; peptide transportation Gao, H. -
Electrically Conductive Bacterial Nanowires Produced by Shewanella Oneidensis Strain MR-1 and Other Microorganisms
Electrically conductive bacterial nanowires produced by Shewanella oneidensis strain MR-1 and other microorganisms Yuri A. Gorby*†, Svetlana Yanina*, Jeffrey S. McLean*, Kevin M. Rosso*, Dianne Moyles‡, Alice Dohnalkova*, Terry J. Beveridge‡, In Seop Chang§, Byung Hong Kim¶, Kyung Shik Kim¶, David E. Culley*, Samantha B. Reed*, Margaret F. Romine*, Daad A. Saffariniʈ, Eric A. Hill*, Liang Shi*, Dwayne A. Elias*,**, David W. Kennedy*, Grigoriy Pinchuk*, Kazuya Watanabe††, Shun’ichi Ishii††, Bruce Logan‡‡, Kenneth H. Nealson§§, and Jim K. Fredrickson* *Pacific Northwest National Laboratory, Richland, WA 99352; ‡Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, Canada N1G 2W1; ¶Water Environment and Remediation Research Center, Korea Institute of Science and Technology, Seoul 136-791, Korea; §Department of Environmental Science and Engineering, Gwangju Institute of Science and Technology, Gwangju 500-712, Korea; ʈDepartment of Biological Sciences, University of Wisconsin, Milwaukee, WI 53211; **Department of Agriculture Biochemistry, University of Missouri, Columbia, MO 65211; ††Marine Biotechnology Institute, Heita, Kamaishi, Iwate 026-0001, Japan; ‡‡Department of Environmental Engineering, Pennsylvania State University, University Park, PA 16802; and §§Department of Earth Sciences, University of Southern California, Los Angeles, CA 90089 Communicated by J. Woodland Hastings, Harvard University, Cambridge, MA, June 6, 2006 (received for review September 20, 2005) Shewanella oneidensis MR-1 produced electrically conductive pi- from 50 to Ͼ150 nm in diameter and extending tens of microns or lus-like appendages called bacterial nanowires in direct response longer (Fig. 1A; and see Fig. 5A, which is published as supporting to electron-acceptor limitation. Mutants deficient in genes for information on the PNAS web site). -
Respiration in Shewanella Oneidensis
Physiological Roles of ArcA, Crp, and EtrA and Their Interactive Control on Aerobic and Anaerobic Respiration in Shewanella oneidensis Haichun Gao1,2*, Xiaohu Wang3¤, Zamin K. Yang4, Jingrong Chen2, Yili Liang2, Haijiang Chen1, Timothy Palzkill3, Jizhong Zhou2* 1 Institute of Microbiology and College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China, 2 Institute for Environmental Genomics and Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma, United States of America, 3 Department of Pharmacology & Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, United States of America, 4 Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America Abstract In the genome of Shewanella oneidensis, genes encoding the global regulators ArcA, Crp, and EtrA have been identified. All these proteins deviate from their counterparts in E. coli significantly in terms of functionality and regulon. It is worth investigating the involvement and relationship of these global regulators in aerobic and anaerobic respiration in S. oneidensis. In this study, the impact of the transcriptional factors ArcA, Crp, and EtrA on aerobic and anaerobic respiration in S. oneidensis were assessed. While all these proteins appeared to be functional in vivo, the importance of individual proteins in these two major biological processes differed. The ArcA transcriptional factor was critical in aerobic respiration while the Crp protein was indispensible in anaerobic respiration. Using a newly developed reporter system, it was found that expression of arcA and etrA was not influenced by growth conditions but transcription of crp was induced by removal of oxygen. An analysis of the impact of each protein on transcription of the others revealed that Crp expression was independent of the other factors whereas ArcA repressed both etrA and its own transcription while EtrA also repressed arcA transcription. -
Life in the Cold Biosphere: the Ecology of Psychrophile
Life in the cold biosphere: The ecology of psychrophile communities, genomes, and genes Jeff Shovlowsky Bowman A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy University of Washington 2014 Reading Committee: Jody W. Deming, Chair John A. Baross Virginia E. Armbrust Program Authorized to Offer Degree: School of Oceanography i © Copyright 2014 Jeff Shovlowsky Bowman ii Statement of Work This thesis includes previously published and submitted work (Chapters 2−4, Appendix 1). The concept for Chapter 3 and Appendix 1 came from a proposal by JWD to NSF PLR (0908724). The remaining chapters and appendices were conceived and designed by JSB. JSB performed the analysis and writing for all chapters with guidance and editing from JWD and co- authors as listed in the citation for each chapter (see individual chapters). iii Acknowledgements First and foremost I would like to thank Jody Deming for her patience and guidance through the many ups and downs of this dissertation, and all the opportunities for fieldwork and collaboration. The members of my committee, Drs. John Baross, Ginger Armbrust, Bob Morris, Seelye Martin, Julian Sachs, and Dale Winebrenner provided valuable additional guidance. The fieldwork described in Chapters 2, 3, and 4, and Appendices 1 and 2 would not have been possible without the help of dedicated guides and support staff. In particular I would like to thank Nok Asker and Lewis Brower for giving me a sample of their vast knowledge of sea ice and the polar environment, and the crew of the icebreaker Oden for a safe and fascinating voyage to the North Pole. -
Transcriptome Analysis Reveals a Stress Response of Shewanella Oneidensis Deprived of Background Levels of Ionizing Radiation
RESEARCH ARTICLE Transcriptome analysis reveals a stress response of Shewanella oneidensis deprived of background levels of ionizing radiation Hugo Castillo1*, Xiaoping Li2, Faye Schilkey3, Geoffrey B. Smith1 1 Department of Biology, New Mexico State University, Las Cruces, NM, United States of America, 2 Department of Botany and Plant Pathology, Oregon State University, Hermiston, OR, United States of America, 3 National Center for Genome Resources, Santa Fe, NM, United States of America * [email protected] a1111111111 a1111111111 a1111111111 a1111111111 Abstract a1111111111 Natural ionizing background radiation has exerted a constant pressure on organisms since the first forms of life appeared on Earth, so that cells have developed molecular mecha- nisms to avoid or repair damages caused directly by radiation or indirectly by radiation- induced reactive oxygen species (ROS). In the present study, we investigated the transcrip- OPEN ACCESS tional effect of depriving Shewanella oneidensis cultures of background levels of radiation Citation: Castillo H, Li X, Schilkey F, Smith GB by growing the cells in a mine 655 m underground, thus reducing the dose rate from 72.1 to (2018) Transcriptome analysis reveals a stress 0.9 nGy h-1 from control to treatment, respectively. RNASeq transcriptome analysis showed response of Shewanella oneidensis deprived of background levels of ionizing radiation. PLoS ONE the differential expression of 4.6 and 7.6% of the S. oneidensis genome during early- and 13(5): e0196472. https://doi.org/10.1371/journal. late-exponential phases of growth, respectively. The greatest change observed in the treat- pone.0196472 ment was the downregulation of ribosomal proteins (21% of all annotated ribosomal protein Editor: Y-h. -
Shewanella Oneidensis
RESEARCH ARTICLE Genome sequence of the dissimilatory metal ion–reducing bacterium Shewanella oneidensis John F.Heidelberg1,2, Ian T. Paulsen1,3, Karen E. Nelson1, Eric J. Gaidos4,5, William C. Nelson1, Timothy D. Read1, Jonathan A. Eisen1,3, Rekha Seshadri1, Naomi Ward1,2, Barbara Methe1, Rebecca A. Clayton1, Terry Meyer6, Alexandre Tsapin4, James Scott7, Maureen Beanan1, Lauren Brinkac1, Sean Daugherty1, Robert T. DeBoy1, Robert J. Dodson1, A. Scott Durkin1, Daniel H. Haft1, James F.Kolonay1, Ramana Madupu1, Jeremy D. Peterson1, Lowell A. Umayam1, Owen White1, Alex M. Wolf1, Jessica Vamathevan1, Janice Weidman1, Marjorie Impraim1, Kathy Lee1, Kristy Berry1, Chris Lee1, Jacob Mueller1, Hoda Khouri1, John Gill1, Terry R. Utterback1, Lisa A. McDonald1, Tamara V. Feldblyum1, Hamilton O. Smith1,8, J. Craig Venter1,9, Kenneth H. Nealson4,10, and Claire M. Fraser1,11* Published online 7 October 2002; doi:10.1038/nbt749 Shewanella oneidensis is an important model organism for bioremediation studies because of its diverse res- piratory capabilities, conferred in part by multicomponent, branched electron transport systems. Here we report the sequencing of the S. oneidensis genome, which consists of a 4,969,803–base pair circular chromo- http://www.nature.com/naturebiotechnology some with 4,758 predicted protein-encoding open reading frames (CDS) and a 161,613–base pair plasmid with 173 CDSs. We identified the first Shewanella lambda-like phage, providing a potential tool for further genome engineering. Genome analysis revealed 39 c-type cytochromes, including 32 previously unidentified in S. oneidensis, and a novel periplasmic [Fe] hydrogenase, which are integral members of the electron trans- port system.