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Brewing Yeast – Theory and Practice
Brewing yeast – theory and practice Chris Boulton Topics • What is brewing yeast? • Yeast properties, fermentation and beer flavour • Sources of yeast • Measuring yeast concentration The nature of yeast • Yeast are unicellular fungi • Characteristics of fungi: • Complex cells with internal organelles • Similar to plants but non-photosynthetic • Cannot utilise sun as source of energy so rely on chemicals for growth and energy Classification of yeast Kingdom Fungi Moulds Yeast Mushrooms / toadstools Genus > 500 yeast genera (Means “Sugar fungus”) Saccharomyces Species S. cerevisiae S. pastorianus (ale yeast) (lager yeast) Strains Many thousands! Biology of ale and lager yeasts • Two types indistinguishable by eye • Domesticated by man and not found in wild • Ale yeasts – Saccharomyces cerevisiae • Much older (millions of years) than lager strains in evolutionary terms • Lot of diversity in different strains • Lager strains – Saccharomyces pastorianus (previously S. carlsbergensis) • Comparatively young (probably < 500 years) • Hybrid strains of S. cerevisiae and wild yeast (S. bayanus) • Not a lot of diversity Characteristics of ale and lager yeasts Ale Lager • Often form top crops • Usually form bottom crops • Ferment at higher temperature o • Ferment well at low temperatures (18 - 22 C) (5 – 10oC) • Quicker fermentations (few days) • Slower fermentations (1 – 3 weeks) • Can grow up to 37oC • Cannot grow above 34oC • Fine well in beer • Do not fine well in beer • Cannot use sugar melibiose • Can use sugar melibiose Growth of yeast cells via budding + + + + Yeast cells • Each cell is ca 5 – 10 microns in diameter (1 micron = 1 millionth of a metre) • Cells multiply by budding a b c d h g f e Yeast and ageing - cells can only bud a certain number of times before death occurs. -
Multiple Genetic Origins of Saccharomyces Cerevisiae in Bakery
Multiple genetic origins of Saccharomyces cerevisiae in bakery Frédéric Bigey, Diego Segond, Lucie Huyghe, Nicolas Agier, Aurélie Bourgais, Anne Friedrich, Delphine Sicard To cite this version: Frédéric Bigey, Diego Segond, Lucie Huyghe, Nicolas Agier, Aurélie Bourgais, et al.. Multiple genetic origins of Saccharomyces cerevisiae in bakery. Comparative genomics of eukaryotic microbes: genomes in flux, and flux between genomes, Oct 2019, Sant Feliu de Guixol, Spain. hal-02950887 HAL Id: hal-02950887 https://hal.archives-ouvertes.fr/hal-02950887 Submitted on 28 Sep 2020 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. genetic diversity of229 propagating a natural sourdough, which is a of mixed naturally fermented domestication of wide diversity of fermented products like wine, Saccharomyces cerevisiae sake, beer, cocoa and bread. While bread is of cultural and historical importance, the highly diverse environments like human, wine, sake, fruits, tree, soil... fruits, tree, human, wine, sake, like highly diverse environments clade on the 1002 genomes whichtree, also includes beer strains. Sourdough strains mosaic are and genetically are to related strains from strains. Selection for strains commercial has maintained autotetraploid. strains mostly to Commercial are related strains of origin the Mixed The origin of bakery strains is polyphyletic. -
Relation Between the Recipe of Yeast Dough Dishes and Their Glycaemic Indices and Loads
foods Article Relation between the Recipe of Yeast Dough Dishes and Their Glycaemic Indices and Loads Ewa Raczkowska * , Karolina Ło´zna , Maciej Bienkiewicz, Karolina Jurczok and Monika Bronkowska Department of Human Nutrition, Faculty of Biotechnology and Food Sciences, Wrocław University of Environmental and Life Sciences, 51-630 Wroclaw, Poland * Correspondence: [email protected]; Tel.: +48-71-320-7726 Received: 23 July 2019; Accepted: 30 August 2019; Published: 1 September 2019 Abstract: The aim of the study was to evaluate the glycaemic indices (GI) and glycaemic loads (GL) of four food dishes made from yeast dough (steamed dumplings served with yoghurt, apple pancakes sprinkled with sugar powder, rolls with cheese and waffles with sugar powder), based on their traditional and modified recipes. Modification of the yeast dough recipe consisted of replacing wheat flour (type 500) with whole-wheat flour (type 2000). Energy value and the composition of basic nutrients were assessed for every tested dish. The study was conducted on 50 people with an average age of 21.7 1.1 years, and an average body mass index of 21.2 2.0 kg/m2. The GI of the analysed ± ± food products depended on the total carbohydrate content, dietary fibre content, water content, and energy value. Modification of yeast food products by replacing wheat flour (type 500) with whole-wheat flour (type 2000) contributed to the reduction of their GI and GL values, respectively. Keywords: glycaemic index; glycaemic load; yeast dough 1. Introduction In connection with the growing number of lifestyle diseases, consumers pay increasing attention to food, not only to food that have a better taste but also to food that help maintain good health. -
Sensory Evaluation and Staling of Bread Produced by Mixed Starter of Saccharomyces Cerevisiae and L.Plantarum
J. Food and Dairy Sci., Mansoura Univ., Vol. 5 (4): 221 - 233, 2014 Sensory evaluation and staling of bread produced by mixed starter of saccharomyces cerevisiae and l.plantarum Swelim, M. A. / Fardous M. Bassouny / S. A. El-Sayed / Nahla M. M. Hassan / Manal S. Ibrahim. * Botany Department, Faculty of science, Banha University. ** Agricultural Microbiology Department, Agricultural Research Center, Giza, Egypt. ***Food Technology Research Institute, Agricultural Research Center, Giza, Egypt. ABSTRACT Impact of processed conditions and type of starter cultivars on characteristics of wheat dough bread by using mixed starter cultivars of Sacch. cerevisiae and L. plantarum was estimated. L. plantarum seemed to be more effective in combination with Sacch. cerevisiae on the dough volume. Dough produced by starter containing L. plantarum characterized with lower PH and higher total titratable acidity (TTA) and moisture content, in comparison with control treatment. Highly significant differences in aroma and crumb texture were recorded with bread produced by starter culture containing L. plantarum. The obtained results also revealed significant differences in bread firmness which reflected the staling and its rate among the tested treatments after 1, 3, 5 and 6 days during storage time at room temperature. Data also confirmed a processing technique using L. planturium mixed with Sacch.cerevisia to enhance organoleptic properties of produced bread. INTRODUCTION Cereal fermentation is one of the oldest biotechnological processes, dating back to ancient Egypt, where both beer and bread were produced by using yeasts and lactic acid bacteria (LAB), Clarke and Arendt, (2005). Starters composed of specific individual LAB, or mixed with yeasts, became available a few years ago allowing the production of a full sourdough in one- stage process. -
AP-42, CH 9.13.4: Yeast Production
9.13.4 Yeast Production 9.13.4.1 General1 Baker’s yeast is currently manufactured in the United States at 13 plants owned by 6 major companies. Two main types of baker’s yeast are produced, compressed (cream) yeast and dry yeast. The total U. S. production of baker’s yeast in 1989 was 223,500 megagrams (Mg) (245,000 tons). Of the total production, approximately 85 percent of the yeast is compressed (cream) yeast, and the remaining 15 percent is dry yeast. Compressed yeast is sold mainly to wholesale bakeries, and dry yeast is sold mainly to consumers for home baking needs. Compressed and dry yeasts are produced in a similar manner, but dry yeasts are developed from a different yeast strain and are dried after processing. Two types of dry yeast are produced, active dry yeast (ADY) and instant dry yeast (IDY). Instant dry yeast is produced from a faster-reacting yeast strain than that used for ADY. The main difference between ADY and IDY is that ADY has to be dissolved in warm water before usage, but IDY does not. 9.13.4.2 Process Description1 Figure 9.13.4-1 is a process flow diagram for the production of baker’s yeast. The first stage of yeast production consists of growing the yeast from the pure yeast culture in a series of fermentation vessels. The yeast is recovered from the final fermentor by using centrifugal action to concentrate the yeast solids. The yeast solids are subsequently filtered by a filter press or a rotary vacuum filter to concentrate the yeast further. -
Impact of Wort Amino Acids on Beer Flavour: a Review
fermentation Review Impact of Wort Amino Acids on Beer Flavour: A Review Inês M. Ferreira and Luís F. Guido * LAQV/REQUIMTE, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, 687, 4169-007 Porto, Portugal; ines.fi[email protected] * Correspondence: [email protected]; Tel.: +351-220-402-644 Received: 3 March 2018; Accepted: 25 March 2018; Published: 28 March 2018 Abstract: The process by which beer is brewed has not changed significantly since its discovery thousands of years ago. Grain is malted, dried, crushed and mixed with hot water to produce wort. Yeast is added to the sweet, viscous wort, after which fermentation occurs. The biochemical events that occur during fermentation reflect the genotype of the yeast strain used, and its phenotypic expression is influenced by the composition of the wort and the conditions established in the fermenting vessel. Although wort is complex and not completely characterized, its content in amino acids indubitably affects the production of some minor metabolic products of fermentation which contribute to the flavour of beer. These metabolic products include higher alcohols, esters, carbonyls and sulfur-containing compounds. The formation of these products is comprehensively reviewed in this paper. Furthermore, the role of amino acids in the beer flavour, in particular their relationships with flavour active compounds, is discussed in light of recent data. Keywords: amino acids; beer; flavour; higher alcohols; esters; Vicinal Diketones (VDK); sulfur compounds 1. Introduction The process by which beer has been brewed has not changed significantly since its discovery over 2000 years ago. Although industrial equipment is used for modern commercial brewing, the principles are the same. -
Applicability of Yeast Fermentation to Reduce Fructans and Other Fodmaps
nutrients Article Applicability of Yeast Fermentation to Reduce Fructans and Other FODMAPs Vera Fraberger , Lisa-Maria Call, Konrad J. Domig and Stefano D’Amico * Department of Food Science and Technology, University of Natural Resources and Life Sciences (BOKU), 1190 Vienna, Austria; [email protected] (V.F.); [email protected] (L.-M.C.); [email protected] (K.J.D.) * Correspondence: [email protected]; Tel.: +43-1-47654-75243 Received: 13 August 2018; Accepted: 4 September 2018; Published: 6 September 2018 Abstract: A diet low in fermentable oligosaccharides, disaccharides, monosaccharides and, polyols (FODMAPs) is recommended for people affected by irritable bowel syndrome (IBS) and non-coeliac wheat sensitivity (NCWS) in order to reduce symptoms. Therefore, the aim of this study was to evaluate the impact of 13 sourdough-related yeasts on FODMAP degradation, especially fructans. First, a model system containing a typical wheat carbohydrate profile was applied to evaluate the growth rate of each yeast strain. Additionally, changes in the sugar composition, for up to four days, were monitored by high-pressure anion-exchange chromatography (HPAEC). A more realistic approach with a wheat flour suspension was used to characterize CO2 production according to the Einhorn method. The reduction of the total fructans was analyzed using an enzymatic method. Furthermore, a fingerprint of the present fructans with different degrees of polymerization was analyzed by HPAEC. The results revealed strong differences in the examined yeast strains’ ability to degrade fructans, in both the model system and wheat flour. Overall, Saccharomyces cerevisiae isolated from Austrian traditional sourdough showed the highest degree of degradation of the total fructan content and the highest gas building capacity, followed by Torulaspora delbrueckii. -
Use of Pseudocereals Preferment Made with Aromatic Yeast Strains for Enhancing Wheat Bread Quality
foods Article Use of Pseudocereals Preferment Made with Aromatic Yeast Strains for Enhancing Wheat Bread Quality Adriana Păucean 1 , Simona Maria Man 1,* , Maria Simona Chi¸s 1, Vlad Mure¸san 1 , Carmen Rodica Pop 2 , Sonia Ancu¸taSocaci 2 , Crina Carmen Mure¸san 1 and Sevasti¸taMuste 1 1 Department of Food Engineering, Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, 3-5, Manastur Street, 400372 Cluj-Napoca, Romania; [email protected] (A.P.); [email protected] (M.S.C.); [email protected] (V.M.); [email protected] (C.C.M.); [email protected] (S.M.) 2 Department of Food Science, Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, 3-5, Manastur Street, 400372 Cluj-Napoca, Romania; [email protected] (C.R.P); [email protected] (S.A.S) * Correspondence: [email protected]; Tel.: +40-264-596384 Received: 23 August 2019; Accepted: 24 September 2019; Published: 26 September 2019 Abstract: Usually, aromatic yeasts are designed to ferment wheat substrates for baking purposes but identification of new substrates for these strains and consequently new formulations for dough could lead to diversified bakery products with improved nutritional qualities and specific sensorial properties. The purpose of our study was to optimize the fermentation of quinoa and amaranth flours with non-conventional yeast strains in order to obtain a preferment with high potential in enhancing nutritional, textural and sensorial features of white wheat bread. Two biotypes of Saccharomyces cerevisiae yeast—a wine yeast strain and a beer yeast strain—commercialized for their aromatic properties were used. -
Mead Features Fermented by Saccharomyces Cerevisiae (Lalvin K1-1116)
African Journal of Biotechnology Vol. 12(2), pp. 199-204, 9 January, 2013 Available online at http://www.academicjournals.org/AJB DOI: 10.5897/AJB12.2147 ISSN 1684–5315 ©2013 Academic Journals Full Length Research Paper Mead features fermented by Saccharomyces cerevisiae (lalvin k1-1116) Eduardo Marin MORALES1*, Valmir Eduardo ALCARDE2 and Dejanira de Franceschi de ANGELIS1 1Department of Biochemistry and Microbiology, Institute of Biosciences, UNESP - Univ Estadual Paulista, Av. 24-A, 1515, 13506-900, Rio Claro, SP, Brazil. 2UNIMEP – Methodist University of Piracicaba. Rodovia Santa Bárbara Km 1, 13450-000 - Santa Barbara D'Oeste, SP, Brazil. Accepted 5 December, 2012 Alcoholic beverages are produced practically in every country in the world representing a significant percentage of the economy. Mead is one of the oldest beverages and it is easily obtained by the fermentation of a mixture of honey and water. However, it is still less studied compared to other beverages and does not have industrialized production. It is prepared as a handmade product. The origin of the honey used to formulate the mead creates differences on the final product characteristics. In this study, fermentation occurred at temperatures of about 22.1 ± 0.4°C after a previous pasteurization and inoculation. Saccharomyces cerevisiae (K1-LALVIN 1116) was used to produce the mead that was prepared in order to obtain dry mead by mixing 200 g L-1 of honey in water. For better -1 -1 results inorganic salts were used [(NH4)2SO4: 0.2 g L , (NH4)2HPO4: 0.02 g L ]. The results at the end of the process were a mead with: 12.5 ± 0.4°GL; pH 3.33; low amounts of high alcohols and methanol and great quantity of esters, that provide a nailing flavor to the beverage. -
Evaluation of Yeast and Lactic Acid Bacteria Starter Cultures for The
cess Pro ing d & o o T F e c f h Hassen et al., J Food Process Technol 2018, 9:3 o n l o a l n o r Journal of Food DOI: 10.4172/2157-7110.1000721 g u y o J Processing & Technology ISSN: 2157-7110 Research Article Open Access Evaluation of Yeast and Lactic Acid Bacteria Starter Cultures for the Production of Rice Injera Hassen Y1*, Mukisa IM2, Kurabachew H1 and Desalegn BB1 1School of Nutrition, Food Science and Technology, College of Agriculture, Hawassa University, Hawassa, Ethiopia 2Department of Food Technology and Nutrition, Makerere University, Kampala, Uganda Abstract Injera is a yeast-risen flat-bread with a unique, slightly spongy texture. It is a national staple in Ethiopia that is eaten daily in virtually every household. Despite the fact that injera is a favorite staple food, starters have not been evaluated for the production of rice-based injera. Combination of starter cultures of lactic acid (Lactobacillus plantarum, Lactobacillus fermentum) and yeast (Saccharomyces cerevisiae) and irsho (back slopping) were used to ferment rice batter during 96 h. Changes in pH, titratable acidity (TA) and microbial count were analyzed at 6 h intervals and consumer acceptability was done at 24 h and 48 h. LAB starters and their co-cultures yeast decreased pH from 6.35 to 4.5 and increased titratable acidity from 0.33% to 0.95% (lactic acid w/w ) within 18-24 h while in the spontaneous fermentation with traditional irsho it took 48 h-54 h to attain pH and titratable acidity value of 0.38% and 0.93%, respectively. -
Saccharomyces Cerevisiae in the Production of Fermented Beverages
beverages Review Saccharomyces cerevisiae in the Production of Fermented Beverages Graeme M Walker 1,* and Graham G Stewart 2 1 Abertay University, Dundee, Scotland DD1 1HG, UK 2 Heriot-Watt University, Edinburgh, Scotland EH14 4AS, UK; [email protected] * Correspondence: [email protected]; Tel.: +44-1382-308658 Academic Editor: Edgar Chambers IV Received: 20 October 2016; Accepted: 11 November 2016; Published: 17 November 2016 Abstract: Alcoholic beverages are produced following the fermentation of sugars by yeasts, mainly (but not exclusively) strains of the species, Saccharomyces cerevisiae. The sugary starting materials may emanate from cereal starches (which require enzymatic pre-hydrolysis) in the case of beers and whiskies, sucrose-rich plants (molasses or sugar juice from sugarcane) in the case of rums, or from fruits (which do not require pre-hydrolysis) in the case of wines and brandies. In the presence of sugars, together with other essential nutrients such as amino acids, minerals and vitamins, S. cerevisiae will conduct fermentative metabolism to ethanol and carbon dioxide (as the primary fermentation metabolites) as the cells strive to make energy and regenerate the coenzyme NAD+ under anaerobic conditions. Yeasts will also produce numerous secondary metabolites which act as important beverage flavour congeners, including higher alcohols, esters, carbonyls and sulphur compounds. These are very important in dictating the final flavour and aroma characteristics of beverages such as beer and wine, but also in distilled beverages such as whisky, rum and brandy. Therefore, yeasts are of vital importance in providing the alcohol content and the sensory profiles of such beverages. This Introductory Chapter reviews, in general, the growth, physiology and metabolism of S. -
INTERSPECIFIC HYBRID BETWEEN Saccharomyces Mikatae and Saccharomyces Cerevisiae AS an ALTERNATIVE STRAIN for BREAD MAKING
INTERSPECIFIC HYBRID BETWEEN Saccharomyces mikatae AND Saccharomyces cerevisiae AS AN ALTERNATIVE STRAIN FOR BREAD MAKING Yuji Oda1,2*, Subaru Tanizaki1, Megumi Yokoyama-Ohtsuka1, Hiroaki Sakurai2 Address(es): 1 Obihiro University of Agriculture and Veterinary Medicine, Department of Life and Agricultural Sciences, Obihiro, Hokkaido 080-8555, Japan. 2 Nippon Beet Sugar Manufacturing Co., Research Center, Obihiro, Hokkaido 080-0831, Japan. *Corresponding author: [email protected] doi: 10.15414/jmbfs.2020.10.1.127-129 ARTICLE INFO ABSTRACT Received 3. 9. 2019 Hybrids of the homothallic diploid Saccharomyces mikatae and heterothallic haploid Saccharomyces cerevisiae isolated from local Revised 2. 5. 2020 fruits in Hokkaido and a baking strain, respectively, were constructed by the spore to cell mating method. Interspecific hybridization of Accepted 22. 5. 2020 the selected strain was confirmed by polymerase chain reaction using species-specific primers, fermentation profiles, and spore Published 1. 8. 2020 formation. The leavening ability of the dough and enzyme activities of the hybrid exhibited intermediate traits of their parental strains. A baking test by the sponge-dough method with a standard formulation of white bread showed that breads produced using the hybrid differed in aroma and taste from those made using a conventional baking strain. Thus, the hybrid may be used to produce baked goods Short communication with diverse quality, which may be more acceptable to specific consumers. Keywords: Bread making, Hybrid, Saccharomyces mikatae, Saccharomyces cerevisiae INTRODUCTION During a survey of local genetic resources, we found a fermentative yeast strain, identified as S. mikatae, which was unsuitable for usual bread making as it Saccharomyces is a teleomorphic ascomycetous genus that generally exhibits exhibited a defect in maltose fermentation.