INTERSPECIFIC HYBRID BETWEEN Saccharomyces Mikatae and Saccharomyces Cerevisiae AS an ALTERNATIVE STRAIN for BREAD MAKING
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Brewing Yeast – Theory and Practice
Brewing yeast – theory and practice Chris Boulton Topics • What is brewing yeast? • Yeast properties, fermentation and beer flavour • Sources of yeast • Measuring yeast concentration The nature of yeast • Yeast are unicellular fungi • Characteristics of fungi: • Complex cells with internal organelles • Similar to plants but non-photosynthetic • Cannot utilise sun as source of energy so rely on chemicals for growth and energy Classification of yeast Kingdom Fungi Moulds Yeast Mushrooms / toadstools Genus > 500 yeast genera (Means “Sugar fungus”) Saccharomyces Species S. cerevisiae S. pastorianus (ale yeast) (lager yeast) Strains Many thousands! Biology of ale and lager yeasts • Two types indistinguishable by eye • Domesticated by man and not found in wild • Ale yeasts – Saccharomyces cerevisiae • Much older (millions of years) than lager strains in evolutionary terms • Lot of diversity in different strains • Lager strains – Saccharomyces pastorianus (previously S. carlsbergensis) • Comparatively young (probably < 500 years) • Hybrid strains of S. cerevisiae and wild yeast (S. bayanus) • Not a lot of diversity Characteristics of ale and lager yeasts Ale Lager • Often form top crops • Usually form bottom crops • Ferment at higher temperature o • Ferment well at low temperatures (18 - 22 C) (5 – 10oC) • Quicker fermentations (few days) • Slower fermentations (1 – 3 weeks) • Can grow up to 37oC • Cannot grow above 34oC • Fine well in beer • Do not fine well in beer • Cannot use sugar melibiose • Can use sugar melibiose Growth of yeast cells via budding + + + + Yeast cells • Each cell is ca 5 – 10 microns in diameter (1 micron = 1 millionth of a metre) • Cells multiply by budding a b c d h g f e Yeast and ageing - cells can only bud a certain number of times before death occurs. -
Molecular Evolution in Yeast: Role of Chromosomal Inversions and Translocations in Speciation, Adaptation and Gene Expression
Molecular evolution in yeast: Role of chromosomal inversions and translocations in speciation, adaptation and gene expression A thesis submitted to the University of Manchester for the degree of Doctor of Philosophy (PhD) in the Faculty of Life Sciences 2011 Samina Naseeb Table of Contents List of tables 7 List of figures 9 Abstract 12 Declaration 13 Copyright statement 14 Acknowledgements 15 Dedication 16 Rational for submitting the thesis in alternative format 17 Chapter 1- Introduction 18 1.1 Saccharomyces cerevisiae as a model organism 19 1.1.1 DNA transformation and recombination in yeast 19 1.1.2 Life cycle 20 1.1.3 Genome of Saccharomyces cerevisiae 23 1.2 The taxonomy of yeast 25 1.3 Yeast genome evolution 28 1.4 Transposable elements (TE) and chromosomal rearrangements 30 1.5 Role of chromosomal inversions and translocations in gene order 34 evolution, speciation and fitness 1.6 Co-expressed gene clusters 35 1.6.1 Structure of the DAL cluster 36 1.6.2 Function of DAL cluster in allantoin degradation 37 1.7 Aim of the project 40 1.8 References 41 Chapter 2- Impact of chromosomal inversions in co-expressed gene clusters 49 2.1 Foreword 50 2 2.2 Abstract 50 2.3 Background 51 2.4 Results 55 2.4.1 Transcription factor analysis of DAL cluster structure 55 2.4.2 Fitness assays of the wild type strains 59 2.4.3 Construction of the strains with different gene 62 inversions 2.4.4 Plasmid extraction and digestion 62 2.4.5 Amplification of cassettes 63 2.4.6 Construction of the DAL2 inverted strain 64 2.4.6.1 Insertion of the lox P containing cassettes 64 2.4.6.2 Verification of cassette insertion 65 2.4.6.3 Cre transformation and induction 67 2.4.7 Construction of the strain with S. -
WINE YEAST: the CHALLENGE of LOW TEMPERATURE Zoel Salvadó Belart Dipòsit Legal: T.1304-2013
WINE YEAST: THE CHALLENGE OF LOW TEMPERATURE Zoel Salvadó Belart Dipòsit Legal: T.1304-2013 ADVERTIMENT. L'accés als continguts d'aquesta tesi doctoral i la seva utilització ha de respectar els drets de la persona autora. Pot ser utilitzada per a consulta o estudi personal, així com en activitats o materials d'investigació i docència en els termes establerts a l'art. 32 del Text Refós de la Llei de Propietat Intel·lectual (RDL 1/1996). Per altres utilitzacions es requereix l'autorització prèvia i expressa de la persona autora. En qualsevol cas, en la utilització dels seus continguts caldrà indicar de forma clara el nom i cognoms de la persona autora i el títol de la tesi doctoral. No s'autoritza la seva reproducció o altres formes d'explotació efectuades amb finalitats de lucre ni la seva comunicació pública des d'un lloc aliè al servei TDX. Tampoc s'autoritza la presentació del seu contingut en una finestra o marc aliè a TDX (framing). Aquesta reserva de drets afecta tant als continguts de la tesi com als seus resums i índexs. ADVERTENCIA. El acceso a los contenidos de esta tesis doctoral y su utilización debe respetar los derechos de la persona autora. Puede ser utilizada para consulta o estudio personal, así como en actividades o materiales de investigación y docencia en los términos establecidos en el art. 32 del Texto Refundido de la Ley de Propiedad Intelectual (RDL 1/1996). Para otros usos se requiere la autorización previa y expresa de la persona autora. En cualquier caso, en la utilización de sus contenidos se deberá indicar de forma clara el nombre y apellidos de la persona autora y el título de la tesis doctoral. -
Genome Diversity and Evolution in the Budding Yeasts (Saccharomycotina)
| YEASTBOOK GENOME ORGANIZATION AND INTEGRITY Genome Diversity and Evolution in the Budding Yeasts (Saccharomycotina) Bernard A. Dujon*,†,1 and Edward J. Louis‡,§ *Department Genomes and Genetics, Institut Pasteur, Centre National de la Recherche Scientifique UMR3525, 75724-CEDEX15 Paris, France, †University Pierre and Marie Curie UFR927, 75005 Paris, France, ‡Centre for Genetic Architecture of Complex Traits, and xDepartment of Genetics, University of Leicester, LE1 7RH, United Kingdom ORCID ID: 0000-0003-1157-3608 (E.J.L.) ABSTRACT Considerable progress in our understanding of yeast genomes and their evolution has been made over the last decade with the sequencing, analysis, and comparisons of numerous species, strains, or isolates of diverse origins. The role played by yeasts in natural environments as well as in artificial manufactures, combined with the importance of some species as model experimental systems sustained this effort. At the same time, their enormous evolutionary diversity (there are yeast species in every subphylum of Dikarya) sparked curiosity but necessitated further efforts to obtain appropriate reference genomes. Today, yeast genomes have been very informative about basic mechanisms of evolution, speciation, hybridization, domestication, as well as about the molecular machineries underlying them. They are also irreplaceable to investigate in detail the complex relationship between genotypes and phenotypes with both theoretical and practical implications. This review examines these questions at two distinct levels offered by the broad evolutionary range of yeasts: inside the best-studied Saccharomyces species complex, and across the entire and diversified subphylum of Saccharomycotina. While obviously revealing evolutionary histories at different scales, data converge to a remarkably coherent picture in which one can estimate the relative importance of intrinsic genome dynamics, including gene birth and loss, vs. -
Multiple Genetic Origins of Saccharomyces Cerevisiae in Bakery
Multiple genetic origins of Saccharomyces cerevisiae in bakery Frédéric Bigey, Diego Segond, Lucie Huyghe, Nicolas Agier, Aurélie Bourgais, Anne Friedrich, Delphine Sicard To cite this version: Frédéric Bigey, Diego Segond, Lucie Huyghe, Nicolas Agier, Aurélie Bourgais, et al.. Multiple genetic origins of Saccharomyces cerevisiae in bakery. Comparative genomics of eukaryotic microbes: genomes in flux, and flux between genomes, Oct 2019, Sant Feliu de Guixol, Spain. hal-02950887 HAL Id: hal-02950887 https://hal.archives-ouvertes.fr/hal-02950887 Submitted on 28 Sep 2020 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. genetic diversity of229 propagating a natural sourdough, which is a of mixed naturally fermented domestication of wide diversity of fermented products like wine, Saccharomyces cerevisiae sake, beer, cocoa and bread. While bread is of cultural and historical importance, the highly diverse environments like human, wine, sake, fruits, tree, soil... fruits, tree, human, wine, sake, like highly diverse environments clade on the 1002 genomes whichtree, also includes beer strains. Sourdough strains mosaic are and genetically are to related strains from strains. Selection for strains commercial has maintained autotetraploid. strains mostly to Commercial are related strains of origin the Mixed The origin of bakery strains is polyphyletic. -
Relation Between the Recipe of Yeast Dough Dishes and Their Glycaemic Indices and Loads
foods Article Relation between the Recipe of Yeast Dough Dishes and Their Glycaemic Indices and Loads Ewa Raczkowska * , Karolina Ło´zna , Maciej Bienkiewicz, Karolina Jurczok and Monika Bronkowska Department of Human Nutrition, Faculty of Biotechnology and Food Sciences, Wrocław University of Environmental and Life Sciences, 51-630 Wroclaw, Poland * Correspondence: [email protected]; Tel.: +48-71-320-7726 Received: 23 July 2019; Accepted: 30 August 2019; Published: 1 September 2019 Abstract: The aim of the study was to evaluate the glycaemic indices (GI) and glycaemic loads (GL) of four food dishes made from yeast dough (steamed dumplings served with yoghurt, apple pancakes sprinkled with sugar powder, rolls with cheese and waffles with sugar powder), based on their traditional and modified recipes. Modification of the yeast dough recipe consisted of replacing wheat flour (type 500) with whole-wheat flour (type 2000). Energy value and the composition of basic nutrients were assessed for every tested dish. The study was conducted on 50 people with an average age of 21.7 1.1 years, and an average body mass index of 21.2 2.0 kg/m2. The GI of the analysed ± ± food products depended on the total carbohydrate content, dietary fibre content, water content, and energy value. Modification of yeast food products by replacing wheat flour (type 500) with whole-wheat flour (type 2000) contributed to the reduction of their GI and GL values, respectively. Keywords: glycaemic index; glycaemic load; yeast dough 1. Introduction In connection with the growing number of lifestyle diseases, consumers pay increasing attention to food, not only to food that have a better taste but also to food that help maintain good health. -
Interspecific Hybrids Reveal Increased Fermentation
fermentation Article Saccharomyces arboricola and Its Hybrids’ Propensity for Sake Production: Interspecific Hybrids Reveal Increased Fermentation Abilities and a Mosaic Metabolic Profile Matthew J. Winans 1,2,* , Yuki Yamamoto 1, Yuki Fujimaru 1, Yuki Kusaba 1, Jennifer E. G. Gallagher 2 and Hiroshi Kitagaki 1 1 Graduate School of Advanced Health Sciences, Saga University, 1, Honjo, Saga city, Saga 840-8502, Japan; [email protected] (Y.Y.); [email protected] (Y.F.); [email protected] (Y.K.); [email protected] (H.K.) 2 Biology Department, West Virginia University, 53 Campus Drive, Morgantown, WV 26506-6057, USA; [email protected] * Correspondence: [email protected]; Tel.: +1-(304)-483-1786; Fax: +1-(304)-293-6363 Received: 4 December 2019; Accepted: 17 January 2020; Published: 20 January 2020 Abstract: The use of interspecific hybrids during the industrial fermentation process has been well established, positioning the frontier of advancement in brewing to capitalize on the potential of Saccharomyces hybridization. Interspecific yeast hybrids used in modern monoculture inoculations benefit from a wide range of volatile metabolites that broaden the organoleptic complexity. This is the first report of sake brewing by Saccharomyces arboricola and its hybrids. S. arboricola x S. cerevisiae direct-mating generated cryotolerant interspecific hybrids which increased yields of ethanol and ethyl hexanoate compared to parental strains, important flavor attributes of fine Japanese ginjo sake rice wine. Hierarchical clustering heatmapping with principal component analysis for metabolic profiling was used in finding low levels of endogenous amino/organic acids clustered S. arboricola apart from the S. -
Sensory Evaluation and Staling of Bread Produced by Mixed Starter of Saccharomyces Cerevisiae and L.Plantarum
J. Food and Dairy Sci., Mansoura Univ., Vol. 5 (4): 221 - 233, 2014 Sensory evaluation and staling of bread produced by mixed starter of saccharomyces cerevisiae and l.plantarum Swelim, M. A. / Fardous M. Bassouny / S. A. El-Sayed / Nahla M. M. Hassan / Manal S. Ibrahim. * Botany Department, Faculty of science, Banha University. ** Agricultural Microbiology Department, Agricultural Research Center, Giza, Egypt. ***Food Technology Research Institute, Agricultural Research Center, Giza, Egypt. ABSTRACT Impact of processed conditions and type of starter cultivars on characteristics of wheat dough bread by using mixed starter cultivars of Sacch. cerevisiae and L. plantarum was estimated. L. plantarum seemed to be more effective in combination with Sacch. cerevisiae on the dough volume. Dough produced by starter containing L. plantarum characterized with lower PH and higher total titratable acidity (TTA) and moisture content, in comparison with control treatment. Highly significant differences in aroma and crumb texture were recorded with bread produced by starter culture containing L. plantarum. The obtained results also revealed significant differences in bread firmness which reflected the staling and its rate among the tested treatments after 1, 3, 5 and 6 days during storage time at room temperature. Data also confirmed a processing technique using L. planturium mixed with Sacch.cerevisia to enhance organoleptic properties of produced bread. INTRODUCTION Cereal fermentation is one of the oldest biotechnological processes, dating back to ancient Egypt, where both beer and bread were produced by using yeasts and lactic acid bacteria (LAB), Clarke and Arendt, (2005). Starters composed of specific individual LAB, or mixed with yeasts, became available a few years ago allowing the production of a full sourdough in one- stage process. -
AP-42, CH 9.13.4: Yeast Production
9.13.4 Yeast Production 9.13.4.1 General1 Baker’s yeast is currently manufactured in the United States at 13 plants owned by 6 major companies. Two main types of baker’s yeast are produced, compressed (cream) yeast and dry yeast. The total U. S. production of baker’s yeast in 1989 was 223,500 megagrams (Mg) (245,000 tons). Of the total production, approximately 85 percent of the yeast is compressed (cream) yeast, and the remaining 15 percent is dry yeast. Compressed yeast is sold mainly to wholesale bakeries, and dry yeast is sold mainly to consumers for home baking needs. Compressed and dry yeasts are produced in a similar manner, but dry yeasts are developed from a different yeast strain and are dried after processing. Two types of dry yeast are produced, active dry yeast (ADY) and instant dry yeast (IDY). Instant dry yeast is produced from a faster-reacting yeast strain than that used for ADY. The main difference between ADY and IDY is that ADY has to be dissolved in warm water before usage, but IDY does not. 9.13.4.2 Process Description1 Figure 9.13.4-1 is a process flow diagram for the production of baker’s yeast. The first stage of yeast production consists of growing the yeast from the pure yeast culture in a series of fermentation vessels. The yeast is recovered from the final fermentor by using centrifugal action to concentrate the yeast solids. The yeast solids are subsequently filtered by a filter press or a rotary vacuum filter to concentrate the yeast further. -
14419802.Pdf
View metadata, citation and similar papers at core.ac.uk brought to you by CORE REVIEW ARTICLE 277 Potential Application of Yeast β-Glucans in Food Industry Vesna ZECHNER-KRPAN 1 Vlatka PETRAVIĆ-TOMINAC 1 ( ) Ines PANJKOTA-KRBAVČIĆ 2 Slobodan GRBA 3 Katarina BERKOVIĆ 4 Summary Diff erent β-glucans are found in a variety of natural sources such as bacteria, yeast, algae, mushrooms, barley and oat. Th ey have potential use in medicine and pharmacy, food, cosmetic and chemical industries, in veterinary medicine and feed production. Th e use of diff erent β-glucans in food industry and their main characteristics important for food production are described in this paper. Th is review focuses on benefi cial properties and application of β-glucans isolated from diff erent yeasts, especially those that are considered as waste from brewing industry. Spent brewer’s yeast, a by-product of beer production, could be used as a raw-material for isolation of β-glucan. In spite of the fact that large quantities of brewer’s yeast are used as a feedstuff , certain quantities are still treated as a liquid waste. β-Glucan is one of the compounds that can achieve a greater commercial value than the brewer’s yeast itself and maximize the total profi tability of the brewing process. β-Glucan isolated from spent brewer’s yeast possesses properties that are benefi cial for food production. Th erefore, the use of spent brewer’s yeast for isolation of β-glucan intended for food industry would represent a payable technological and economical choice for breweries. -
Impact of Wort Amino Acids on Beer Flavour: a Review
fermentation Review Impact of Wort Amino Acids on Beer Flavour: A Review Inês M. Ferreira and Luís F. Guido * LAQV/REQUIMTE, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, 687, 4169-007 Porto, Portugal; ines.fi[email protected] * Correspondence: [email protected]; Tel.: +351-220-402-644 Received: 3 March 2018; Accepted: 25 March 2018; Published: 28 March 2018 Abstract: The process by which beer is brewed has not changed significantly since its discovery thousands of years ago. Grain is malted, dried, crushed and mixed with hot water to produce wort. Yeast is added to the sweet, viscous wort, after which fermentation occurs. The biochemical events that occur during fermentation reflect the genotype of the yeast strain used, and its phenotypic expression is influenced by the composition of the wort and the conditions established in the fermenting vessel. Although wort is complex and not completely characterized, its content in amino acids indubitably affects the production of some minor metabolic products of fermentation which contribute to the flavour of beer. These metabolic products include higher alcohols, esters, carbonyls and sulfur-containing compounds. The formation of these products is comprehensively reviewed in this paper. Furthermore, the role of amino acids in the beer flavour, in particular their relationships with flavour active compounds, is discussed in light of recent data. Keywords: amino acids; beer; flavour; higher alcohols; esters; Vicinal Diketones (VDK); sulfur compounds 1. Introduction The process by which beer has been brewed has not changed significantly since its discovery over 2000 years ago. Although industrial equipment is used for modern commercial brewing, the principles are the same. -
Brewing Efficacy of Non-Conventional Saccharomyces Non-Cerevisiae Yeasts Authors: James Bruner 1,2 (Orcid: 0000-0003-3691-0711)
Preprints (www.preprints.org) | NOT PEER-REVIEWED | Posted: 19 July 2021 doi:10.20944/preprints202107.0423.v1 Brewing Efficacy of Non-Conventional Saccharomyces Non-Cerevisiae Yeasts Authors: James Bruner 1,2 (ORCiD: 0000-0003-3691-0711), Andrew Marcus 1, Glen Fox 1* (ORCiD: 0000-0001-7502-0637) Addresses: 1. Food Science and Technology Department, University of California, 1 Shields Ave, Davis, CA, USA 95616 2. Creature Comforts Brewing Company, 271 W. Hancock Ave, Athens, GA, USA 30601 *Corresponding authors: [email protected] © 2021 by the author(s). Distributed under a Creative Commons CC BY license. Preprints (www.preprints.org) | NOT PEER-REVIEWED | Posted: 19 July 2021 doi:10.20944/preprints202107.0423.v1 Abstract Consumer demands for new sensory experiences have driven the research of unconventional yeasts in beer. While much research exists on the use of various common Saccharomyces cerevisiae strains as well as non-Saccharomyces yeasts, there exists a gap in knowledge regarding other non-cerevisiae Saccharomyces species in the fermentation of beer, outside that of S. pastorianus. Here, five distinct species of Saccharomyces from the UC Davis Phaff Yeast Culture Collection, as well as one interspecies hybrid from Fermentis, were chosen to ferment 40 L pilot scale beers. S. kudriavzevii, S. mikatae, S. paradoxus, S. bayanus, and S. uvarum yeasts were fermented in duplicate, with one fermenter in each pair receiving 10 g/L dry- hop during fermentation. Analytical measurements were made each day of fermentation and compared to controls of SafAle US-05 and SafLager W 34/70 for commercial brewing parameters of interest. Finished beers were also analyzed for aroma, taste, and mouthfeel to determine the flavor of each yeast as it pertains to brewing potential.