Monday, September 14
Total Page:16
File Type:pdf, Size:1020Kb
Load more
Recommended publications
-
A Novel Optical Microscope for Imaging Large
RESEARCH ARTICLE A novel optical microscope for imaging large embryos and tissue volumes with sub-cellular resolution throughout Gail McConnell1*, Johanna Tra¨ ga˚ rdh1, Rumelo Amor1, John Dempster1, Es Reid1, William Bradshaw Amos1,2 1Centre for Biophotonics, Strathclyde Institute for Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United Kingdom; 2MRC Laboratory of Molecular Biology, Cambridge, United Kingdom Abstract Current optical microscope objectives of low magnification have low numerical aperture and therefore have too little depth resolution and discrimination to perform well in confocal and nonlinear microscopy. This is a serious limitation in important areas, including the phenotypic screening of human genes in transgenic mice by study of embryos undergoing advanced organogenesis. We have built an optical lens system for 3D imaging of objects up to 6 mm wide and 3 mm thick with depth resolution of only a few microns instead of the tens of microns currently attained, allowing sub-cellular detail to be resolved throughout the volume. We present this lens, called the Mesolens, with performance data and images from biological specimens including confocal images of whole fixed and intact fluorescently-stained 12.5-day old mouse embryos. DOI: 10.7554/eLife.18659.001 Introduction During experiments with laser scanning confocal microscopes in the mid-1980s, it became obvious that the optical sectioning, which is the main advantage of the confocal method, did not work with *For correspondence: the available low-magnification objectives, because of their low numerical aperture (N.A.) [email protected] (White et al., 1987). In specimens such as mouse embryos at the 10–12.5 day stage, when the major Competing interest: See organs are developing (Kaufman, 1992), it was impossible to see individual cells in the interior page 14 despite the lateral (XY) resolution being sufficient. -
A Novel Optical Microscope for Imaging Large Embryos And
RESEARCH ARTICLE A novel optical microscope for imaging large embryos and tissue volumes with sub-cellular resolution throughout Gail McConnell1*, Johanna Tra¨ ga˚ rdh1, Rumelo Amor1, John Dempster1, Es Reid1, William Bradshaw Amos1,2 1Centre for Biophotonics, Strathclyde Institute for Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United Kingdom; 2MRC Laboratory of Molecular Biology, Cambridge, United Kingdom Abstract Current optical microscope objectives of low magnification have low numerical aperture and therefore have too little depth resolution and discrimination to perform well in confocal and nonlinear microscopy. This is a serious limitation in important areas, including the phenotypic screening of human genes in transgenic mice by study of embryos undergoing advanced organogenesis. We have built an optical lens system for 3D imaging of objects up to 6 mm wide and 3 mm thick with depth resolution of only a few microns instead of the tens of microns currently attained, allowing sub-cellular detail to be resolved throughout the volume. We present this lens, called the Mesolens, with performance data and images from biological specimens including confocal images of whole fixed and intact fluorescently-stained 12.5-day old mouse embryos. DOI: 10.7554/eLife.18659.001 Introduction During experiments with laser scanning confocal microscopes in the mid-1980s, it became obvious *For correspondence: that the optical sectioning, which is the main advantage of the confocal method, did not work with [email protected] the available low-magnification objectives, because of their low numerical aperture (N.A.) Competing interest: See (White et al., 1987). In specimens such as mouse embryos at the 10–12.5 day stage, when the major page 14 organs are developing (Kaufman, 1992), it was impossible to see individual cells in the interior despite the lateral (XY) resolution being sufficient. -
The Royal Society of Edinburgh Review 2005 (Session 2003-2004)
The Royal Society of Edinburgh Review 2005 (Session 2003-2004) The Royal Society of Edinburgh Review 2005 Printed in Great Britain by MacKay & Inglis Limited, Glasgow, G42 0PQ ISSN 1476-4334 THE ROYAL SOCIETY OF EDINBURGH REVIEW OF THE SESSION 2003-2004 The Royal Society of Edinburgh 22-26 George Street Edinburgh, EH2 2PQ Telephone : 0131 240 5000 Fax : 0131 240 5024 email : [email protected] Scottish Charity No SC000470 Printed in Great Britain by MacKay and Inglis Ltd, Glasgow G42 0PQ Cover illustration by Aird McKinstrie. Design by Jennifer Cameron THE ROYAL SOCIETY OF EDINBURGH REVIEW OF THE SESSION 2003-2004 PUBLISHED BY THE RSE SCOTLAND FOUNDATION ISSN 1476-4342 CONTENTS Proceedings of the Ordinary Meetings .................................... 3 Proceedings of the Statutory General Meeting ....................... 5 Trustees Report to 31 March 2004 ....................................... 19 Auditor’s Report and Accounts.............................................. 35 Schedule of Investments ....................................................... 55 Activities Prize Lectures ..................................................................... 59 Lectures............................................................................ 109 Conferences, Symposia, Workshops, Seminars and Discussion Forums ............................................................ 159 Publications ...................................................................... 195 The Scottish Science Advisory Committee ........................ 197 Evidence, -
Schniete, Jan and Franssen, Aimee and Dempster, John And
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by University of Strathclyde Institutional Repository Schniete, Jan and Franssen, Aimee and Dempster, John and Bushell, Trevor J and Amos, William Bradshaw and McConnell, Gail (2018) Fast optical sectioning for widefield fluorescence mesoscopy with the mesolens based on HiLo microscopy. Scientific Reports, 8. ISSN 2045- 2322 , http://dx.doi.org/10.1038/s41598-018-34516-2 This version is available at https://strathprints.strath.ac.uk/65850/ Strathprints is designed to allow users to access the research output of the University of Strathclyde. Unless otherwise explicitly stated on the manuscript, Copyright © and Moral Rights for the papers on this site are retained by the individual authors and/or other copyright owners. Please check the manuscript for details of any other licences that may have been applied. You may not engage in further distribution of the material for any profitmaking activities or any commercial gain. You may freely distribute both the url (https://strathprints.strath.ac.uk/) and the content of this paper for research or private study, educational, or not-for-profit purposes without prior permission or charge. Any correspondence concerning this service should be sent to the Strathprints administrator: [email protected] The Strathprints institutional repository (https://strathprints.strath.ac.uk) is a digital archive of University of Strathclyde research outputs. It has been developed to disseminate open access research outputs, -
University of Strathclyde Calendar 2008-09 Part 1 Staff Lists And
University of Strathclyde Calendar 2008-09 Part 1 Staff Lists and General Regulations ISBN 1 85098 590 2 ISSN 0305-3180 © University of Strathclyde 2008 The University of Strathclyde is a registered trademark Printed by Bell & Bain Ltd, Glasgow The Calendar is published in four parts: Part 1 contains the University Charter, Statutes and Ordinances, together with staff lists, Regulations 1-7 and an Appendix (History of the University, Armorial Bearings, University Chairs and Honorary Graduates). Part 2 contains Regulations 15-17 covering the course regulations for undergraduate and integrated master’s degrees of the five Faculties and elective classes. Part 3 contains Regulations 19-30 covering the postgraduate, continuing education, sub-degree courses and prize regulations of the five Faculties. This edition of the Calendar is as far as possible up to date and accurate at 15 August 2008. Changes and restrictions are made from time to time and the University reserves the right to add, amend or withdraw courses and facilities, to restrict student numbers and to make any other alterations, as it may deem necessary and desirable. Changes are published by incorporation in the next edition of the University Calendar. Any queries about the contents of the University Calendar should be directed to the Editor of the University Calendar, Secretariat, University of Strathclyde, Glasgow G1 1XQ (Telephone 0141 548 4967). Official Publications Calendar The University of Strathclyde Calendar is published annually in September, price £15 exclusive of packing. Copies are available from the Editor of the University Calendar, University of Strathclyde, 16 Richmond Street, Glasgow G1 1XQ (Telephone 0141 548 4967). -
University of Strathclyde Calendar 2007-08 Part 1 Staff Lists and General Regulations
University of Strathclyde Calendar 2007-08 Part 1 Staff Lists and General Regulations ISBN 1 85098 590 2 ISSN 0305-3180 © University of Strathclyde 2007 The University of Strathclyde is a registered trademark Printed by Bell and Bain Ltd, Glasgow The Calendar is published in four parts: Part 1 contains the University Charter, Statutes and Ordinances, together with staff lists, Regulations 1-7 and an Appendix (History of the University, Armorial Bearings, University Chairs and Honorary Graduates). Part 2 contains Regulations 15-17 covering the course regulations for undergraduate and integrated masters degrees of the five Faculties and elective classes for students registered in the first year with effect from session 2003-04. Part 3 contains Regulations 19-30 covering the postgraduate, continuing education, sub-degree courses and prize regulations of the five Faculties. This edition of the Calendar is as far as possible up to date and accurate at 16 August 2007. Changes and restrictions are made from time to time and the University reserves the right to add, amend or withdraw courses and facilities, to restrict student numbers and to make any other alterations, as it may deem necessary and desirable. Changes are published by incorporation in the next edition of the University Calendar. Any queries about the contents of the University Calendar should be directed to the Editor of the University Calendar, Secretariat, University of Strathclyde, Glasgow G1 1XQ (Telephone 0141 548 4967). Official Publications Calendar The University of Strathclyde Calendar is published annually in September, price £15 exclusive of packing. Copies are available from the Editor of the University Calendar, University of Strathclyde, 16 Richmond Street, Glasgow G1 1XQ (Telephone 0141 548 4967). -
Download Her Thesis Here
INVESTIGATION OF THE OPTICAL PROPERTIES, COMPOSITION AND LOCAL STRUCTURE OF InGaN A Thesis Submitted to The Department of Physics and Applied Physics University of Strathclyde For the Degree of Doctor of Philosophy By Madeleine Eve White April 2002 DECLARATION OF AUTHOR’S RIGHTS The copyright of this thesis belongs to the author under the terms of the United Kingdom Copyright Acts as qualified by University of Strathclyde Regulation 3.49. Due acknowledgement must always be made of the use of any material contained in, or derived from, this thesis. ABSTRACT This thesis presents work on the optical properties, composition and local structure of InGaN. Low temperature photoluminescence (PL) spectroscopy of InGaN epilayers was used to study linewidth, intensity and uniformity of the samples. Temperature dependence of the PL spectrum showed a decrease in the peak emission energy with increasing temperature for one epilayer sample and an ‘S’- shape temperature dependence of peak emission energy for a second sample. Composition analysis of the epilayers using three different experimental techniques; Rutherford backscattering spectrometry (RBS), electron probe microanalysis (EPMA) and extended X-ray absorption fine structure (EXAFS), found that the indium nitride fraction varies linearly with the PL peak emission energy. Confocal microscopy of InGaN epilayer, quantum well (QW) and quantum box (QB) samples revealed that InGaN luminescence segregates into bright and dark spots, ~1m in diameter, and indicates that the origin of InGaN luminescence is of sub m-scale. InGaN local structure parameters were derived from EXAFS measurements. Epilayer samples exhibited a single-phase InGaN alloy while a QB sample showed a two-phase mixture of components, one of which is nearly pure InN.