Influenza Hemagglutinin (HA) Peptide

Product Number I2149 Storage Temperature –20 C

CAS RN 92000-76-5 Storage/Stability Synonyms: HA tag peptide, HA1 fragment 98-106 Store lyophilized reagent at –20 C. For extended storage after reconstitution, store at –20 C in working Product Description aliquots. Avoid repeated freeze-thaw. Influenza Hemagglutinin (HA) peptide is a synthetic peptide with a molecular weight of 1102.15. The amino Product Profile acid sequence is: N-Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr- A working concentration of 5-10 g/ml is commonly Ala-C, which corresponds to amino acids 98–106 of used to compete out the binding of Monoclonal anti-HA human influenza virus hemagglutinin (HA). (Product Number H9658) and Rabbit anti-HA (Product Number H6908) antibodies at working dilution, in The peptide is useful for competing out anti-HA immuno-blotting assays. Preincubation of the peptide antibody binding to HA-tagged fusion proteins in with the antibody is recommended. immunoassays. The successful inhibition of antibody binding ascertains that the experimental observed A working concentration of 100 g/ml is commonly binding is specific. used to elute HA-tagged fusion proteins from an affinity column of Monoclonal Anti-HA agarose (Product The product is supplied as lyophilized powder. Number A2095). Elute the bound fusion protein by

adding five 1 column volume aliquots of a solution Precautions and Disclaimer containing 100 g/ml of this peptide in phosphate For R&D use only. Not for drug, household, or other buffered saline (PBS). uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling Note: In order to obtain the best results in various practices. techniques and preparations, it is recommended to

determine the optimal working dilution by titration. Preparation Instructions

To prepare a stock solution, dissolve in water to a final SM,IRB,VLE,MAM 01/19-1 concentration of 5 mg/ml. Aliquot and store at –20 C.

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