A Novel Optical Microscope for Imaging Large Embryos And
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A Novel Optical Microscope for Imaging Large
RESEARCH ARTICLE A novel optical microscope for imaging large embryos and tissue volumes with sub-cellular resolution throughout Gail McConnell1*, Johanna Tra¨ ga˚ rdh1, Rumelo Amor1, John Dempster1, Es Reid1, William Bradshaw Amos1,2 1Centre for Biophotonics, Strathclyde Institute for Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United Kingdom; 2MRC Laboratory of Molecular Biology, Cambridge, United Kingdom Abstract Current optical microscope objectives of low magnification have low numerical aperture and therefore have too little depth resolution and discrimination to perform well in confocal and nonlinear microscopy. This is a serious limitation in important areas, including the phenotypic screening of human genes in transgenic mice by study of embryos undergoing advanced organogenesis. We have built an optical lens system for 3D imaging of objects up to 6 mm wide and 3 mm thick with depth resolution of only a few microns instead of the tens of microns currently attained, allowing sub-cellular detail to be resolved throughout the volume. We present this lens, called the Mesolens, with performance data and images from biological specimens including confocal images of whole fixed and intact fluorescently-stained 12.5-day old mouse embryos. DOI: 10.7554/eLife.18659.001 Introduction During experiments with laser scanning confocal microscopes in the mid-1980s, it became obvious that the optical sectioning, which is the main advantage of the confocal method, did not work with *For correspondence: the available low-magnification objectives, because of their low numerical aperture (N.A.) [email protected] (White et al., 1987). In specimens such as mouse embryos at the 10–12.5 day stage, when the major Competing interest: See organs are developing (Kaufman, 1992), it was impossible to see individual cells in the interior page 14 despite the lateral (XY) resolution being sufficient. -
Monday, September 14
Monday, September 14 8:00 AM Registration Open 8:45 AM Welcome Remarks Tom Baer, SPRC Integration Technologies for Dense, Low-Power Optical Transceivers Jim Harris, Session Chair Narrow linewidth widely tunable lasers using American 9:00 AM John Bowers, UCSB Institute of Manufacturing (AIM) of Photonics Embracing diversity - MEMS-based hybrid optical 9:30 AM Bardia Pezeshki, Kaiam Inc. packaging that brings out the best of each technology A Decade of Data Center Network Architecture and 10:00 AM Amin Vahdat, Google Implementation at Google 10:30 AM BREAK Communications & Networking for Data Centers Joe Kahn, Session Chair 11:00 AM Optical Technology Requirements Hong Liu, Google 11:30 AM Hardware Defined Networking within Datacenters George Papen, UCSD 12:00 PM 100G Single-Laser Links for Data Centers Jose Krause Perin, Stanford 12:30 PM Poster Intros 1:00 PM LUNCH Fiber Sensors Michel Digonnet, Session Chair Luc Thevenaz, Ecole 2:00 PM Distributed fibre sensing : state-of-the-art and perspectives Polytechnique Federale Lausanne 2:30 PM Measuring attostrains with a slow-light fiber Bragg grating George Skolianos, Stanford Utilizing Fiber Sensor Technology to Miniaturize the Antonio Gellineau, KLA 2:45 PM Atomic Force Microscope Tencor Identification of heart muscle cells using an ultrasensitive 3:15 PM Cathy Jan, Stanford fiber hydrophone 3:30 PM BREAK Unconventional Applications of Guided Light Olav Solgaard, Session Chair Fetah Benabid, XLIM 4:00 PM In-fiber atom optics using Kagome hollow fiber Research Institute Time differentiation deformability cytometry using 4:30 PM Saara Khan, Stanford evanescent fields of waveguides for single cell analysis Ultra-Thin Rigid or Flexible Endoscope using a Multi- 4:45 PM Ruo Yu Gu, Stanford Mode Fiber Caroline Boudoux, Ecole 5:15 PM Double-clad fiber couplers for multimodal sensing Polytechnique Montreal Reception & Dinner 5:30 PM Stanford Faculty Club After-dinner presentation by Prof. -
New Chief Executive As MRC Gets Cash Boost
AUTUMN 2007 News from the Medical Research Council New Chief Executive as MRC gets cash boost A new Chief Executive, Sir Leszek Borysiewicz, took the helm of the MRC at the beginning of October. Sir Leszek, who comes from Imperial College London where he was Deputy Rector, spoke of his excitement about leading the MRC at a time of change and opportunity for the organisation: “I’m thrilled by the chance to work across the whole spectrum of biomedical science and to help to make a difference in relation to healthcare for individuals in the UK and globally.” The chairman of the MRC, Sir John Chisholm, welcomed Sir Leszek: “He is the perfect person to lead the MRC in the new environment of coordinated health research in the UK. I am delighted he’ll join us – his stature as a scientist and clinician reflects the importance of the role the MRC will play in a coordinated strategy for turning research findings into healthcare.” Timely arrival Sir Leszek joined the MRC just days before the Chancellor announced a significant increase in the organisation’s budget, from £543 million to £682 million a year by 2010. In his pre-budget report to the House of Commons on 10 October, Alistair Darling explained that he was funding recognised across the world. And so more British medical discovery can in full the recommendations of Sir David Cooksey’s review of publicly be translated into new health drugs, treatments and preventions… We funded health research including a single strategy for health research in will expand the single fund for health research to £1.7 billion by 2010.” the UK overseen by an Office for the Strategic Coordination of Health Research (OSCHR). -
Funding the Trust Offers 12 Director’S Report 13 Distribution of Funds in 2014 14 2014 in Numbers 15 Summarised Financial Information
ANNUAL REVIEW 2014 2 3 CONTENTS Page 04 Introduction 06 Chairman’s foreword 08 History of the Leverhulme Trust 11 Funding the Trust offers 12 Director’s report 13 Distribution of funds in 2014 14 2014 in numbers 15 Summarised financial information Page 16 Awards in Focus 18 Losing time and temper: how people learned to live with railway time 20 Unlocking friction: unifying the nanoscale and mesoscale 22 Highland encounters: practice, perception and power in the mountains of the ancient Middle East 24 Kinship, morality and the emotions: the evolution of human social psychology 25 Sidonius Apollinaris: a comprehensive commentary for the twenty-first century 26 Promoting national and imperial identities: museums in Austria-Hungary 28 Alice in space: contexts for Lewis Carroll 30 DNA: the knotted molecule of life 31 Seabirds as bio-indicators: relating breeding strategy to the marine environment 32 At home in the Himalayas: rethinking photography in the hill stations of British India 34 Making a mark: imagery and process in the British and Irish Neolithic 35 Assembling the ancient islands of Japan 36 Pierre Soulages: Radical Abstraction 38 Early Chinese sculpture in the Asian context – art history and technology 40 ‘A Graphic War’: design at home and on the front lines (1914–1918) 42 Cultural propaganda agencies in colonial Cyprus and their policies 44 Act big, get big: bone cell activity scaling among species as a skeletal adaptation mechanism 46 Black Sea sketches: music, place and people 48 Why chytrids are leaving amphibians ‘naked’ 50 ‘The Walrus Ivory Owl’ Page 52 What Happened Next 54 Dr Chris Jiggins 56 Dr Hannes Baumann 58 Dr Lucie Green 60 Professor Giorgio Riello 62 Professor Kim Bard 64 Professor Martin Hairer 65 Dr Robert Nudds 66 Professor Rana Mitter Page 68 Awards Made 5 INTRODUCTION The Leverhulme Trust was established by the Will of William Hesketh Lever, one of the great entrepreneurs and philanthropists of the Victorian age. -
The Royal Society of Edinburgh Review 2005 (Session 2003-2004)
The Royal Society of Edinburgh Review 2005 (Session 2003-2004) The Royal Society of Edinburgh Review 2005 Printed in Great Britain by MacKay & Inglis Limited, Glasgow, G42 0PQ ISSN 1476-4334 THE ROYAL SOCIETY OF EDINBURGH REVIEW OF THE SESSION 2003-2004 The Royal Society of Edinburgh 22-26 George Street Edinburgh, EH2 2PQ Telephone : 0131 240 5000 Fax : 0131 240 5024 email : [email protected] Scottish Charity No SC000470 Printed in Great Britain by MacKay and Inglis Ltd, Glasgow G42 0PQ Cover illustration by Aird McKinstrie. Design by Jennifer Cameron THE ROYAL SOCIETY OF EDINBURGH REVIEW OF THE SESSION 2003-2004 PUBLISHED BY THE RSE SCOTLAND FOUNDATION ISSN 1476-4342 CONTENTS Proceedings of the Ordinary Meetings .................................... 3 Proceedings of the Statutory General Meeting ....................... 5 Trustees Report to 31 March 2004 ....................................... 19 Auditor’s Report and Accounts.............................................. 35 Schedule of Investments ....................................................... 55 Activities Prize Lectures ..................................................................... 59 Lectures............................................................................ 109 Conferences, Symposia, Workshops, Seminars and Discussion Forums ............................................................ 159 Publications ...................................................................... 195 The Scottish Science Advisory Committee ........................ 197 Evidence, -
Schniete, Jan and Franssen, Aimee and Dempster, John And
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by University of Strathclyde Institutional Repository Schniete, Jan and Franssen, Aimee and Dempster, John and Bushell, Trevor J and Amos, William Bradshaw and McConnell, Gail (2018) Fast optical sectioning for widefield fluorescence mesoscopy with the mesolens based on HiLo microscopy. Scientific Reports, 8. ISSN 2045- 2322 , http://dx.doi.org/10.1038/s41598-018-34516-2 This version is available at https://strathprints.strath.ac.uk/65850/ Strathprints is designed to allow users to access the research output of the University of Strathclyde. Unless otherwise explicitly stated on the manuscript, Copyright © and Moral Rights for the papers on this site are retained by the individual authors and/or other copyright owners. Please check the manuscript for details of any other licences that may have been applied. You may not engage in further distribution of the material for any profitmaking activities or any commercial gain. You may freely distribute both the url (https://strathprints.strath.ac.uk/) and the content of this paper for research or private study, educational, or not-for-profit purposes without prior permission or charge. Any correspondence concerning this service should be sent to the Strathprints administrator: [email protected] The Strathprints institutional repository (https://strathprints.strath.ac.uk) is a digital archive of University of Strathclyde research outputs. It has been developed to disseminate open access research outputs, -
University of Strathclyde Calendar 2008-09 Part 1 Staff Lists And
University of Strathclyde Calendar 2008-09 Part 1 Staff Lists and General Regulations ISBN 1 85098 590 2 ISSN 0305-3180 © University of Strathclyde 2008 The University of Strathclyde is a registered trademark Printed by Bell & Bain Ltd, Glasgow The Calendar is published in four parts: Part 1 contains the University Charter, Statutes and Ordinances, together with staff lists, Regulations 1-7 and an Appendix (History of the University, Armorial Bearings, University Chairs and Honorary Graduates). Part 2 contains Regulations 15-17 covering the course regulations for undergraduate and integrated master’s degrees of the five Faculties and elective classes. Part 3 contains Regulations 19-30 covering the postgraduate, continuing education, sub-degree courses and prize regulations of the five Faculties. This edition of the Calendar is as far as possible up to date and accurate at 15 August 2008. Changes and restrictions are made from time to time and the University reserves the right to add, amend or withdraw courses and facilities, to restrict student numbers and to make any other alterations, as it may deem necessary and desirable. Changes are published by incorporation in the next edition of the University Calendar. Any queries about the contents of the University Calendar should be directed to the Editor of the University Calendar, Secretariat, University of Strathclyde, Glasgow G1 1XQ (Telephone 0141 548 4967). Official Publications Calendar The University of Strathclyde Calendar is published annually in September, price £15 exclusive of packing. Copies are available from the Editor of the University Calendar, University of Strathclyde, 16 Richmond Street, Glasgow G1 1XQ (Telephone 0141 548 4967). -
University of Strathclyde Calendar 2007-08 Part 1 Staff Lists and General Regulations
University of Strathclyde Calendar 2007-08 Part 1 Staff Lists and General Regulations ISBN 1 85098 590 2 ISSN 0305-3180 © University of Strathclyde 2007 The University of Strathclyde is a registered trademark Printed by Bell and Bain Ltd, Glasgow The Calendar is published in four parts: Part 1 contains the University Charter, Statutes and Ordinances, together with staff lists, Regulations 1-7 and an Appendix (History of the University, Armorial Bearings, University Chairs and Honorary Graduates). Part 2 contains Regulations 15-17 covering the course regulations for undergraduate and integrated masters degrees of the five Faculties and elective classes for students registered in the first year with effect from session 2003-04. Part 3 contains Regulations 19-30 covering the postgraduate, continuing education, sub-degree courses and prize regulations of the five Faculties. This edition of the Calendar is as far as possible up to date and accurate at 16 August 2007. Changes and restrictions are made from time to time and the University reserves the right to add, amend or withdraw courses and facilities, to restrict student numbers and to make any other alterations, as it may deem necessary and desirable. Changes are published by incorporation in the next edition of the University Calendar. Any queries about the contents of the University Calendar should be directed to the Editor of the University Calendar, Secretariat, University of Strathclyde, Glasgow G1 1XQ (Telephone 0141 548 4967). Official Publications Calendar The University of Strathclyde Calendar is published annually in September, price £15 exclusive of packing. Copies are available from the Editor of the University Calendar, University of Strathclyde, 16 Richmond Street, Glasgow G1 1XQ (Telephone 0141 548 4967). -
Awards Made 2014
Awards made 2014 LEVERHULME DOCTORAL University of Sheffield Dr Christopher Bayliss SCHOLARSHIPS Professor Rob Freckleton University of Leicester Institutions received £1,050,000 to fund Centre for advanced biological modelling Mutate and survive: how bacteria fight viruses fifteen PhD students over three years. (CABM) £158,130 Durham University University of Southampton Dr Elizabeth Bayne Professor Ludmilla Jordanova Professor Damon Teagle University of Edinburgh Leverhulme Durham doctoral training Understanding maritime futures: Regulation of RNAi-directed chromatin programme in visual culture opportunities, challenges, and threats modification by ubiquitin and SUMO £175,891 University of Edinburgh University of Warwick Professor Jan Palmowski Dr Morgan Beeby Professor James Smith Bridges: bringing together the social and Imperial College London Perfect storm – interdisciplinary doctoral mathematical sciences to understand social Evolutionary pathways to rotary motors: training information evolution and mechanism of the archaellum £117,620 University of Glasgow RESEARCH PROGRAMME GRANTS Professor Karen Lury Professor Simon Belt Collections: an Enlightenment pedagogy for The nature of knots University of Plymouth the twenty-first century Quantification of sea ice carbon within Arctic Dr Dorothy Buck ecosystems University of Huddersfield Imperial College London £173,049 Professor Martin Richards Knots in nature: DNA, the knotted molecule Genetic journeys into history: the next of life Dr Alexander Belton generation £1,739,476 University -
Download Her Thesis Here
INVESTIGATION OF THE OPTICAL PROPERTIES, COMPOSITION AND LOCAL STRUCTURE OF InGaN A Thesis Submitted to The Department of Physics and Applied Physics University of Strathclyde For the Degree of Doctor of Philosophy By Madeleine Eve White April 2002 DECLARATION OF AUTHOR’S RIGHTS The copyright of this thesis belongs to the author under the terms of the United Kingdom Copyright Acts as qualified by University of Strathclyde Regulation 3.49. Due acknowledgement must always be made of the use of any material contained in, or derived from, this thesis. ABSTRACT This thesis presents work on the optical properties, composition and local structure of InGaN. Low temperature photoluminescence (PL) spectroscopy of InGaN epilayers was used to study linewidth, intensity and uniformity of the samples. Temperature dependence of the PL spectrum showed a decrease in the peak emission energy with increasing temperature for one epilayer sample and an ‘S’- shape temperature dependence of peak emission energy for a second sample. Composition analysis of the epilayers using three different experimental techniques; Rutherford backscattering spectrometry (RBS), electron probe microanalysis (EPMA) and extended X-ray absorption fine structure (EXAFS), found that the indium nitride fraction varies linearly with the PL peak emission energy. Confocal microscopy of InGaN epilayer, quantum well (QW) and quantum box (QB) samples revealed that InGaN luminescence segregates into bright and dark spots, ~1m in diameter, and indicates that the origin of InGaN luminescence is of sub m-scale. InGaN local structure parameters were derived from EXAFS measurements. Epilayer samples exhibited a single-phase InGaN alloy while a QB sample showed a two-phase mixture of components, one of which is nearly pure InN.